| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.53843448 |
| Human cell extracts possess the adenine DNA glycosylase activity of hMYH and can form protein DNA complexes with both A / G and A / 8 oxoG mismatches . hMYH in cell extracts was shown to be the primary binding protein for A / G and A / 8 oxoG containing DNA substrates by UV cross linking . 0.53843448^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Uracil DNA glycosylase ( UDG ; EC 3 . 2 . 2 . ) removes uracil from DNA to initiate DNA base excision repair . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Cloning and sequencing a human homolog ( hMYH ) of the Escherichia coli mutY gene whose function is required for the repair of oxidative DNA damage . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| By expressing the epitope tagged proteins in COS 7 cells , we examined subcellular localizations of gene products of human DNA glycosylases : hOGG 1 , hMYH and hNTH 1 . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Differential subcellular localization of human MutY homolog ( hMYH ) and the functional activity of adenine : 8 oxoguanine DNA glycosylase . ^^^ In vitro expressed hMYH showed adenine DNA glycosylase activity toward the A : GO substrate . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Identification of human MutY homolog ( hMYH ) as a repair enzyme for 2 hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria . ^^^ Thus , hMYH is likely to possess both adenine and 2 OH A DNA glycosylase activities . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| DNA glycosylase activity of mtMYH was inhibited by anti MutY antibodies but not by anti hMYH peptide antibodies . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Alteration of DNA base excision repair enzymes hMYH and hOGG 1 in hydrogen peroxide resistant transformed human breast cells . ^^^ The DNA binding and glycosylase activities of hMYH and hOGG 1 were measured in the extracts of the H ( 2 ) O ( 2 ) resistant cells . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Based on these observations , we further characterized expression and intracellular localization of 8 oxoG DNA glycosylase ( hOGG 1 ) and 2 OH A / adenine DNA glycosylase ( hMYH ) in human cells . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The human MutY homolog ( hMYH ) , a DNA glycosylase , removes adenines from these mismatches . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| It is generally accepted that the repair of these promutagenic base pairs in human cells is initiated by the MutY DNA glycosylase homolog ( hMYH ) . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| We performed the real time reverse transcription polymerase chain reaction assay to evaluate the relative mRNA expression of three oxidative damage repair genes , human MutM homolog ( hMMH ) , hMTH , and human MutY homolog ( hMYH ) , with beta actin and human O ( 6 ) methylguanine DNA methyltransferase ( hMGMT ) as the internal controls . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Recent studies have shown that human MutY homologue ( hMYH ) , human 8 oxo 7 , 8 dihydrodeoxyguanine ( 8 oxoG ) glycosylase ( hOGG 1 ) , and human MutS homologue 2 ( hMSH 2 ) are important DNA mismatch repair genes . ^^^ We hypothesized that ischemia reperfusion injury in spinal cord causes DNA damage manifested by 8 oxoG production and activates the DNA repair system involving hMYH , hOGG 1 , and hMSH 2 . ^^^ The expression and localization of DNA repair enzymes , such as hMYH , hOGG 1 , and hMSH 2 , were studied with Western blot analysis and immunohistochemical staining . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The human MutY homolog ( hMYH ) is a DNA glycosylase involved in the removal of adenines or 2 hydroxyadenines misincorporated with template guanines or 7 , 8 dihydro 8 oxodeoxyguanines . hMYH is associated in vivo with apurinic / apyrimidinic endonuclease ( APE 1 ) , proliferating cell nuclear antigen ( PCNA ) , and replication protein A ( RPA ) in HeLa nuclear extracts as shown by immunoprecipitation and Western blotting . ^^^ By using constructs containing different portions of hMYH fused to glutathione S transferase , we have demonstrated that the APE 1 binding site is at a region around amino acid residue 300 , that the PCNA binding activity is located at the C terminus , and that RPA binds to the N terminus of hMYH . ^^^ Human homolog of the MutY repair protein ( hMYH ) physically interacts with proteins involved in long patch DNA base excision repair . ^^^ However , binding of hMYH to DNA polymerases beta and delta was not detected . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Autoantibody to DNA excision repair enzyme hMYH in a patient with rheumatic disease . ^^^ YT 1 , the longest cDNA clone isolated , has sequence identity to hMYH , the human homologue of the Escherichia coli excision repair enzyme , DNA adenine glycosylase MutY . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| These results suggest that the equivalent hMYH variants may be significantly compromised in substrate targeting in vivo due to a decrease in binding to substrate DNA ; moreover , competition with other DNA binding proteins may further reduce the effective adenine glycosylase activity in vivo . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Characterization of the recombinant MutY homolog , an adenine DNA glycosylase , from yeast Schizosaccharomyces pombe . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| This is the first demonstration that a MutY homolog plays an important role in protecting cells against oxidative DNA damage in eukaryotes . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The MutY homolog ( MYH ) is responsible for removing adenines misincorporated on a template DNA strand containing G or 7 , 8 dihydro 8 oxoguanine ( 8 oxoG ) and thus preventing G : C to T : A mutations . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Recent studies have shown that 2 ohA is removed from DNA by a human MutY homolog , MYH protein , in vitro . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| To evaluate the antimutagenic role of a mammalian mutY homolog , namely the Mutyh gene , which encodes adenine DNA glycosylase excising adenine misincorporated opposite 8 oxoguanine in the template DNA , we generated MUTYH null mouse embryonic stem ( ES ) cells . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The evolutionarily conserved enzyme adenine DNA glycosylase ( called MutY in bacteria and hMYH in humans ) initiates repair of A * oxoG to C * G by removing the inappropriately paired adenine base from the DNA backbone . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The human MutY ( hMYH ) enzyme , an adenine specific DNA glycosylase , initiates repair at this mismatch . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Unlike these enzymes , hMYH removes intact A misincorporated opposite template 8 oxoguanine during DNA replication . ^^^ For cleavage of the N glycosidic bond , bifunctional DNA glycosylases ( hNTH 1 , hNEIL 1 , hNEIL 2 , and hOGG 1 ) use Lys or Pro for direct attack on sugar C 1 ' , whereas monofunctional DNA glycosylases ( hSMUG 1 and hMYH ) use an activated water molecule . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| These lesions can be enzymatically repaired mainly by human MutT homolog 1 ( hMTH 1 ) , human 8 oxo guanine DNA glycosylase ( hOGG 1 ) and human MutY homolog ( hMYH ) . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Lesion specific DNA repair enzymes include hOgg 1 , hMYH , hNTH and hMTH . ^^^ CONCLUSION : Increased expression of the DNA glycosylase repair enzyme hMYH in A 549 cells exposed to O 2 and IR leads to improvements in cell survival . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| MutY homolog ( MUTYH ) excises adenine opposite 8 oxoguanine ( 8 oxoG ) in DNA , thus preventing occurrence of G : C to T : A transversion . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Interaction of checkpoint proteins Hus1 / Rad1 / Rad9 with DNA base excision repair enzyme MutY homolog in fission yeast , Schizosaccharomyces pombe . ^^^ The DNA glycosylase MutY homolog ( MYH ) is responsible for removing adenines misincorporated opposite DNA strands containing guanine or 7 , 8 dihydro 8 oxoguanine by base excision repair thereby preventing G : C to T : A mutations . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The base excision repair DNA glycosylase MutY homolog ( MYH ) is responsible for removing adenines misincorporated into DNA opposite guanine or 7 , 8 dihydro 8 oxo guanine ( 8 oxoG ) , thereby preventing G : C to T : A mutations . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The base excision repair carried out by bacterial MutY DNA glycosylase and eukaryotic MutY homolog ( MYH ) is responsible for removing adenines misincorporated into DNA opposite G and 7 , 8 dihydro 8 oxo guanines ( 8 oxoG ) ; thereby preventing G : C to T : A mutations . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| It binds the DNA glycosylase MutY homolog , and stimulates DNA polymerase beta , flap endonuclease 1 , and DNA ligase 1 . 9 1 1 resembles proliferating cell nuclear antigen ( PCNA ) , which stimulates some of these same repair enzymes , and is loaded onto DNA in a similar manner . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Mammals also have such DNA glycosylases ; OGG 1 ( Ogg 1 ) is the functional counterpart of MutM , and MUTYH ( Mutyh ) is the MutY homologue . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Enzymes for the BER pathway , namely , 8 oxoG DNA glycosylase ( OGG 1 ) , 2 OH A / adenine DNA glycosylase ( MUTYH ) , and AP endonuclease ( APEX 2 ) are also located both in the mitochondria and in the nuclei , and the expression of mitochondrial OGG 1 is altered in patients with various neurodegenerative diseases . