| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| Here we show that the ability of the antiandrogen cyproterone acetate ( CPA ) to inhibit transactivation by the human AR ( hAR ) involves the corepressor SMRT ( silencing mediator for retinoic acid and thyroid hormone receptor ) . ^^^ In addition , we have used receptor point mutants that exhibit normal transactivation potential and unchanged partial agonistic activity when treated with CPA , but lack both SMRT binding and SMRT mediated inhibition of CPA bound AR . ^^^ Furthermore , we show that treatment of transfected cells with a cAMP analog or coexpression of the catalytic subunit of PKA , known to activate hAR , inhibits the binding of SMRT to the AR . ^^^ |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| Regulation of androgen receptor activity by the nuclear receptor corepressor SMRT . ^^^ In this study , we have investigated the regulation of AR transcriptional activity by the silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) . ^^^ We found that AR possesses an intrinsic transcriptional repression activity , and AR interacts directly with SMRT . ^^^ Overexpression of SMRT inhibits dihydrotestosterone dependent transactivation by AR and further suppresses the antiandrogen flutamide mediated inhibition of AR activity . ^^^ We provide evidence to suggest that the mechanisms of SMRT mediated inhibition of AR activity involves inhibition of AR N / C interaction and competition with the p 160 coactivator . ^^^ |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| In this study we assessed the effect of SMRT and DAX 1 on AR and PR activity in the presence of both agonists and partial antagonists . ^^^ |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| Here we show by a modified mammalian two hybrid system that both the AR interacting domains of the coactivator SRC 1 and of the corepressor SMRT compete for interaction with the AR N terminus . ^^^ We show that the Qr region of SRC 1 is able to inhibit the interaction of SMRT with AR . ^^^ |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| The androgen receptor ( AR ) activates target gene expression in the presence of agonist ligands via the recruitment of transcriptional coactivators , but recent work shows that overexpression of the nuclear corepressors NCoR and SMRT attenuates this agonist mediated AR activation . ^^^ |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| On the other hand , although overexpression of corepressors N CoR and SMRT could result in evident inhibition on DHT or CPA induced transactivity of wtAR and the AR mutants , N CoR displayed stronger inhibitory effects on DHT induced transactivity of the AR mutants ( especially for E872Q and M886I ) than that of wtAR . ^^^ |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We found that the gene expression level of FHL 2 decreased in prostate cancer compared with that of normal tissue and the gene expressions of PSA , AR and SMRT were not significant . ^^^ The correlation coefficient analysis revealed that strong associations were found in the pairs of AR versus SMRT , AR versus FHL 2 and SMRT versus FHL 2 in prostate cancer , whereas similarity was found only in the pair of AR versus FHL 2 in normal tissue . ^^^ |
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| Interacting proteins: Q9Y618 and P10275 |
Pubmed |
SVM Score :0.0 |
| We have investigated the role of corepressors SMRT ( silencing mediator of retinoid and thyroid hormone receptor ) and N CoR ( nuclear receptor corepressor ) in transcriptional regulation by androgen receptor ( AR ) in the LNCaP prostate cancer cell line . ^^^ Using specific small interference RNAs to knock down SMRT and / or N CoR in LNCaP cells , we found that SMRT and N CoR not only mediate antagonist dependent inhibition of AR activation but also have a widespread role in suppressing agonist dependent activation of several AR target genes we have tested , including PSA ( prostate specific antigen ) , TSC 22 ( TSC 22 domain family member 1 ) , NKX 3 1 ( NK 3 transcription factor locus 1 ) , and B2M ( beta 2 microglobulin ) . ^^^ Consistent with a role in both antagonist and agonist regulated transcription by AR , chromatin immunoprecipitation analysis revealed that both SMRT and N CoR were recruited by AR to these genes in the presence of either flutamide or R 1881 . ^^^ Knocking down SMRT and N CoR enhanced the recruitment of the coactivators steroid receptor coactivator 1 and p 300 by agonist bound AR and led to increased hyperacetylation of histone H 3 and H 4 , suggesting that the corepressors actively compete with coactivators for binding to agonist bound AR . ^^^ Taken together , our data indicate that SMRT and N CoR corepressors are involved in transcriptional regulation by both agonist and antagonist bound AR and regulate the magnitude of hormone response , at least in part , by competing with coactivators . . ^^^ |
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