| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| Furthermore , maelstrom mutant ovaries show mislocalization of two proteins involved in the microRNA and / or RNAi pathways , Dicer and Argonaute 2 , suggesting a potential connection between nuage and the microRNA pathway . . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| Biochemical analysis has revealed that dFMR 1 forms a complex that includes ribosomal proteins and , surprisingly , Argonaute 2 ( AGO 2 ) , an essential component of the RNA induced silencing complex ( RISC ) that mediates RNA interference ( RNAi ) in Drosophila . dFMR 1 also associates with Dicer , another essential processing enzyme of the RNAi pathway . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| Biochemical analysis of TRBP containing complexes revealed the association of Dicer TRBP with Argonaute 2 ( Ago 2 ) , the catalytic engine of RISC . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| PAZ PIWI domain ( PPD ) proteins , together with the RNA cleavage products of Dicer , form ribonucleoprotein complexes called RNA induced silencing complexes ( RISCs ) . ^^^ The PAZ domains of PPD and Dicer proteins were originally thought to mediate binding between PPD proteins and Dicer , although no evidence exists to support this theory . ^^^ Here we show that PAZ domains are not required for PPD protein Dicer interactions . ^^^ Rather , a subregion of the PIWI domain in PPD proteins , the PIWI box , binds directly to the Dicer RNase 3 domain . ^^^ Stable binding between PPD proteins and Dicer was dependent on the activity of Hsp 90 . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| PAZ and PIWI domain ( PPD ) proteins , also involved in RNAi and miRNA function , are the best characterized proteins known to interact with Dicer . ^^^ In this chapter we describe overexpression and purification of recombinant human Dicer , its biochemical properties , and mapping of domains responsible for Dicer PPD protein interactions . . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| PPD ( PAZ Piwi domain ) proteins and the Dicer family have been the subjects of intense study over the last 6 years . ^^^ However , over the last 2 years , great strides have been made towards elucidating how PPD proteins and Dicer regulate gene silencing at the pre and post transcriptional levels . ^^^ In the present review , we summarize the well known roles of these two classes of proteins in gene silencing pathways , as well as explore the evidence for novel roles of PPD and Dicer proteins . . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| We show here that RNAi in a hemocyte cell line of Anopheles gambiae , the principal malaria vector in Africa , requires expression of dicer 2 , Ago 2 and Ago 3 proteins . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| An RNAi based screen performed in Drosophila S 2 cells has revealed that Dicer 1 , Argonaute 1 ( Ago 1 ) and Ago 2 , components involved in microRNA ( miRNA ) processing and function , are required for the rapid decay of mRNA containing AREs of tumor necrosis factor alpha . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| Several known Ago 1 and / or Ago 2 associated proteins including Dicer were identified , but also two novel factors , the putative RNA helicase MOV 10 , and the RNA recognition motif ( RRM ) containing protein TNRC6B / KIAA1093 . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| The Dicer nuclease produces mature miRNAs and siRNAs from pre miRNAs and double stranded RNA ( dsRNA ) , respectively , and associates with Ago 2 . ^^^ Here , we studied the assembly of human RISC by presenting pre miRNA to immunopurified complexes that contain Ago 2 , Dicer , and TRBP . ^^^ Our findings demonstrate that , in humans , a miRNA loading complex ( miRLC ) is formed by Ago 2 and Dicer prior to their encounter with pre miRNA . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| Finally , we identified high frequency copy number abnormalities of Dicer 1 , Argonaute 2 , and other miRNA associated genes in breast and ovarian cancer as well as melanoma . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| The degradation did not result from the Ago 2 mediated endonucleolytic cleavage but it was Dicer and Ago 2 dependent . ^^^ The 3 ' untranslated region sequences of a few hundred transcripts that were commonly up regulated upon Ago 2 and Dicer knock downs showed a significant enrichment of putative miRNA binding sites . ^^^ The up regulation upon Ago 2 and Dicer knock downs was moderate and we found no evidence , at the mRNA level , for activation of silenced genes . ^^^ |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UKV8 and Q9UPY3 |
Pubmed |
SVM Score :0.0 |
| NA |
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