| Body weight and composition , 24 h energy metabolism ( whole body indirect calorimetry ) , and maximal activities of phosphofructokinase ( EC 2 . 7 . 1 . 11 ) , creatine kinase ( CK ; EC 2 . 7 . 3 . 2 ) , citrate synthase ( CS ; EC 4 . 1 . 3 . 7 ) , 3 hydroxyacyl CoA dehydrogenase ( HADH ; EC 1 . 1 . 1 . 35 ) , and cytochrome c oxidase ( COX ; EC 1 . 9 . 3 . 1 ) were determined from biopsy samples of the vastus lateralis taken before and after weight loss . ^^^ |