Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.73100042
The catalytic activity of Dnmt3A and 3B was stimulated approximately 15 fold , and Dnmt3L directly binds to DNA but not to S adenosyl L methionine ( AdoMet ) . 0.73100042^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The proposed mechanism for DNA ( cytosine 5 ) methyltransferases envisions a key role for a cysteine residue . ^^^ There is a single conserved cysteine among all DNA ( cytosine 5 ) methyltransferases making it the candidate nucleophile . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
F3mdCyd hemisubstituted M 13 DNA displayed the same properties as mdCyd substituted M 13 DNA with respect to cleavage by restriction enzymes , and acted as an efficient template for eukaryotic DNA methyltransferase ( S adenosyl L methionine DNA ( cytosine 5 ) methyltransferase : EC 2 . 1 . 1 . 37 ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The type 2 DNA ( cytosine 5 ) methyltransferase M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
HpaII ) , an example of a DNA ( cytosine 5 ) methyltransferase , was found to induce directly a high frequency of C > U transition mutations in double stranded DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
C base pairs may explain the de novo methylation of these helices by the human DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The properties of the methyl directed DNA ( cytosine 5 ) methyltransferase ( EC 2 . 1 . 1 . 37 ) suggest that it is the enzyme that maintains patterns of methylation in the human genome . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The arrangement of multiple motifs conserved in the prokaryotic genes is preserved in the human DNA MTase , including the relative position of a proline cysteine dipeptide thought to be an essential catalytic site in all ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We have examined differentiating male germ cells for alternative forms of DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) and have found a 6 . 2 kb DNA MTase mRNA that is present in appreciable quantities only in testis ; in post replicative pachytene spermatocytes it is the predominant form of DNA MTase mRNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Sequencing of the peptide indicated the DNA bound to a region of the protein that is conserved in all procaryotic DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mammalian DNA ( cytosine 5 ) methyltransferase contains a C terminal domain that is closely related to bacterial cytosine 5 restriction methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA ( cytosine 5 ) methyltransferase ( MTase ) transcript levels are over an order of magnitude higher throughout spermatogenesis than in non dividing liver cells . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recognition of foldback DNA by the human DNA ( cytosine 5 ) methyltransferase . ^^^ In order to specify the recognition requirements of the human DNA ( cytosine 5 ) methyltransferase , two isomeric 48mers were synthesized so as to link a long block of DNA with a shorter complementary block of DNA through a tether consisting of five thymidine residues . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The modified DNAs were less methylated in vitro than control DNAs by DNA ( cytosine 5 ) methyltransferase ( DNA methylase ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Effect of cobalamin derivatives on in vitro enzymatic DNA methylation : methylcobalamin can act as a methyl donor . 5 Methylcytosine synthesis in DNA involves the transfer of methyl groups from S adenosyl methionine to the 5 ' position of cytosine through the action of DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Wild type Escherichia coli cells containing elevated levels of DNA ( cytosine 5 ) methyltransferases have increased sensitivity to the toxic effects of 5 azacytidine . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The trypsin sensitive region of the EcoRII methyltransferase occurs prior to the first constant region shared with other procaryotic DNA ( cytosine 5 ) methyltransferases . ^^^ The N terminus of the EcoRII methylase , although a variable region present in many procaryotic DNA ( cytosine 5 ) methylases , plays no role in determining enzyme specificity , although it does contribute to the interaction with both AdoMet and DNA . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Direct identification of the active site nucleophile in a DNA ( cytosine 5 ) methyltransferase . ^^^ The overproduction , purification , and determination of the active site catalytic nucleophile of the DNA ( cytosine 5 ) methyltransferase ( DCMtase ) enzyme M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recognition of unusual DNA structures by human DNA ( cytosine 5 ) methyltransferase . ^^^ The symmetry of the responses of the human DNA ( cytosine 5 ) methyltransferase to alternative placements of 5 methylcytosine in model oligodeoxynucleotide duplexes containing unusual structures has been examined . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The steric course of methyl group transfer catalyzed by two DNA methylases , HhaI methylase , a DNA ( cytosine 5 ) methyltransferase , and EcoRI methylase , which methylates at N 6 of adenosine , has been studied with ( methyl R ) and ( methyl S ) [ methyl 2H1 , 3H ] adenosylmethionine as the methyl donor , using as substrates poly d ( GC ) ( HhaI ) and the dodecamer oligonucleotide duplex d ( CGCGAATTCGCG ) ( EcoRI ) , respectively . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
This region includes a highly conserved core sequence present in all DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Interaction of oligonucleotides containing 6 O methylguanine with human DNA ( cytosine 5 ) methyltransferase [ published erratumm appears in Biochemistry 1992 Aug 4 ; 31 ( 30 ) : 7008 ] . ^^^ C base pair at the 16th position ( i . e . , 5 ' CCCGTTTAAATATACXTATACCCGGGTACC 3 ' , where 10 = A or meG ) were used to study de novo methylation by the purified human DNA ( cytosine 5 ) methyltransferase isolated from CEM cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Binding of DNA ( cytosine 5 ) methyltransferases to azacytosine containing DNA is stimulated by the presence of S adenosyl methionine or its analogs sinefungin or S adenosyl L homocysteine . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Survival was partially lexA and recA dependent and was decreased by the presence of a DNA ( cytosine 5 ) methyltransferase . ^^^ The presence of DNA ( cytosine 5 ) methyltransferase had no effect on the mutation rate caused by 5 azacytidine treatment . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The irreversible binding of azacytosine containing DNA fragments to bacterial DNA ( cytosine 5 ) methyltransferases . ^^^ DNA containing 5 azacytosine is an irreversible inhibitor of DNA ( cytosine 5 ) methyltransferase . ^^^ No DNA protein complexes could be detected in E . coli B extracts , a strain that contains no DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The pvuIIM gene contains some sequences common to DNA methyltransferases in general , but includes none of the sequences specifically conserved among DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
To investigate the response of Saccharomyces cerevisiae to the presence of methylated bases , we introduced the Bacillus subtilis SPR phage DNA [ cytosine 5 ] methyltransferase gene on the shuttle vector , YEp 51 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Properties of DNA ( cytosine 5 ) methyltransferase in the brain ] . ^^^ Beef brain DNA ( cytosine 5 ) methyltransferase was partially purified by chromatography on Ultrogel AcA 34 and Dyematrex Blue A . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Size of the directing moiety at carbon 5 of cytosine and the activity of human DNA ( cytosine 5 ) methyltransferase . ^^^ M 13 DNAs in which carbon 5 of each deoxycytidine residue in one strand is replaced with a bulky group are very good substrates for human DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The influence of the dT . dG mispair on the activity of the human DNA ( cytosine 5 ) methyltransferase . ^^^ Synthetic oligodeoxynucleotides containing a dT . dG mispair at a centrally located d ( pCG ) dimer are methylated at a moderate rate by highly purified human DNA ( cytosine 5 ) methyltransferase ( E . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Stimulation of rat kidney , spleen and brain DNA ( cytosine 5 ) methyltransferases by divalent cobalt ions . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Structure of mouse DNA ( cytosine 5 ) methyltransferase . ^^^ DNA ( cytosine 5 ) methyltransferase was purified as a single polypeptide ( 190 kDa by SDS PAGE ) from mouse P 815 mastocytoma cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Variations of DNA ( cytosine 5 ) methyltransferase activities after administration of N hydroxy N aminofluorene to Sprague Dawley rats . ^^^ DNA methylation in eukaryotic cells is a post replicative process involving the transfer of methyl groups from S adenosyl L methionine to the 5 position of cytosine residues through the action of DNA ( cytosine 5 ) methyltransferase ( DNA methylase ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A cloning system for the DNA ( cytosine 5 ) methyltransferase MHhaI and high level expression of the enzyme are described . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Kinetic and catalytic properties of the DNA ( cytosine 5 ) methyltransferase HhaI are described . ^^^ Our studies reveal that the catalytic mechanism of DNA ( cytosine 5 ) methyltransferases involves attack of the C 6 of substrate cytosines by an enzyme nucleophile and formation of a transient covalent adduct . ^^^ This residue is found in a Pro Cys doublet which is conserved in all DNA ( cytosine 5 ) methyltransferases whose sequences have been determined to date and is found in related enzymes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
