| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| The latter protein , named xCT , showed a significant homology with those recently reported to mediate cationic or zwitterionic amino acid transport when co expressed with 4F2hc . ^^^ Thus xCT is a new member of a family of amino acid transporters that form heteromultimeric complex with 4F2hc , with a striking difference in substrate specificity . ^^^ The expression of system xc was highly regulated , and Northern blot analysis demonstrated that the expression of both 4F2hc and xCT was enhanced in macrophages stimulated by lipopolysaccharide or an electrophilic agent . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| The functional identity of the cloned human xCT was investigated by heterologous coexpression of the light chain with the heavy chain ( human 4F2hc ) in HeLa cells . ^^^ Coexpression of human xCT with 4F2hc in HeLa cells leads to the induction of cystine and glutamate uptake with characteristics similar to that of 10 ( c ) ( ) . ^^^ The activity of 10 ( c ) ( ) in ARPE 19 cells is upregulated by SNAP , and the process is associated with an increase in the expression of xCT with no detectable change in the expression of 4F2hc . ^^^ CONCLUSIONS : ARPE 19 cells express the cystine / glutamate transporter 10 ( c ) ( ) ( the light chain xCT and the heavy chain 4F2hc ) as is evident from functional and molecular studies . ^^^ NO upregulates this transport system and the process is associated with an increase in xCT mRNA but with no change in 4F2hc mRNA . . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| System 10 ( c ) ( ) is composed of two protein components , xCT and 4F2hc , and the expression of xCT was closely correlated with system 10 ( c ) ( ) activity . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| The protein mediating this transport is a disulfide linked heterodimer of a light chain named xCT and a heavy chain previously known as 4F2hc . ^^^ When functionally expressed in Xenopus oocytes together with the human 4F2hc , human xCT induced the transport activity whose characteristics are similar to those of system xc . ^^^ Northern blot analysis demonstrated that the expression of both xCT and 4F2hc was significantly enhanced by oxygen . ^^^ The results suggest that oxygen regulates the activity of system xc by modulating the expression of both xCT and 4F2hc mRNAs . . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| Therefore , we have constructed mammalian expression vectors for murine xCT and its accessory subunit 4F2hc and transfected them into HEK 293 cells . ^^^ Surprisingly , xCT transport activity in HEK 293 cells is not dependent on the co expression of the exogenous 4F2hc . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| The two heavy subunits known today ( HSHAT : rBAT and 4F2hc ) were identified in 1992 , and light subunits ( LSHAT : LAT 1 , LAT 2 , asc 1 , y+ LAT 1 , y+ LAT 2 , xCT and b ( o , + ) AT ) have been cloned in the last 2 years . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| In agreement with this , 137 kDa band was detected by either anti xCT or anti 4F2hc antibodies in the non reducing condition in western blots , whereas it shifted to 50 kDa or 90 kDa bands in the reducing condition , indicating the association of two proteins via disulfide bands . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| We have identified a new human complementary deoxyribonucleic acid ( cDNA ) , for the xc amino acid transporter ( HGMW approved name SLC7A11 ; also known as human xCT ) , that , when co expressed with the heavy chain of surface antigen 4F2 ( 4F2hc , also termed CD 98 ) , but not with rBAT , ( related to the bo , + amino acid transporter ) , induces system xc transport activity in Xenopus oocytes . ^^^ Human xCT is the seventh human member of the family of amino acid transporters that are subunits of 4F2hc or rBAT and , inview of its amino acid sequence identity ( 89 % ) with mouse xCT , is most probably the human orthologue thereof . ^^^ The amino acid transport activity induced by the co expression of human 4F2hc and xCT in Xenopus oocytes was sodium independent and specific for L cystine , L glutamate and L aspartate . ^^^ When human xCT was co expressed with 4F2hc , the former localised to the oocyte plasma membrane . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| Acidic amino acid transporters , GLT 1 and system xc , which consists of xCT and 4F2hc , were expressed in all TR ASTs by RT PCR . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| The mRNA levels of system 10 ( ) ( c ) , which consists of xCT and 4F2hc , were determined by quantitative real time RT PCR analysis with specific primers . ^^^ RESULTS : The xCT and 4F2hc mRNAs were expressed in TR iBRB 2 cells . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| The mRNA levels of L cystine / L Glu exchanger , system 10 ( c ) ( ) , which consists of xCT and 4F2hc , were determined by quantitative real time reverse transcription polymerase chain reaction analysis . ^^^ The xCT and 4F2hc mRNAs were expressed in MBEC 4 cells and , following diethyl maleate ( DEM ) treatment , the xCT mRNA level and L cystine uptake in MBEC 4 cells were enhanced in parallel with an increase in DEM concentration ( up to 500 microM ) . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| The recently cloned transporter xCT forms a heteromultimeric complex with the heavy chain of 4F2 cell surface antigen ( 4F2hc / CD98 ) . ^^^ DEM caused time ( 3 24 h ) and concentration ( 25 100 microM ) dependent increases in L cystine transport , with GSH depleted by 50 % after 6 h and restored to basal values after 24 h . xCT mRNA levels increased after 4 h DEM treatment with negligible changes detected for 4F2hc mRNA . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| Transport system 10 ( c ) ( ) is a member of plasma membrane heterodimeric amino acid transporters and consists of two protein components , xCT and 4F2hc . ^^^ Intracellular GSH content was much higher in A2780DDP cells but it fell drastically in the presence of excess glutamate , which inhibited the cystine uptake competitively . xCT and 4F2hc mRNAs were definitely expressed in A2780DDP cells , but far less in A 2780 cells . ^^^ Expression of system 10 ( c ) ( ) activity by transfection with cDNAs for xCT and 4F2hc made A 2780 cells more resistant to CDDP . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| The expression of the activity of cystine / glutamate exchange transporter , designated system 10 ( c ) ( ) , requires two components , xCT and 4F2 heavy chain ( 4F2hc ) in Xenopus oocytes . rBAT ( related to b ( 0 , + ) amino acid transporter ) has a significant homology to 4F2hc and is known to be located in the apical membrane of epithelial cells . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| We found that TR MUL 5 cells exhibited [ ( 14 ) C ] L cystine uptake activity and expressed xCT and 4F2hc , which involve system 10 ( c ) ( ) . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| METHODS : The transport function of xc was measured as glutamate uptake in the absence of Na+ . mRNA levels for xCT and 4F2hc , the two subunits of system xc , were monitored by RT PCR and Northern blot and protein levels by Western blot . ^^^ The expression pattern of xCT and 4F2hc in the mouse retina was analyzed by immunofluorescence . ^^^ The transport function of xc was upregulated , and this increase was accompanied by increases in the levels of mRNAs for xCT and 4F2hc and in corresponding protein levels . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : System 10 ( ) ( c ) is a heterodimeric transporter , comprised of a light chain , xCT , and heavy chain , 4F2hc , which mediates the sodium independent exchange of cystine and glutamate at the plasma membrane . ^^^ In the current study we tested the hypothesis that stable transfection of Madin Darby canine kidney ( MDCK ) cells with human xCT and 4F2hc results in the expression of functional system 10 ( ) ( c ) . ^^^ METHODS : MDCK cells were transfected stably with human clones for xCT and 4F2hc . ^^^ Analyses of time and temperature dependence , saturation kinetics , and substrate specificity of l cystine and l glutamate transport were carried out in control and xCT 4F2hc transfected MDCK cells . ^^^ We also measured the uptake of l cystine in Xenopus oocytes expressing human xCT and / or 4F2hc or xCT and / or rBAT ( a heavy chain homologous to 4F2hc ) . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| SLC7A11 ( xCT ) , together with SLC3A2 ( 4F2hc ) , encodes the heterodimeric amino acid transport system 10 ( c ) , which mediates cystine glutamate exchange and thereby regulates intracellular glutathione levels . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| System 10 ( c ) ( ) , one of the main transporters responsible for central nervous system cystine transport , is comprised of two subunits , xCT and 4F2hc . ^^^ However , the distribution of xCT and 4F2hc within the brain and other areas has not been described . ^^^ Using specific antibodies , both xCT and 4F2hc were localized predominantly to neurons in the mouse and human brain , but some glial cells were labeled as well . ^^^ Border areas between the brain proper and periphery including the vascular endothelial cells , ependymal cells , choroid plexus , and leptomeninges were also highly positive for the system 10 ( c ) ( ) components . xCT and 4F2hc are also present at the brush border membranes in the kidney and duodenum . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| Expression of mRNA for xCT and 4F2hc subunits , which are components of the cystine / Glu antiporter , was seen in both cultured mouse metatarsals and rat costal chondrocytes . ^^^ |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UPY5 and P08195 |
Pubmed |
SVM Score :0.0 |
| NA |
|