| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Adhesion of phorbol ester activated LAD PMN to the extracellular matrix proteins fibronectin , laminin , and vitronectin failed to induce paxillin tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Focal adhesion complexes ( FACs ) containing integrin beta 1 , talin , vinculin , talin , alpha actinin , and paxillin formed within 15 min when round cells bound magnetic microbeads coated with integrin ligands , such as fibronectin or RGD containing peptide , but not when coated with acetylated low density lipoprotein . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Here we show that pp41 / 43FRNK acts as an inhibitor of pp125FAK by transiently blocking the formation of focal adhesions on fibronectin and constitutively reducing tyrosine phosphorylation of both pp125FAK and two focal adhesion proteins , tensin and paxillin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Immunofluorescence microscopy of cells grown on fibronectin confirmed the presence of paxillin , talin , and vinculin at the ends of actin stress fibers at presumptive focal contacts in melanocytes . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Our data indicated that , while talin , vinculin , paxillin , fibronectin receptor and integrin beta 1 were present at the three stress fiber membrane association sites , vitronectin receptor and integrin alpha 5 were absent from the apical plaque and the lateral association site . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| In this report , we have characterized the effect of stem cell factor on the organization of the actin cytoskeleton in human melanocytes attached to fibronectin , and have examined the effect of stem cell factor on the phosphorylation of the focal contact protein paxillin and on the expression of the focal contact proteins talin , paxillin , vinculin , and alpha actinin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation and immunoblotting with antiphosphotyrosine antibody showed that adherence of TF 1 cells to fibronectin without cytokine caused tyrosine phosphorylation of several proteins identified as pp125FAK and paxillin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| We found that vinculin , talin , paxillin , a fibronectin receptor protein , several integrin subunits including beta 1 , fibronectin , and proteins with phosphorylated tyrosine were also components of the apical plaque . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Adhesion of fibroblasts to fibronectin stimulates both serine and tyrosine phosphorylation of paxillin . ^^^ To assess the physiological relevance of these sites phosphorylated in vitro , the profile of paxillin phosphorylation in vivo stimulated by seeding fibroblasts on fibronectin was characterized . ^^^ As expected , plating cells on fibronectin enhanced the tyrosine phosphorylation of paxillin . ^^^ Comparison by two dimensional phosphopeptide analyses indicated that the major sites of tyrosine and serine phosphorylation detected in the assays in vitro co migrate with phosphopeptides derived from paxillin phosphorylated in vivo in response to plating cells on fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Most significantly , these cells expressed markedly higher levels of both intracellular and extracellular fibronectin , thus providing a mechanism for activation of FAK and increased tyrosine phosphorylation of paxillin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Pervanadate induced adhesion of CD4+ T cell to fibronectin is associated with tyrosine phosphorylation of paxillin . ^^^ We demonstrate that the cytoskeletal protein paxillin underwent time dependent tyrosine phosphorylation simultaneously with pervanadate induced T cell adhesion to fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Proline rich tyrosine kinase 2 activation by beta 1 integrin fibronectin receptor cross linking and association with paxillin in human natural killer cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| We analyzed the molecular basis for this change : bFGF modified the protein expression of alpha smooth muscle actin ( alpha SMA ) , tropomyosin , alpha tubulin , fibronectin and paxillin in a distinct manner characteristic of each of the stromal cell types . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| This report demonstrates that tyrosine phosphorylation of paxillin and FAK elicited by stimulation of muscarinic m 3 receptors with the acetylcholine analog carbachol is inhibited by soluble peptides containing the arginine glycine aspartate motif ( the recognition site for integrins found in adhesion proteins such as fibronectin ) but is unaffected by peptides containing the inactive sequence arginine glycine glutamate . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Serine and threonine phosphorylation of the paxillin LIM domains regulates paxillin focal adhesion localization and cell adhesion to fibronectin . ^^^ K1 cells to fibronectin ; consequently , a role for LIM domain phosphorylation in regulating the subcellular localization of paxillin after adhesion to fibronectin was investigated . ^^^ We found that mutations of paxillin that mimicked LIM domain phosphorylation accelerated fibronectin induced localization of paxillin to focal contacts . ^^^ The potentiation of FA targeting and cell adhesion to fibronectin was specific to LIM domain phosphorylation as mutation of the amino terminal tyrosine and serine residues of paxillin that are phosphorylated in response to fibronectin adhesion had no effect on the rate of FA localization or cell adhesion . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Furthermore , regulated association of Src SH 2 domain with FAK and paxillin during cell attachment and detachment on fibronectin was disrupted in Shp 2 mutant cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| The undifferentiated cells ( a ) were unable to spread on gelatin or laminin and grew as rounded colonies , although they were able to spread on fibronectin ( b ) showed reduced adhesion to laminin , but not fibronectin ( c ) expressed much reduced levels of beta 1 integrin , although levels of alpha 5 and alphaV were wild type ( d ) were less polarized with increased membrane protrusions compared with a vinculin ( / ) ES cell mutant ( e ) were unable to assemble vinculin or paxillin containing focal adhesions or actin stress fibers on fibronectin , whereas vinculin ( / ) ES cells were able to assemble talin containing focal adhesions . ^^^ Interestingly , these differentiated talin ( / ) ES cells were able to spread and form focal adhesion like structures containing vinculin and paxillin on fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Cytostatin inhibited tyrosine phosphorylation of focal adhesion kinase ( FAK ) and paxillin upon B 16 cell adhesion to fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Transformed cells growing on fibronectin pre coated dishes regain their flat morphology and this is accompanied by paxillin tyrosine phosphorylation . ^^^ Furthermore , immunoprecipitation analysis showed that paxillin forms a heteromeric complex with PLC gamma 1 in cells grown on fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Paxillin LIM zinc finger phosphorylation in response to cell adhesion regulates the subcellular localization of this cytoskeletal adaptor protein to focal adhesions , and also modulates cell adhesion to fibronectin ( Brown et al . [ 1998 ] Mol . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| The mutant exhibited reduced levels of phosphotyrosine relative to wild type paxillin in subconfluent cells growing in culture , following cell adhesion to fibronectin and in src transformed fibroblasts . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| However , clustering of beta1B did not induce tyrosine phosphorylation of FAK , p 130 ( Cas ) , or paxillin , as studied by beta1B mediated adhesion , to fibronectin in the presence of Mn ( 2+ ) or to anti beta 1 antibody in DMEM . ^^^ Stimulation of tyrosine phosphorylation on FAK , p 130 ( Cas ) , and paxillin by adhesion via integrin alphaVbeta 3 to fibronectin or vitronectin was not disturbed in GD 25 beta1B cells compared to the untransfected GD 25 cells , nor were any negative effects of beta1B observed on alphaVbeta 3 mediated cell attachment , spreading , and actin organization , or on the cell proliferation rate . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Attachment to fibronectin induces tyrosine phosphorylation of focal adhesion kinase ( FAK ) and paxillin in parental cells and cells transfected with the wild type PTP1B , while in cells transfected with the mutant PTP1B , such induction is not observed . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| On the other hand , overexpression of Csk DeltaK induced tyrosine phosphorylation of cellular proteins , including the paxillin and focal adhesion kinase ( FAK ) and enhanced to some extent the cytoskeletal organization and the rate of cell spreading on fibronectin , indicating that Src or its relatives was functionally activated in the cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Focal adhesion kinase , p 190 Rho GAP , tensin , and paxillin were tyrosine phosphorylated in response to soluble fibronectin , type 1 collagen , or laminin 1 . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : To understand the interaction of corneal epithelial cells with laminin , fibronectin , and collagen type 4 , major components of basement membrane , we investigated whether tyrosine phosphorylation of paxillin was increased during attachment of these cells to matrix proteins . ^^^ On a fibronectin matrix , tyrosine phosphorylation of paxillin increased in a time dependent fashion and peak time point was 6 hours of cultivation . ^^^ Paxillin was up regulated in tyrosine phosphorylation in direct relation with the fibronectin concentration . ^^^ On a type 4 collagen matrix , tyrosine phosphorylation of paxillin increased in relation to time , but not so rapidly as cultures on a laminin or fibronectin matrix . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| We , therefore , investigated the organization of actin , FAK , paxillin , and activated ERK and activated p 38 during Caco 2 motility across collagen 1 , fibronectin , laminin , and tissue culture treated glass . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Association of focal adhesion kinase with fibronectin and paxillin is required for precartilage condensation of chick mesenchymal cells . ^^^ FAK was associated with fibronectin and paxillin , which were maximal at day 3 of culture . ^^^ The beta 1 integrin antibody and several inhibitors of signaling molecules such as herbimycin A , U 73122 , LY 294002 , as well as cytochalasin D , an actin depolymerizing agent , remarkably decreased tyrosine phosphorylation of FAK and its association with fibronectin and paxillin during condensation . resulting in a marked inhibition of condensation and chondrogenesis . ^^^ Taken together , our findings suggest that beta 1 integrin mediated interaction of mesenchymal cells and fibronectin signals to accelerate the precartilage condensation through tyrosine phosphorylation of FAK and its association with paxillin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Compared with fibroblast adhesion to fibronectin , laminin , or type 1 collagen , cell adhesion to Cyr 61 or CTGF induces 1 ) more extensive and prolonged formation of filopodia and lamellipodia , concomitant with formation of integrin alpha ( 6 ) beta ( 1 ) containing focal complexes localized at leading edges of pseudopods ; 2 ) activation of intracellular signaling molecules including focal adhesion kinase , paxillin , and Rac with similar rapid kinetics ; 3 ) sustained activation of p42 / p44 MAPKs lasting for at least 9 h ; and 4 ) prolonged gene expression changes including up regulation of MMP 1 ( collagenase 1 ) and MMP 3 ( stromelysin 1 ) mRNAs and proteins sustained for at least 24 h . