| NEMO / IKKgamma deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF kappaB DNA binding activity in response to TNFalpha , IL 1 , LPS , and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity , which correlates with a lack of phosphorylation and degradation of IkappaBalpha . ^^^ NEMO / IKKgamma deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF kappaB DNA binding activity in response to TNFalpha , IL 1 , LPS , and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity , which correlates with a lack of phosphorylation and degradation of IkappaBalpha . ^^^ |