| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.6333874 |
| Fibronectin was neither associated with vinculin containing focal contacts of Nil 8 cells cultured in medium with 5 % FBS nor with vinculin negative focal contacts located at the extreme edges of stationary cells arrested in 0 . 3 FBS . 0.6333874^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.6044877 |
| Additional labeling experiments revealed that beta 1 integrin and fibronectin are confined to the adherens junctions , as indicated by their codistribution with vinculin and tensin . 0.6044877^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In contrast , the cells on fibronectin and type 1 collagen formed complete F actin filaments , exhibited sufficient vinculin and beta 1 integrin , and grew logarithmically from 2 days . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The possible usefulness of these markers in patient evaluations was supported by our finding that the distribution of vinculin , fibronectin , myosin , actin B 4 , desmin , glomerular HLA DR , and the tubular expression of CD 15 remained unchanged . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| By using indirect immunofluorescence , we found that papillary MTJs more closely resemble skeletal MTJs in their molecular composition in that they are enriched in talin , vinculin , integrin , and fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| An immunological approach has previously implicated a transmembrane interaction between fibronectin and vinculin in the control of odontoblast differentiation . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Fibroblasts spread within 1h on substrata composed of fibronectin and formed focal adhesions by 3h , as monitored by interference reflection microscopy ( IRM ) and by labeling for talin , vinculin and integrin beta 1 subunits . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| These results demonstrate that vinculin and beta 1 integrin , important components of the cell adhesion apparatus , are members of a group of immediate early growth responsive genes , along with c fos , c myc , actin , and fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The protein appears to be located in focal contacts where it codistributes with beta 1 integrins . pp 120 is distinct from the beta 1 subunit of integrins and from vinculin and alpha actinin . pp 120 is rapidly dephosphorylated in cells suspended by trypsinization but becomes rapidly phosphorylated in cells attaching and spreading on fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| When basement membrane glycoproteins laminin or fibronectin were exogenously supplied by growing cells on coated coverslips , the vinculin , talin and integrin containing contacts became organized into linear arrays with intervening free cell membranes . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The integrin complex alpha 5 beta 5 had a topography similar to that of talin and vinculin containing focal adhesions mostly in the peripheral cells of expanding keratinocyte colonies and in coincidence with fibronectin strands . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Immunocytochemical investigations performed on menadione exposed cells revealed that some surface proteins ( collagen 4 , sialo proteins , beta 2 microglobulin and fibronectin ) and adhesion molecules ( vinculin ) underwent changes in their expression over the bleb surface . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Examination of immunolabelled primary hepatocytes spread on fibronectin coated substrata by light and laser scanning confocal microscopy revealed colocalization of AGp 110 , integrin , actin and vinculin in substratum attached microextensions at the periphery of the basal cell surface . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| A model of the structure of the adhesion plaque is given , considering some recent data on proteins which are involved : adhesion proteins ( collagen , fibronectin , laminin etc . ) , receptors of adhesion proteins ( e . g . integrin ) , components of the membrane skeleton ( talin , vinculin etc . ) , and cytoskeletal proteins . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| A 165 kDa membrane antigen mediating fibronectin vinculin interaction is involved in murine odontoblast differentiation . ^^^ In this study , we analyzed the interactions of vinculin and fibronectin with plasma membrane proteins separated by sodium dodecyl sulphate ( SDS ) polyacrylamide gel electrophoresis , and then transferred onto polyvinylidene difluoride ( PVDF ) paper . ^^^ Attempts were made to characterize the 165 kDa protein which interacted with vinculin and with fibronectin . ^^^ The data presented in this paper and previous observations indicate that the 165 kDa protein , involved in odontoblast elongation and polarization , mediates a fibronectin vinculin transmembrane interaction . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Only the integrin complex alpha 5 beta 5 was associated with talin and vinculin containing focal adhesions mostly in the peripheral cells of expanding keratinocyte colonies and in coincidence with fibronectin strands . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In the present study we found , using immunofluorescent staining , that beta 1 integrin complexes were colocalized with vinculin in focal adhesion plaques on laminin , type 4 collagen , and fibronectin substrates . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| This was demonstrated in several differentiating systems : in 3T3 preadipocytes which change their shape from a fibroblastic to a spherical shape when stimulated to differentiate with adipogenic medium , we observed a decrease in mRNA levels and in the synthesis of fibronectin , beta integrin , and the microfilament proteins , vinculin , alpha actinin , tropomyosin and actin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| With the exception of calpactin 1 , the major tyrosine phosphoproteins detected in immunoblots appeared to be different from several previously characterized substrates of pp60v src with similar molecular masses ( ezrin , vinculin , and the fibronectin receptor ) . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Focal contacts containing either the fibronectin or vitronectin receptor were both associated with the termini of stress fibres and contained the proteins talin and vinculin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The distribution of alpha actinin , vinculin , alpha spectrin , beta spectrin and fibronectin was analyzed in 14 to 21 week old fetal human inner ears using immunofluorescence microscopy . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The cultures were double stained with various combinations of the specific antiactin drug phalloidin and antibodies against vinculin , alpha actinin , connectin ( titin ) , myosin heavy chain , fibronectin , and desmin and examined under fluorescence and interference reflection microscopy . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Integrin localization was compared with that of its putative cytoskeletal associated and extracellular matrix ligands , talin and vinculin and fibronectin and laminin , respectively . ^^^ The localization of integrin on developing and adult muscle tissue was compared with that of fibronectin , laminin , vinculin , and talin using double , immunofluorescently labeled cryosections . ^^^ On posthatch tissue , the distributions of talin , vinculin , laminin , and fibronectin were similar to those in the embryo , whereas the distribution of integrin was restricted to specific sites . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Within 6 h on fibronectin many cells expressed vinculin containing focal contacts , elaborated stress fibers and had periodically organized alpha actinin , whereas on laminin , most cells showed diffuse vinculin and alpha actinin and a fine meshlike actin cytoskeleton . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| After 30 min , when many of the cells were at a more advanced stage of spreading around beads or phagocytosing beads , alpha actinin and actin , but not vinculin , form distinctive aggregates at sites along membranes associated with either fibronectin or Con A beads . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Actin reorganization provoked by forskolin coincide with redistribution of vinculin to the cell periphery and rapid elimination of surface associated fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Similarly , in teratocarcinoma derived PE cells ( PYS 2 ) adhesion to fibronectin induced the formation of distinct bundles of F actin and plaques of vinculin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In the present paper we show that over the concentration range tested ( 0 . 1 10 ng / ml ) TGF beta has no effect on RPE cell proliferation either in the presence or the absence of serum , cell motility and the organization of cytoskeleton extracellular matrix linkage complexes with respect to their structure and presence of actin , vinculin , talin , integrin and fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| When confluent preadipocyte cultures were stimulated with adipogenic medium there was a gradual decrease in the expression of fibronectin , beta integrin , actin and in the microfilament associated proteins vinculin , alpha actinin and tropomyosin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| As yet , there is no detailed knowledge of the molecules involved in this transmembrane linkage , although several proteins have been suggested as candidates in the chain of attachment between bundles of actin filaments and the cytoplasmic face of the plasma membrane : these include vinculin , alpha actinin and talin , each one having been identified at regions where bundles of actin filaments interact with the plasma membrane and underlying cell surface fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The ASML cells also synthesized very low levels of both vinculin and fibronectin , displayed a diffuse pattern of actin and tubulin organization , and were unable to spread on the substrate . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The absence of pp60src from the adhesion plaques was not due to lack of myristylation of the src protein , and tyrosine phosphorylation of vinculin was not related to fibronectin expression . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Comparison of the relative importance of tyrosine specific vinculin phosphorylation and the loss of surface associated fibronectin in the morphology of cells transformed by Rous sarcoma virus . ^^^ We have investigated the relative importance of tyrosine specific phosphorylation of vinculin and the loss of surface associated fibronectin in the maintenance of the rounded morphology characteristic of chick embryo fibroblasts ( CEF ) transformed by Rous sarcoma virus ( RSV ) . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Cytoskeletal organization , vinculin phosphorylation , and fibronectin expression in transformed fibroblasts with different cell morphologies . ^^^ The distribution of vinculin , alpha actinin , actin , and surface fibronectin was studied , and , where appropriate , also the extent of phosphotyrosine modification of vinculin . ^^^ Irrespective of the transforming agent we found that increased cell rounding was generally correlated with a reduction in vinculin containing focal adhesions , a dissolution of microfilament bundles , and a reduction of extracellular fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Vinculin was confined to the cytoplasmic aspect of the plasmalemmal termination sites of stress fibers , while laminin , fibronectin , and collagens were located at the extracellular aspect of these stress fiber membrane associations . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Prolonged incubation with TPA allowed P cells to regain their original appearance and resulted in growth inhibition ; however , the extended presence of TPA produced in P+ cells persistent alterations in the distribution of actin , vinculin , and fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In this report , we describe the relative distribution of vinculin , talin , and fibronectin in cultured retinal pigmented epithelial cells from chick embryo eyes . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In the chicken kidney , the plasmalemmal portion of the foot processes that abuts the glomerular basement membrane was specifically labeled with antibodies against chicken gizzard vinculin and talin , two proteins thought to be important for the linkage of actin filaments to the lipid bilayer and to the receptor for fibronectin and laminin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Hmf n cells have polar stress fiber arrays terminating in vinculin adhesion plaques , colinear extracellular fibronectin matrices , and linear non coincident deposits of fodrin . ^^^ Stress fibers of the tHmf f are moderately reduced , their vinculin adhesion plaques and fibronectin matrices intact ; fodrin is diffuse . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The role of actin binding proteins vinculin , filamin , and fibronectin in intracellular and intercellular linkages in cardiac muscle . ^^^ The localization in cardiac muscle and the biochemical properties of fibronectin , filamin , and vinculin were studied . ^^^ It was suggested that fibronectin , filamin , and vinculin play an important role in intercellular and intracellular linkages in cardiac muscle . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In prefusion myoblasts , which are rapidly detached by CSAT Mab , CSAT ag is distributed diffusely as are vinculin , laminin , and fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In the present study , we demonstrate that the actomyosin vinculin system of the monensin exposed cells can be restored by plating the cells on the culture substratum coated with cellular fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The distribution of microfilament bundles , intermediate filaments , microtubules , vinculin plaques , and extracellular fibronectin matrices was examined by indirect immunofluorescence in 10 ray transformed C3H / 10T1 / 2 cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| On the other hand , the amounts of vinculin and fibronectin in dl 5 transformed cells were twice those in wt RSV transformed cells , although less than the amounts in uninfected cells . ^^^ These results suggest that events leading to cellular morphological transformation are neither directly correlated with phosphorylation of the 36K protein , with dephosphorylation of the 55K and 57K proteins , nor with the level of phosphorylation of tyrosine residues in vinculin , but rather are correlated with the amount of vinculin and fibronectin . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| A series of partial transformation mutants of RSV that induce distinct transformation phenotypes were used , and infected chicken embryo cells were examined for ( 1 ) intracellular pp60src location , ( 2 ) vinculin localization , ( 3 ) abundance of phosphotyrosine on vinculin , ( 4 ) integrity of stress fibers , and ( 5 ) expression of cell surface fibronectin . ^^^ The results indicate that , among the limited number of mutants studied here , the presence of pp60src in adhesion plaques is independent of growth in soft agar and the increased phosphorylation of vinculin on tyrosine , but it does correlate with the loss of cell surface fibronectin . ^^^ An elevated abundance of phosphotyrosine on vinculin is insufficient to cause stress fiber dissolution and is independent of the loss of fibronectin from the extracellular matrix . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Thus the morphological response to TPA involves rapid redistribution of actin and vinculin independent of transcription and translation , fluctuations in the levels of calcium or cyclic AMP , or changes in the organization of microtubules , intermediate filaments , and fibronectin . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In addition , immunofluorescent analyses showed a correlation between vinculin localization and the negative fibronectin spots found under transformed cells , indicating that degradation occurs at cell substratum contact sites . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Polyacrylamide gel electrophoresis of [ 35S ] methionine labeled fibronectin and vinculin immunoprecipitated from lysates of normal and transformed cells indicated that cell associated fibronectin synthesized during the labeling period is reduced by 60 % in RSV transformed cells but occurs in normal amounts in UR 2 transformed cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| We demonstrate interaction between isolated actin and alpha actinin but not vinculin with fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| A transmembrane relationship between fibronectin and vinculin ( 130 kd protein ) : serum modulation in normal and transformed hamster fibroblasts . ^^^ Since vinculin , a recently discovered intracellular protein , is localized at the membrane insertion regions of actin fibers , we studied its possible relationship to fibronectin and the fibronexus . ^^^ Using double label immunofluorescence microscopy , we have observed that the distributions of vinculin and fibronectin are strikingly coincident in normal Nil 8 hamster fibroblasts arrested in the G 1 phase of the cell cycle , and in HSV transformed Nil hamster cells treated with purified fibronectin after culturing in 0 . 3 % serum . ^^^ Extensively spread Nil 8 cells have numerous vinculin positive focal patches , which are localized either directly over or in tandem with fibronectin fibers at the ventral surface . ^^^ However , fibronectin and vinculin do not exhibit this relationship in Nil 8 cells grown in 5 % serum . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Fibronectin has an affinity to vinculin , alpha actinin , tropomyosin and myosin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| We have carried out double immunoelectron microscopic labeling experiments on ultrathin frozen sections cut through such contact sites to determine the absolute and relative dispositions of the three proteins fibronectin , vinculin , and alpha actinin with respect to these sites . ( a ) Three types of cell substratum and cell cell contact sites familiar from plastic sections could also be discriminated in the frozen sections by morphological criteria alone , i . e . , the gap distances between the two surfaces , and the presence of submembranous densities . ^^^ Control experiments showed that this could not be ascribed to inaccessibility of the FA narrow gap to the immunolabeling reagents but indicated an absence or severe depletion of fibronectin from these sites . ( c ) CC sites were labeled intracellularly for alpha actinin but not vinculin and were labeled extracellularly for fibronectin . ( d ) ECM sites were characterized by large separations ( often greater than 100 nm ) between the cell and substratum or between two cells , which were connected by long cables of extracellular matrix components , including fibronectin . ^^^ ECM sites appeared to be of two kinds , one labeled intracellularly for both alpha actinin and vinculin , the other for alpha actinin alone . ( e ) From these and other results , a coherent but tentative scheme is proposed for the molecular ultrastructure of these contacts sites , and specific functional roles are suggested for fibronectin , vinculin , and alpha actinin in cell adhesion and in the linkage of intracellular microfilaments to membranes at the different types of contact sites . