| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :1.1165643 |
| Rac likely facilitates the membrane localization of Noxa 1 : although Noxa 1 ( W436R ) , defective in Noxo 1 binding , neither associates with the membrane nor activates Nox 1 , the effects of the W436R substitution are restored by expression of Rac 1 ( Q61L ) . 1.1165643^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| In conclusion , NOX 1 is a superoxide generating enzyme that is activated by two novel proteins , which we propose to name NOXO 1 ( NOX organizer 1 ) and NOXA 1 ( NOX activator 1 ) . . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| Co transfection of Nox 1 , NOXO 1 , and NOXA 1 reconstitutes ROS ( reactive oxygen species ) generation in HEK 293 cells in the absence of cell stimulation . ^^^ Thus , in transfected HEK 293 cells , NOXO 1 and NOXA 1 activate Nox 1 without the need for agonist activation , and this is mediated in part by binding of the NOXO 1 PX domain to membrane lipids . . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| Nox 1 , a homolog of gp 91 ( phox ) , is regulated by NOXO 1 and NOXA 1 , homologs of p 47 ( phox ) and p 67 ( phox ) , respectively . ^^^ For both Nox 1 and gp 91 ( phox ) , an organizer protein ( NOXO 1 or p 47 ( phox ) ) cooperates with an activator protein ( NOXA 1 or p 67 ( phox ) ) to regulate the catalytic subunit . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| Studies of cytosolic co factors showed that the C terminal cytoplasmic domain of NOX 1 was absolutely required for activation with NOXO 1 and NOXA 1 and that this activity required interaction of the putative NADPH binding region of this domain with NOXA 1 . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| Activation of Nox 1 , an oxidase that is likely involved in host defence at the colon , requires novel proteins homologous to p47phox and p67phox , designated Noxo 1 and Noxa 1 , respectively . ^^^ Noxo 1 and Noxa 1 , both expressed abundantly in the colon , are capable of constitutively activating Nox 1 . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| The activity of other Nox enzymes such as gp 91 ( phox ) / Nox2 and Nox 1 is known to absolutely require both an organizer protein ( p 47 ( phox ) or Noxo 1 ) andanactivatorprotein ( p 67 ( phox ) or Noxa 1 ) ; for the p 47 ( phox ) dependent activation of these oxidases , treatment of cells with stimulants such as phorbol 12 myristate 13 acetate is also indispensable . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| Novel homologs of p47phox and p67phox ( NOXO 1 and NOXA 1 , respectively ) were recently identified and are implicated in the regulation of the gp91phox homologs Nox 1 and Nox 3 . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| For example , an oxidase activator ( p47phox or Noxo 1 ) and an oxidase activator ( p67phox or Noxa 1 ) are absolutely required for superoxide production by gp91phox and Nox 1 , but not by Nox 3 . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| Noxa 1 can support Nox 1 without Noxo 1 , when targeted to the plasma membrane by fusing membrane binding sequences from Rac 1 ( amino acids 183 to 192 ) to the C terminus of Noxa 1 . ^^^ However , membrane targeting of Noxa 1 is not sufficient for activation of Nox 1 . ^^^ Both the Noxo 1 independent and dependent Nox 1 systems involve Rac 1 , since they are affected by Rac 1 mutants or Noxa 1 mutants defective in Rac binding or short interfering RNA mediated Rac 1 silencing . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| When Nox 1 is co expressed along with its regulatory subunits NOXO 1 and NOXA 1 , significant ROS generation is seen . ^^^ TPR domain mutants of NOXA 1 that interfere with Rac 1 binding were ineffective in supporting Nox 1 dependent ROS generation . ^^^ Immunoprecipitation experiments demonstrated a complex containing Rac 1 ( G12V ) , NOXO 1 , NOXA 1 , and Nox 1 . ^^^ Nox 1 formed a complex with Rac 1 ( G12V ) that was independent of NOXA 1 and NOXO 1 , consistent with direct binding of Rac 1 ( G12V ) to Nox 1 . ^^^ Rac 1 ( G12V ) interaction with NOXA 1 was enhanced by Nox 1 and NOXO 1 , suggesting cooperative binding . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| Activation of the superoxide producing NADPH oxidase Nox 1 requires both the organizer protein Noxo 1 and the activator protein Noxa 1 . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| Nox 1 , a homologue of gp91phox , also can be activated in cells , when it is coexpressed with p41nox and p51nox , with p41nox and p67phox , or with p47phox and p51nox ; in the former two cases , Nox 1 is partially activated without any stimulants added , suggesting that p41nox is normally in an active state . ^^^ |
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| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q86UR1 and Q9Y5S8 |
Pubmed |
SVM Score :0.0 |
| NA |
|