Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.7585535
Following ligation of CD 2 on the surface of the Jurkat T cell line and human purified T cells , LAT was tyrosine phosphorylated and shown to associate in vivo with a number of other tyrosine phosphorylated proteins including PLCgamma 1 , Grb 2 , and SLP 76 . 0.7585535^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.63047457
On the other hand , the amino terminal SH 2 [ SH 2 ( N ) ] domain was not essential for reconstitution of RE / AP or IL 2 promoter dependent transcription but was required for the association of PLC gamma 1 with LAT , as well as the tyrosine phosphorylation of PLC gamma 1 itself , in activated P 98 cells . 0.63047457^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Phosphorylated LAT binds Grb 2 , PLC gamma 1 , and other signaling molecules . ^^^ After T cell activation , most tyrosine phosphorylated LAT molecules and a fraction of PLC gamma 1 and other signaling molecules are present in GEMs . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
In addition to Zap 70 , the 3BP2 SH 2 domain associated in vitro with LAT , Grb 2 , PLCgamma 1 , and Cbl from activated T cell lysates . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
LAT is required for TCR mediated activation of PLCgamma 1 and the Ras pathway . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
The results indicate a role for Shb as a link between the TCR and downstream signaling events involving LAT and PLC gamma 1 and resulting in the activation of transcription of the interleukin 2 gene . . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
The study of molecules such as LAT , SLP 76 , FYB , SKAP 55 and VAV have revealed multiple mechanisms with which to control the activation of downstream signaling pathways through RAS , PLC gamma 1 and ERK / MAPK . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
LAT ( linker for activation of T cells ) is an adaptor molecule , which following its phosphorylation associates with Grb 2 , Gads , PLC gamma 1 , and other signalling molecules . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Importantly , CD 3 induced responses , such as tyrosine phosphorylation of cellular substrates ( LAT , SLP 76 , and PLC gamma 1 ) , as well as calcium mobilization , which are defective in T cells expressing neither ZAP 70 nor Syk , are observed in Syk ( hi ) / ZAP 70 ( ) T cells . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Co immunoprecipitation experiments revealed a constitutive association of p 36 LAT with both FcgammaRI and FcgammaRIIa immunoprecipitates , and an activation induced association of LAT with PLCgamma 1 , Grb 2 , and the p 85 subunit of phosphatidylinositol 3 kinase . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Whereas tyrosine phosphorylation of Fc ( epsilon ) RI , Syk , and Vav was intact in LAT deficient BMMCs following Fc ( epsilon ) RI engagement , tyrosine phosphorylation of SLP 76 , PLC gamma 1 , and PLC gamma 2 and calcium mobilization were dramatically reduced . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
The kinetics of protein complex enhancement correlated with TCR / CD 3 induced tyrosine phosphorylation of PLCgamma 1 ; however , those PLCgamma 1 molecules in complex with hSos 2 were non phosphorylated after TCR / CD 3 stimulation , in contrast to the phosphorylated PLCgamma 1 associated with the linker for activation of T cells , LAT . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
However , phospholipase Cgamma 1 ( PLCgamma 1 ) and LAT were strikingly hypophosphorylated in CD 148 expressing cells following TCR stimulation , whereas the phosphorylation levels of Slp 76 and Itk were modestly reduced . ^^^ Based on these results , we propose that CD 148 negatively regulates TCR signaling by interfering with the phosphorylation and function of PLCgamma 1 and LAT . . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
By substituting these tyrosine residues in LAT with phenylalanine and by utilizing phosphorylated peptides derived from these sites , we mapped the tyrosine residues in LAT required for the direct interaction and activation of Vav , p85 / p110alpha and phospholipase Cgamma 1 ( PLCgamma 1 ) . ^^^ Furthermore , by expression of LAT mutants in LAT deficient T cells , we demonstrate that Tyr ( 191 ) and Tyr ( 171 ) are required for T cell activation and Tyr ( 132 ) is required for the activation of PLCgamma 1 and Ras signalling pathways . . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
In the Jurkat T cell line , the distal four tyrosines of LAT bind PLCgamma 1 , Grb 2 , and Gads . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Before activation , PLCgamma 1 and Gab 2 are not strongly membrane associated , LAT occurs in small membrane clusters separate from receptor , and PLCgamma 2 , that coprecipitates with LAT , occurs in clusters and along cytoskeletal cables . ^^^ PLCgamma 1 and another portion of p 85 associate preferentially with activated LAT . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Tyrosine phosphorylation of raft targeted PLCgamma 1 did not require Zap 70 or the interaction with the adapters Lat and Slp 76 , molecules that are necessary for TCR signaling . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Phosphorylation of LAT creates binding sites for the Src homology 2 ( SH 2 ) domains of other proteins , including PLC gamma 1 , Grb 2 , Gads , Grap , 3BP2 , and Shb , and indirectly binds SOS , c Cbl , Vav , SLP 76 , and Itk . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Moreover , the signal supplied by CD 28 does not lead to increased Zap 70 and Lat phosphorylation , but amplifies PLCgamma 1 activation and Ca ( 2+ ) response . ^^^ Our data suggest that CD 28 binding to B 7 contributes to setting the level of TCR induced phosphorylated Lat for recruiting signaling complexes , whereas the CD 28 signal boosts multiple pathways by facilitating PLCgamma 1 activation . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