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Hic 5 reduced cell spreading on fibronectin : competitive effects between paxillin and Hic 5 through interaction with focal adhesion kinase . ^^^ Here , we showed that Hic 5 inhibits integrin mediated cell spreading on fibronectin in a competitive manner with paxillin in NIH 3T3 cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| These experiments suggest an important role for alpha 4 integrin and paxillin in peripheral nerve regeneration and show how alternative splicing of fibronectin may provide a mechanism to enhance repair after injury . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Inhibition of SHP 2 also markedly increased the rate of cell attachment to and cell spreading on extracellular matrix proteins such as fibronectin and vitronectin , effects that were accompanied by enhancement of adhesion induced tyrosine phosphorylation of paxillin and p130Cas . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| However , when the fibronectin is covalently linked to the substrate , alpha ( 5 ) beta ( 1 ) integrin forms highly tyrosine phosphorylated , `` classical ' ' focal contacts containing high levels of paxillin and vinculin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Within 30 min of adherence to fibronectin , human TM cells immunostained for paxillin and phosphotyrosine and exhibited prominent focal contacts . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Collagens or laminin , but not fibronectin , stimulated tyrosine phosphorylation of FAK , paxillin , and p 130 ( cas ) and activated ERK1 / 2 . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| At this time point , paxillin , a signaling component of focal adhesion complex , was predominantly localized at the focal contacts for well spread EC on fibronectin and NVP , whereas it was almost entirely concentrated in the perinuclear region of less spread cells on GBL . ^^^ However , by 24h , cells were much more spread on all three surfaces with defined stress fibers and focal contacts although EC expression of vinculin and paxillin was moderate on GBL compared to fibronectin and NVP . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| We quantitatively determined the amounts of laminin , fibronectin , vitronectin , and thrombospondin secreted by these glioma cell lines in vitro , as well as the amount of each of the eight beta integrin subunits and the adhesion complex related molecules , including talin , vinculin , profilin , zyxin , alpha actinin , paxillin , and VASP . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| TGF beta 1 up regulates paxillin protein expression in malignant astrocytoma cells : requirement for a fibronectin substrate . ^^^ TGF beta 1 stimulation of primary astrocytes and serum starved U 251MG malignant astrocytoma cells attached to fibronectin induced a substantial increase in the levels of paxillin protein ( fivefold increase at 2 . 0 ng / ml ) in a dose and time dependent manner compared to the levels observed on plating onto fibronectin in the absence of stimulation . ^^^ The TGF beta 1 stimulated increase in paxillin levels required ligation of the fibronectin receptor , as it was not induced when the cells were plated onto vitronectin , collagen or laminin . ^^^ The increase in the pool of paxillin on TGF beta 1 stimulation of the fibronectin plated astrocytoma cells was associated with an increase in translation , but was not associated with an increase in the steady state levels of paxillin mRNA . ^^^ Our results indicate that physiologic levels of TGF beta 1 stimulate the expression of paxillin protein at the level of translation through a process that requires engagement of the fibronectin receptor , and promotes attachment and spreading of malignant astrocytoma cells on fibronectin . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Of the LIM domains , LIM 2 and 3 comprise the paxillin FA targeting motif , with phosphorylation of these domains modulating paxillin targeting and cell adhesion to fibronectin ( Fn ) . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Here , endothelial GM 7373 cells spread and organize focal adhesion plaques on immobilized FGF 2 , fibronectin ( FN ) , and vitronectin ( VN ) . alpha ( 5 ) beta ( 3 ) integrin , paxillin , focal adhesion kinase , vinculin and pp 60 ( src ) localize in cell substratum contact sites on FGF 2 , FN or VN . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Interactions between human monocytes and fibronectin are suppressed by interferons beta and gamma , but not alpha : correlation with Rho paxillin signaling . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| This means that only fibronectin treated S . aureus Cowan 1 induces the accumulation of very late antigen 5 , which in turn induces the association of paxillin and tyrosine kinases . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| The adaptor protein paxillin is essential for normal development in the mouse and is a critical transducer of fibronectin signaling . ^^^ The phenotype of the paxillin ( / ) mice closely resembles that of fibronectin ( / ) mice , suggesting that paxillin is a critical transducer of signals from fibronectin receptors during early development . ^^^ Paxillin was also found to play a critical role in fibronectin receptor biology ex vivo since cultured paxillin null fibroblasts display abnormal focal adhesions , reduced cell migration , inefficient localization of focal adhesion kinase ( FAK ) , and reduced fibronectin induced phosphorylation of FAK , Cas , and mitogen activated protein kinase . ^^^ In addition , we found that paxillin null fibroblasts show some defects in the cortical cytoskeleton and cell spreading on fibronectin , raising the possibility that paxillin could play a role in structures distinct from focal adhesions . ^^^ Thus , paxillin and fibronectin regulate some common embryonic developmental events , possibly due to paxillin modulation of fibronectin regulated focal adhesion dynamics and organization of the membrane cytoskeletal structures that regulate cell migration and spreading . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Paxillin null ES cells exhibit delayed spreading on integrin binding substrates fibronectin and laminin , and there is reduced tyrosine phosphorylation of Focal Adhesion Kinase ( FAK ) . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Loss of alpha5beta1 mediated adhesion of monocytic cells to fibronectin by interferons beta and gamma is associated with changes in actin and paxillin cytoskeleton . ^^^ CONCLUSION : Our data show that IFN beta and IFN gamma induced loss of monocytic adhesion to fibronectin associated with changes in actin and paxillin cytoskeleton , thereby pointing to a possible effect of these cytokines in monocyte trafficking . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| In situ hybridization for type 1 and 2 collagens , immunohistochemical analysis for fibronectin , alpha 5 and beta 1 integrins , paxillin , and vinculin , and cytochemical staining for actin were used to demonstrate the phenotypic change of chondrocytes . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| METHODS : Triton 10 insoluble ( actin cytoskeleton ) fractions from suspension phase cells and cells adherent to the beta ( 1 ) integrin ligand fibronectin were assessed for the presence of the integrin associated kinase Pyk 2 , the scaffolding protein paxillin , and the downstream Src family kinase Lyn . ^^^ Cells adherent to fibronectin for 20 minutes demonstrated marked increases in Pyk 2 , paxillin , and Lyn activation in comparison to fibronectin adherent and suspension phase cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Examples will include studies of ECM proteins , such as fibronectin , and proteins associated with focal adhesion complexes that involve fibronectin , integrins and various intracellular modular proteins , such as focal adhesion kinase , Src family kinases , talin and paxillin . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| We report here that attenuation of ILK expression in cultured bovine aortic endothelial cells by RNA interference had marked effects on surface distribution of alpha5beta1 integrin and the organization of cell matrix adhesions characterized by the disappearance of fibrillar ( 3D like ) adhesions that are rich in alpha5beta1 and paxillin , and associated fibrillar fibronectin matrix . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| More Hic 5 , a paxillin homolog , was detected in the detergent insoluble fraction of BPAEs attached to gelatin than fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Cell lines overexpressing kinase dead ( S343A ) or paxillin binding site mutant ILK proteins display inhibited haptotaxis to fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| When AdCC cells were plated on collagen , the surface level of uPAR was increased , and numerous focal adhesions consisting of uPAR , vinculin , and paxillin were assembled ; whereas collagen stimulated SCC cell counterparts or AdCC cells plated on other types of ECM , such as fibronectin , failed to assemble such definite focal adhesions . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Myocilin binding to Hep 2 domain of fibronectin inhibits cell spreading and incorporation of paxillin into focal adhesions . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Furthermore , ablation of paxillin PABP 1 association impeded a number of indices of cell motility including spreading on fibronectin , cell migration on two dimensional matrices , and transmigration in Boyden chambers . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Moreover , co expression of CrkII and PTP1B promoted HT 1080 cell migration on fibronectin and retained tyrosine phosphorylation and binding of p130cas to CrkII , whereas paxillin tyrosine phosphorylation was reduced . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Cells cultured on fibronectin covalently linked to PVA demonstrated diminished spatial separation of paxillin and alpha ( 5 ) integrin , accompanied by a reduction in fibrillar adhesions and fibronectin fibrillogenesis . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| The initiation of somite formation coincides with an increase in the phosphorylation of Fak , and the accumulation of Fak , phosphorylated Fak , paxillin , and fibronectin at nascent somite boundaries . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| A comparison of gene expression profiles of primary tumors and lung metastatic focus between salvicine treated and untreated groups using the CLOTECH Atlas human Cancer 1 . 2 cDNA microarray revealed that genes involved in tumor metastasis , particularly those closely related to cell adhesion and motility , were obviously down regulated , including fibronectin , integrin alpha 3 , integrin beta 3 , integrin beta 5 , FAK , paxillin , and RhoC . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Adhesion dependent FAK activation on fibronectin was not affected in cells treated with the selective CaMKII inhibitor KN 93 ( 30 muM ) or in cells in which the expression of CaMKII with small interfering RNA ( siRNA ) was suppressed , although tyrosine phosphorylation of paxillin was inhibited in CaMKII delta ( 2 ) suppressed cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| In human gingival fibroblasts plated on fibronectin , IL 1beta enhanced the maturation of focal adhesions as defined by morphology and enrichment with paxillin and alpha actinin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Interestingly , C2GnT M inhibited cell adhesion to collagen 4 and fibronectin , and decreased tyrosine phosphorylation of paxillin , indicating that the changes in cancer behavior may be partly mediated by integrin signaling pathways . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Assembly of native FN induced a rapid reorganization of actin into stress fibers and colocalization of alpha 5 beta 1 integrin , focal adhesion kinase ( FAK ) , vinculin , and paxillin to regions of cell matrix contact . alpha 5 beta 1 integrins and FAK are also clustered upon binding of FN delta III 1 7 to cells but actin reorganization and focal adhesion formation are delayed and appear to be dependent on the formation of FN delta III 1 7 fibrils . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| While overexpression of Csk or the Csk mutants did not significantly affect cell adhesion to the Fn surface or alpha 5 integrin recruitment to the attachment sites , Csk suppressed and mCsk almost abolished Fn mediated tyrosine phosphorylation of paxillin , filamentous actin assembly to podosomes , and cell migration , but mCsk ( ) did not . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| In addition , we show that FAK and MAP kinase belong to distinct integrin signaling pathways and that both pathways remain functional during Caco 2 cell differentiation since the coating of differentiating cells on FN and LN but not on polylysine increased the tyrosine phosphorylation of FAK and of its endogenous substrate paxillin , and stimulated MAP kinase activity . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Expression of various FAK mutants in the FAK cells showed that FAK kinase activity , the Tyr 397 / SH2 domain binding site , and the first proline rich SH 3 binding region in the FAK C terminal domain were individually needed to promote full FAK mediated FAK cell migration to FN whereas direct paxillin binding to FAK was not required . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| LPA and FN also evoked the formation of focal adhesions and actin bundles , and tyrosine phosphorylation of focal adhesion kinase ( FAK ) and paxillin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Integrin activation by immobilized FN induced phosphorylation of pp 125 focal adhesion kinase and paxillin and induced the formation of focal adhesion kinase / Grb 2 / Sos complex . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Collectively , these data suggest CadF promotes the binding of C . jejuni to Fn , which in turn stimulates a signal transduction pathway involving paxillin . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| As a result of FN binding by the beta 1EhFNR , the receptor itself , FAK , and paxillin were phosphorylated in tyrosine . ^^^ Co immunoprecipitation experiments showed that a multimolecular signaling complex was formed by the amoebic FN receptor , FAK , paxillin , and vinculin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| In contrast , the focal adhesion protein paxillin was not phosphorylated at Tyr 31 with FN . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| RESULTS : CN binding to integrins can mimic the intracellular signaling cascade evoked by FN , because the phosphorylation of the key signaling proteins focal adhesion kinase , paxillin , and p 130 Crk associated substrate and the association of Src with focal adhesion kinase are similar . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| Consequently , tyrosine phosphorylation of paxillin was obviously persistent after stimulation with FN + LPA as compared to after stimulation with either alone . ^^^ Immunoblotting of anti paxillin immunoprecipitates with phosphorylation site specific Abs revealed the following : tyrosine phosphorylation was enhanced preferentially on a slowly migrating component after stimulation with FN + LPA ; this component contained phosphorylation at both tyrosine residue ( Y ) 31 and Y 118 ; and phosphorylation of paxillin at Y 181 was constitutive and not augmented by stimulation with either FN or LPA . ^^^ Paxillin phosphorylation weakly induced by FN alone , insufficient for cell migration , was not inhibited by amiloride . ^^^ These results demonstrate that LPA collaborates with FN for persistent tyrosine phosphorylation of paxillin at both Y 31 and Y 118 , regulated by the Na+ / H+ antiporter downstream of ROCK and that this phosphorylated paxillin is essential for MM 1 cancer cell migration . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P49023 |
Pubmed |
SVM Score :0.0 |
| NA |
|