| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| This indicates that 1 ( closed ) complexes between Ad 2 DNA and RNA polymerase are rapidly inactivated by poly 1 , but that RS ( open ) complexes are relatively resistant . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| Steps in the processing of Ad 2 mRNA : poly ( A ) + nuclear sequences are conserved and poly ( A ) addition precedes splicing . ^^^ The conservation of poly ( A ) + Ad 2 nuclear RNA is about 4 times greater than of total Ad 2 nuclear RNA , approaching 100 % conservation of poly ( A ) + nuclear sequences . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| Each poly ( A ) containing cytoplasmic AD 2 MRNA contains at its 5 ' terminus the general structure m 7 GpppN1 pN2p or m 7 GpppN1mpN2mpNp as well as an average of 4 m6A and 0 . 5 1 m5C residues per molecule . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| Using the 3 ' end of the L 1 mRNA family of adenovirus 2 ( Ad 2 ) as a model system , we investigated transcription elongation following a poly ( A ) signal in a cell free system . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| Attenuation at the same site occurred when transcription of the core gene was directed by the Ad 2 major late promoter ( MLP ) and when the poly ( A ) signal , which precedes the attenuation site , was mutated from TATAAA to TAGAAA . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| The cDNAs were synthesized from poly ( A ) + mRNA isolated from HeLa cells early during Ad 2 infection . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| S 1 hybrid analyses located 3 ' ends of large poly ( A ) + nuclear RNA at 39 , 50 and 61 . 5 on the Ad 2 map . ^^^ The possible significance of these poly ( A ) RNAs to the transcriptional programme of Ad 2 is discussed . . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| The cytoplasmic , poly ( A ) containing RNA species complementary to all regions of the adenovirus type 2 ( Ad 2 ) genome expressed before the onset of viral DNA synthesis have been examined by `` Northern ' ' blotting . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| All the 13 SmaI fragments of Ad 2 DNA were separately inserted into the PstI site of the pBR 322 vector after addition of homopolymeric poly ( dG ) tails to the fragments and poly ( dC ) tails to the linearized plasmid . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| Approximately 10 % of the RNA labeled in vitro contained poly ( A ) , and the length of poly ( A ) was very similar to that of nuclear RNA isolated from Ad 2 infected cells . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| SV 40 and adenovirus 2 ( Ad 2 ) recombinant plasmids containing long segments of poly ( dC dG ) cloned adjacent to transcription control regions were methylated in vitro with Hbal methylase and transcribed in a soluble in vitro system . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| The interaction of Adenovirus 2 DNA and human placental RNA polymerase 2 in vitro satisfies criteria that suggest that at least some fraction of our purified polymerase preparations corresponds to prokaryotic holoenzyme and is able to initiate transcription at `` true ' ' promoters : ( 1 ) The purified enzyme forms highly stable complexes at specific sites on Ad 2 DNA ; Kass = 1 2 10 10 ( 12 ) M 1 . ( 2 ) Transcription of Ad 2 DNA from pre formed complexes with human RNA polymerase 2 is resistant to poly 1 . ( 3 ) Many of the stable binding sites correspond to Ad 2 promoters known to be active in vivo . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| Splicing of newly formed nuclear RNA transcripts has been demonstrated during adenovirus type 2 ( Ad 2 ) mRNA formation in HeLa cells in the presence of `` cordycepin , ' ' 3 ' deoxyadenosine , a drug that stops poly ( A ) addition to nuclear RNA . ^^^ The nuclear RNA from the 3 ' dA treated cells did not contain poly ( A ) but did contain Ad 2 specific molecules approximately 200 to 250 bases shorter than the spliced mRNAs of the control infected cells . ^^^ In addition , the approximately 2 kb RNA from Ad 2 region E 2 was shown to have sequences that lie more than 3 . 5 kb apart on the DNA , suggesting that correct cutting and splicing of the primary transcript in the absence of poly ( A ) synthesis had occurred . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| The cytoplasmic poly ( A ) RNA from early stages of infection of HeLa cells by adenovirus 2 ( Ad 2 ) , was used to synthesize cDNA . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| AdRG1 . 3 , which carries RG with accompanying SV 40 poly A addition sequences within an E 3 deletion , and AdRG 4 , which has RG in the E 3 deletion but under the control of an exogenous Ad 2 major late promoter , both express higher levels of RG in permissive cell culture than did AdRG 1 and both elicit high levels of serum anti rabies antibodies by parenteral or oral routes in animals . ^^^ |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P57721 and Q9UKE5 |
Pubmed |
SVM Score :0.0 |
| NA |
|