These were used to distinguish different kinetic mechanisms for maintenance and de novo methylation using a highly purified rat liver DNA ( cytosine 5 ) methyltransferase ( DMase+ ) preparation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Human DNA ( cytosine 5 ) methyltransferase selectively methylates duplex DNA containing mispairs . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Both the initial velocity and the overall methylation of DNA modified by acetylamino 4 , 6 dimethyldipyrido ( 1 , 2 a : 3 ' , 2 ' d ) imidazole ( A Glu P 3 ) by rat liver DNA ( cytosine 5 ) methyltransferase are decreased as compared to native DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Ethylation of poly ( dC dG ) . poly ( dC dG ) with ethyl methanesulfonate ( EtMes ) , a known carcinogen , at increasing molar ratios of EtMes / C 10 G base pairs progressively stimulated the methyl accepting ability of the DNA during in vitro methylation by partially purified rat DNA ( cytosine 5 ) methyltransferase ( EC 2 . 1 . 1 . 37 ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Friend murine erythroleukemia cells were found to contain three distinct species of DNA ( cytosine 5 ) methyltransferase ( DNA MeTase ) whose relative proportions were a characteristic function of the proliferative state of the cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The effect of pyrimidine photodimers on transmethylation reactions catalyzed by a highly purified rat liver DNA ( cytosine 5 ) methyltransferase ( EC 2 . 1 . 1 . 37 ) that exhibits maintenance and de novo methylation activities was studied in vitro , using the viral substrates M 13 mp9 replicative form ( RF ) DNA and the hemimethylated analog formed from primed synthesis of phage DNA in the presence of 2 ' deoxy 5 methylcytidine 5 ' triphosphate . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Changes in de novo DNA ( cytosine 5 ) methyltransferase activity in oncogenically susceptible rat target tissues induced by N methyl N nitrosourea . ^^^ The activity of de novo DNA ( cytosine 5 ) methyltransferase ( DNA methylase ) in various rat tissues after administration of a single dose of N methyl N nitrosourea ( MNU ) has been analyzed . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Purification of human DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The inhibition of DNA ( cytosine 5 ) methylases by 5 azacytidine . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Effect of 5 azacytidine on deoxyribonucleic acid methylation in Escherichia coli K 12 . 5 Azacytidine inhibits Escherichia coli DNA ( cytosine 5 ) methylase when added to growing cells . ^^^ When E . coli K 12 was treated with 5 azacytidine for 30 min , DNA ( cytosine 5 ) methylase levels decreased to less than 10 % of control levels and slowly recovered to control levels after seven generations of growth . 5 Methylcytosine synthesis in DNA remained at less than 10 % of control levels for three generations after treatment and returned to control levels after six generations of growth . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here , we show that treatment of L 1210 cells , grown intraperitoneally in mice , with 5 azacytidine led to a rapid and prolonged inactivation of DNA ( cytosine 5 ) methyltransferase activity and to the synthesis of undermethylated DNA . ^^^ DNA isolated from the treated tissue was found to inactivate the DNA methylase ( decreased Vmax ) in in vitro DNA ( cytosine 5 ) methyltransferase assays . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Two molecular weight forms of DNA ( cytosine 5 ) methyltransferase [ S adenosyl L methionine : DNA ( cytosine 5 ) methyltransferase , EC 2 . 1 . 1 . 37 ] , both active in assays in vitro , were isolated from the green alga Chlamydomonas reinhardi at various stages of the life cycle . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Two S adenosyl L methionine : DNA ( cytosine 5 ) methyltransferases , termed M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Several enzymes , for example DNA ( cytosine 5 ) methyltransferase , produce relatively strong interactions with DNA and hinder the quantitative recovery of this DNA from a reaction mixture . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Both the initial velocity and the overall methylation of DNA substituted by aminofluorene , by a rat liver DNA ( cytosine 5 ) methyltransferase , are increased as compared to native DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dye ligand chromatography on Cibacron blue F3GA agarose has been used to resolve two species of DNA ( cytosine 5 ) methyltransferase from nuclear extracts of uninduced Friend murine erythroleukemia cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Both the initial velocity and the overall methylation of Ac 4HAQO modified DNA by a calf brain DNA ( cytosine 5 ) methyltransferase are increased as compared to native DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The enzymatic methylation of chemically alkylated DNA and of poly ( dG dC ) 10 poly ( dG dC ) by beef brain DNA ( cytosine 5 ) methyltransferase have been tested . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA ( cytosine 5 ) methyltransferases can cause deamination of cytosine when the cofactor S adenosylmethionine ( AdoMet ) is limiting and thus function as sequence specific C > U mutator enzymes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Inhibition of transcription in vitro by binding of DNA ( cytosine 5 ) methylases to DNA templates containing cytosine analogs . ^^^ DNA ( cytosine 5 ) methylases form tight complexes at their methylation sites when the target cytosine residue is substituted by analogs such as 5 azacytosine or 5 fluorocytosine . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The mechanism of inhibition of DNA ( cytosine 5 ) methyltransferases by 5 azacytosine is likely to involve methyl transfer to the inhibitor . ^^^ The mechanism of inhibition of DNA ( cytosine 5 ) methyltransferases by the mechanism based inhibitor 5 azacytosine has remained unclear , mainly because of the unavailability of a substrate in which the inhibitor , but not normal cytosine , is present at the target site . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here we report the 2 . 8 A crystal structure of a bacterial DNA ( cytosine 5 ) methyltransferase ( DCMtase ) , M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mammalian DNA ( cytosine 5 ) methyltransferase expressed in Escherichia coli , purified and characterized . ^^^ DNA ( cytosine 5 ) methyltransferase catalyzes cytosine methylation in eukaryotes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Cytosine methylation is catalyzed by the enzyme DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The cytosine analog 5 azacytidine kills Escherichia coli cells that carry plasmids expressing EcoRII DNA ( cytosine 5 ) methyltransferase under control of its own promoter . ^^^ They showed that expression from the ecoRIIM promoter was inhibited when EcoRII DNA ( cytosine 5 ) methyltransferase was introduced into the cell in trans and inhibition was reversed by treating the cells with azacytidine . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Similar regulation is also noted in another DNA ( cytosine 5 ) methylase , M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
De novo methylation of normally unmethylated CpG islands and increased expression of DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) are common characteristics of immortalized cell lines and human tumors . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Methylation involves the addition of a methyl group to the 5 position of the cytosine base in DNA , a reaction catalysed by a DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We have shown a possible alternate mechanism for mutagenesis at CpG in which HpaII DNA ( cytosine 5 ) methyltransferase ( M . ^^^ Surprisingly , we found that DNA ( cytosine 5 ) methyltransferases have higher affinities for these DNA mismatches than for their normal G : C targets and are capable of transferring a methyl group to the 5 position of U , creating T at low efficiencies . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The ( cytosine 5 ) DNA methyltransferase M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Methylation of slipped duplexes , snapbacks and cruciforms by human DNA ( cytosine 5 ) methyltransferase . ^^^ When human DNA ( cytosine 5 ) methyltransferase was used to methylate a series of snapback oligodeoxy nucleotides of differing stem lengths , each containing a centrally located CG dinucleotide recognition site , the enzyme required a minimum of 22 base pairs in the stem for maximum activity . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Biological implications of the mechanism of action of human DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Unfortunately , DNA ( cytosine 5 ) methyltransferases ( EC 2 . 1 . 1 . 37 ) from various mammalian sources have been difficult to isolate and stabilize , precluding investigations of these critical enzymes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
