| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Monoclonal antibody 7H6 reacts with a novel tight junction associated protein distinct from ZO 1 , cingulin and ZO 2 . ^^^ These results strongly suggest that the 7H6 antibody recognizes a novel tight junction associated protein different from ZO 1 , cingulin and ZO 2 . . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| The effect on occludin was specific because other tight junction components , ZO 1 , ZO 2 , cingulin , and the adherens junction protein E cadherin , were unaltered by OCC 2 treatment . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Although immunocytochemical studies revealed only subtle changes in the distribution of the TJ proteins , zonula occludens ( ZO ) 1 , ZO 2 , and cingulin , examination of the Triton 10 100 solubilities of these proteins , an indicator of cytoskeletal association , revealed a striking shift of all three TJ proteins into the insoluble pool , consistent with increased cytoskeletal interaction during ATP depletion . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Nontransmembrane proteins on the cytosolic leaflet including ZO 1 , ZO 2 , cingulin , 7H6 , and several unidentified phosphoproteins are also believed to be part of the TJ . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Peripheral TJ proteins such as zonula occludens 1 ( ZO 1 ) , ZO 2 , and cingulin become extremely insoluble and associate into large macromolecular complexes ( T . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Cingulin contains globular and coiled coil domains and interacts with ZO 1 , ZO 2 , ZO 3 , and myosin . ^^^ Pull down assays from epithelial , insect cell , and reticulocyte lysates show that an NH ( 2 ) terminal fragment of cingulin ( 1 378 ) interacts in vitro with ZO 1 ( K ( d ) approximately 5 nM ) , ZO 2 , ZO 3 , myosin , and AF 6 , but not with symplekin , and a COOH terminal fragment ( 377 1 , 368 ) interacts with myosin and ZO 3 . ^^^ ZO 1 and ZO 2 immunoprecipitates contain cingulin , suggesting in vivo interactions . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Many ubiquitous molecular constituents of tight junctions have been identified and characterized including claudins , occludin , ZO 1 , ZO 2 , ZO 3 , cingulin , and 7H6 . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Cocultures were analyzed for paracellular permeability by measurement of transepithelial electrical resistance ( TER ) and for the tight junction proteins ZO 1 , ZO 2 , occludin , and cingulin by immunocytochemistry and immunoprecipitation . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Human and Xenopus cingulin heads are only 33 % identical , yet a human cingulin N terminal fragment still interacts with canine ZO 1 and ZO 2 in vitro . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Many ubiquitous molecular tight junction components have been identified and characterized including claudins , occludin , ZO 1 , ZO 2 , ZO 3 , cingulin and 7H6 . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| In good agreement , by immunofluorescence microscopy , most TJ proteins including claudins and occludin appeared to be normally concentrated at TJs of ZO 1 / cells with the exception that a ZO 1 deficiency significantly up or down regulated the recruitment of ZO 2 and cingulin , another TJ scaffold protein , respectively , to TJs . ^^^ This retardation as well as the disappearance of cingulin were rescued completely by exogenous ZO 1 but not by ZO 2 expression . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| In hetero and homozygous ( + / and / ) embryoid bodies , immunoblotting revealed a Triton soluble , truncated form of cingulin , increased levels of the tight junction proteins ZO 2 , occludin , claudin 6 and Lfc , and decreased levels of ZO 1 . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| We find that the HDAC inhibitor sodium butyrate significantly up regulates the protein levels of cingulin , ZO 1 , and ZO 2 in Rat 1 fibroblasts , cingulin in COS 7 cells , and cingulin and occludin in HeLa cells . ^^^ Levels of mRNA for cingulin , ZO 1 , and ZO 2 are also increased in sodium butyrate treated Rat 1 fibroblasts . ^^^ Up regulation of TJ proteins by sodium butyrate is linked to the ability of sodium butyrate to inhibit HDAC activity , because suberoylanilide hydroxamic acid , a HDAC inhibitor of a different structural class , also up regulates cingulin , ZO 1 , and ZO 2 expression in Rat 1 fibroblasts . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| The C terminus can also bind to the scaffolding proteins ZO 1 , ZO 2 , ZO 3 , cingulin , the membrane trafficking protein VAP 33 , and the cytoskeletal protein F actin , suggesting an important role for this domain . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| Depletion of ZO 2 , cingulin , or occludin , proteins that can interact with ZO 1 , had no discernible effects on tight junctions . ^^^ These data reveal an unexpected function for the SH 3 domain of ZO 1 in regulating tight junction assembly in epithelial cells and show that cingulin , occludin , or ZO 2 are not limiting for junction assembly in MDCK monolayers . . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| TJ proteins identified include transmembrane proteins ; occludin and claudin , and cytoplasmic plaque proteins ; ZO 1 , ZO 2 , ZO 3 , cingulin , and 7H6 . ^^^ |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9P2M7 and Q9UDY2 |
Pubmed |
SVM Score :0.0 |
| NA |
|