| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| Here they investigate further ASIC1a in gut mechanoreceptors , and compare its influence with ASIC 2 and ASIC 3 . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| In bone , ASIC 2 and ASIC 3 were most abundant , while in chondrocytes it was ASIC 1 . ^^^ In human osteoblasts ASIC 1 , ASIC 2 , and ASIC 3 mRNAs were shown . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| The acid sensing ion channel ( ASIC ) subunits ASIC 1 , ASIC 2 , and ASIC 3 are members of the amiloride sensitive Na+ channel / degenerin family of ion channels . ^^^ A splice variant of ASIC 2 , ASIC2b , is not active on its own but modifies the properties of ASIC 3 . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| Single channel properties of recombinant acid sensitive ion channels formed by the subunits ASIC 2 and ASIC 3 from dorsal root ganglion neurons expressed in Xenopus oocytes . ^^^ Here , we have examined the single channel properties of channels formed by the subunits ASIC 2 and ASIC 3 expressed in Xenopus oocytes using outside out patches . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| RESULTS : In the Western blot , there was a significant three fold increase in the mean relative optical density of the ASIC 3 55 kDa band ( but not ASIC 1 or ASIC 2 ) in full thickness inflamed intestine , as well as in separated muscle and mucosal layers . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| We have raised specific antibodies for ASIC 1 , ASIC 2 , ASIC 3 , and ASIC 4 to examine the distribution of these proteins in neurons from dorsal root ganglia ( DRG ) and to determine their subcellular localization . ^^^ Immunohistochemical experiments and functional measurements of unitary currents from the ASICs with the patch clamp technique indicate that ASIC 1 localizes to the plasma membrane of small , medium , and large diameter cells , whereas ASIC 2 and ASIC 3 are preferentially in medium to large cells . ^^^ Neurons coexpressing ASIC 2 and ASIC 3 form predominantly heteromeric ASIC 2 3 channels . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| RT PCR of retinal RNA revealed the presence of alpha , beta , gamma , and delta ENaC as well as acid sensing ion channel ( ASIC ) 1 , ASIC 2 , ASIC 3 , and ASIC 4 . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| Acid sensing ion channels ASIC 2 and ASIC 3 do not contribute to mechanically activated currents in mammalian sensory neurones . ^^^ Amongst candidate transducer molecules are members of the acid sensing ion channel ( ASIC ) family ; nerve fibre recordings have shown ASIC 2 and ASIC 3 null mutants have aberrant responses to suprathreshold mechanical stimuli . ^^^ We cultured neurones from ASIC 2 and ASIC 3 null mutants and compared response properties with those of wild type controls . ^^^ No differences in current amplitude or kinetics were found between ASIC 2 and / or ASIC 3 null mutants and controls . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| Mammalian ASIC2a and ASIC 3 subunits co assemble into heteromeric proton gated channels sensitive to Gd3+ . ^^^ To investigate the subunit composition of native ASICs in peripheral and central neurons , we co injected human as well as rodent ASIC2a and ASIC 3 subunits in Xenopus oocytes . ^^^ The amplitudes of acid induced biphasic responses mediated by co expressed ASIC2a and ASIC 3 subunits were much larger ( as much as 20 fold ) than the currents mediated by the respective homomers , clearly indicating functional association . ^^^ The sensitivity to pH or amiloride of single versus co expressed ASIC subunits was not significantly different ; however , gadolinium ions inhibited ASIC 3 and ASIC2a+3 responses with much higher potency ( IC ( 50 ) approximately 40 microm ) than the ASIC2a response ( IC ( 50 ) > / =1 mm ) . ^^^ Biochemical interaction between ASIC2a and ASIC 3 subunits was demonstrated by co purification from transfected human embryonic kidney ( HEK 293 ) cells and Xenopus oocytes . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| ASIC2a , ASIC1a+2a , ASIC2a+3 ) , but not of homomeric ASIC1a and ASIC 3 . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| These modulatory effects were not observed with the corresponding homomeric human ASIC2A or ASIC 3 receptor subtypes . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| Here , we have examined the properties of unitary currents of homomeric rat ASIC1alpha , ASIC2a , and ASIC 3 expressed in Xenopus oocytes with the patch clamp technique . ^^^ However , ASIC1alpha exhibits three subconductance states , ASIC2a exhibits only one , and ASIC 3 none . ^^^ ASIC1alpha , ASIC2a , and ASIC 3 are activated by external protons with apparent pH ( 50 ) of 5 . 9 , 5 . 0 , and 5 . 4 , respectively . ^^^ Desensitization in the continual presence of protons is fast and complete in ASIC1alpha and ASIC 3 ( 2 . 0 and 4 . 5 s ( 1 ) , respectively ) but slow and only partial in ASIC2a ( 0 . 045 s ( 1 ) ) . ^^^ The response to external Ca ( 2+ ) also differs : micro M concentrations of extracellular Ca ( 2+ ) are necessary for proton gating of ASIC 3 ( EC ( 50 ) = 0 . 28 micro M ) , whereas ASIC1alpha and ASIC2a do not require Ca ( 2+ ) . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| APETx 2 reversibly inhibits rat ASIC 3 ( IC50=63 nM ) , without any effect on ASIC1a , ASIC1b , and ASIC2a . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| We show here that serine proteases modulate the function of ASIC1a and ASIC1b but not of ASIC2a and ASIC 3 . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| With ASICs expressed in Chinese hamster ovary cells , Zn2+ inhibits currents mediated by homomeric ASIC1a and heteromeric ASIC1a ASIC2a channels , without affecting currents mediated by homomeric ASIC1beta , ASIC2a , or ASIC 3 . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| In Chinese Hamster Ovary cells , redox modifying agents only affected the current mediated by homomeric ASIC1a , but not homomeric ASIC1b , ASIC2a , or ASIC 3 . ^^^ |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q16515 and Q9UHC3 |
Pubmed |
SVM Score :0.0 |
| NA |
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