These alignments suggest that many cytosine 5 MTases are likely to interact with DNA using a similar structural framework . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Expression and localization of the mRNA for DNA ( cytosine 5 ) methyltransferase in mouse seminiferous tubules . ^^^ DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) is the only enzyme known to be involved in the methylation of mammalian DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The DNA ( cytosine 5 ) methyltransferase ( m5C MTase ) M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methylation in mammalian cells is performed predominantly by the enzyme DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Crystallization and preliminary crystallographic analysis of a DNA ( cytosine 5 ) methyltransferase from Haemophilus aegyptius bound covalently to DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A new class of affinity labels has been developed for human DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The crystal structure has been determined at 2 . 8 A resolution for a chemically trapped covalent reaction intermediate between the HhaI DNA cytosine 5 methyltransferase , S adenosyl L homocysteine , and a duplex 13 mer DNA oligonucleotide containing methylated 5 fluorocytosine at its target . ^^^ The structure suggests how the active nucleophile reaches its target , directly supports the proposed mechanism for cytosine 5 DNA methylation , and illustrates a novel mode of sequence specific DNA recognition . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The regulation of DNA ( cytosine 5 ) methyltransferase ( DNA MeTase ) enzyme activity and gene expression was examined in the monoblastoid U 937 cell line induced to differentiate with either dibutyryl cyclic AMP ( dbcAMP ) or phorbol ester . dbcAMP treatment was found to cause the rapid ( < 4 h ) suppression of DNA MeTase specific activity , with no DNA MeTase activity detectable after 10 h . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We have found a motif present in All 1 that shows homology to the zinc binding domain of DNA ( cytosine 5 ) methyltransferases ( EC 2 . 1 . 1 . 63 ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The crystal structure of the DNA ( cytosine 5 ) methyltransferase , M . ^^^ The core of the structure is dominated by sequence motifs conserved among all DNA ( cytosine 5 ) methyltransferases , and these are responsible for cofactor binding and methyltransferase function . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
All DNA ( cytosine 5 ) methyltransferases contain a single conserved cysteine . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Embryonic stem ( ES ) cells homozygous for a disruption of the DNA ( cytosine 5 ) methyltransferase gene ( Dnmt ) proliferate normally with their DNA highly demethylated but die upon differentiation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
To determine whether this mechanism could contribute to the development of human colon cancer , we examined the level of DNA ( cytosine 5 ) methyltransferase ( MTase ) expression , the concentration of AdoMet , and the activity of uracil DNA glycosylase in human colon tissues , and searched for the presence of mutations in the MTase gene . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Characterisation of independent DNA and multiple Zn binding domains at the N terminus of human DNA ( cytosine 5 ) methyltransferase : modulating the property of a DNA binding domain by contiguous Zn binding motifs . ^^^ We report here a detailed mapping and characterisation of a DNA binding domain at the N terminus of human DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Methylation of cytosine is catalyzed by DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Deduced amino acid sequences of two clones showed high homology with human 10 linked Helicase 2 ( XH 2 ) and DNA ( cytosine 5 ) methyltransferase ( DMTase ) sequences , whereas the other two were not related to any known peptide sequence . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
De novo methylation of CpG island sequences in human fibroblasts overexpressing DNA ( cytosine 5 ) methyltransferase . ^^^ Recent studies showing a correlation between the levels of DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) enzyme activity and tumorigenicity have implicated this enzyme in the carcinogenic process . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mammalian DNA cytosine 5 methyltransferase interacts with p 23 protein . ^^^ These methylation patterns are established and maintained by DNA cytosine 5 methyltransferase ( MTase ) , a approximately 1500 amino acid enzyme containing a regulatory N terminal domain and a catalytic C terminal domain . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Methylation inhibitors can increase the rate of cytosine deamination by ( cytosine 5 ) DNA methyltransferase . ^^^ The target cytosines of ( cytosine 5 ) DNA methyltransferases in prokaryotic and eukaryotic DNA show increased rates of C > T transition mutations compared to non target cytosines . ^^^ We tested whether various inhibitors of ( cytosine 5 ) DNA methyltransferases analogous to AdoMet and AdoHcy would affect the rate of enzyme induced deamination of the target cytosine by M . ^^^ We have therefore identified the first mutagenic compounds specific for the target sites of ( cytosine 5 ) DNA methyltransferases . ^^^ Our findings show that chemotherapeutic agents with affinities to the cofactor binding pocket of ( cytosine 5 ) DNA methyltransferase should be tested for their potential mutagenic effects . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We have characterized the inhibition exerted by histone H 1 on the activity of human placenta DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Identification of the gene encoding the DNA ( cytosine 5 ) methyltransferase of lymphocystis disease virus . ^^^ The gene encoding the DNA ( cytosine 5 ) methyltransferase ( m5C MTase ) of lymphocystis disease virus ( flounder isolate , LCDV 1 ) has been identified by polymerase chain reaction ( PCR ) using oligonucleotide primers synthesized corresponding to different regions of the m5C MTase gene of frog virus 3 ( FV 3 ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In addition , the encoded protein , termed polybromo , contains four other domains : an unusual truncated HMG box , two repeats of a novel domain which we term the BAH domain and a sequence related to a region within the regulatory domain of the DNA cytosine 5 methyltransferase enzyme . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A full length cDNA , encoding a DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) , has been assembled from a series of overlapping cDNA clones isolated from P . lividus sea urchin embryo cDNA libraries . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
New 5 ' regions of the murine and human genes for DNA ( cytosine 5 ) methyltransferase . ^^^ DNA ( cytosine 5 ) methyltransferases ( EC 2 . 1 . 1 . 37 ) maintain patterns of methylated cytosine residues in the mammalian genome ; faithful maintenance of methylation patterns is required for normal development of mice , and aberrant methylation patterns are associated with certain human tumors and developmental abnormalities . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Chromosomal location of three wheat sequences with homology to pollen allergen encoding , DNA replication regulating , and DNA ( cytosine 5 ) methyltransferase genes in wheat and rye . ^^^ Three wheat sequences , shown to be homologous to pollen allergen encoding , DNA replication regulating , and DNA ( cytosine 5 ) methyltransferase genes were localized on chromosomes using nullisomic tetrasomic wheat ( ' Chinese Spring ' ) and wheat rye ( ' Chinese Spring ' / ' Imperial ' ) addition lines . ^^^ Whereas the loci for the pollen allergen encoding sequence ( Tri a 3 ) were shown to be located on homoeologous group 4 , the DNA replication regulating ( Rep ) and DNA ( cytosine 5 ) methyltransferase ( Mtase ) genes were located to homoeologous groups 1 and 7 , respectively , of Triticeae . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A remarkable feature of the catalytic mechanism of DNA ( cytosine 5 ) methyltransferases is the ability of these enzymes to induce deamination of the target cytosine in the absence of S adenosyl L methionine or its analogs . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