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Thus , in addition to having similar substratum associated glycoproteins ( ( eg , fibronectin ) and cytoskeleton associated proteins ( eg , alpha actinin and vinculin ) cells from different species and tissue sources appear to have a relatively conserved class of integral membrane glycoproteins involved in cell substratum adhesion . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The adhesion and spreading of human gingival fibroblasts on glass and differently processed titanium surfaces was studied by immunolocalization of vinculin and the alpha and beta subunits of the fibronectin ( alpha 5 beta 1 ) and ( alpha 5 beta 3 ) receptors . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| As in normal plaques , talin and vinculin were present , the plasma membrane was very close ( 10 20 nm ) to the substratum and the fibronectin layer underneath was removed . ^^^ These observations indicate that the talin vinculin network of an adhesion plaque is normally anchored to the substratum at discrete beta 1 integrin clusters that may be located on fibronectin fibrils , and that elsewhere the plaque is not necessarily attached to the substratum by interaction of integrins with matrix proteins . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Synchronous appearance of fibronectin , integrin alpha 5 beta 1 , vinculin and actin in epithelial cells and fibroblasts during rat tracheal wound healing . ^^^ Under anesthesia , the ventral surface of the trachea was scratched , and tissue specimens were obtained from 6 h to 3 weeks after injury and studied using light and electron microscopy and immunohistochemistry . alpha 5 beta 1 , vinculin and actin in regenerating epithelial cells and extracellular fibronectin appear virtually simultaneously after injury ( from 12 h to 7 days ) as do alpha 5 beta 1 , vinculin and alpha smooth muscle actin in fibroblasts and cellular fibronectin in granulation tissue ( from 3 to 10 days ) . ^^^ Immunoelectron microscopy 2 days after injury showed that alpha 5 beta 1 and vinculin were localized on the basal and lateral surfaces of regenerating epithelial cells and fibroblast surfaces , and fibronectin was localized just under the regenerating epithelial cells , around collagen fibrils and sporadically around fibroblasts . ^^^ The appearances of fibronectin , alpha 5 beta 1 , vinculin and actin in regenerating epithelial cells and fibroblasts during tracheal wound healing are well coordinated . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Double label immunofluorescence studies indicated that , in static culture , alpha 5 beta 1 fibronectin receptors ( alpha 5 beta 1 FNRs ) and alpha 5 beta 3 vitronectin receptors ( alpha 5 beta 3 VNRs ) were found predominantly in the peripheral regions of bovine aortic endothelial cells ( BAECs ) corresponding to the localization of vinculin , talin , and actin microfilament terminations . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| We found that vinculin , talin , paxillin , a fibronectin receptor protein , several integrin subunits including beta 1 , fibronectin , and proteins with phosphorylated tyrosine were also components of the apical plaque . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Focal adhesion complexes ( FACs ) containing integrin beta 1 , talin , vinculin , talin , alpha actinin , and paxillin formed within 15 min when round cells bound magnetic microbeads coated with integrin ligands , such as fibronectin or RGD containing peptide , but not when coated with acetylated low density lipoprotein . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The pathophysiological role of the cytoskeleton and the extracellular matrix are well defined for beta tubulin , fibronectin , laminin , desmin , vimentin , vinculin and collagen : beta tubulin is increased or altered in dilated cardiomyopathy ( DCM ) . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Cells on fibronectin exhibited co distribution of immunostaining for talin , vinculin , IL 1 receptor , and focal adhesion kinase ( pp125fak ) in focal adhesions and demonstrated [ Ca2+ ] 1 responses with 10 ( 8 ) M IL 1 beta . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Biochemical analysis has shown a correlation between tyrosine specific phosphorylation of the fibronectin receptor and the loss of surface bound fibronectin , but no such correlation with phosphorylation of vinculin or talin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The genes coding for different types of collagens , laminins , fibronectin , fibrillins , desmin , titin , alpha actinin , vinculin , cardiac and skeletal alpha actins , ankyrin and spectrin were excluded as responsible for FHC . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The cellular morphological changes were analyzed and correlated with the distribution of cell substratum contacts viewed by confocal images obtained after immunostaining with antibodies raised against the fibronectin receptor , talin , vinculin and actin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| PG M was abundant in the subcellular space of fibroblasts , but was excluded selectively from focal contacts where vinculin , integrins and fibronectin were localized . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Focal contact associated proteins such as fibronectin receptors , vitronectin receptors and vinculin were shown in this study to play specific roles in the modulation of the cytoskeletal and cell shape changes . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Absence of interaction between the 165 kDa fibronectin binding protein involved in mouse odontoblast differentiation and vinculin . ^^^ Previous data suggested that matrix could control the organization of microfilaments in differentiating odontoblasts and that this process involved a complex of fibronectin 165 kDa membrane protein vinculin . ^^^ The use of two different gel systems and microsequence analysis demonstrated that two distinct 165 kDa proteins interact , one with fibronectin and the other with vinculin . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The purpose of the present study was to determine changes in the localization of vinculin , Type 4 collagen , laminin , and fibronectin during the damage repair process in the skeletal muscle over several days following acute blunt trauma . ^^^ Further , when small newly formed or splitted myofibers were observed in the areas of regenerating myofibers ( day 10 after trauma ) , vinculin , Type 4 collagen , laminin , and fibronectin were localized in specialized regions corresponding to the costamere , basement membrane , and endomysium . