| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| A beta TrCP mutant lacking the F box inhibits the signal induced degradation of IkappaBalpha and subsequent activation of NF kappaB dependent transcription . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Expression of a F box deleted betaTrCP inhibits IkappaBalpha degradation , promotes accumulation of phosphorylated Ser 32 Ser36 IkappaBalpha , and prevents NF kappaB dependent transcription . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Therefore , these results indicate that beta TrCP plays a critical role in the activation of NF kappaB by assembling the ubiquitin ligase complex for both phosphorylated IkappaBalpha and IkappaBbeta . . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Herein we reveal that deletion mutants of betaTrCP 1 and betaTrCP 2 lacking the F box domain suppressed ubiquitination and destruction of pIkappaBalpha as well as transcriptional activation of NF kappaB . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Beta transducing repeat containing protein ( betaTrCP ) targets the ubiquitination and subsequent degradation of both beta catenin and IkappaB , thereby playing an important role in beta catenin / Tcf and NF kappaB dependent signaling . ^^^ This signaling also upregulated NF kappaB transactivation without affecting the activity of IkappaB kinase , thereby establishing that the maintenance of the betaTrCP level is important for coordination between beta catenin / Tcf and NF kappaB signaling . . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Shared pathways of IkappaB kinase induced SCF ( betaTrCP ) mediated ubiquitination and degradation for the NF kappaB precursor p 105 and IkappaBalpha . p 105 ( NFKB 1 ) acts in a dual way as a cytoplasmic IkappaB molecule and as the source of the NF kappaB p 50 subunit upon processing . p 105 can form various heterodimers with other NF kappaB subunits , including its own processing product , p 50 , and these complexes are signal responsive . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of NF kappaB inhibitor ( IkappaB ) by respective stress inducible kinases ( IKK ) is a key event in NF kappaB activation . beta TrCP F box protein mediates ubiquitination of phosphorylated IkappaB via recruitment of SCF ( beta TrCP ) Roc 1 E3 ubiquitin ligase complex . ^^^ The mechanisms of beta TrCP induction via stress and its role in NF kappaB activation are discussed . . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Although the mechanisms that underlie processing of p 105 are largely obscure , it is clear that NEDDylation and the coordinated activity of SCF ( beta TrCP ) on both p 105 and IkappaBalpha serve as an important regulatory mechanism controlling NF kappaB activity . . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| The DNA region of the putative beta TrCP promoter contains several TATA elements , one GCCAAT box , and putative binding sites for Ets , Tcf / Lef and NF kappa B transcription factors . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : Upregulation of beta TrCP 1 accelerated the rate of IkappaBalpha degradation , leading to increased NF kappaB activity . ^^^ In contrast , VSMCs harboring a dominant negative beta TrCP 1 transgene lacking the F box domain exhibited a reduction in serum stimulated NF kB activity but no alteration in response to tumor necrosis factor ( TNF ) . ^^^ These findings suggest that beta TrCP 1 increases the rate of NF kappaB activation but is not rate limiting in response to TNF in VSMCs . ^^^ Upregulation of Wnt 1 resulted in beta catenin mediated activation of Tcf 4 , leading to increased beta TrCP 1 expression and NF kappaB activity . ^^^ Furthermore , VSMCs harboring a Tcf 4 mutant lacking a beta catenin binding domain exhibited a significant reduction in beta TrCP 1 expression along with abolishment of NF kappaB activity . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Associations among beta TrCP , an E 3 ubiquitin ligase receptor , beta catenin , and NF kappaB in colorectal cancer . ^^^ BACKGROUND : The ubiquitin proteasome pathway is important in regulating protein signaling pathways that are involved in tumorigenesis . beta transducin repeat containing proteins ( beta TrCP ) are components of the ubiquitin ligase complex targeting beta catenin and IkappaBalpha for proteasomal degradation and are thus a negative regulator of Wnt / beta catenin signaling and a positive regulator of NF kappaB signaling . ^^^ Increased beta TrCP 1 levels were statistically significantly associated with beta catenin activation ( P = . 023 ) and decreased apoptosis ( P = . 