This discovery has led us to investigate the effects of the treatment of fission yeast with the nucleoside analogue 5 azacytidine ( 5 azaC ) . 5 AzaC is known to inhibit cytosine methylation as a result of the formation of stable covalent complexes between DNA ( cytosine 5 ) methyltransferases ( C 5 Mtases ) and 5 azaC containing DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Importantly , an analysis of the C termini of five known dsRNA adenosine deaminases , and two putative deaminases , reveals motifs that are strikingly similar to the conserved motifs of the DNA ( adenine N6alpha ) aminomethyltransferases and the DNA ( cytosine 5 ) methyltransferases . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A system for addressing in the construction of macromolecular assemblies can be based on the biospecificity of DNA ( cytosine 5 ) methyltransferases and the capacity of these enzymes to form abortive covalent complexes at targeted 5 fluorocytosine residues in DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We describe evidence for a sequence of events in which the Human DNA ( cytosine 5 ) methyl transferase first methylates spontaneous single stranded conformers ( SSCs ) and then stalls at the methylated site to produce a complex with the conformationally unusual DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Methylation spreading , which involves a propensity for the mammalian DNA ( cytosine 5 ) methyltransferase to de novo methylate cytosine guanine dinucleotides ( CpGs ) near pre existing 5 methylcytosine bases , has been implicated in the control of numerous biological processes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA ( cytosine 5 ) methyltransferases in mouse cells and tissues . ^^^ A universal mechanism based probe for DNA ( cytosine 5 ) methyltransferases was used to screen tissues and cell types known to be active in de novo methylation for new species of DNA methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recent investigations have shown that the maintenance of genomic imprinting of the murine insulin like growth factor 2 ( Igf 2 ) gene involves at least two factors : the DNA ( cytosine 5 ) methyltransferase activity , which is required to preserve the paternal specific expression of Igf 2 , and the H 19 gene ( lying 90 kb downstream of Igf 2 gene ) , which upon inactivation leads to relaxation of the Igf 2 imprint . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Human DNA ( cytosine 5 ) methyltransferase PCNA complex as a target for p21WAF1 . ^^^ DNA ( cytosine 5 ) methyltransferase ( MCMT ) methylates newly replicated mammalian DNA , but the factors regulating this activity are unknown . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dnmt 2 contains all the sequence motifs diagnostic of DNA ( cytosine 5 ) methyltransferases but appears to lack the large N terminal regulatory domain common to other eukaryotic methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Flipping of a nucleotide out of a B DNA helix into the active site of an enzyme has been observed for the HhaI and HaeIII cytosine 5 methyltransferases ( M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The mRNA for Dnmt 1 , the predominant maintenance and de novo DNA ( cytosine 5 ) methyl transferase in mammals , is present at high levels in postmitotic murine germ cells but undergoes alternative splicing of sex specific 5 ' exons , which controls the production and localization of enzyme during specific stages of gametogenesis . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Stalling of human DNA ( cytosine 5 ) methyltransferase at single strand conformers from a site of dynamic mutation . ^^^ Single strand conformers ( SSCs ) from the C rich strand of the triplet repeat at the FMR 1 locus are rapidly and selectively methylated by the human DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Using eukaryotic DNA ( cytosine 5 ) methyltransferase ( of both human and murine origin ) , we have studied the in vitro methylation pattern of three CpG islands . ^^^ SssI prokaryotic DNA ( cytosine 5 ) methyltransferase prior to in vitro methylation by the eukaryotic enzyme . ^^^ An A + T rich plasmid , pHb beta 1S , showed an initial stimulation , followed by a severe inhibition of the activity of DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A series of overlapping clones complementary to the Arabidopsis cytosine 5 DNA methyltransferase ( C 5 MTase ) has been isolated from pea cDNA libraries . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Most prokaryotic ( cytosine 5 ) DNA methyltransferases increase the frequency of deamination at the cytosine targeted for methylation in vitro in the absence of the cofactor S adenosylmethionine ( AdoMet ) or the reaction product S adenosylhomocysteine ( AdoHcy ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Because several lines of evidence indicate that inhibition of excess DNA ( cytosine 5 ) methyltransferase ( Mtase ) may be a sufficient factor for the suppression or reversion of carcinogenesis , we examined the effects of sodium selenite , BSC , p XSC and benzyl thiocyanate ( BTC ) , the sulfur analog of BSC , on Mtase activity in nuclear extracts of human colon carcinomas , and of p XSC on the Mtase activity of HCT 116 human colon carcinoma cells in culture . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The deduced amino acid sequence contains ten conserved motifs characteristic for [ cytosine 5 ] DNA methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Two recent reports have identified DNA ( cytosine 5 ) methyltransferase ( MCMT ) and the DNA repair endonuclease XPG as binding to PCNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Cloning and characterization of a family of novel mammalian DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In the present study , the isolation and characterization of two distinct cDNAs that code for carrot DNA ( cytosine 5 ) methyltransferase ( DNA METase ) are reported . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
It was discovered in 1994 when the first co crystal structure was reported for a cytosine 5 DNA methyltransferase binding to DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The major observation of this investigation is that a single intraperitoneal injection of butylated hydroxytoluene ( BHT , 60 mg / kg body mass ) results within a few hours in a strong increase in nuclear DNA ( cytosine 5 ) methyl transferase ( methyl transferase ) activity in the liver , kidneys , heart , spleen , brain and lungs of male rats . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Murine DNA ( cytosine 5 ) methyltransferase : steady state and substrate trapping analyses of the kinetic mechanism . ^^^ DNA ( cytosine 5 ) methyltransferase is essential for viable mammalian development and has a central function in the determination and maintenance of epigenetic methylation patterns . ^^^ HhaI , a bacterial DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
As a consequence of their mechanism of action , DNA ( cytosine 5 ) methyltransferases from both prokaryotes and eukaryotes necessarily recognize mispaired bases in unusual DNA structures as catalytic transition state analogs . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Expression of rat DNA ( cytosine 5 ) methyltransferase ( DNA MTase ) in rodent trophoblast giant cells : molecular cloning and characterization of rat DNA MTase . ^^^ A pattern of DNA methylation is maintained in mitotic cells by DNA ( cytosine 5 ) methytransferase ( DNA MTase ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Using sensitive methods of sequence analysis including hydrophobic cluster analysis , we report here a hitherto undescribed family of modules , the BAH ( bromo adjacent homology ) family , which includes proteins such as eukaryotic DNA ( cytosine 5 ) methyltransferases , the origin recognition complex 1 ( Orc 1 ) proteins , as well as several proteins involved in transcriptional regulation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
One of these encodes a putative DNA ( cytosine 5 ) methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
One potential mechanism is the up regulation of DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Downregulation of DNA ( cytosine 5 ) methyltransferase is a late event in NGF induced PC 12 cell differentiation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
However , this increase was not associated with modulation of DNA ( cytosine 5 ) methyltransferase , the enzyme that methylates eukaryotic DNA , which suggests that the changes in DNA methylation patterns are not linked to the process of de novo DNA methylation during cell death . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Effect of interaction between 5 azacytidine and DNA ( cytosine 5 ) methyltransferase on C to G and C to T mutations in Escherichia coli . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Down regulation of human DNA ( cytosine 5 ) methyltransferase induces cell cycle regulators p 16 ( ink4A ) and p 21 ( WAF / Cip1 ) by distinct mechanisms . ^^^ Methylation of 5 ' CpG islands of tumor suppressor genes and elevated levels of the DNA ( cytosine 5 ) methyltransferase enzyme ( DNA MeTase ) are also prevalent features of human neoplasia . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In normal somatic cells , the methylation pattern of DNA is stably maintained by DNA ( cytosine 5 ) methyltransferase ( DNA methyltransferase ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Two major forms of DNA ( cytosine 5 ) methyltransferase in human somatic tissues . ^^^ Thus far , only one major form of vertebrate DNA ( cytosine 5 ) methyltransferase ( CpG MTase , EC 2 . 1 . 1 . 37 ) has been identified , cloned , and extensively studied . