| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| The anaphase promoting complex is composed of eight protein subunits , including BimE ( APC 1 ) , CDC 27 ( APC 3 ) , CDC 16 ( APC 6 ) , and CDC 23 ( APC 8 ) . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| TPR containing proteins encoded by the yeast genes CDC 16 , CDC 23 , and nuc2+ are required for progression through the G2 / M transition of the cell cycle . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| In contrast , we show , using cdc 16 , cdc 17 ( 36 degrees C ) , cdc 20 , cdc 23 , and nocodazole treatment , that cells that arrest in the G 2 stage complete nuclear division in the absence of protein synthesis . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Before chromosome separation , novel nuclear DNA movements were observed in cdc 13 , cdc 16 , and cdc 23 cells but not in wild type or cdc 20 cells . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Through the isolation of mutants , we have identified three essential yeast genes , CDC 16 , CDC 23 , and CSE 1 , which are required for proteolysis of the B type cyclin CLB 2 but not of other unstable proteins . cdc 23 1 mutants are defective in both entering and exiting anaphase . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Three genes , CDC 16 , CDC 23 , and CSE 1 have recently been shown to be required specifically for cyclin B proteolysis in yeast . ^^^ The ubiquitination activity was cell cycle regulated , required Clb 2 ' s destruction box , and was low if not absent in cdc 16 , cdc 23 , cdc 27 , and cse 1 mutants . ^^^ The Cdc 16 , Cdc 23 , and Cdc 27 proteins all contain several copies of the tetratricopeptide repeat and are subunits of a complex that is required for the onset of anaphase . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| In addition to CDC 27 , CDC 16 , and CDC 23 , APC contained a homolog of Aspergillus nidulans BIME , a protein essential for anaphase . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| When pheromone arrested cells of cdc 16 and cdc 23 mutants were shifted to the restrictive temperature , they underwent DNA replication in the presence of pheromone . ^^^ This suggests that accumulation of any one of the six B type cyclin proteins could account for the precocious replication of cdc 16 and cdc 23 mutants . . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Most of these mutants lie in the CDC 16 , CDC 23 , and CDC 27 genes , which have already been shown to play a role in cyclin proteolysis and encode components of a 20S complex ( called the cyclosome or anaphase promoting complex ) that ubiquitinates mitotic cyclins . ^^^ Cdc 26 associates in vivo with Doc 1 , Cdc 16 , Cdc 23 , and Cdc 27 . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Three of the eight subunits of the anaphase promoting complex ( CDC 16 , CDC 23 , and CDC 27 ) have been shown to be phosphorylated in M phase , and their phosphorylation is required for the anaphase promoting complex to be active as a ubiquitin ligase . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| TPR containing proteins include the anaphase promoting complex ( APC ) subunits cdc 16 , cdc 23 and cdc 27 , the NADPH oxidase subunit p 67 phox , hsp 90 binding immunophilins , transcription factors , the PKR protein kinase inhibitor , and peroxisomal and mitochondrial import proteins . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| In contrast , the cell cycle arrest caused by mutations that induce DNA damage ( cdc 13 ) , inactivate the cyclin proteolysis machinery ( cdc 16 and cdc 23 ) , or arrest cells in anaphase ( cdc 15 ) is independent of the spindle checkpoint . . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , we find that while the inactivation of APC subunits Cdc 16 , Cdc 23 or Cdc 27 results in stabilization of the mitotic cyclin Clb 2 in G 1 , the proteolytic destruction of Cdc 20 remains largely unaffected . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Mutating putative Cdc 28 phosphorylation sites in three components of the APC , Cdc 16 , Cdc 23 , and Cdc 27 , makes the APC resistant to phosphorylation both in vivo and in vitro . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Of these , 32 sites are clustered in parts of Apc 1 and the tetratricopeptide repeat ( TPR ) subunits Cdc 27 , Cdc 16 , Cdc 23 and Apc 7 . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| Seven core subunit homologs of APC / C ( APC 1 , APC 2 , APC 11 , CDC 16 , CDC 23 , CDC 27 , and DOC 1 ) were identified in the Trypanosoma brucei genome data base . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| WD 40 repeats of Cdc 20 , Cdh 1 , and Cdc 16 and tetratricopeptide repeats of Cdc 16 , Cdc 23 , and Cdc 27 , all components of the anaphase promoting complex , are also analyzed . ^^^ |
|
| Interacting proteins: Q9UJX2 and Q13042 |
Pubmed |
SVM Score :0.0 |
| We found that the largest subunit , Apc 1 , serves as a scaffold that associates independently with two separable subcomplexes , one that contains Apc 2 ( Cullin ) , Apc 11 ( RING ) , and Doc1 / Apc10 , and another that contains the three TPR subunits ( Cdc 27 , Cdc 16 , and Cdc 23 ) . ^^^ We found that the three TPR subunits display a sequential binding dependency , with Cdc 27 the most peripheral , Cdc 23 the most internal , and Cdc 16 between . ^^^ |
|