Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.7783507
Thus , our results indicate that RANK interacts with various TRAFs through distinct motifs and activates NF kappaB via a novel TRAF 6 interaction motif , which then activates NIK , thus leading to NF kappaB activation , whereas RANK most likely activates JNK through a TRAF 2 interacting region in RANK . . 0.7783507^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.60890933
Suppression of interaction of RANK with TRAF 6 by TRAF 6 binding peptide abrogated the anti proliferative effects of RANKL , suggesting the critical role of TRAF 6 . 0.60890933^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
From this deletion analysis , we determined that TRAF 2 , TRAF 5 , and TRAF 6 interact with RANK at its C terminal 85 amino acid tail ; the binding affinity appeared to be in the order of TRAF 2 > TRAF 5 > TRAF 6 . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
When this membrane proximal TRAF 6 interaction domain was deleted , RANK mediated NF kappaB signaling was completely inhibited while c Jun NH 2 terminal kinase activation was partially inhibited . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
IFN gamma induces rapid degradation of the RANK adapter protein , TRAF 6 ( tumour necrosis factor receptor associated factor 6 ) , which results in strong inhibition of the RANKL induced activation of the transcription factor NF kappaB and JNK . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
We recently demonstrated that TRANCE activates Akt via a mechanism involving TRANCE receptor ( TRANCE R ) / RANK , TRAF 6 , and c Src . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
TIZ expression also inhibited the signaling of RANK ( receptor activator of NF kappa B ) , which together with TRAF 6 has been shown to be essential for osteoclastogenesis . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Receptor activator of NF kappaB ligand ( RANKL ) activates TAK 1 mitogen activated protein kinase kinase kinase through a signaling complex containing RANK , TAB 2 , and TRAF 6 . ^^^ In 293 cells stably transfected with full length RANK , RANKL stimulation facilitates the formation of a complex containing RANK , TRAF 6 , TAB 2 , and TAK 1 , leading to the activation of TAK 1 . ^^^ These results suggest that the formation of the TRAF 6 TAB2 TAK 1 complex is involved in the RANK signaling pathway and may regulate the development and function of osteoclasts . . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
This cytokine induces rapid degradation of the RANK ( receptor activator of nuclear factor kappaB ) adapter protein TRAF 6 ( TNF receptor associated factor 6 ) , resulting in strong inhibition of the RANKL induced activation of NF kappaB and JNK ( c Jun N terminal kinase ) . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Here we report crystal structures of TRAF 6 , alone and in complex with TRAF 6 binding peptides from CD 40 and TRANCE R ( also known as RANK ) , members of the TNFR superfamily , to gain insight into the mechanism by which TRAF 6 mediates several signalling cascades . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
In contrast , the interaction of RANK with TRAF 6 is absolutely required for the proper formation of cytoskeletal structures and functional resorptive activity of osteoclasts . ^^^ Moreover , signaling via the interleukin 1 receptor , which also utilizes TRAF 6 , rescues the osteoclast activation defects observed in the absence of RANK / TRAF6 interactions . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
IL 7R ( alpha ) ( + ) cells harvested from TRAF 6 ( / ) embryos expressed LTalphabeta in response to IL 7 but not RANKL , demonstrating that the RANK TRAF 6 signaling pathway regulates LT ( alpha ) beta expression in LN but not in PP . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Disruption of rafts blocked TRAF 6 translocation and Akt activation by RANK ligand in osteoclasts and further reduced the survival of osteoclasts . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Furthermore compared with controls , RANKL induces enhanced association of TRAF 6 and RANK in both RAW264 . 7 cells expressing SHP 1 ( C453S ) and bone marrow macrophages from Me ( 5 ) / Me ( 5 ) mice . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Lipid rafts are important for the association of RANK and TRAF 6 . ^^^ We found that the majority of the ectopically expressed RANK and substantial portion of endogenous TRAF 2 and TRAF 6 were detected in the low density raft fractions . ^^^ The disruption of rafts blocked the TRAF 6 translocation by RANK ligand and impeded the interaction between RANK and TRAF 6 . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
When overexpressed , PIAS 3 inhibits NF kappaB dependent transcription induced by treatment with tumor necrosis factor alpha ( TNF alpha ) or interleukin 1beta or by overexpression of TNF family receptors such as RANK , TNFR 1 , and CD 30 or signal transducers of TNF receptor associated factors ( TRAFs ) , including TRAF 2 , TRAF 5 , and TRAF 6 . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
