| Therefore , the physiological relevant EIAV Gag Alix interaction can be functionally replaced by a Gag Vps 28 CTD fusion . ^^^ Because both Alix and Vps 28 CTD can directly recruit ESCRT 3 proteins , ESCRT 3 assembly coupled to Vps 4 action may therefore constitute the minimal budding machinery for EIAV release . . ^^^ |