| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.52719794 |
| The VHL gene product , pVHL , forms multiprotein complexes that contain elongin B , elongin C and Cul 2 , and negatively regulates hypoxia inducible mRNAs . pVHL is suspected to play a role in ubiquitination given the similarity of elongin C and Cul 2 with Skp 1 and Cdc 53 , respectively . pVHL can also interact with fibronectin and is required for the assembly of a fibronectin matrix . 0.52719794^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Using recently available markers to macrophages and microglia ( monoclonals Mac 387 , KP 1 and the lectin RCA 1 ) and antisera to GFAP , fibronectin and laminin , we have described the reactions of these cells and the extracellular matrix . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| We compared recently available markers for the detection of macrophages and microglia ( monoclonals Mac 387 , KP 1 and the lectin RCA 1 ) and also used antisera to GFAP , fibronectin and laminin . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| The architecture of the ECM was revealed by silver and lectin staining ( peanut and Ricinus communis agglutinins , PNA and RCA 1 ) , by immunocytochemistry ( for fibronectin , tenascin , collagen type 1 ) and by ultrastructural analysis . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Monospecific antibodies to laminin and fibronectin and the affinity of the lectins for mannosyl , glucosyl , and galactosyl residues with RCA 1 . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| A panel of antibodies , including those against Thy1 . 1 , smooth muscle actin , desmin , cellular fibronectin and beta 1 integrin alpha 1 and alpha 5 chains , and Wistaria floribunda ( WFA ) and Ricinus communis ( RCA 1 ) lectins , were found useful for mesangial cell detection in vivo and in vitro . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Fibronectin coimmunoprecipitated with wild type von Hippel Lindau protein ( pVHL ) but not tumor derived pVHL mutants . ^^^ Immunofluorescence and biochemical fractionation experiments showed that fibronectin colocalized with a fraction of pVHL associated with the endoplasmic reticulum , and cold competition experiments suggested that complexes between fibronectin and pVHL exist in intact cells . ^^^ These data support a direct role of pVHL in fibronectin matrix assembly . . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| The morphological changes of the WT VHL spheroids were associated with the deposition of fibronectin in the extracellular space , a feature that was absent in the mutant and inactivated VHL gene expressing spheroids . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| The VHL protein appears to have several distinct functions : ( a ) down regulation of hypoxia inducible mRNAs ; ( b ) proper assembly of the extracellular fibronectin matrix ; ( c ) regulation of exit from the cell cycle ; and ( d ) regulation of expression of carbonic anhydrases 9 and 12 . . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| The VHL gene product is reported to form complexes with various proteins including elongin B , elongin C , VBP 1 , fibronectin , Spl , CUL 2 , and HIF 1 . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Exon 2 encoded residues are required for VHL mediated NEDD 8 conjugation on cullin 2 and assembly with hypoxia inducible factor alpha ( HIFalpha ) and fibronectin . ^^^ Exon 3 encoded alpha helical domain is required for VHL complex formation with BC / cullin 2 and E 3 ubiquitin ligase activity , for binding to HIFalpha / fibronectin , but this domain is not essential for transcription dependent nuclear / cytoplasmic trafficking . ^^^ VHL ( / ) renal carcinoma cells expressing beta domain mutants failed to produce an extracellular fibronectin matrix and to degrade HIFalpha , which accumulated exclusively in the nucleus of normoxic cells . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Here we report that the products of such type 2C VHL alleles retain the ability to down regulate HIF but are defective for promotion of fibronectin matrix assembly . ^^^ These studies strengthen the notion that HIF deregulation plays a causal role in hemangioblastoma and renal carcinoma , and raises the possibility that abnormal fibronectin matrix assembly contributes to pheochromocytoma pathogenesis in the setting of VHL disease . . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| According to recent studies , the VHL protein ( pVHL ) is a multifunctional tumor suppressor protein associated with the inhibition of angiogenesis , cell cycle exit , fibronectin matrix assembly , and proteolysis . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Evidence for further pVHL functions including roles in fibronectin metabolism and cell cycle regulation has also been reported , but it is unclear whether these functions are mediated via pVHL targeted proteolysis or other mechanisms . ^^^ |
|
| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| VHL is requiredfor the assembly of a proper extracellular fibronectin matrix , although the exact mechanism remains unknown . ^^^ Because the formation of integrin fibrillar adhesions plays a pivotal role in the organization of extracellular fibronectin , we next examined the expression and subcellular distribution of integrins in VHL cells and their wild type VHL stably transfected counterparts . ^^^ Exogenous activation of beta 1 integrins with either divalent cations or activating antibodies partly restored the capability of VHL cells to assemble beta 1 fibrillar adhesions and fibronectin fibers . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Fibronectin was not stabilized like Jade 1 by VHL , nor were candidate VHL interactors from a yeast screen . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| VHL modification by NEDD 8 is required for fibronectin matrix assembly and suppression of tumor development . ^^^ Functional inactivation of the von Hippel Lindau ( VHL ) tumor suppressor gene is the cause of the familial VHL disease and most sporadic renal clear cell carcinomas ( RCC ) . pVHL has been shown to play a role in the destruction of hypoxia inducible factor alpha ( HIF alpha ) subunits via ubiquitin mediated proteolysis and in the regulation of fibronectin matrix assembly . ^^^ Although most disease causing pVHL mutations hinder the regulation of the HIF pathway , every disease causing pVHL mutant tested to date has failed to promote the assembly of the fibronectin matrix , underscoring its potential importance in VHL disease . ^^^ These results suggest that NEDD 8 modification of pVHL plays an important role in fibronectin matrix assembly and that in the absence of such regulation , an intact HIF pathway is insufficient to prevent VHL associated tumorigenesis . . ^^^ A nonneddylateable pVHL mutant , while retaining its ability to ubiquitylate HIF , failed to bind to and promote the assembly of the fibronectin matrix . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| We propose that phosphorylation of the acidic domain plays a role in the regulation of proper fibronectin matrix deposition and that this may be relevant for the development of VHL associated malignancies . . ^^^ This post translational modification did not affect the levels of hypoxia inducible factor ; however , it did change the binding of pVHL to another known binding partner , fibronectin . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Stable expression of shRNA against EGFR does not alter characteristics associated with VHL loss including constitutive production of HIF targets and defects in fibronectin deposition . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| We found that the impaired migration and adhesion of VHL null endothelial cells can be partially rescued by the addition of back exogenous fibronectin , which indicates that pVHL regulation of fibronectin deposition plays an important functional role in vascular patterning and maintenance of vascular integrity . . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| Fibronectin is a hypoxia independent target of the tumor suppressor VHL . ^^^ The von Hippel Lindau ( VHL ) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition . ^^^ Among the differentially expressed genes , we detected that fibronectin is upregulated in the presence of VHL , while it is not affected by hypoxia . ^^^ Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level , irrespective of oxygen levels . . ^^^ |
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| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P40337 |
Pubmed |
SVM Score :0.0 |
| NA |
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