| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| SMRT is highly related to another corepressor , N CoR , suggesting the existence of a new family of receptor interacting proteins . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Similarly , co repressors , such as SMRT and N CoR , for TR and retinoic acid receptors ( RAR ) have been identified . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| In concordance with this EcR interacts with the corepressors SMRT and N CoR , while addition of ligand reduces this interaction . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Regulation of SMRT and N CoR corepressor function . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The corepressors N CoR and SMRT partner with histone deacetylases ( HDACs ) in diverse repression pathways . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Early studies have suggested that HDAC 3 activity is regulated by association with the corepressors N CoR and SMRT . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| This short review will focus on N CoR and SMRT . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Similarly SMRT but not NCoR 1 gene transcription was sensitive to 1alpha , 25 ( OH ) ( 2 ) D ( 3 ) treatment . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We have isolated a new potential variant of RIP13 / N CoR that is missing previously described transcriptional repressor domains but is similar in structure to the related corepressor termed SMRT or TRAC 2 . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We report here that RTH receptors interact aberrantly with a newly recognized family of transcriptional corepressors variously denoted as nuclear receptor corepressor ( N CoR ) , retinoid 10 receptor interacting protein 13 ( RIP 13 ) , silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) , and thyroid hormone receptor associating cofactor ( TRAC ) . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| First , we have determined that receptor stoichiometry is a crucial determinant of transcriptional repression mediated by the corepressors N CoR and SMRT . ^^^ Thus , although N CoR and SMRT are capable of binding to several NHRs in solution , they are highly selective about receptor binding on DNA , a context that reflects their in vivo function more accurately . ^^^ These stoichiometric and steric principles govern specific interactions between corepressors and NHRs , thus providing evidence that N CoR and SMRT do not serve redundant functions but rather contribute to receptor specific transcriptional repression . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The transcriptional corepressors SMRT and N CoR function as silencing mediators for retinoid and thyroid hormone receptors . ^^^ Here we show that SMRT and N CoR directly interact with mSin3A , a corepressor for the Mad Max heterodimer and a homolog of the yeast global transcriptional repressor Sin3p . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Transcriptional repression by the thyroid hormone and retinoic acid receptors has been proposed to be mediated by the nuclear receptor corepressor , N CoR , or the related factor , SMRT ( silencing mediator of retinoic acid and thyroid hormone receptors ) . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The partial agonist activity of antagonist occupied steroid receptors is controlled by a novel hinge domain binding coactivator L7 / SPA and the corepressors N CoR or SMRT . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Gene silencing by chicken ovalbumin upstream promoter transcription factor 1 ( COUP TFI ) is mediated by transcriptional corepressors , nuclear receptor corepressor ( N CoR ) and silencing mediator for retinoic acid receptor and thyroid hormone receptor ( SMRT ) . ^^^ To determine which corepressor is involved in the COUP TF silencing activity , we used a yeast two hybrid and in vitro GST pull down assays to demonstrate that COUP TFI can interact with the fragment of N CoR ( nuclear receptor corepressor ) encoding amino acids 921 2453 and the fragments of SMRT ( silencing mediator for retinoic acid receptor and TR ) encoding amino acids 29 564 and 565 1289 , respectively . ^^^ Furthermore , overexpression of N CoR or SMRT potentiates the silencing activity of COUP TFI and can relieve the COUP TFI mediated squelching of Gal 4 COUP TFI activity . ^^^ Therefore , our studies indicate that N CoR and SMRT act as corepressors for the COUP TFI silencing activity . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Furthermore , we created functional silencer fusions which lose their repressive function upon addition of hormone , although the corepressors SMRT and N CoR remain attached to the receptor . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Normal RARs repress gene transcription by associating with ancillary factors denoted corepressors ( also referred to as SMRT , N CoR , TRAC , or RIP 13 ) . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The abilities of RORalpha polypeptides to repress transcription correlate with their abilities to interact with the nuclear receptor corepressors N CoR and SMRT in vitro . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We have found that both the TR CoR box and ninth heptad are required for RXR interaction and in turn for interaction with corepressor proteins N CoR and SMRT . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We describe here the cloning of a nuclear receptor corepressor that we call SUN CoR ( Small Unique Nuclear receptor CoRepressor ) , which shows no homology to previously described nuclear hormone receptor corepressors , N CoR , or SMRT . ^^^ SUN CoR also interacts with N CoR and SMRT in vitro and with endogenous N CoR in cells . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| SMRT ( silencing mediator of retinoic acid and thyroid hormone receptor ) and N CoR ( nuclear receptor corepressor ) are two related transcriptional corepressors that contain separable domains capable of interacting with unliganded nuclear receptors and repressing basal transcription . ^^^ SMRT ( silencing mediator of retinoic acid and thyroid hormone receptor ) and N CoR ( nuclear receptor corepressor ) are two related transcriptional corepressors that contain separable domains capable of interacting with unliganded nuclear receptors and repressing basal transcription . ^^^ To decipher the mechanisms of receptor interaction and transcriptional repression by SMRT / N CoR , we have characterized protein protein interacting surfaces between SMRT and nuclear receptors and defined transcriptional repression domains of both SMRT and N CoR . ^^^ Functional analysis reveals that SMRT contains two distinct repression domains , and the corresponding regions in N CoR also repress basal transcription . ^^^ Both repression domains in SMRT and N CoR interact weakly with mSin3A , which in turn associates with a histone deacetylase HDAC 1 in a mammalian two hybrid assay . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Non liganded retinoic acid receptors ( RARs ) repress transcription of target genes by recruiting the histone deacetylase complex through a class of silencing mediators termed SMRT or N CoR . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Diverse signaling pathways modulate nuclear receptor recruitment of N CoR and SMRT complexes . ^^^ Functional interactions between specific receptors and N CoR or SMRT corepressor complexes are regulated , positively or negatively , by diverse signal transduction pathways . ^^^ Our data suggest that N CoR and SMRT containing complexes act as rate limiting components in the actions of specific nuclear receptors , and that their actions are regulated by multiple signal transduction pathways . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that the inhibitory effect of AF2 / tau c in CV 1 cells can be overcome by expression of the corepressor SMRT ( silencing mediator of retinoic acid and thyroid hormone receptor ) , but not by that of N CoR ( nuclear receptor corepressor ) . ^^^ Cell specific inhibition of retinoic acid receptor alpha silencing by the AF2 / tau c activation domain can be overcome by the corepressor SMRT , but not by N CoR . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We examined and compared the expression of four coactivator ( steroid receptor coactivator 1 and E1A associated 300 kDa protein ) and corepressor ( SMRT and N CoR ) genes in a number of tissues including several endocrine glands and cell lines . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Finally , the interaction between DAX 1 and N CoR shares similarities with that of the nuclear receptor RevErb and N CoR , because the related corepressor SMRT was not efficiently recruited by DAX 1 . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Rev erbAalpha / beta ) , Mxi 1 and Mad bHLH zip proteins and the oncoproteins PLZF and LAZ3 / BCL6 is mediated by the corepressors N CoR and SMRT . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| In the absence of hormone , unliganded receptors interact with a family of transcriptional corepressors , including SMRT and N CoR , which target histone deacetylases to establish a condensed and repressed chromatin structure . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The ability to repress is closely linked to the ability of the apo receptor to physically bind to auxiliary corepressor proteins denoted SMRT ( silencing mediator of retinoic acid and thyroid hormone receptor ) and N CoR ( nuclear receptor corepressor ) , which , in turn , help mediate the actual molecular events involved in transcriptional silencing . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Repression appears to require a physical interaction between a receptor and a corepressor complex containing the SMRT / TRAC or N CoR / RIP13 polypeptides . ^^^ We report here that different receptors interact with different domains in the SMRT and N CoR corepressors and that these divergent interactions may therefore contribute to distinct repression phenotypes . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| For instance , the bHLH Zip transcriptional repressors MAD / MXI recruit HDACs together with the mSIN 3 corepressors , whereas unliganded nuclear receptors contact another corepressor , SMRT ( or its relative N CoR ) , which , in turn , associates with both mSIN 3 and HDACs to form the repressor complex . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We report here that ETO ( eight twenty one or MTG 8 ) , which is fused to the acute myelogenous leukemia 1 ( AML 1 ) transcription factor in t ( 8 ; 21 ) AML , interacts via its zinc finger region with a conserved domain of the corepressors N CoR and SMRT and recruits HDAC in vivo . ^^^ The fusion protein AML 1 ETO retains the ability of ETO to form stable complexes with N CoR / SMRT and HDAC . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The BCL 6 POZ domain and other POZ domains interact with the co repressors N CoR and SMRT . ^^^ Using a yeast two hybrid screen , we identified N CoR and SMRT as BCL 6 interacting proteins . ^^^ Both N CoR and SMRT , which were originally identified as co repressors for the unliganded nuclear thyroid hormone and retinoic acid receptors , are components of large complexes containing histone deacetylases . ^^^ Furthermore , when BCL 6 is bound to its consensus recognition sequence in vivo , it can interact with N CoR and SMRT . ^^^ We find , in vitro , that POZ domains from a variety of other POZ domain containing proteins , including the transcriptional repressor PLZF , as well as ZID , GAGA and a vaccinia virus protein , SalF17R , also interact with varying affinities with N CoR and SMRT . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| COUP TF interacts with the corepressors N CoR , SMRT and RIP 13 , and silences transcription by active repression and trans repression . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Transcriptional repression by COUP TFI is mediated by corepressors , N CoR ( nuclear receptor corepressor ) and SMRT ( silencing mediator for retinoid and thyroid hormone receptor ) , whereas transcriptional activation by SF 1 is mediated by coactivator SRC 1 ( steroid receptor coactivator 1 ) . ^^^ We therefore examined the expression of COUP TFI , SF 1 , SRC 1 , N CoR , and SMRT in a variety of adrenocortical adenomas and compared the results with CYP 17 mRNA levels . ^^^ Interestingly , the pattern of COUP TFI expression was similar to the profile of N CoR expression , but not of SMRT expression . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We have shown that transcriptional repression by COUP TFI is mediated by corepressors , N CoR ( nuclear receptor corepressor ) and SMRT ( silencing mediator for retinoid and thyroid hormone receptor ) . ^^^ Therefore , we compared the expression of COUP TFI , N CoR and SMRT in non hyperfunctioning adrenocortical adenomas and normal adrenal glands . ^^^ Interestingly , the pattern of N CoR and SMRT expression was different compared with COUP TFI expression . ^^^ These data suggest that COUP TFI , N CoR , and SMRT may play a differential role in steroid biosynthesis of non hyperfunctioning adenomas . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| This chimeric protein has been strongly implicated in APL pathogenesis because of its interactions with growth suppressors ( PML ) , retinoid signaling molecules ( RXRalpha ) , and nuclear hormone transcriptional co repressors ( N CoR and SMRT ) . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Studies by several groups demonstrate that this complex is recruited to nuclear receptors through the highly related corepressors SMRT ( silencing mediator of retinoid acid and thyroid hormone receptor ) and N CoR ( nuclear receptor corepressor ) . ^^^ We describe here the cloning , characterization , and chromosomal mapping of forms of human and mouse SMRT that includes a 1 , 000 aa extension , which reveals striking homology to the amino terminus of N CoR . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| SMRT ( silencing mediator for retinoid and thyroid hormone receptors ) and N CoR ( nuclear receptor copressor ) mediate transcriptional repression of important regulators that are involved in many signaling pathways . ^^^ SMRT and N CoR are related proteins that form complexes with mSin3A / B and histone deacetylases to induce local chromatin condensation and transcriptional repression . ^^^ However , SMRT is substantially smaller than N CoR , lacking an N terminal domain of approximately 1 , 000 aa that are present in N CoR . ^^^ Here , we report the identification of SMRT extended ( SMRTe ) , which contains an N terminal sequence that shows striking similarity with N CoR . ^^^ As in N CoR , this SMRTe N terminal domain also represses basal transcription . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Rev erbAalpha / beta ) , Mad / Max bHLH ( basic helix loop helix ) LZ proteins , and oncoproteins , PLZF and LAZ3 / BCL6 , bind DNA and silence transcription by recruiting a repressor complex that contains N CoR ( nuclear receptor corepressor ) / SMRT ( silencing mediator of retinoic acid and thyroid hormone receptor ) , Sin3A / B , and HDAc 1 / 2 proteins . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| In in vivo transcription assays we show that L7 / SPA enhances the partial agonist activity of type 2 mixed antagonists , and that N CoR and the related corepressor , SMRT , suppresses it . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Here , we report the isolation of an EcR interacting protein , SMRTER , which is structurally divergent but functionally similar to the vertebrate nuclear corepressors SMRT and N CoR . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| N CoR and SMRT are transcriptional corepressors that associate with nuclear hormone receptors ( NRs ) in the absence of ligand . ^^^ Here we show that N CoR and SMRT contain sequences that are similar to the NR box and are repeated in each of two NR interaction domains . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Recruitment of SMRT / N CoR mSin3A HDAC repressing complexes is not a general mechanism for BTB / POZ transcriptional repressors : the case of HIC 1 and gammaFBP B . ^^^ In the human B cell lymphoma ( BCL 6 ) and promyelocityc leukemia ( PLZF ) oncoproteins , this domain mediates transcriptional repression through its ability to recruit a silencing mediator of retinoid and thyroid hormone receptor ( SMRT ) / nuclear receptor corepressor ( N CoR ) mSin3A histone deacetylase ( HDAC ) complex , a mechanism shared with numerous transcription factors . ^^^ However , in striking contrast with BCL 6 and PLZF , both HIC 1 and gammaFBP B similarly fail to interact with members of the HDAC complexes ( SMRT / N CoR , mSin3A or HDAC 1 ) in vivo and in vitro . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We showed that for SRC 1 , CBP , TIF1alpha , RIP 140 , N CoR , and SMRT , no significant differences in the expression levels were observed between breast and endometrial cells . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Retinoic acid and thyroid hormone receptors can act alternatively as ligand independent repressors or ligand dependent activators , based on an exchange of N CoR or SMRT containing corepressor complexes for coactivator complexes in response to ligands . ^^^ The N CoR and SMRT nuclear receptor interaction motifs exhibit a consensus sequence of LXX I / H 1 30 I / L , representing an extended helix compared to the coactivator LXXLL helix , which is able to interact with specific residues in the same receptor pocket required for coactivator binding . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The association of transcription corepressors SMRT and N CoR with retinoid and thyroid receptors results in suppression of basal transcriptional activity . ^^^ We report here the identity of complementary acting signature motifs in SMRT and N CoR that are sufficient for receptor binding and ligand induced release . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Transcriptional repression mediated by corepressors N CoR and SMRT is a critical function of nuclear hormone receptors , and is dysregulated in human myeloid leukemias . ^^^ Surprisingly , however , numerous biochemical studies have not detected N CoR or SMRT in mSin 3 and HDAC 1 containing complexes . ^^^ Here we show that these RDs are nonredundant , and that one RD , which is conserved in N CoR and SMRT , represses transcription by interacting directly with class 2 HDAC 4 and HDAC 5 . ^^^ Endogenous N CoR and SMRT each associate with HDAC 4 in a complex that does not contain mSin3A or HDAC 1 . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Nuclear receptor corepressor ( N CoR ) and SMRT ( silencing mediator of retinoic acid and thyroid hormone receptor ) are corepressor proteins whose modular structure facilitates receptor interaction as well as transduction of repression signals involving histone deacetylation , alterations in chromatin structure and direct interactions with the basal transcription machinery . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate , using protein interaction assays in vitro and in vivo , that the inactive 5 ErbA point mutant L489R within helix 5 / 6 in SSD 2 fails to interact with the two corepressors N CoR ( nuclear receptor corepressor ) or SMRT ( silencing mediator of retinoic acid and thyroid hormone receptor ) . ^^^ In mammalian two hybrid assays , only the combination of all three silencing subdomains , SSD 1 3 , leads to a cooperative binding to the corepressors N CoR or SMRT comparable to that of the full length 5 ErbA repression domain . ^^^ Among these , SSD 2 is a new target for N CoR and SMRT and is essential for corepressor binding and function . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The resulting AML 1 ETO fusion is an aberrant transcriptional regulator due to the ability of ETO , which does not bind DNA itself , to recruit the transcriptional corepressors N CoR , SMRT , and Sin3A and histone deacetylases . ^^^ The N terminal portion of ETO forms complexes with PLZF , while the C terminal region , which was shown to bind to N CoR and SMRT , is required for the ability of ETO to augment transcriptional repression by PLZF . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| To obtain some clue to these roles , we screened the expression levels of ER alpha , ER beta , coactivators ( SRC 1 , TIF 2 , AIB 1 , CBP , and P / CAF ) and corepressors ( N CoR and SMRT ) in 6 normal mammary glands , 6 intraductal carcinomas , 22 invasive ductal carcinomas , and 7 breast cancer cell lines using a multiplex reverse transcription PCR . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| To examine this hypothesis , we cloned rat complementary DNA fragments corresponding to coactivators ( SRC 1 , TIF 2 and TRAM 1 ) and corepressors ( N CoR and SMRT ) , and studied the ontogenic changes in their corresponding messenger RNAs in rat cerebellum of normal and hypothyroid rats during postnatal development , using a RNase protection assay . ^^^ We found an increased expression of SRC 1 and TIF 2 , as well as of N CoR , during rat cerebellar development but no change in the expression of SMRT and TRAM 1 genes . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The POZ domains of BCL 6 and several other POZ proteins interact with corepressors N CoR and SMRT . ^^^ Additionally , interactions between the BCL 6 POZ domain and SMRT , N CoR , and BCoR are mutually exclusive . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Mammalian two hybrid analyses showed that RTR interacts with the co repressor nuclear co repressor ( N CoR ) but is unable to interact with the co repressor SMRT or RIP 140 . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Both corepressor proteins SMRT and N CoR exist in large protein complexes containing HDAC 3 . ^^^ We present evidence that both corepressors SMRT and N CoR exist in large protein complexes with estimated sizes of 1 . 5 2 MDa in HeLa nuclear extracts . ^^^ We show that the HDAC 3 containing SMRT and N CoR complexes can bind to unliganded thyroid hormone receptors ( TRs ) in vitro . ^^^ We demonstrate further that in XENOPUS : oocytes , both SMRT and N CoR also associate with HDAC 3 in large protein complexes and that injection of antibodies against HDAC 3 or SMRT / N CoR led to a partial relief of repression by unliganded TR / RXR . ^^^ These findings thus establish both SMRT and N CoR complexes as bona fide HDAC containing complexes and shed new light on the molecular pathways by which N CoR and SMRT function in transcriptional repression . . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| T3R mediated repression has been shown to involve co repressors such as the silencing mediator for retinoic acid and thyroid hormone receptor ( SMRT ) , N CoR or Alien . ^^^ At least one of these domains , the zinc finger region of CTCF , binds Sin3A without binding to SMRT or N CoR and recruits histone deacetylation activity . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The structure appears to contain both class 1 and the recently described class 2 histone deacetylases ( HDAC ) 5 and 7 along with the nuclear receptor corepressors SMRT ( silencing mediator for retinoid and thyroid receptor ) and N CoR ( nuclear receptor corepressor ) . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We have isolated two multiprotein N CoR complexes , designated N CoR 1 and N CoR 2 , which possess histone deacetylase activity that is mediated by distinct HDACs . ^^^ In contrast , N CoR 2 contained predominantly HDAC 1 and HDAC 2 as well as several other subunits that are found in the Sin3A . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Transcriptional repression plays crucial roles in diverse aspects of metazoan development , implying critical regulatory roles for corepressors such as N CoR and SMRT . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) and nuclear receptor co repressor ( N CoR ) interact with unliganded TR and function as corepressor proteins . ^^^ In contrast , wild type TR alpha 1 , alpha F397X and alpha E395X showed similar abilities to bind to RXR and SMRT . beta E449X and alpha E395X , which have identical C terminal truncation , showed less ability to bind to N CoR than did wild type TR beta 1 and beta F451X and wild type TR alpha 1 and alpha F397X respectively . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| ETO interacts with nuclear receptor corepressors SMRT and N CoR , which recruit histone deacetylase to the AML 1 ETO oncoprotein . ^^^ SMRT N CoR interaction requires each of two zinc fingers contained in C terminal Nervy homology region 4 ( NHR 4 ) of ETO . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We provide evidence that the transcriptional corepressors SMRT and N CoR could serve as critical mediators of HDAC 7 activity by binding class 2 HDACs and HDAC 3 by two distinct repressor domains . ^^^ Different class 2 HDACs reside in the cell nucleus in stable and autonomous complexes with enzymatic activity , but the enzymatic activities associated with HDAC 7 and HDAC 4 rely on shared cofactors , including HDAC 3 and SMRT / N CoR . . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Nuclear receptor corepressor ( N CoR ) and silencing mediator of retinoid and thyroid hormone receptors ( SMRT ) form heterogeneous complexes with various histone deacetylases ( HDACs ) . ^^^ These results suggest that ER alpha Delta AF 2 and similar mutant receptors recently found associated with certain tumors may actively perturb the normal E ( 2 ) signaling via SWI / SNF , N CoR / SMRT , and HDAC . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The SMRT and N CoR corepressors are activating cofactors for histone deacetylase 3 . ^^^ Thus , SMRT and N CoR do not serve merely as platforms for HDAC recruitment but function as an integral component of an active cellular HDAC 3 enzyme . . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Several lines of evidence have indicated that tamoxifen promotes association between ERalpha and corepressors N CoR or silencing mediator for retinoid and thyroid hormone receptor ( SMRT ) . ^^^ Our results indicate that N CoR / SMRT recognize and interact with helices H 3 and H 5 of the ERalpha ligand binding domain in a 4 hydroxy tamoxifen dependent manner . ^^^ The mutant ERalpha ( D351Y ) , derived from a tamoxifen stimulated tumor and containing an amino acid substitution at position 351 within H 3 , showed reduced interaction with N CoR / SMRT and high tamoxifen induced activation function 1 ( AF 1 ) activity . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| GCNF ' s repression function has been shown to be mediated by interaction with the co repressors N CoR and SMRT in the absence of ligand . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We find that two independent regions of Hr mediate TR binding and that interaction requires a cluster of hydrophobic residues similar to the binding motifs proposed for nuclear receptor corepressors ( N CoR and SMRT ) . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| On the other hand , antiestrogen bound receptors favour the assembly of receptor corepressor complexes containing the sequence related corepressors N CoR ( nuclear receptor corepressor ) or SMRT ( silencing mediator of retinoid and thyroid hormone receptors ) , localizing histone deacetylase activity to the promoter and leading to transcriptional repression . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Repression of gene transcription by nuclear receptors is mediated by interactions with co repressor proteins such as SMRT and N CoR , which in turn recruit histone deacetylases to the chromatin . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The closely related co repressor proteins N CoR and SMRT , although originally identified on the basis of their ability to associate with and confer transcriptional repression through nuclear receptors , have been shown to be recruited to many classes of transcription factor and are in fact components of multiple protein complexes containing histone deacetylase proteins . ^^^ This association with histone deacetylase activity provides an important component of the mechanism that allows DNA binding proteins interacting with N CoR or SMRT to repress transcription of specific target genes . ^^^ Both N CoR and SMRT are important targets for cell signaling pathways , which influence their expression levels , subcellular localization and association with other proteins . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We found that mutations of the BTB domain that neutralize key charged pocket residues did not disrupt dimerization , yet abrogated the ability of PLZF to repress transcription and led to the loss of interaction with N CoR , SMRT , and histone deacetylases ( HDACs ) . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Notably , tamoxifen induced association of ER with the transcriptional corepressors N CoR or SMRT was reduced in HER 2 overexpressing breast tumor cells but not in cells with low HER 2 levels . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Lastly , PRP4K , BRG 1 , and PRP 6 can be purified as components of the N CoR 2 complex , and affinity purified PRP4K / N CoR complexes exhibit deacetylase activity . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We therefore endeavoured to evaluate the presence of the co activator SRC ( steroid receptor co activator ) 1 and the co repressors N CoR ( nuclear receptor co repressor ) and steroid co repressor SMRT ( silencing mediator of retinod and thyroid ) receptors in the human endometrium during the different phases of the menstrual cycle . ^^^ By using a real time RT PCR assay , we showed that SRC 1 , N CoR and SMRT mRNA are expressed in human endometrium during all phases of the menstrual cycle , as well as in inactive endometrium . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| From a positive hormone response element ( pHRE ) and in the absence of hormone , corepressors SMRT and N CoR are bound to some nuclear receptors such as the thyroid hormone ( T3Rs ) and retinoic acid receptors and mediate inhibition of basal levels of transcription . ^^^ Here we show that corepressor SMRT can act as a potent coactivator for T3Ralpha from a nHRE ; N CoR has a similar but significantly attenuated activity . ^^^ Mutagenesis of residues in the hinge region of T3Ralpha that block binding of SMRT and N CoR inhibits ligand independent transcriptional activation by T3Ralpha from a nHRE . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Now , we show that cytoplasmic sequestration of p 65 by IkappaBalpha is sufficient to both translocate nuclear corepressors SMRT / N CoR to the cytoplasm and upregulate transcription of Notch dependent genes . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| N CoR and the highly related SMRT were originally isolated and characterized by their ability to interact exclusivelywith the unliganded forms of NHRs and confer transcriptional repression . ^^^ Recently , both the N CoR and SMRT corepressors have been found to exist in vivo in multiple , distinct macromolecular complexes . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Corepressors N CoR and SMRT participate in diverse repression pathways and exist in large protein complexes including HDAC 3 , TBL 1 and TBLR 1 . ^^^ However , the roles of these proteins in SMRT N CoR complex function are largely unknown . ^^^ Together , our data reveal the roles of HDAC 3 and TBL / TBLR1 and provide evidence for the functional importance of histone interaction in repression mediated by SMRT N CoR complexes . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| In the absence of the RAR specific ligand all trans retinoic acid , RAR / RXR heterodimers are associated with the nuclear receptor corepressor N CoR or the related SMRT . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Here we show that LXRs interact with corepressors , N CoR ( nuclear receptor corepressor ) and SMRT ( silent mediator of retinoic acid receptor and thyroid receptor ) , which are released upon binding agonists . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Nuclear receptor corepressors SMRT ( silencing mediator of retinoid and thyroid receptors ) and N CoR ( nuclear receptor corepressor ) recruit histone deacetylase ( HDAC ) activity to targeted regions of chromatin . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Nuclear receptor corepressors ( N CoR ) and silencing mediator for retinoid and thyroid receptors ( SMRT ) have both been implicated in thyroid hormone receptor ( TR ) mediated repression . ^^^ Knockdown of N CoR using small interfering RNAs markedly reduces repression by the TR ligand binding domain in human 293T cells , whereas knockdown of SMRT has little effect . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| It attaches to the nuclear matrix and associates with histone deacetylases and the co repressors N CoR , SMRT , and mSin3A , and may act as a co repressor for site specific transcriptions factors . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Furthermore , reinitiation of cell cycle progression by insulin / insulin like growth factor 1 in hydroxytamoxifen arrested cells involves dissociation of the corepressors nuclear receptor corepressor ( N CoR ) and silencing mediator for retinoid and thyroid hormone receptor ( SMRT ) from nuclear estrogen receptor alpha and redistribution to the cytoplasm , a process that is inhibited by mitogen activated protein / extracellular signal regulated kinase , but not phosphatidylinositol 3 ' kinase , inhibitors . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The homodimeric BTB domain of BCL 6 ( also known as the POZ domain ) is required for the repression activity of the protein and interacts directly with the SMRT and N CoR corepressors that are found within large multiprotein histone deacetylase containing complexes . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| METHODS : Gene expression of SRC 1 , SRC 2 , SRC 3 , N CoR , SMRT , ERalpha , and PR was measured in 26 samples of normal endometrium and 30 primary endometrial carcinomas using real time RT PCR . ^^^ CONCLUSION : The nuclear receptor coregulators SRC 1 , SRC 2 , SRC 3 , N CoR , and SMRT were found to be up regulated in malignant endometrium . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| TBL1 / TBLR1 serve as specific adaptors for the recruitment of the ubiquitin conjugating / 19S proteasome complex , with TBLR 1 selectively serving to mediate a required exchange of the nuclear receptor corepressors , N CoR and SMRT , for coactivators upon ligand binding . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| METHODS : Multiprobe ribonuclease protection assay and real time reverse transcriptase polymerase chain reaction were used to quantitate mRNA levels of steroid receptors , vitamin D receptor ( VDR ) , retinoic acid receptors ( RAR ) , and cofactors AIB 1 ( amplified in breast cancer 1 ) , CBP ( cyclic adenosine monophosphate response element binding protein ) , pCAF ( p300 / CBP associated factor ) , TIF 2 ( transcription intermediary factor 2 ) , N CoR ( nuclear receptor corepressor ) , and SMRT ( silencing mediator of repressed transcription ) . ^^^ Cofactors N CoR , SMRT , pCAF , CBP , TIF 2 , AIB 1 , and p 300 mRNAs were expressed in all samples in both endometrium and myometrium . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Two repressors of ligand dependent transcription factors , nuclear receptor corepressor ( N CoR ) and the related protein SMRT were identified as a silencing mediator for thyroid hormone receptor beta and as a silencing mediator for retinoic acid and thyroid hormone receptors , respectively . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| SMRT and N CoR corepressors are regulated by distinct kinase signaling pathways . ^^^ N CoR and SMRT are corepressor paralogs that partner with and mediate transcriptional repression by a wide variety of metazoan transcription factors , including nuclear hormone receptors . ^^^ Although encoded by distinct genetic loci , N CoR and SMRT share substantial sequence interrelatedness , form analogous assemblies with histone deacetylases and auxiliary factors , can interact with overlapping sets of transcription factor partners , and exert overlapping functions in cells . ^^^ We report here that whereas activation of MEKK 1 leads to phosphorylation of SMRT , its dissociation from its transcription factor partners in vivo and in vitro , and its redistribution from the cell nucleus to a cytoplasmic compartment , N CoR is refractory to all these forms of regulation . ^^^ Our results indicate that SMRT and N CoR are embedded in distinct regulatory networks and that the two corepressors interpret growth factor , cytokine , differentiation , and prosurvival signals differently . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| On the other hand , although overexpression of corepressors N CoR and SMRT could result in evident inhibition on DHT or CPA induced transactivity of wtAR and the AR mutants , N CoR displayed stronger inhibitory effects on DHT induced transactivity of the AR mutants ( especially for E872Q and M886I ) than that of wtAR . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| They interact with corepressors , such as N CoR and SMRT , that recruit a multiprotein complex containing histone deacetylases on crucial myeloid differentiation genes . ^^^ This blocks N CoR / SMRT binding by these fusion proteins , and disrupts the repressor protein complex . ^^^ The alteration of PML / RARalpha N CoR / SMRT connections triggers proteasomal degradation of the fusion protein . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Cell cycle progression stimulated by tamoxifen bound estrogen receptor alpha and promoter specific effects in breast cancer cells deficient in N CoR and SMRT . ^^^ Tamoxifen bound ERalpha associates with nuclear receptor corepressor ( N CoR ) and silencing mediator for retinoid and thyroid hormone receptors ( SMRT ) at certain target genes . ^^^ Here we show the effects of reducing N CoR and SMRT levels on the actions of estrogen and tamoxifen in breast cancer cells . ^^^ By contrast , expression of 10 box binding protein 1 was markedly elevated in tamoxifen treated cells in which N CoR and SMRT had been silenced . ^^^ The gain in cell cycle entry seen with tamoxifen when N CoR and SMRT were silenced was dependent on ERalpha and not observed upon treatment with estradiol or epidermal growth factor . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Unliganded thyroid hormone ( TH ) receptors ( TRs ) and other nuclear receptors ( NRs ) repress transcription of hormone activated genes by recruiting corepressors ( CoRs ) , such as NR CoR ( N CoR ) and SMRT . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The aim of the study was to test the hypothesis that expression of retinoid receptors ( RARalpha , RARbeta , RARgamma ) , rexinoid receptors ( RXRalpha , RXRbeta ) , thyroid hormone receptors ( TRalpha , TRbeta ) , estrogen receptors ( ERalpha , ERbeta ) , nuclear receptor coregulators ( N CoR , SRC 1 , SMRT ) , and in addition type 1 iodothyronine 5 ' deiodinase ( 5 ' DI ) , EGFR and erb B2 / neu would be different in mammary postlactating tissue in comparison with that of nonlactating mammary gland . ^^^ Using RT PCR , we have shown that expression of RARalpha , RXRalpha , TRalpha , ERalpha , ERbeta , N CoR , SRC 1 , SMRT and EGFR in rat was significantly increased in postlactating mammary gland when compared to that of nonlactating mammary tissue . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Finally , we report that a C terminal truncation in 5 Erb A not only inhibits exchange of corepressor and coactivator , as previously noted , but also permits 5 Erb A to recruit both SMRT and N CoR corepressors , whereas c Erb A is selective for N CoR . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Corepressor N CoR ( nuclear receptor corepressor ) and the highly related protein SMRT ( silencing mediator of retinoid and thyroid hormone receptor ) play important roles in different biological processes including proliferation , differentiation , and development . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| By contrast , the retinoid receptors associated transcriptional co factors , including the co repressors of N CoR and SMRT , and the co activators of SRC 1 and P / CAF , were ubiquitously expressed in the developing telencephalon . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| In Saccharomyces cerevisiae , the SIF 2 gene product is an integral component of the Set 3 complex ( SET3C ) , an assembly of proteins with some homology to the human SMRT and N CoR corepressor complexes . ^^^ Since Sif2p appears to be the yeast homolog of human TBL 1 and TBLR 1 , which function in the N CoR / SMRT complexes , its structural and oligomeric properties are likely to be very similar . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We have found that estrogen markedly down regulates N CoR protein levels in estrogen receptor ( ER ) positive breast cancer cells without affecting N CoR mRNA levels , whereas levels of the related corepressor SMRT are unaffected . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Estrogen independent cells were still estrogen responsive and PR , nuclear receptor corepressor ( N CoR ) and SMRT expression was increased whereas steroid receptor coactivator 1 ( SRC 1a ) and CBP related protein p 300 ( p 300 ) expression decreased . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Neuroanatomical distribution and colocalisation of nuclear receptor corepressor ( N CoR ) and silencing mediator of retinoid and thyroid receptors ( SMRT ) in rat brain . ^^^ The two structurally related nuclear receptor corepressor ( N CoR ) and silencing mediator of retinoid and thyroid receptors ( SMRT ) proteins have been found to differentially affect the transcriptional activity of numerous nuclear receptors , such as thyroid hormone , retinoic acid and steroid receptors . ^^^ We find that although both N CoR and SMRT transcripts are ubiquitously expressed in brain , striking differences in their respective levels of expression could be observed in discrete areas of brain stem , thalamus , hypothalamus and hippocampus . ^^^ Using dual label immunofluorescence , we examined in selected glucocorticoid sensitive areas involved in the regulation of the hypothalamus pituitary adrenal axis activity , the respective protein abundance of N CoR and SMRT . ^^^ Colocalisation of N CoR and SMRT was demonstrated by confocal microscopy in most areas studied . ^^^ |
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| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| We have investigated the role of corepressors SMRT ( silencing mediator of retinoid and thyroid hormone receptor ) and N CoR ( nuclear receptor corepressor ) in transcriptional regulation by androgen receptor ( AR ) in the LNCaP prostate cancer cell line . ^^^ Using specific small interference RNAs to knock down SMRT and / or N CoR in LNCaP cells , we found that SMRT and N CoR not only mediate antagonist dependent inhibition of AR activation but also have a widespread role in suppressing agonist dependent activation of several AR target genes we have tested , including PSA ( prostate specific antigen ) , TSC 22 ( TSC 22 domain family member 1 ) , NKX 3 1 ( NK 3 transcription factor locus 1 ) , and B2M ( beta 2 microglobulin ) . ^^^ Consistent with a role in both antagonist and agonist regulated transcription by AR , chromatin immunoprecipitation analysis revealed that both SMRT and N CoR were recruited by AR to these genes in the presence of either flutamide or R 1881 . ^^^ Knocking down SMRT and N CoR enhanced the recruitment of the coactivators steroid receptor coactivator 1 and p 300 by agonist bound AR and led to increased hyperacetylation of histone H 3 and H 4 , suggesting that the corepressors actively compete with coactivators for binding to agonist bound AR . ^^^ Taken together , our data indicate that SMRT and N CoR corepressors are involved in transcriptional regulation by both agonist and antagonist bound AR and regulate the magnitude of hormone response , at least in part , by competing with coactivators . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The relative affinity for the SMRT versus N CoR corepressors was detectably altered for several of the HCC mutant TRs , suggesting changes in corepressor preference and recruitment compared to wild type . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| A diverse cadre of metazoan transcription factors mediate repression by recruiting protein complexes containing the SMRT ( silencing mediator of retinoid and thyroid hormone receptor ) or N CoR ( nuclear receptor corepressor ) corepressors . ^^^ SMRT and N CoR nucleate the assembly of still larger corepressor complexes that perform the specific molecular incantations necessary to confer transcriptional repression . ^^^ Although SMRT and N CoR are paralogs and possess similar molecular architectures and mechanistic strategies , they nonetheless exhibit distinct molecular and biological properties . ^^^ It is now clear that the functions of both SMRT and N CoR are further diversified through alternative mRNA splicing , yielding a series of corepressor protein variants that participate in distinctive transcription factor partnerships and display distinguishable repression properties . ^^^ This review will discuss what is known about the structure and actions of SMRT , N CoR , and their splicing variants , and how alternative splicing may allow the functions of these corepressors to be adapted and tailored to different cells and to different developmental stages . . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The data suggest that the partial agonism of HA may be explained in part by effective displacement of corepressors ( N CoR and SMRT ) coupled with inefficient recruitment of coactivators ( p 300 , CBP , and TRAP 220 ) . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Moreover , NCOR 1 alone or together with NCOR 2 did not restrict the ability of the PyV ori to reinitiate replication within a single S phase and did not require any BPV protein to exert suppression . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The expression of 11 nuclear receptor co regulatory factors ( SRC 1 , AIB 1 , ARA 24 , ARA 54 , ARA 55 , ARA 70 , ARA 160 , FHL 2 , PDEF , NCoR 1 , SMRT ) was compared in these cell lines by semi quantitative RT PCR to determine if the pattern of receptor co activators or repressors expressed in these cells might explain the differential regulation of KLK 2 and KLK 3 . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Recombinant corepressors mSin3A , SMRT , and HDAC 1 , but not NCoR 1 , interacted with GST SHP but each of these corepressors in liver nuclear extracts bound to GST SHP . ^^^ SMRT and NCoR 1 inhibited CAR mediated activation independent of SHP , but mSin3A and HDAC 1 had little effect alone , and were additive with SHP . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| Similarly , 10 / 15 primary tumour cultures ( including three matched to normal cells from the same donors ) had elevated SMRT mRNA levels ; generally NCoR 1 and Alien were not as commonly elevated . ^^^ |
|
| Interacting proteins: O75376 and Q9Y618 |
Pubmed |
SVM Score :0.0 |
| The traC gene encodes two acidic and hydrophilic polypeptide chains of 1061 ( TraC 1 ) and 746 ( TraC 2 ) amino acids corresponding to molecular masses of 116 , 721 and 81 , 647 Da . ^^^ |
|