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In the radially spread fibroblasts , fibronectin receptors and vinculin colocalized in focal adhesion sites and appeared to be membrane insertion points for circumferentially arranged actin filament bundles , but these sites were much smaller than the focal adhesion plaques in polarized cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Furthermore , when cells were cultured on fibronectin , a substrate not recognized by the alpha 6 beta 1 laminin receptor , the distribution of the two alpha 6 isoforms was similar to that observed on laminin , with alpha 6A still colocalizing with vinculin in focal adhesions . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| HTSC express CD 54 ( ICAM 1 ) , CD49a ( VLA 1 ) , CD49b ( VLA 2 ) , CD49c ( VLA 3 ) , CD49e ( VLA 5 ) , CD49f ( VLA 6 ) , CD 29 , CD 51 , CD 44 and produce vinculin , beta tubulin , alpha actin , vimentin , fibronectin , laminin and collagen types 1 , 3 and 4 . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Surface ruffling , actin aggregation , vimentin retraction , rearrangement of vinculin in focal adhesions , and fibronectin extraction were apparent . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Immunofluorescence microscopy of cells grown on fibronectin confirmed the presence of paxillin , talin , and vinculin at the ends of actin stress fibers at presumptive focal contacts in melanocytes . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Our data indicated that , while talin , vinculin , paxillin , fibronectin receptor and integrin beta 1 were present at the three stress fiber membrane association sites , vitronectin receptor and integrin alpha 5 were absent from the apical plaque and the lateral association site . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In contrast , differences were observed for vinculin distribution within cells spread on fibronectin or on laminin 1 . ^^^ Following cell adhesion to fibronectin most of the vinculin appeared as thick patches at the tips of the actin stress fibers while in laminin adhering cells vinculin was recruited into thin streaks localized at the end of only some actin stress fibers . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| BMSC expressed fibronectin , alpha actin , beta tubulin , vimentin and vinculin while cytokeratinpan , GFAP , neurofilament , desmin and laminin were not expressed . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In this report , we have characterized the effect of stem cell factor on the organization of the actin cytoskeleton in human melanocytes attached to fibronectin , and have examined the effect of stem cell factor on the phosphorylation of the focal contact protein paxillin and on the expression of the focal contact proteins talin , paxillin , vinculin , and alpha actinin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical staining showed that fibronectin was detected in the extracellular matrix ( and partially on the surface of tumor cells ) , integrin alpha 5 beta 1 on the cell surface , and vinculin and actin were detected in the cytoplasm of the tumor cells . ^^^ We conclude that the expression of fibronectin , integrin alpha 5 beta 1 , vinculin , cytoplasmic actin and fibronexus like structure in leukemic cells indicate these cells possess an adhesive function . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Matrix nucleation sites on newly spread cells were visualized using Texas Red conjugated 70 kDa fragment and were found to colocalize with vinculin and substrate fibronectin fibrils . ^^^ Similarly , exogenous fibronectin conjugated with Texas Red was also colocalized with vinculin when added to newly adherent cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Cells plated on poly ( l lysine ) and incubated with fibronectin coated beads exhibited vinculin staining in association with the beads . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Immunoblot assay using antibodies showed increased expression of fibronectin , fibronectin receptors , and vinculin on MSB 1 41C cell lines . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| However , unique to the HUVEC plated on growth inhibitory concentrations of fibronectin was a redistribution in situ of integrins and vinculin to form more numerous focal adhesions , and an increased polymerization of cytoskeletal actin to form stress fibers . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Furthermore , colocalization of RAFTK protein with vinculin , a focal adhesion protein , was observed by confocal microscopy in focal adhesion like structures in adherent CMK cells and in transfected pCDNAIII / flag RAFTK COS cells upon fibronectin activation . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| To provide a better understanding of the role of fibroblastic cells during corneal wound healing , we examined the expression of cytoskeleton components ( i . e . smooth muscle alpha actin ( alpha SMA ) , vimentin , desmin ) , adherens molecules ( vinculin and talin ) and cellular fibronectin in alkali burned and lacerated rabbit corneas . ^^^ Alkali burned and lacerated corneas , which had healed for various periods of time ( 1 day to 45 days ) , were excised and subjected to immunohistochemical studies with monoclonal antibodies against alpha SMA , vimentin , desmin , vinculin , talin and cellular fibronectin . ^^^ Antibodies against cellular fibronectin , vinculin and talin react with the fibroblastic cells in the injured corneas , but not with the keratocytes of normal corneas . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The proteins encoded by these clones could be grouped into four distinct classes : extracellular matrix proteins ( fibronectin , beta ig h 3 , collagen 6 ) , enzymes ( collagenase , urokinase ) , cytoskeletal proteins ( vinculin , SM 22 ) and regulatory proteins ( beta glycan , integrin associated protein , myosin kinase , IGFBP 5 ) . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Messenger RNA analysis confirmed that the changes in accumulation and protein synthesis observed for vinculin , fibronectin , and osteopontin were controlled at a pretranslational level . ^^^ In conclusion , the elevated rate of synthesis and the increased accumulation of vinculin and fibronectin , as well as the increase in the number and size of stress fibers and focal adhesion complexes , suggest that mechanical strain leads to a coordinated change both in the cytoskeleton and in extracellular matrix proteins that will facilitate tighter adhesion of an osteoblast to its extracellular matrix . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| By immunohistochemistry and western blotting using the antibodies against integrin ( VLA 5 ) , talin , vinculin , fodrin , vimentin , ankyrin , plectin , fibronectin , and focal adhesion kinase ( FAK ) , there was no significant difference in localization and amount between the groups . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Moreover immunofluorescence on fibroblasts obtained from culture plates showed increased actin stress fibers and fibronectin production with low stimulation by GM CSF , while higher concentration of this cytokine determined increased proliferation of cells , but a decreased formation of actine fibers and vinculin plaques . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Double label immunofluorescence studies with antibodies specific for clathrin , vinculin and several forms of integrin showed that focal contacts and clathrin coated membrane domains contain both vitronectin receptors ( VnR , containing beta 3 and beta 5integrins ) and fibronectin receptors ( FnR , containing beta 1 integrin ) . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Buttons were single and double labeled using phalloidin and antibodies to ZO 1 , Ki 67 , fibronectin , alpha smooth muscle ( SM ) actin , and vinculin . ^^^ RESULTS : Organ culture in TGF beta 1 , beta 2 , or beta 3 induced myofibroblast transformation of corneal endothelial cells , with formation of stress fibers containing alpha SM actin , loss of normal pericellular ZO 1 organization , development of extracellular fibronectin fibrils , and formation of focal contacts as indicated by punctate vinculin staining . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Target cells had ultrastructural features typical of smooth muscle and were immunoreactive for alpha smooth muscle actin , smooth muscle myosin heavy chain , desmin , vinculin , and laminin , but not non muscle myosin , vimentin , fibronectin , or type 4 collagen ; nerve growth factor ( NGF ) mRNA was detected by reverse transcription polymerase chain reaction . ^^^ Periorbital sheath devoid of sympathetic nerves contained elongated fibroblasts that were immunoreactive for vimentin , non muscle myosin , and fibronectin , but not for alpha smooth muscle actin , smooth muscle myosin heavy chain , vinculin , desmin , laminin , or type 4 collagen , and did not express NGF mRNA . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Talin , vinculin , FAK , and alpha 5 beta 6 integrin , the fibronectin receptor , were clustered in focal contacts , which agrees with a possible role of fibronectin in final cell growth , the latter process mediating the final phase of differentiation . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| When plated on fibronectin , PTP PEST ( / ) fibroblasts display a strong defect in motility . 3 h after plating on fibronectin , the number and size of vinculin containing focal adhesions were greatly increased in the homozygous PTP PEST mutant cells as compared with heterozygous cells . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Undifferentiated talin ( / ) ES cell mutants are unable to assemble focal adhesions when plated on fibronectin , whereas vinculin ( / ) ES cells are able to do so . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The stress fibres direct the initial elongation of cells and control the deposition of oriented extracellular matrix via junctional complexes with the matrix involving vinculin and alpha 5 beta 1 integrins , which in turn promote the formation of oriented fibronectin at the cell surface ; oriented collagen is deposited between cells at the same stages . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The undifferentiated cells ( a ) were unable to spread on gelatin or laminin and grew as rounded colonies , although they were able to spread on fibronectin ( b ) showed reduced adhesion to laminin , but not fibronectin ( c ) expressed much reduced levels of beta 1 integrin , although levels of alpha 5 and alphaV were wild type ( d ) were less polarized with increased membrane protrusions compared with a vinculin ( / ) ES cell mutant ( e ) were unable to assemble vinculin or paxillin containing focal adhesions or actin stress fibers on fibronectin , whereas vinculin ( / ) ES cells were able to assemble talin containing focal adhesions . ^^^ Interestingly , these differentiated talin ( / ) ES cells were able to spread and form focal adhesion like structures containing vinculin and paxillin on fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| However , when the fibronectin is covalently linked to the substrate , alpha ( 5 ) beta ( 1 ) integrin forms highly tyrosine phosphorylated , `` classical ' ' focal contacts containing high levels of paxillin and vinculin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Fibronectin also elicited actin reorganization , co localization of beta 1 integrin and vinculin , formation of focal adhesions and tyrosine phosphorylation of focal adhesion kinase in myocytes . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In explanted human hearts failing because of dilated cardiomyopathy ( ejection fraction < / =20 % ) , expression of alpha and beta tubulin , desmin , vinculin , fibronectin , and vimentin was determined by Northern and Western blot analysis and compared with normal myocardium from explants not used for transplantation . ^^^ Protein levels for alpha tubulin increased 2 . 6 fold ( P < 0 . 02 ) ; for beta tubulin , 1 . 2 fold ( P < 0 . 005 ) ; for desmin , 2 . 1 fold ( P < 0 . 001 ) ; for vinculin , 1 . 2 fold ( P < 0 . 005 ) ; for fibronectin , 2 . 9 fold ( P < 0 . 001 ) ; and for vimentin , 1 . 5 fold ( P < 0 . 005 ) . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Using static light scatter technique , 1 observed affinities of the order talin > filamin > F actin > alpha actinin > ( talin when bound to vinculin ) which were lower when integrin was complexed with fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Among the cytoskeletal and membrane glycoproteins , fibronectin , 1 CAM , laminin , occludin , vinculin and desmin were most prominent . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| METHODS : Components of the focal contact machinery were analyzed in both cell lines , including the extracellular matrix glycoproteins fibronectin , laminin , and vitronectin ; cytoskeleton associated elements alpha actinin , talin , and vinculin ; and receptors for extracellular matrix and alpha ( 2 ) , alpha ( 3 ) , alpha ( 5 ) , alpha ( 5 ) , beta ( 1 ) , and beta ( 3 ) integrins . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Cell culture experiments revealed that freshly plated MCs organize alpha 8 into focal contacts within one hour after attachment to fibronectin and vitronectin substrata , showing colocalization with focal contact proteins vinculin and talin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Extracellular matrix ( fibronectin , type 1 collagen , osteopontin ) and cytoskeletal protein ( actin , vinculin ) orientation reflected the cell layer organization . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The remaining three factors were representative of vascular smooth muscle : factor 3 , including alphaSM actin , SM alpha actinin , SM22alpha , EDa+ fibronectin , and thrombospondin 1 ; factor 4 , metavinculin and h caldesmon ; and factor 5 , smooth muscle myosin SM 1 and desmin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| When CHO cells are replated on fibronectin , affixin and ILK but not FA kinase and vinculin concentrate at the cell surface in blebs during the early stages of cell spreading , which will grow into membrane ruffles on lamellipodia . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| FAK , alpha actinin , and vinculin were not cleaved in either cell type plated on fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To study the distribution of cytoskeletal proteins ( actin , alpha actinin , vinculin , beta tubulin , keratin , vimentin , desmin ) , adhesion molecules for cell matrix interations ( very later antigens [ VLA 1 61 , beta 1 , beta 2 [ CD 18 ] , vitronectin receptor [ alphavbeta 3 ] , CD 11b ) , leukocyte adhesion molecules ( ICAM 1 ) and extracellular matrix proteins ( collagen 4 , fibronectin , laminin , vitronectin ) in human and rat kidneys by using a superior processing and immunohistochemical staining technique . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| We quantitatively determined the amounts of laminin , fibronectin , vitronectin , and thrombospondin secreted by these glioma cell lines in vitro , as well as the amount of each of the eight beta integrin subunits and the adhesion complex related molecules , including talin , vinculin , profilin , zyxin , alpha actinin , paxillin , and VASP . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| One hour after seeding , cells adhered and spread moderately on fibronectin with the development of defined actin stress fibers and focal adhesions compared to NVP and GBL , on which the cells were spread with poorly developed stress fibers and a perinuclear localization of vinculin . ^^^ At 3 h , cells continue to spread more on fibronectin and NVP than GBL , and the cells on fibronectin had well defined stress fibers terminating with sharp spikes of vinculin , typical of focal adhesions . ^^^ However , by 24h , cells were much more spread on all three surfaces with defined stress fibers and focal contacts although EC expression of vinculin and paxillin was moderate on GBL compared to fibronectin and NVP . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| After 24 and 48 h of clinorotation , the cells significantly decreased fT 3 and fT 4 secretion but up regulated the thyroid stimulating hormone receptor expression as well as the production of vimentin , vinculin , and extracellular matrix proteins ( collagen 1 and 3 , laminin , fibronectin , chondroitin sulfate ) compared with controls . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Endothelialisation was studied on Nitinol bands with human umbilical venous endothelial cells ( HUVEC ) by 3 ( 4 , 5 dimethylthiazole 2yl ) 2 , 5 triphenyl tetrazolium ( MTT ) assay and immnuofluorescence analysis of Ki 67 , vinculin , fibronectin and von Willebrand Factor . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Here , endothelial GM 7373 cells spread and organize focal adhesion plaques on immobilized FGF 2 , fibronectin ( FN ) , and vitronectin ( VN ) . alpha ( 5 ) beta ( 3 ) integrin , paxillin , focal adhesion kinase , vinculin and pp 60 ( src ) localize in cell substratum contact sites on FGF 2 , FN or VN . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The variations of expression of cell adhesion proteins are evidenced by indirect immune fluorescence method : actin from the cytoskeleton , vinculin from the focal adhesion complex , fibronectin and collagen 1 from the extracellular matrix . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| TGFbeta 1 induced epithelial mesenchymal transition of SiHa cells was based on morphological and biochemical criteria ; actin stress fiber formation , focal translocalization of integrin alphav , talin , and vinculin , fibronectin based matrix assembly at the cell periphery , and translocalization and down regulation of E cadherin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Relative content of myospecific proteins ( KRP kinase related protein , desmin ) , cytoskeletal proteins ( tubulin , vinculin , and myosin light chain kinase MLCK ) and extracellular matrix protein , fibronectin , was determined by immunoblotting . ^^^ RESULTS : In 2 hours after injection of 50 mg / kg of isoproterenol destruction of some cardiomyocytes , contracture of myofibrils , and mild edema of intercellular space occurred ; the content of KRP decreased by l 6 % , and that of tubulin , vinculin and fibronectin by 27 29 % . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The light and electronic microscopy , immunohistochemical determination of type 1 , 3 and 4 collagens and fibronectin using specific antibodies were implied for morphological study ; electrophoresis and immunoblotting were implied for the determination of the content of some proteins of cardiomyocytes ( KRP or telokin , desmin , tubulin and vinculin ) , and extracellular matrix ( fibronectin ) and vascular smooth muscle cells ( MLCK , myosin light chain kinase ) . ^^^ The content of tubulin , fibronectin , MLCK and KRP was significantly decreased by 18 24 % , while contents of desmin and vinculin were changed insignificantly . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Quantitive immunoblotting was used to analyze the expression levels of cytoskeletal and extracellular matrix proteins such as desmin , tubulin , vinculin , fibronectin , kinase related protein ( KRP or telokin ) , and smooth muscle / nonmuscle myosin light chain kinase ( MLCK ) . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In situ hybridization for type 1 and 2 collagens , immunohistochemical analysis for fibronectin , alpha 5 and beta 1 integrins , paxillin , and vinculin , and cytochemical staining for actin were used to demonstrate the phenotypic change of chondrocytes . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The relative content of several myospecific proteins ( KRP kinase related protein , desmin ) , cytoskeletal proteins ( tubulin , vinculin , myosin light chain kinase MLCK ) and extracellular matrix protein fibronectin was determined by immunoblotting . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| DEP 1 expressing cells had fewer actin containing microfilament bundles , reduced vinculin and paxillin containing adhesion plaques , and were defective in interactions with fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| In addition , expression of the cell adhesion molecules E selectin , cadherin 5 and PECAM , as well as of the adhesion associated proteins fibronectin and vinculin ( focal adhesions ) , was determined by immunocytochemistry and western blotting . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Compared with WT vinculin , expression of this mutant in vinculin null cells results in diminished lamellipodial protrusion and spreading on fibronectin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Cells that spread well on fibronectin and PVAm [ Pep ( 100 % ) ] had sharp spikes of vinculin localized at the termination point of actin stress fibers . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| When AdCC cells were plated on collagen , the surface level of uPAR was increased , and numerous focal adhesions consisting of uPAR , vinculin , and paxillin were assembled ; whereas collagen stimulated SCC cell counterparts or AdCC cells plated on other types of ECM , such as fibronectin , failed to assemble such definite focal adhesions . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemistry of centrifuged samples showed an increase in number ( up to 160 % increase ) and thickness ( up to 49 % increase ) of actin fibers , a decrease in intensity of fibronectin fluorescence ( 18 23 % decrease ) and an increase in number of vinculin bulbs ( 202 374 % increase in number of vinculin bulbs / area ) . ^^^ While hypergravity exposure did not alter the number of attached osteoblasts , it did result in altered actin , fibronectin , and vinculin elements , changing some aspects of osteoblast substrate adhesion . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The tissues were subsequently sectioned and immunoreacted using antibodies for vinculin , talin , fibronectin , and caveolin to determine their cellular distribution in these tissues under these conditions . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Coimmunoprecipitation experiments showed that cell adhesion on fibronectin enhances the vinexin vinculin interaction . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Based on these data , we hypothesize that fucoidan inhibits the adhesion of MDA MB 231 cells to fibronectin 1 ) by blocking the protein ' s heparin and cell binding domains , 2 ) by modulating the reorganization of the integrin alpha 5 subunit and 3 ) by down regulating the expression of vinculin . . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| We also examined the distribution of F actin , alpha actinin , and vinculin in explanted fragments by immunocytochemical methods noting particularly their distribution with respect to FN lines . ^^^ Vinculin and alpha actinin in peripheral anchoring filopodia and lamellipodia co localized with the terminations of F actin bundles and with FN lines , suggesting that the peripheral cells were the moving force for explant translocation . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| The synthetic peptides ( Gly Arg Gly Asp [ GRGD ] and Gly Arg Gly Asp Ser Pro [ GRGDSP ] ) containing the tripeptide Arg Gly Asp ( RGD ) , originally found to be responsible for the cell binding activity of FN , have been found to inhibit EC spreading and the redistribution of their cytoskeleton , including the formation of stress fibers and the localization of vinculin either on fg or on FN . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Immunofluorescent staining of MEC cultures with antibodies to the integrin beta 1 subunit demonstrated receptors on the basolateral surface at focal adhesion plaques that co localized with vinculin and with Fn positive matrix fibers . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Both motile cell types also displayed relatively low numbers of focal contacts and microfilament bundles and limited amounts of localized vinculin , alpha actinin , and endogenous FN . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Concomitantly , a dramatic clustering of alpha 5 beta 1 Fn receptors and of the focal contact associated proteins vinculin and talin occurred . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Actin is one of the main protein components of the plate ; other proteins identified are a FN binding protein previously reported as a `` putative ' ' FN receptor the actin binding proteins myosin 2 , myosin 1 , alpha actinin , vinculin , and tropomyosin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Assembly of native FN induced a rapid reorganization of actin into stress fibers and colocalization of alpha 5 beta 1 integrin , focal adhesion kinase ( FAK ) , vinculin , and paxillin to regions of cell matrix contact . alpha 5 beta 1 integrins and FAK are also clustered upon binding of FN delta III 1 7 to cells but actin reorganization and focal adhesion formation are delayed and appear to be dependent on the formation of FN delta III 1 7 fibrils . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| RPMC formed focal adhesions on FN in the presence of a regular glucose concentration ( 5 . 6 mmol / L ) ; however , tyrosine phosphorylation of FAK and p130Cas and formation of focal adhesions observed by FAK and vinculin staining were substantially inhibited by high glucose . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| Co immunoprecipitation experiments showed that a multimolecular signaling complex was formed by the amoebic FN receptor , FAK , paxillin , and vinculin . ^^^ |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UQS6 and P18206 |
Pubmed |
SVM Score :0.0 |
| NA |
|