| Interacting proteins: Q06330 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Protein protein interaction assays demonstrated contacts between SKIP and the SMRT , CIR , Sin3A , and HDAC 2 proteins of the CBF 1 corepressor complex . ^^^ |
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| Interacting proteins: Q06330 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| CBF 1 mediates transcriptional repression through the recruitment of an SMRT histone deacetylase containing corepressor complex . ^^^ In competition binding experiments SMRT displaced NotchIC from CBF 1 and from SKIP . ^^^ The results suggest a model in which NotchIC activates responsive promoters by competing with the SMRT corepressor complex for contacts on both CBF 1 and SKIP . . ^^^ |
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| Interacting proteins: Q06330 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Nuclear localization of CBF 1 is regulated by interactions with the SMRT corepressor complex . ^^^ In mammalian and yeast two hybrid assays , these mutations were also associated with a loss of CBF 1 mediated transcriptional repression and a severely impaired interaction with the corepressors SMRT and CIR . ^^^ Overexpression of SMRT rescued the ability of mutant CBF 1 to target to the nucleus of transfected cells and similarly rescued nuclear targeting of enhanced green fluorescent protein ( EGFP ) CBF 1 exons 6 to 9 CBF 1 ( 6 9 ) carrying the codon 233 or 249 mutations . ^^^ Carboxy terminally truncated SMRT with amino acids ( aa ) 1291 to 1495 deleted was unable to rescue the nuclear targeting of mutant EGFP CBF 1 ( 6 9 ) . ^^^ Comparison of the intracellular localization of CBF 1 ( 6 9 ) with that of CBF 1 ( 5 9 ) further supported the suggestion that nuclear targeting of CBF 1 is dependent on the formation of a CBF 1 SMRT SKIP corepressor complex . ^^^ |
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| Interacting proteins: Q06330 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Synaptic activity , acting via the nuclear Ca ( 2+ ) dependent activation of CaM kinase 4 , triggers the disruption of subnuclear domains containing class 2 histone deacetylases ( HDACs ) and silencing mediator of retinoic acid and thyroid hormone receptors ( SMRT ) , a broad specificity co repressor which represses nuclear hormone receptors and CBF 1 . ^^^ Counterbalancing these changes , protein phosphatase 1 promotes nuclear localization of SMRT and inactivation of nuclear receptors and CBF 1 . ^^^ |
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| Interacting proteins: Q06330 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| In cotransfection experiments , SHARP mediated repression was sensitive to the HDAC inhibitor TSA and facilitated by SKIP , a highly conserved SMRT and RBP Jkappa interacting protein . ^^^ |
|
| Interacting proteins: Q06330 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
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