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We have identified a total of 88 members of the DNA ( cytosine 5 ) methyltransferase ( 5mC MTase ) family whose sequences have been deposited in the databases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Characterization of a new variant DNA ( cytosine 5 ) methyltransferase unable to methylate double stranded DNA isolated from the marine annelid worm Chaetopterus variopedatus . ^^^ The enzyme S adenosylmethionine DNA ( cytosine 5 ) methyltransferase has been identified , first time for invertebrates , in embryos of the marine polychaete annelid worm Chaetopterus variopedatus . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mammalian ( cytosine 5 ) methyltransferases cause genomic DNA methylation and lethality in Drosophila . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recombinant human DNA ( cytosine 5 ) methyltransferase . 1 . ^^^ A method is described to express and purify human DNA ( cytosine 5 ) methyltransferase ( human DNMT 1 ) using a protein splicing ( intein ) fusion partner in a baculovirus expression vector . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recombinant human DNA ( cytosine 5 ) methyltransferase . 2 . ^^^ Initial velocity determinations were conducted with human DNA ( cytosine 5 ) methyltransferase ( DNMT 1 ) on unmethylated and hemimethylated DNA templates in order to assess the mechanism of the reaction . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Assignment of cytosine 5 DNA methyltransferases Dnmt3a and Dnmt3b to mouse chromosome bands 12A2 A 3 and 2H1 by in situ hybridization . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In mouse , this DNA ( cytosine 5 ) methyltransferase , or CpG MTase , is encoded by the Dnmt 1 gene . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Cytosine 5 DNA methylation occurs in the context of CpG dinucleotides in vertebrates . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Hybrid mouse prokaryotic DNA ( cytosine 5 ) methyltransferases retain the specificity of the parental C terminal domain . ^^^ The mouse ( cytosine 5 ) DNA methyltransferase ( Dnmt 1 ) consists of a regulatory N terminal and a catalytic C terminal domain , which are fused by a stretch of Gly Lys dipeptide repeats . ^^^ The C terminal region contains all of the conserved motifs found in other cytosine 5 DNA methyltransferases including the relative position of the catalytic Pro Cys dipeptide . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Primary structure and expression of the xiphophorus DNA ( cytosine 5 ) methyltransferase XDNMT 1 . ^^^ We have isolated a cDNA for the DNA ( cytosine 5 ) methyltransferase XDNMT 1 from this organism , which encodes the first full length protein from a fish species . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
As a target we used the mRNA of murine DNA ( cytosine 5 ) methyltransferase 1 ( MTase ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA bending induced by DNA ( cytosine 5 ) methyltransferases . ^^^ DNA bending induced by six DNA ( cytosine 5 ) methyltransferases was studied using circular permutation gel mobility shift assay . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Gilbert ' s conjecture : the search for DNA ( cytosine 5 ) demethylases and the emergence of new functions for eukaryotic DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNMT 2 is a human protein that displays strong sequence similarities to DNA ( cytosine 5 ) methyltransferases ( m ( 5 ) C MTases ) of both prokaryotes and eukaryotes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA ( cytosine 5 ) methyltransferase turnover and cellular localization in developing Paracentrotus lividus sea urchin embryo . ^^^ The turnover and localization of the enzyme DNA ( cytosine 5 ) methyltransferase ( Dnmt 1 ) were studied during Paracentrotus lividus sea urchin embryo development using antibody preparations against the NH ( 2 ) and COOH terminal regions of the molecule . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
EcoRII DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Enzymatic properties of de novo type mouse DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Cloning and sequence analysis of a zebrafish cDNA encoding DNA ( cytosine 5 ) methyltransferase 1 . ^^^ We report the determination of the full length cDNA sequence corresponding to the zebrafish DNA ( cytosine 5 ) methyltransferase gene , Dnmt 1 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Five novel alternatively spliced transcripts of DNA ( cytosine 5 ) methyltransferase 2 in human peripheral blood leukocytes . ^^^ Alternative splicing of RNA molecules transcribed from DNA ( cytosine 5 ) methyltransferases has been proposed as a mechanism by which methylation is able to effect diverse biological processes in higher eukaryotes . ^^^ This study has investigated transcriptional versatility of DNA ( cytosine 5 ) methyltransferase 2 , which may methylate cytosine residues within 5 ' CCTGG 3 ' pentanucleotides in regions of the human genome devoid of 5 ' CG 3 ' methylation . ^^^ Five novel splice variants of DNA ( cytosine 5 ) methyltransferase 2 were identified in the peripheral blood leukocytes of healthy subjects following cloning and sequencing of RT PCR products amplified using gene specific oligodeoxyribonucleotide primers . ^^^ The DNA ( cytosine 5 ) methyltransferase 2 splice variants are generated in all the major cell types of peripheral blood , as well as in neoplastic lymphoid cells indicating that they are unlikely to generate proteins involved in control of the cell cycle or cellular differentiation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Since methylcytosine is relatively unstable , a deficiency of CpG dinucleotides and accumulation of mutations that manifest as TpG ( and its complement CpA ) is a diagnostic feature of higher eukaryotic DNA sequences subjected to methylation by DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The retinoblastoma gene product interacts with maintenance human DNA ( cytosine 5 ) methyltransferase and modulates its activity . ^^^ The mammalian DNA ( cytosine 5 ) methyltransferase ( Dnmt 1 ) is involved in the maintenance of methylation patterns in the genome during DNA replication and development . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Selective depletion of human DNA methyltransferase DNMT 1 proteins by sulfonate derived methylating agents . 5 Methylcytosine residues in the DNA ( DNA methylation ) are formed from the transfer of the methyl group from S adenosylmethionine to the C 5 position of cytosine by the DNA ( cytosine 5 ) methyltransferases ( DNMTs ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Measurement of genome wide DNA cytosine 5 methylation by reversed phase high pressure liquid chromatography . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mouse DNA ( cytosine 5 ) methyltransferases Dnmt3a and Dnmt3b are expected to be de novo type DNA methyltransferases . ^^^ On the other hand , neither bacterial DNA ( cytosine 5 ) methyltransferase nor Dnmt3b3 , one of the three isoforms of Dnmt3b that has no DNA methylation activity , induced apoptosis . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Methylation of 5 ' CpG islands of tumor suppressor genes and elevated levels of the DNA ( cytosine 5 ) methyltransferase enzymes ( DNMT 1 , 3A and 3B ) are also prevalent features of human neoplasia . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Co operation and communication between the human maintenance and de novo DNA ( cytosine 5 ) methyltransferases . ^^^ Three different families of DNA ( cytosine 5 ) methyltransferases , DNMT 1 , DUMT 2 , DNMT3a and DNMT3b , participate in establishing and maintaining genomic methylation patterns during mammalian development . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Unexpectedly , five of the new phosphoCTD associating proteins ( PCAPs ) represent either enzymes that act on DNA and chromatin ( topoisomerase 1 , DNA ( cytosine 5 ) methyltransferase 1 , poly ( ADP ribose ) polymerase 1 ) or proteins known to bind DNA ( heterogeneous nuclear ribonucleoprotein ( hnRNP ) U / SAF A , hnRNP D ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Investigating the target recognition of DNA cytosine 5 methyltransferase HhaI by library selection using in vitro compartmentalisation . ^^^ In vitro compartmentalisation ( IVC ) , a technique for selecting genes encoding enzymes based on compartmentalising gene translation and enzymatic reactions in emulsions , was used to investigate the interaction of the DNA cytosine 5 methyltransferase M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Variations in DNA ( cytosine 5 ) methyltransferase 1 expression during oogenesis and early development of the zebrafish . ^^^ We report the determination of zebrafish DNA ( cytosine 5 ) methyltransferase ( dnmt 1 ) temporal and spatial patterns of expression in gonadal tissues and during early development . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Features of methylation dysregulation in many different types of neoplasms include general genomic hypomethylation , focal hypermethylation , and altered expression of genes which encode a series of DNA ( cytosine 5 ) methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Structural organization of the sea urchin DNA ( cytosine 5 ) methyltransferase gene and characterization of five alternative spliced transcripts . ^^^ Sea urchin DNA ( cytosine 5 ) methyltransferase ( Dnmt 1 ) that is responsible for maintenance of DNA methylation patterns clearly shares similarity with various Dnmt1s identified in vertebrates . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dysregulation and instability of DNA methylation and alterations in the levels of the predominant DNA methylating enzyme , DNA ( cytosine 5 ) methyltransferase 1 ( Dnmt 1 ) , have also been linked to tumorigenesis . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We find that cloned preimplantation mouse embryos aberrantly express the somatic form of the Dnmt 1 DNA ( cytosine 5 ) methyltransferase , the expression of which is normally prevented by a posttranscriptional mechanism . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
