| Madm co immunoprecipitated with Mlf 1 and co localized in the cytoplasm . ^^^ In addition , Madm recruited a serine kinase , which phosphorylated both Madm and Mlf 1 including the RSXSXP motif . ^^^ In contrast to wild type Mlf 1 , the oncogenic fusion protein nucleophosmin ( NPM ) MLF 1 did not bind 14 3 3zeta , had altered Madm binding , and localized exclusively in the nucleus . ^^^ Ectopic expression of Madm in M 1 myeloid cells suppressed cytokine induced differentiation unlike Mlf 1 , which promotes maturation . ^^^ Because the Mlf 1 binding region of Madm and its own dimerization domain overlapped , the levels of Madm and Mlf 1 may affect complex formation and regulate differentiation . ^^^ |