035 ) . beta TrCP accumulated in the nuclei of tumor cells that contained increased levels of beta TrCP 1 mRNA and the active form of NF kappaB . ^^^ CONCLUSION : In colorectal cancer , increased expression of beta TrCP 1 is associated with activation of both beta catenin and NF kappaB , suggesting that the integration of these signaling pathways by increased beta TrCP expression may contribute to an inhibition of apoptosis and tumor metastasis . . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| In contrast to the physiological processing of p 100 triggered by NF kappaB inducing kinase ( NIK ) and its downstream kinase , IkappaB kinase alpha ( IKKalpha ) , which requires the E 3 ligase , beta transducin repeat containing protein ( beta TrCP ) , and occurs only in the cytoplasm , the constitutive processing of p 100 is independent of beta TrCP but rather is regulated by the nuclear shuttling of p 100 . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Here , we identified that a phosphorylated form of IkappaBalpha ( pIkappaBalpha ) , an inhibitor of NF kappaB , and SCF ( beta TrCP ) , the ubiquitin ligase of pIkappaBalpha , are components of LB in brains of PD patients . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Canonical inducible NF kappaB activation involves IkappaB kinase ( IKK ) dependent dual phosphorylation of Ser 32 and 36 of IkappaBalpha to cause its beta TrCP dependent ubiquitylation and proteasomal degradation . ^^^ We recently reported that constitutive NF kappaB ( p50 / c Rel ) activity in WEHI 231 B cells is maintained through proteasome inhibitor resistant ( PIR ) IkappaBalpha degradation in a manner that requires Ser 32 and 36 , without the requirement of a direct interaction with beta TrCP . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| We present evidence that the effector OspG is a protein kinase that binds various ubiquitinylated ubiquitin conjugating enzymes , including UbcH 5 , which belongs to the stem cell factor SCF ( beta TrCP ) complex promoting ubiquitination of phosphorylated inhibitor of NF kappaB type alpha ( phospho IkappaBalpha ) . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| This work investigated the capacity of alpha lipoic acid ( LA ) and N acetyl L cysteine ( NAC ) to reduce zinc deficiency induced oxidative stress , and prevent the activation of nuclear factor kappaB ( NF kappaB ) and activator protein 1 ( AP 1 ) , and the cross talk between both activated cascades through beta Transducin Repeat containing Protein ( beta TrCP ) . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| In addition , we identified a phosphorylated form of IkappaBalpha ( pIkappaBalpha ) , an inhibitor of the NF kappaB signaling pathway , and the components of the SCF ( beta TrCP ) , ubiquitin ligase of pIkappaBalpha , are novel protein components in LBs . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| High levels of CRD BP that are found in primary human colorectal tumours exhibiting active beta catenin / Tcf signalling implicates CRD BP induction in the upregulation of betaTrCP 1 , in the activation of dimeric transcription factor NF kappaB and in the suppression of apoptosis in these cancers . . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| Impaired degradation of inhibitory subunit of NF kappa B ( 1 kappa B ) and beta catenin as a result of targeted disruption of the beta TrCP 1 gene . beta TrCP 1 ( also known as Fbw1a or FWD 1 ) is the F box protein component of an Skp1 / Cul1 / F box ( SCF ) type ubiquitin ligase complex . ^^^ Although biochemical studies have suggested that beta TrCP 1 targets inhibitory subunit of NF kappa B ( 1 kappa B ) proteins and beta catenin for ubiquitylation , the physiological role of beta TrCP 1 in mammals has remained unclear . ^^^ The nuclear translocation and DNA binding activity of NF kappa B as well as the ability of this transcription factor to activate a luciferase reporter gene were also inhibited in beta TrCP 1 / cells compared with those apparent in wild type cells . ^^^ These results suggest that beta TrCP 1 contributes to , but is not absolutely required for , the degradation of 1 kappa B and beta catenin and the consequent regulation of the NF kappa B and Wnt signaling pathways , respectively . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| The introduction of FWD 1 into cells significantly promotes ubiquitination and degradation of IkappaBalpha in concert with IkappaB kinases , resulting in nuclear translocation of NF kappaB . ^^^ Skp1 / Cullin / F box protein FWD 1 might play a critical role in transcriptional regulation of NF kappaB through control of IkappaB protein stability . . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| FWD 1 may thus play an important role in NF kappaB signal transduction through regulation of the stability of multiple IkappaB proteins . . ^^^ |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9Y297 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
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