While CpG methylation can be readily analyzed at the DNA sequence level in wild type and mutant cells , the actual DNA ( cytosine 5 ) methyltransferases ( DNMTs ) responsible for in vivo methylation on genomic DNA are less tractable . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Allosteric activator domain of maintenance human DNA ( cytosine 5 ) methyltransferase and its role in methylation spreading . ^^^ The human maintenance DNA ( cytosine 5 ) methyltransferase ( hDNMT 1 ) consists of a large N terminal regulatory domain fused to a catalytic C terminal domain by randomly repeated Gly Lys dipeptides . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Effects of cadmium on DNA ( Cytosine 5 ) methyltransferase activity and DNA methylation status during cadmium induced cellular transformation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
CviPI , a cytosine 5 DNA methyltransferase recognizing GC sites , is fused to a DNA binding factor enabling simultaneous detection of targeted methylation , factor footprints , and chromatin structural changes by bisulfite genomic sequencing . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The eukaryotic DNMT 2 genes encode a new class of cytosine 5 DNA methyltransferases . ^^^ DNMT 2 is a subgroup of the eukaryotic cytosine 5 DNA methyltransferase gene family . ^^^ These data provide solid evidence that the fly and mouse DNMT 2 gene products are genuine cytosine 5 DNA methyltransferases . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Methods for the design and analysis of oligodeoxynucleotide based DNA ( cytosine 5 ) methyltransferase inhibitors . ^^^ Several second generation inhibitors of DNA ( cytosine 5 ) methyltransferases based on studies of modified synthetic oligodeoxynucleoides have been described . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Analysis of DNA ( cytosine 5 ) methyltransferase mRNA sequence and expression in bovine preimplantation embryos , fetal and adult tissues . ^^^ To date , the vast majority of work concerning the developmental expression of the DNA cytosine 5 methyltansferases ( Dnmts ) has been conducted in mice . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The preference of murine DNA ( cytosine 5 ) methyltransferase ( Dnmt 1 ) for single stranded DNA substrates is increased up to 50 fold by the presence of a proximal 5 methyl cytosine ( 5 ( me ) C ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Two genomic clones ( OsMET 1 1 , AF 462029 and OsMET 1 2 , TPA BK 001405 ) , each encoding a cytosine 5 DNA methyltransferase ( MTase ) , were isolated from rice ( Oryza sativa L . ) BAC libraries . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A cDNA coding for a DNA ( cytosine 5 ) methyltransferase ( METase ) was isolated from peach ( Prunus persica [ L . ] Batsch ) and the corresponding gene designated as PpMETI . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA ( cytosine 5 ) methyltransferase ( DNMT ) catalyzes the transfer of a methyl group from S adenosyl L methionine ( SAM ) to C 5 of cytosine within CpG dinucleotide sequences in the genomic DNA of higher eukaryotes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mammalian DNA ( cytosine 5 ) methyltransferases and their expression . ^^^ Two classes of functional DNA ( cytosine 5 ) methyltransferases have been discovered in mammals to date . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Site selective in vivo targeting of cytosine 5 DNA methylation by zinc finger proteins . ^^^ Cytosine 5 DNA methylation is a critical signal defining heritable epigenetic states of transcription . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA ( cytosine 5 ) methyltransferase 1 ( DNMT 1 ) plays an important role in the maintenance of DNA methylation patterns via complicated networks including signaling pathways and transcriptional factors , relating to cell differentiation or carcinogenesis . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA ( cytosine 5 ) methyltransferase ( Dnmt ) , a methylating enzyme of cytosine residues on CpG dinucleotides , plays an important role in 10 chromosome inactivation , genomic imprinting , and gene expression . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The protozoan parasite Entamoeba histolytica express a cytosine 5 DNA methyltransferase ( Ehmeth ) that belongs to the DNMT 2 protein family . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Human maintenance DNA ( cytosine 5 ) methyltransferase and p 53 modulate expression of p 53 repressed promoters . ^^^ DNA ( cytosine 5 ) methyltransferase ( DNMT ) 1 participates in transcriptional repression of genes by methylation dependent and independent mechanisms . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Of particular interest , we found that both DNMT3B ( DNA ( cytosine 5 ) methyltransferase 3 beta ) and DNMT3L ( DNA ( cytosine 5 ) methyltransferase 3 like ) were overexpressed in the N SEMs , indicating the epigenetic differences between N SEMs and classical SEM . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
There are multiple families of DNA ( cytosine 5 ) methyltransferases in eukaryotes , and each family appears to be controlled by different regulatory inputs . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
These genes include several that have recently become associated with carcinogenesis , such as Krppel like factor ( KLF ) 4 , a gut enriched transcription factor associated with induction of differentiation and reduction in cellular proliferation ; DNA ( cytosine 5 ) methyltransferase 1 , associated with methylation ; and alpha methylacyl CoA racemase ( AMACR ) , a marker associated with the development of colon and prostate cancer . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The protozoan parasite Entamoeba histolytica expresses a cytosine 5 DNA methyltransferase ( Ehmeth ) that belongs to the DNMT 2 protein family . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The protozoan parasite Entamoeba histolytica expresses a cytosine 5 DNA methyltransferase ( Ehmeth ) that belongs to the Dnmt 2 proteins family . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
An orphan DNA ( cytosine 5 ) methyltransferase in Vibrio cholerae . 5 Methyl cytosine ( m5C ) was detected in genomic DNA of the enteric pathogen Vibrio cholerae by HPLC analysis and immunoblotting with m5C specific antibody . ^^^ Although cleavage with the restriction endonuclease EcoRII revealed the absence of a Dcm homologue in 5 . cholerae , analysis of the genome sequence indicated the presence of a gene , designated in this study as vchM , which encodes a DNA ( cytosine 5 ) methyltransferase ( m5C MTase ) designated M . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dnmt 2 enzymes have been widely conserved during evolution and contain all of the signature motifs of DNA ( cytosine 5 ) methyltransferases ; however , the DNA methyltransferase activity of these proteins is comparatively weak and their biochemical and functional properties remain enigmatic . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Significant regulations could be analyzed for 5 genes ( expression > 2 or < 0 . 5 fold ) : IL15RA ( Interleukin 15 receptor , alpha chain ) , EPS15R ( Epidermal growth factor receptor pathway substrate 15 like 1 ) , DNMT3A ( Hypothetical protein MGC 16121 ) , DNMT3A ( DNA ( cytosine 5 ) methyltransferase 3 alpha ) , and one gene with no match to known genes , DKFZP586J1624 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA damage induced down regulation of human Cdc25C and Cdc 2 is mediated by cooperation between p 53 and maintenance DNA ( cytosine 5 ) methyltransferase 1 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We have investigated the distribution of DNA methylation in chromosomes and nuclei of normal individuals and ICF ( Immunodeficiency , Centromeric instability and Facial abnormalities ) syndrome patients , using 5 methylcytosine monoclonal antibody . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