T cell antigen receptor ( TCR ) induced activation of phospholipase C gamma 1 ( PLC gamma 1 ) and of nuclear factor of activated T cells , calcium influx , interleukin 2 production , and cell death were reduced or abrogated in T cells from LAT mutant mice . ^^^ These results identify a critical role for integrated PLC gamma 1 and Ras Erk signaling through LAT in T cell development and homeostasis . . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Upon T cell antigen receptor ( TCR / CD3 ) engagement , LAT is phosphorylated by protein tyrosine kinases ( PTK ) and binds to the adaptors Gads and Grb 2 , as well as to phospholipase Cgamma 1 ( PLCgamma 1 ) , thereby facilitating the recruitment of key signal transduction components to drive T cell activation . ^^^ The LAT tyrosine residues Y ( 132 ) , Y ( 171 ) , Y ( 191 ) , and Y ( 226 ) have been shown previously to be critical for binding to Gads , Grb 2 , and PLCgamma 1 . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
CD 2 cross linking strongly induced tyrosine phosphorylation of LAT , resulting in increased association with phosphatidylinositol 3 kinase ( PI 3 K ) and phospholipase C gamma 1 ( PLC gamma 1 ) . ^^^ In vitro binding studies using glutathione S transferase fusion proteins demonstrated that a large portion of the association between LAT and PI 3 K or PLC gamma 1 was mediated through their SH 2 domains in tyrosine phosphorylation dependent manner . ^^^ These results document that modulation of raft integrity by aggregation of NK cell activating receptors , which leads to the formation of complexes of LAT with PI 3 K and PLC gamma 1 , is essential for the NK cell lytic mechanisms . . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
New topographical data are integrated with existing knowledge of the biochemistry of FcepsilonRI signaling and of cell shape during signaling to implicate at least two distinct membrane domains in FcepsilonRI signaling . `` Primary signaling domains ' ' , also called osmiophilic patches , are recognized by their dark staining with osmium , adjacency to coated pits ( previously mapped to planar membrane between lamellae ) and by the characteristic presence of receptor , Syk and PLCgamma 2 , but not Lyn . `` Secondary signaling domains ' ' are characterized by the presence of large elliptical linker for activation of T cells ( LAT ) rafts and of PLCgamma 1 ( previously mapped to lamellae ) but not receptor . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Grb 2 , Gads , and PLC gamma 1 associated with LAT preferentially via different sets of tyrosine residues ; however , they failed to interact with LAT mutants containing only one tyrosine . ^^^ LAT mutants that were capable of binding Grb 2 and PLC gamma 1 could reconstitute T cell activation in LAT deficient cells and thymocyte development in LAT deficient mice . . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Furthermore , mice possessing mutations in signaling molecules upstream of PLC gamma 1 , such as Src homology 2 ( SH 2 ) domain containing phosphoprotein of 76 kDa ( SLP 76 ) , linker for activation of T cells ( LAT ) and Vav 1 , or in members of the nuclear factor for activated T cells ( NFAT ) family of transcription factors , which are downstream of PLC gamma 1 , have been found to have similar phenotypes to Tec family deficient mice , emphasizing the importance of this pathway in regulating T cell activation , differentiation and homeostasis . . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Z / 3 Cbl induces PLC gamma 1 activation in T lymphocytes via an alternate Lat and Slp 76 independent signaling mechanism . ^^^ The complete elimination of PLC gamma 1 phosphorylation required deleting the SH 3 domain binding region of 70Z / 3 Cbl , consistent with 70Z / 3 Cbl binding the PLC gamma 1 SH 3 domain . 70Z / 3 Cbl induced PLC gamma 1 phosphorylation required Zap 70 , as for the TCR , and the tyrosine kinase binding domain of 70Z / 3 Cbl , which binds Zap 70 , but did not require PLC gamma 1 binding to Lat , a crucial interaction in TCR induced PLC gamma 1 phosphorylation . ^^^ These results suggest that 70Z / 3 Cbl and PLC gamma 1 form a TCR , Lat and Slp 76 independent complex that leads to PLC gamma 1 phosphorylation and activation . . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Thr 155 phosphorylation reduces the ability of LAT to recruit PLCgamma 1 and SLP 76 , leading to attenuation of subsequent downstream events such as [ Ca2+ ] 1 mobilization and activation of the ERK pathway . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Crosslinked receptors and their signaling partners segregate during signaling to multiple , dynamic membrane domains , including a transient Fc ( epsilon ) RI Lyn domain and at least two other distinct domains , one characterized by the presence of receptor , Syk and multiple signaling proteins , but not Lyn ( primary signaling domains ) , and one characterized by the presence of LAT and PLCgamma 1 but not receptor ( secondary signaling domains ) . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Crosslinking of purified wild type nave CD 4 cells with anti CD 3 activated Lck and initiated the signaling cascade downstream of Lck , including phosphorylation of ZAP 70 , LAT , and PLC gamma 1 ; calcium flux ; and dephosphorylation and nuclear translocation of the nuclear factor of activated T cells ( NFAT ) p . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Further downstream , in the absence of EphB 6 , ZAP 70 activation , LAT phosphorylation , the association of PLCgamma 1 with SLP 76 , and p44 / 42 MAPK activation were diminished . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Following T cell receptor ligation , PLCgamma 1 interacts through its SH 2 and SH 3 domains with the adaptors LAT and SLP 76 , respectively , to form a multiprotein signaling complex that leads to activation of PLCgamma 1 by Syk tyrosine kinases . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Receptor and kinase activation is linked to distal signaling pathways ( PLC gamma 1 activation , Ca2+ influx , PKC activation and Ras / Erk activation ) via the adapter protein LAT ( Linker for Activation of T cells ) . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
Signaling events downstream of LAT ( PLCgamma 1 phosphorylation and p 85 [ PI 3 K ] association ) were impaired , whereas upstream events ( CD3zeta and ZAP 70 phosphorylation ) remained intact . ^^^
Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UFY1 and O43561	Pubmed	SVM Score :0.0
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