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| We herein review that MTH 1 , MUTYH and OGG 1 play important roles in mammalian cells avoiding an accumulation of oxidative DNA damage , both in the nuclear and mitochondrial genomes , thereby suppressing carcinogenesis and cell death . ^^^ MUTYH also possesses a 2 OH A DNA glycosylase activity for excising 2 OH A incorporated into the cellular genomes . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| A functional analysis of the DNA glycosylase activity of mouse MUTYH protein excising 2 hydroxyadenine opposite guanine in DNA . 2 hydroxy 2 deoxyadenosine triphosphate ( 2 OH dATP ) , generated by the oxidation of dATP , can be misincorporated by DNA polymerases opposite guanine in template DNA during DNA replication , thus causing spontaneous mutagenesis . ^^^ We demonstrated that mouse MUTYH ( mMUTYH ) has a DNA glycosylase activity excising not only adenine opposite 8 oxoguanine ( 8 oxoG ) but also 2 hydroxyadenine ( 2 OH A ) opposite guanine , using purified recombinant thioredoxin mMUTYH fusion protein . mMUTYH formed a stable complex with duplex oligonucleotides containing an adenine : 8 oxoG pair , but the binding of mMUTYH to oligonucleotides containing a 2 OH A : guanine pair was barely detectable , thus suggesting that mMUTYH recognizes and interacts with these two substrates in a different manner which may reflect the difference in the base excision repair process for each substrate . ^^^ Mutant mMUTYH with G365D amino acid substitution , corresponding to a G382D germline mutation of human MUTYH found in familial adenomatous polyposis patients , almost completely retained its DNA glycosylase activity excising adenine opposite 8 oxoG ; however , it possessed 1 . 5 % of the wild type activity excising 2 OH A opposite guanine . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Cells with pathogenic biallelic mutations in the human MUTYH gene are defective in DNA damage binding and repair . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| MAP tumours display a mutational signature of somatic guanine to thymine transversion mutations in the adenomatous polyposis coli and K ras genes , reflecting the normal role of MutYH in the base excision repair of adenines misincorporated opposite 7 , 8 dihydro 8 oxoguanine , a prevalent and stable product of oxidative damage to DNA . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| In humans , the functional homologues of MutM , MutY and MutT , i . e . , OGG 1 , MUTYH ( MYH ) and MTH 1 , contribute to the protection of genomic DNA from oxidative stress . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| We evaluated whether genetic variation in six BER pathway genes ( XRCC 1 , ADPRT , APEX 1 , OGG 1 , LIG 3 , and MUTYH ) is associated with breast cancer risk in two large population based case control studies in the United States ( 3 , 368 cases and 2 , 880 controls ) and Poland ( 1 , 995 cases and 2 , 296 controls ) . ^^^ S . study participants with mouthwash DNA , we found no significant overall association between breast cancer risk and XRCC 1 R280H and R194W , ADPRT V726W , APEX 1 D148E , OGG 1 S326C , LIG 3 R780H , or MUTYH 5 ' untranslated region . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Calf MYH is a DNA glycosylase that specifically removes mispaired adenines from A / G , A / 7 , 8 dihydro 8 oxodeoxyguanine ( 8 oxoG or GO ) , and A / C mismatches ( mismatches indicated by slashes ) . ^^^ The eukaryotic MYH may be involved in the major repair of both replication errors and oxidative damage to DNA , the same functions as those of the E . coli MutY protein . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Likewise , we provide the first evidence for elevation of MYH , the mammalian homolog of the Escherichia coli MutY DNA glycosylase , in mammalian cells . ^^^ Recently , the human MYH was implicated in repair of oxidative DNA damage and shown to carry a mitochondrial localization sequence . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Adenine excisional repair function of MYH protein on the adenine : 8 hydroxyguanine base pair in double stranded DNA . ^^^ Adenine paired with 8 hydroxyguanine ( oh ( 8 ) G ) , a major component of oxidative DNA damage , is excised by MYH base excision repair protein in human cells . ^^^ Since repair activity of MYH protein on an A : G mismatch has also been reported , we compared the repair activity of His ( 6 ) tagged MYH proteins , expressed in Spodoptera frugiperda Sf 21 cells , on A : oh ( 8 ) G and A : G mismatches by DNA cleavage assay and gel mobility shift assay . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| We observed increased susceptibility to spontaneous carcinogenesis in MTH 1 null mice , which exhibit an increased occurrence of A : T > C : G and G : C > T : A transversion mutations . 8 Oxoguanine ( 8 oxoG ) DNA glycosylase , encoded by the OGG 1 gene , and adenine DNA glycosylase , encoded by the MUTYH gene , are responsible for the suppression of G : C to T : A transversions caused by the accumulation of 8 oxoG in the genome . ^^^ MUTYH deficiency may also increase G : C to T : A transversions through the misincorporation of 2 OH dATP , especially in the intestinal tract , since MUTYH can excise 2 hydroxyadenine opposite guanine in genomic DNA and the repair activity is selectively impaired by a mutation found in patients with autosomal recessive colorectal adenomatous polyposis . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The candidate genes belong to different DNA repair pathways : base excision repair ( OGG 1 , LIG 3 , APEX , POLB , XRCC 1 , PCNA , and MUTYH ) , nucleotide excision repair ( ERCC 1 , ERCC 2 , ERCC 4 , and ERCC 5 ) , double strand breaks repair ( XRCC 2 , XRCC 3 , and XRCC 9 ) , and reversion repair ( MGMT ) genes . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Six MYH genes were located within a 500 kilobase segment of human DNA . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Two DNA N glycosylases among those so far cloned from human , UNG 2 and MYH , are found to have the same PCNA binding motif . ^^^ Major substrates of these enzymes , a uracil opposite an adenine for UNG 2 and an adenine opposite an 8 oxoguanine for MYH , are formed during DNA replication . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| MYH , a mammalian homolog of Escherichia coli MutY , is a DNA glycosylase responsible for initiating base excision repair of such a mispair by excising the adenine opposite 8 oxoG . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Hypoxia induces mitochondrial DNA damage and stimulates expression of a DNA repair enzyme , the Escherichia coli MutY DNA glycosylase homolog ( MYH ) , in vivo , in the rat brain . ^^^ The enzyme is a homolog of the Escherichia coli MutY DNA glycosylase ( MYH ) , which excises adenine residues misincorporated opposite the oxidized base , oh8dG . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Enhanced activity of adenine DNA glycosylase ( Myh ) by apurinic / apyrimidinic endonuclease ( Ape 1 ) in mammalian base excision repair of an A / GO mismatch . ^^^ In this study we report stimulation of mammalian adenine DNA glycosylase activity by apurinic / apyrimidinic ( AP ) endonuclease using murine homolog of MutY ( Myh ) and human AP endonuclease ( Ape 1 ) , which shares 94 % amino acid identity with its murine homolog Apex . ^^^ It also appears that Ape 1 stimulates Myh glycosylase activity by increasing formation of the Myh DNA complex . ^^^ Consequently , Ape 1 preserves the Myh preference for A / GO over A / G and improves overall glycosylase efficiency . ^^^ After removal of adenine by the Myh glycosylase activity , intact AP DNA remains due to lack of an efficient Myh AP lyase activity . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| To elucidate the involvement of 8 hydroxyguanine ( oh ( 8 ) G ) repair genes in human lung carcinogenesis , 47 lung cancer cell lines and 55 primary lung cancers were examined for somatic mutations and genetic polymorphisms in all coding exons of the MYH and APEX genes , and exon 8 of the OGG 1 gene by polymerase chain reaction single strand conformation polymorphism analysis . ^^^ Differences in the oh ( 8 ) G repair activities of MYH , APEX and OGG 1 proteins due to somatic mutations and SNPs can be involved in human carcinogenesis . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Adenine paired with 8 hydroxyguanine , a major oxidatively damaged DNA lesion , is excised by mutY homologue ( MYH ) base excision repair protein in human cells . ^^^ Since genetic polymorphisms of DNA repair genes associated with the activities and the expression levels of their products may modulate cancer susceptibility of individuals , we investigated the effect of a single nucleotide polymorphism ( SNP ) in the MYH gene on the difference in the expression levels of its products . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The Myh DNA glycosylase removes mismatched adenines incorporated opposite 8 oxoG during replication . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Rat MYH , a glycosylase for repair of oxidatively damaged DNA , has brain specific isoforms that localize to neuronal mitochondria . ^^^ We show that the Escherichia coli MutY DNA glycosylase homolog ( MYH ) in rat ( rMYH ) involved in repair of oxidative damage is abundantly expressed in the rat brain , with isoforms that are exclusive to brain tissue . ^^^ To assess involvement of MYH in the neuronal response to oxidative DNA damage , we used a rat model of respiratory hypoxia , in which acutely reduced blood oxygenation leads to generation of superoxide , and formation and subsequent removal of 8 hydroxy 2 ' deoxyguanosine ( 8OHdG ) . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Inherited defects in the DNA glycosylase MYH cause multiple colorectal adenoma and carcinoma . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| In this study , we assessed the abilities of OGG 1 , MYH and APE 1 proteins , which are components of a base excision repair pathway , to suppress G : C to T : A transversions caused by 8OHG or BPDE by a bacterial suppressor tRNA ( supF ) forward mutation assay using a shuttle plasmid , pMY 189 . ^^^ Both the mutation frequency of the 8OHG containing plasmid in NCI H 1299 cells and the occurrence of G : C to T : A transversions at position 159 in the supF gene were significantly reduced by overexpression of OGG 1 and MYH proteins , but not by that of APE 1 protein . ^^^ In contrast , neither mutation frequency nor the occurrence of G : C to T : A transversion of the BPDE treated plasmid was reduced by overexpression of OGG 1 , MYH and APE 1 proteins . ^^^ Suppressive activities of OGG 1 and MYH proteins against G : C to T : A mutations caused by 8 hydroxyguanine but not by benzo [ a ] pyrene diol epoxide in human cells in vivo . 8 Hydroxyguanine ( 8OHG ) , an oxidatively damaged base , and benzo [ a ] pyrene diol epoxide ( BPDE ) , a metabolite of benzo [ a ] pyrene found in cigarette smoke , are thought to be major causes for G : C to T : A transversions in DNA of human cells . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| This structure also suggests a rationale for the frequent occurrence in certain human cancers of a specific mutation in the related DNA repair protein MYH . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| However , it was recently discovered that biallelic mutations in the BER DNA glycosylase MYH lead to an autosomal recessive syndrome of adenomatous colorectal polyposis and very high colorectal cancer risk . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The base excision repair gene MYH protects against damage to DNA from reactive oxygen species , which are commonly found in cigarette smoke . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Developmental changes in expression and subcellular localization of the DNA repair glycosylase , MYH , in the rat brain . ^^^ An enzyme involved in repair of replication errors generated on either normal or oxidatively damaged DNA templates , is the mammalian ortholog of the Escherichia coli MutY DNA glycosylase ( MYH ) . ^^^ Together , these findings suggest that in proliferating embryonic cells , MYH might be primarily involved in post replicative repair of nuclear DNA , whereas in post mitotic neurons , in the repair of mitochondrial DNA . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Expression of DNA repair protein : MYH , NTH 1 , and MTH 1 in colorectal cancer . ^^^ The DNA repair protein : MYH , NTH 1 and MTH 1 eliminate the oxidative DNA damage . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The MYH gene encodes a DNA glycosylase involved in the excision repair of adenines paired with 8 hydroxyguanines , a major component of oxidative DNA damage , and bi allelic germline MYH mutations have been reported to predispose individuals to multiple colorectal adenomas and carcinoma . ^^^ These results suggested that the ability to repair 8 hydroxyguanine in nuclear DNA may differ among Japanese individuals due to the splicing abnormality based on the MYH IVS 10 2A > G variant , and that the bi allelic IVS 10 2A > G variation may be responsible for the occurrence of GC . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| METHODS : The analysis was performed in 14 unrelated patients by using the protein truncation test for APC and genomic DNA sequencing for MYH . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Quantification of cardiomyocyte concentrations of DNA damage by products ( 8 oxoG ) and mismatch repair enzymes ( MYH , OGG 1 , MSH 2 ) reflects the severity of OS . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The mammalian homolog of the bacterial DNA mismatch repair enzyme MutY ( MYH ) removes A * 8 oxoG from nuclear and mtDNA , reduces 8 oxoG accumulation , and restores genomic stability after ROS exposure . ^^^ To evaluate this hypothesis , we compared mtH2O2 production , MYH expression , oxidative DNA damage , and hepatocyte death in healthy mice and different mouse models of fatty liver disease . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Accumulation of the oxidative base lesion 8 hydroxyguanine in DNA of tumor prone mice defective in both the Myh and Ogg 1 DNA glycosylases . ^^^ The OGG 1 and MYH DNA glycosylases prevent the accumulation of DNA 8 hydroxyguanine . ^^^ In Myh ( / ) mice , there was no time dependent accumulation of DNA 8 hydroxyguanine in brain , small intestine , lung , spleen , or kidney . ^^^ Inactivation of both MYH and OGG 1 caused an age associated accumulation of DNA 8 hydroxyguanine in lung and small intestine . ^^^ The effects of abrogated OGG 1 and MYH on hepatic DNA 8 hydroxyguanine levels were additive . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Furthermore , these 2 MYH mutations were not found among patients whose tumors showed the presence of defective DNA mismatch repair ( n = 62 ) . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The DNA damage repair genes , mutY and mutM , prevent G to T mutations caused by reactive oxygen species in Escherichia coli , but it has remained debatable whether deficiencies in their mammalian counterparts , Myh and Ogg 1 , are directly involved in tumorigenesis . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| We have screened a set of 95 sporadic gastric cancers for mutations and allele loss of the DNA glycosylase MYH gene , which excises adenine misincorporated opposite unrepaired 8 oxoG . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The mammalian homolog of the Escherichia coli MutY DNA glycosylase ( MYH ) cleaves adenine residues paired with either oxidized or non modified guanines . ^^^ MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Mispaired dA is removed by post replicative base excision repair ( BER ) initiated by adenine DNA glycosylase , MYH , creating an apurinic ( AP ) site . ^^^ These results indicate that human DNA ligases discriminate dC from dA and that MYH initiated short patch BER is futile and hence this BER must proceed to long patch repair , even if it is initiated as short patch repair , through strand displacement synthesis from the ligation resistant dC terminus to generate the OGG 1 substrate , dC : 8 oxo dG pair . . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| MAP is caused by biallelic inactivating mutations of the MYH gene , a component of the base excision repair ( BER ) machinery , whose dysfunction leads to an increase in the rate of G > T transversions following DNA oxidative damage . ^^^ |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The MutY human homologue ( MYH ) gene encodes a member of the base excision repair pathway that is involved in repairing oxidative damage to DNA . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| We have determined the complete DNA nucleotide sequence of the carp Cyprinus carpio fast skeletal myosin heavy chain ( MYH ) gene . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| MYH testing thus can be considered for patients who meet clinical criteria for HNPCC in the absence of DNA mismatch repair gene mutations . . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Oxidative stress mediates a DNA mismatch lesion ( 7 , 8 dihydro 8 oxoguanine [ 8 oxo G ] ) , which is repaired by the enzymes MutY homologue ( MYH ) , 8 oxo G glycosylase ( OGG 1 ) , and MutS homologue 2 ( MSH 2 ) . ^^^ The DNA repair enzymes MYH , OGG 1 , and MSH 2 were also markedly decreased in the static preservation versus continuous hypothermic perfusion groups . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| The first section will address attenuated familial adenomatous polyposis and a newly recognized type of autosomal recessive adenomatous polyposis associated with the DNA repair gene MYH . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Seventy nine unrelated APC mutation negative Swiss patients with either classical ( n=18 ) or attenuated ( n=61 ) polyposis were screened for germline mutations in MYH by dHPLC and direct genomic DNA sequencing . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the DNA damage marker , 8oxoG ( 8 oxo 7 , 8 dihydroguanidine ) , was increased , whereas the DNA repair marker , MYH glycosylase , was decreased in old ob / ob and db / db compared with old WT mice . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| MYH DNA clones were also isolated from the slow muscle of adult carp acclimated to 10 degrees C ( MYHS 10 ) and 30 degrees C ( MYHS 30 ) . ^^^ A MYH DNA clone was isolated from the cardiac muscle of 10 degrees C acclimated adult fish ( MYHcard ) . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Two types of the fast skeletal myosin heavy chain ( MYH ) genes were cloned from a genomic DNA library of carp ( Cyprinus carpio L . ) and named MYH 10 and MYH 30 , which showed the sequence similarity to the MYH cDNAs predominantly expressed in carp acclimated to 10 and 30 degrees C , respectively . ^^^ The DNA fragment of 921 to 824 bp in MYH 10 and MEF 2 binding site in MYH 30 interacted with nuclear proteins extracted from carp fast skeletal muscle as revealed by electrophoretic mobility shift assay . ^^^ The signal intensity of a complex formed between the DNA fragment of MYH 10 and nuclear extracts from the 10 degrees C acclimated carp were higher than those with extracts from the 30 degrees C acclimated fish . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Here , we analyzed MYH and APC mutant polyps by combining laser capture microdissection , isothermal genomic DNA amplification , and array comparative genomic hybridization . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| MYH was analyzed in 50 cancer patients and 116 healthy controls by complete genomic DNA sequencing . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| Molecular genetic analysis of the MYH gene revealed 15 DNA variations ( 25 % ) including two patients identified as p . ^^^ |
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| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UIF7 and P27695 |
Pubmed |
SVM Score :0.0 |
| NA |
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