We also found that tumor necrosis factor ( TNF ) receptor associated factor 6 ( TRAF 6 ) , which mediates RANKL signaling , was constitutively bound to RANK in TNF receptor deleted cells but not in wild type cells , and this binding was enhanced by RANKL . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Semiquantitative RT PCR was performed to examine the effect of PAR on gene expression of RANK and TRAF 6 . ^^^ In addition , PAR inhibited NF kappaB activation induced by osteoclastogenic factors RANKL , interleukin ( IL ) 1beta , or TNF alpha to varying degrees and reduced the gene expression of RANK and TRAF 6 . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
RANK mediated amplification of TRAF 6 signaling leads to NFATc 1 induction during osteoclastogenesis . ^^^ RANK and CD 40 activate NF kappaB and MAPKs to similar levels via TRAF 6 . ^^^ Even though overexpression of TRAF 6 results in osteoclast formation , RANK but not CD 40 promotes osteoclastogenesis . ^^^ By introducing mutations into three TRAF 6 binding sites of RANK , we found that h40 / mRK with a single TRAF 6 binding site efficiently induced Ca2+ oscillation and expression of NFATc 1 , a master switch in osteoclastogenesis , whereas CD 40 carrying a single TRAF 6 binding site did not . ^^^ However , expression of CD 40 that was approximately 100 times greater than that of h40 / mRK resulted in osteoclast formation , indicating that the RANK TRAF 6 signal is more potent than the CD 40 TRAF6 signal in terms of NFATc 1 activation and osteoclastogenesis . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
RT PCR was used to measure the expression of RANK , matrix metalloproteinase 9 , TRAF 6 , TRAP , integrin alpha 5 , integrin beta 3 , cathepsin K , and carbonic anhydrase 2 ( CA 2 ) . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
There was no difference in RANK and osteoprotegerin expression between iNOS KO and WT bone marrow cultures after M CSF and RANKL treatment , while Traf 6 expression was significantly lower in the absence of iNOS . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Osteoclast differentiation independent of the TRANCE RANK TRAF 6 axis . ^^^ Hence , the current paradigm holds that TRANCE RANK interaction and subsequent signaling via TRAF 6 are essential for the generation of functional osteoclasts . ^^^ Surprisingly , we show that hematopoietic precursors from TRANCE , RANK , or TRAF 6 null mice can become osteoclasts in vitro when they are stimulated with TNF alpha in the presence of cofactors such as TGF beta . ^^^ We provide direct evidence against the current paradigm that the TRANCE RANK TRAF 6 pathway is essential for osteoclast differentiation and suggest the potential existence of alternative routes for osteoclast differentiation . . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
TNF receptor associated factor 6 ( TRAF 6 ) associates with the cytoplasmic domain of receptor activator of NF kappaB ( RANK ) . ^^^ TNF receptor associated factor 6 ( TRAF 6 ) associates with the cytoplasmic domain of receptor activator of NF kappaB ( RANK ) . ^^^ FHL 2 inhibits TRAF 6 induced NF kappaB activity in wild type osteoclast precursors and , in keeping with its role as a suppressor of TRAF 6 mediated RANK signaling , TRAF6 / RANK association is enhanced in FHL 2 / osteoclasts . ^^^ FHL 2 is , therefore , an antiosteoclastogenic molecule exerting its effect by attenuating TRAF 6 mediated RANK signaling . . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Anti osteoclastogenic properties of hemin were based on a down regulation of c fms , RANK , TRAF 6 , and c fos . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
To study the involvement of TRAF 6 in RANK induced osteoclastogenesis , osteoclast progenitors , in which RANK was overexpressed , were cultured with M CSF and TNF receptor associated factor ( TRAF ) 6 decoy peptides ( T6DP ) that inhibit the interaction of RANK with TRAF 6 . ^^^ The five C terminal mutants had the TRAF 6 binding domain in their cytoplasmic regions , suggesting that ligand independent osteoclastogenesis requires the receptor oligomerization of RANK . . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
In addition , RANK , TRAF 2 , TRAF 6 , and LacCer were found localized in lipid rafts on the cell surfaces . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Coimmunoprecipitation experiments suggested potential interactions between the cytoplasmic tail of TRANCE R with TRAF 1 , TRAF 2 , TRAF 3 , TRAF 5 , and TRAF 6 . ^^^ Dominant negative forms of TRAF 2 , TRAF 5 , and TRAF 6 and an endogenous inhibitor of TRAF 2 , TRAF interacting protein ( TRIP ) , substantially inhibited TRANCE R mediated NF kappaB activation , suggesting a role of TRAFs in regulating DC and osteoclast function . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
A deficiency in c Src or addition of Src family kinase inhibitors blocks TRANCE mediated PKB activation in osteoclasts . c Src and TRAF 6 interact with each other and with TRANCE R upon receptor engagement . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
Only the TRANCE receptor ( TRANCE R ) has been shown to induce osteoclastogenesis , even though other immune receptors , including CD 40 and IL 1R / Toll like receptor , use TRAF 6 to activate overlapping signalling cascades . ^^^ Our results suggest that differences in the osteoclastogenesis inducing capacity of TRANCE R versus other TRAF 6 associated receptors may in part stem from a quantitative difference in the TRAF 6 mediated signals . . ^^^
Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9Y6Q6 and Q9Y4K3	Pubmed	SVM Score :0.0
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