An embryonic like methylation pattern of classical satellite DNA is observed in ICF syndrome . ^^^ When ICF DNA was tested with methyl sensitive enzymes , several classical satellite families , but not alphoid sequences , showed a very low level of methylcytosine in leukocyte DNA , with an abnormal pattern compared to the normal germinal and extraembryonic methylation profile . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We found that conditioned medium from ICF exposed cultures stimulated [ 3H ] TdR incorporation into DNA , and [ 3H ] proline incorporation into collagenase digestible protein but not into non collagen protein in fresh calvarial cultures . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA , FISH and complementation studies in ICF syndrome : DNA hypomethylation of repetitive and single copy loci and evidence for a trans acting factor . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The overlap of the spectrum of chromosomal rearrangements in azaCR or azaCdR treated FLEB 14 cells and in mitogen stimulated lymphocytes from patients with a rare genetic disease ( ICF ) associated with localized DNA hypomethylation supports the hypothesis that the DNA demethylating activity of azaCR is essential for the induction of these pericentromeric rearrangements . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methylation in normal individuals and in ICF patients : heterogeneous methylation of constitutive heterochromatin in adult and fetal tissues . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Evidence that DNA ( cytosine 5 ) methyltransferase regulates synaptic plasticity in the hippocampus . ^^^ DNA ( cytosine 5 ) methylation represents one of the most widely used mechanisms of enduring cellular memory . ^^^ In this study , we examined the role DNA ( cytosine 5 ) methyltransferase ( DNMT ) activity might play in regulating the induction of synaptic plasticity . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The methylation profile exhibited in ICF patients reproduces the normal profile of placental or sperm DNA . ^^^ The DNA methylation defect in ICF patients , first detected in satellite DNAs ( constitutive heterochromatin ) and CpG islands of genes on the inactive 10 chromosome ( facultative heterochromatin ) , thus includes Alu sequences that are widely distributed throughout the human genome . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A major component of the pericentromeric DNA in chromosome 1 , satellite 2 , was shown to be hypomethylated in an ICF B cell line , although DNA from this cell line did not display detectable overall hypomethylation . ^^^ It is hypothesized that demethylation in certain DNA regions , including in pericentromeric satellite DNA , helps lead to pericentromeric chromosomal rearrangements in lymphocytes from ICF patients and in normal lymphoblastoid cells incubated in vitro with DNA demethylating agents . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Drug induced DNA demethylation in normal human cells and inherited localized hypomethylation in mitogen stimulated lymphocytes from patients with a rare recessive disease ( ICF : immunodeficiency , centromeric region instability , facial anomalies ) are associated with karyotypic instability . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
ICF patients show marked hypomethylation of their DNA ; undermethylation of classical satellites 2 and 3 is thought to be associated with the centromere instability . ^^^ We used DNA from three consanguineous families with a total of four ICF patients and performed a total genome screen , to localize the ICF syndrome gene by homozygosity mapping . ^^^ Isolation of the gene associated with the ICF syndrome not only will give insight into the etiology of the ICF syndrome but will also broaden our understanding of DNA methylation processes . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA hypomethylation has also been associated with abnormal chromosomal structures , as observed in cells from patients with ICF ( Immunodeficiency , Centromeric instability and Facial abnormalities ) syndrome and in cells treated with the demethylating agent 5 azadeoxycytidine . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Rearrangements in these regions and hypomethylation of satellite 2 DNA are a characteristic feature of patients with a rare recessive genetic disease , ICF ( immunodeficiency , centromeric region instability , and facial anomalies ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The recent cloning of a new family of DNA methyltransferases ( Dnmt3a and Dnmt3b ) in mouse which methylate hemimethylated and unmethylated templates with equal efficiencies make them candidates for the long sought de novo methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA undermethylation is a characteristic feature of ICF syndrome and has been implicated in the formation of the juxtacentromeric chromosomal abnormalities of this rare syndrome . ^^^ Our results suggest that the genetic alteration of DNA methylation in ICF syndrome has little consequence on 10 chromosome gene expression and chromatin organization . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA hypomethylation and unusual chromosome instability in cell lines from ICF syndrome patients . ^^^ The ICF syndrome ( immunodeficiency , centromeric region instability , facial anomalies ) is a unique DNA methylation deficiency disease diagnosed by an extraordinary collection of chromosomal anomalies specifically in the vicinity of the centromeres of chromosomes 1 and 16 ( Chr 1 and Chr 16 ) in mitogen stimulated lymphocytes . ^^^ The ICF specific hypomethylation occurs in only a small percentage of the genome , e . g . , ICF brain DNA had 7 % less 5 methylcytosine than normal brain DNA . ^^^ The ICF lymphoblastoid cell lines , therefore , retain not only the ICF specific pattern of chromosome rearrangements , but also of targeted DNA hypomethylation . ^^^ This hypomethylation of heterochromatic DNA sequences is seen in many cancers and may predispose to chromosome rearrangements in cancer as well as in ICF . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development . ^^^ Here we demonstrate that two recently identified DNA methyltransferases , Dnmt3a and Dnmt3b , are essential for de novo methylation and for mouse development . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The DNMT3B DNA methyltransferase gene is mutated in the ICF immunodeficiency syndrome . ^^^ By searching for homologies to known DNA methyltransferases , we identified a genomic sequence in the ICF region that contains the homologue of the mouse Dnmt3b methyltransferase gene . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Classical satellite DNA is normally heavily methylated at cytosine residues , but in ICF syndrome it is almost completely unmethylated in all tissues . ^^^ Here we show that five unrelated ICF patients have mutations in both alleles of the gene that encodes DNA methyltransferase 3B ( refs 5 , 6 ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Whole genome methylation scan in ICF syndrome : hypomethylation of non satellite DNA repeats D4Z4 and NBL 2 . ^^^ The ICF ( immunodeficiency , centromeric instability and facial abnormalities ) syndrome is a rare recessive disease characterized by immunodeficiency , extraordinary instability of certain heterochromatin regions and mutations in the gene encoding DNA methyltransferase 3B . ^^^ However , ICF DNA digests prominently displayed multicopy fragments absent in controls . ^^^ The high degree of methylation of D4Z4 that we observed in normal cells may be related to the postulated role of this DNA repeat in position effect variegation in facio scapulohumeral muscular dystrophy and might also pertain to abnormal gene expression in ICF . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The generation and proper maintenance of DNA methylation patterns are essential for embryonic development , as demonstrated by the lethal phenotypes of mice with either a targeted disruption of Dnmt 1 , the gene responsible for the maintenance of DNA methylation , or targeted disruption of Dnmt3a or Dnmt3b , the genes involved in generation of newly formed methylation patterns . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A human genetic disorder ( ICF syndrome ) has recently been shown to be caused by mutations in the DNA methyltransferase 3B ( DNMT3B ) gene . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Genome demethylation and chromosome instability could not be related to variations in mRNA amounts of the DNA methyltransferases DNMT 1 , DNMT3A , and DNMT3B and DNA demethylase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We , as well as others , have found that these effects derive from mutations in the DNMT3B DNA methyltransferase gene . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Immunodeficiency , centromeric region instability , and facial anomalies ( ICF ) , a rare recessive chromosome instability syndrome , involves the loss of DNA methyltransferase 3B activity and the consequent hypomethylation of a small portion of the genome . ^^^ ICF associated undermethylation of some regulatory gene ( s ) might lead to an exaggerated response to radiation induced breaks in DNA yielding increased rates of cell death and irreversible cell cycle arrest . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Positional candidate cloning recently discovered the de novo DNA methyltransferase 3B ( DNMT3B ) as the responsible gene by identifying seven different mutations in nine ICF patients . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Over expression of DNMT 1 mRNA was significantly associated with CIMP , whereas the level of DNMT3b mRNA was not associated with CIMP or DNA hypomethylation of peri centromeric satellite regions . ^^^ These data suggest that both over expression of the maintenance DNA methyltransferase DNMT 1 and over expression of a newly identified de novo DNA methyltransferase , DNMT3b , are involved in human carcinogenesis , probably at different stages and through different mechanisms . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recently identified new DNA methyltransferase ( DNMT ) genes , DNMT3A and DNMT3B , code for de novo methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methylation has recently moved to centre stage in the aetiology of human neurodevelopmental syndromes such as the fragile 10 , ICF and Rett syndromes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Inactivation of the DNA methyltransferase genes ( Dnmt 1 , 3a , and 3b ) was found to be lethal in mice and several human diseases ( ICF and Rett syndrome ) turned out to be linked to DNA methylation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We demonstrate that the recently identified DNA methyltransferases , Dnmt3a and Dnmt3b , like DNMT 1 , repress transcription in a methylation independent manner . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Satellite DNA hypomethylation has been postulated as the mechanism underlying the induction of chromosome 1 peri centromeric instability in many human cancers and in individuals with the rare recessive disorder ICF ( immunodeficiency , centromeric heterochromatin instability , facial anomalies ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The subcellular distribution of the recently discovered de novo DNA methyltransferases , Dnmt3a and Dnmt3b , was investigated by immunofluorescence and by epitope tagging . ^^^ We now show that both Dnmt3a and Dnmt3b are distributed throughout the nucleoplasm but are not associated with nuclear DNA replication sites during S phase . ^^^ These results suggest that de novo methylation by Dnmt3a and Dnmt3b occurs independently of the replication process and might involve an alternative mechanism for accessing the target DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mutation in the DNMT3B DNA methyltransferase gene is a common cause of ICF ( immunodeficiency , centromeric heterochromatin , facial anomalies ) immunodeficiency syndrome and leads to hypomethylation of satellites 2 and 3 in pericentric heterochromatin . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Like repeated DNA sequences in the juxtacentromeric heterochromatin of chromosomes 1 , 9 , and 16 , D4Z4 was hypomethylated at numerous CpGs in sperm and in cell lines from patients with an unrelated DNA methyltransferase deficiency syndrome ( ICF ; immunodeficiency , centromeric region instability , facial anomalies ) in contrast to its hypermethylation in non ICF postnatal somatic tissues . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dnmt3b , de novo DNA methyltransferase , interacts with SUMO 1 and Ubc 9 through its N terminal region and is subject to modification by SUMO 1 . ^^^ Dnmt3b , a DNA methyltransferase , is essential for mammalian development potentially through its transcription repression activity . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methyltransferase 3B mutations linked to the ICF syndrome cause dysregulation of lymphogenesis genes . ^^^ ICF ( immunodeficiency , centromeric region instability and facial anomalies ) is a recessive disease caused by mutations in the DNA methyltransferase 3B gene ( DNMT3B ) . ^^^ ICF ( immunodeficiency , centromeric region instability and facial anomalies ) is a recessive disease caused by mutations in the DNA methyltransferase 3B gene ( DNMT3B ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
To date , three enzymes , Dnmt 1 , Dnmt3a , and Dnmt3b , are known to have DNA methyltransferase activity in mouse and human . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We examined levels of DNA methyltransferase ( DNMT 1 , DNMT3a , DNMT3b ) and DNA demethylase ( MBD 2 ) mRNA expression by semi quantitative RT PCR . ^^^ There was no clear relation between DNA methylation status of hMLH 1 , p 16 ( INK4a ) , and CDH 1 and the mRNA expression levels of DNMT 1 , DNMT3a , DNMT3b or MBD 2 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Although enzymes have been identified that can methylate DNA de novo ( Dnmt3a and Dnmt3b ) ( 14 ) , it is unknown how specific patterns of methylation are established in the genome . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
OBJECTIVE : The aim of this study was to investigate whether expression of the enzymes that catalyze cytosine CpG island methylation , DNA methyltransferases , DNMT 1 , DNMT3a , and DNMT3b is altered in human ovarian cancer . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA binding folds . ^^^ The PWWP domain is a weakly conserved sequence motif found in > 60 eukaryotic proteins , including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The enzymes responsible for CpG methylation are DNA methyltransferase ( DNMT ) 1 , DNMT3a , and DNMT3b , and the enzyme responsible for demethylation is DNA demethylase ( MBD 2 ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Molecular enzymology of the catalytic domains of the Dnmt3a and Dnmt3b DNA methyltransferases . ^^^ The C terminal domains of the mammalian DNA methyltransferases Dnmt 1 , Dnmt3a , and Dnmt3b harbor all the conserved motifs characteristic for cytosine C 5 methyltransferases . ^^^ However , the catalytic domain of Dnmt3a methylates DNA in a distributive reaction , whereas Dnmt3b is processive , which accelerates methylation of macromolecular DNA in vitro . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Surprisingly , however , genetic disruption of both DNMT 1 and DNMT3b nearly eliminated methyltransferase activity , and reduced genomic DNA methylation by greater than 95 % . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In this study , we demonstrate that Dnmt3L , a protein sharing homology with DNA methyltransferases , Dnmt3a and Dnmt3b , but lacking enzymatic activity , is essential for the establishment of maternal methylation imprints and appropriate expression of maternally imprinted genes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
An essential role for DNA methyltransferase DNMT3B in cancer cell survival . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methyltransferases , Dnmt3a and Dnmt3b , are required for de novo methylation in embryonic stem ( ES ) cells and postimplantation embryos . ^^^ In this study , we have analyzed the sequence specificity of Dnmt3a and Dnmt3b during de novo methylation of murine Moloney leukemia virus provirus DNA in virus infected ES cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A DNA methyltransferase , DNMT3b , is required for methylation on pericentromeric satellite regions during mouse development . ^^^ To clarify the molecular mechanism underlying DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis , we examined mutations of the DNMT3b gene and mRNA expression levels of splice variants of DNMT3b in noncancerous liver tissues showing chronic hepatitis and cirrhosis , which are considered to be precancerous conditions , and in hepatocellular carcinomas ( HCCs ) . ^^^ Overexpression of DNMT3b4 , a splice variant of DNMT3b lacking conserved methyltransferase motifs 9 and 10 , significantly correlated with DNA hypomethylation on pericentromeric satellite regions in precancerous conditions and HCCs ( P = 0 . 0001 ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The immunodeficiency , centromeric region instability , facial anomalies ( ICF ) syndrome , a rare recessive DNA methyltransferase deficiency disease , results in a small decrease in the extent of global genomic methylation . ^^^ In ICF , DNA hypomethylation is targeted to the satellite DNA in juxtacentromeric ( centromere adjacent ) heterochromatin of chromosomes 1 and 16 ( 1qh and 16qh ) , which are prone to rearrangements in ICF lymphoid cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methylation regulates important biological processes and is involved in tumorigenesis and several human diseases , such as Rett and immunodeficiency , centromeric instability and facial anomalies ( ICF ) . ^^^ In search of enzymes responsible for de novo methylation , we have cloned a novel family of mammalian DNA methyltransferase genes , Dnmt3a and Dnmt3b . ^^^ Additionally , biochemical analysis revealed that , unlike Dnmt 1 , neither Dnmt3a nor Dnmt3b had a strong preference to hemimethylated DNA substrates . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNMT3L shows high similarity to the de novo DNA methyltransferases , DNMT3A and DNMT3B , however , the amino acid residues needed for DNA cytosine methyltransferase activity have been lost from the DNMT3L protein sequence . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Defective de novo methylation of viral and cellular DNA sequences in ICF syndrome cells . ^^^ ICF syndrome ( immunodeficiency , centromere instability and facial anomalies ) is a recessive human genetic disorder resulting from mutations in the DNA methyltransferase 3B ( DNMT3B ) gene . ^^^ Global levels of DNA methylation in ICF cells are only slightly reduced ; however , certain repetitive sequences and genes on the inactive 10 chromosome of female ICF patients are significantly hypomethylated . ^^^ Lastly , no consistent changes in the protein levels of the DNA methyltransferases were noted when normal and ICF cell lines were compared . . ^^^ ICF syndrome ( immunodeficiency , centromere instability and facial anomalies ) is a recessive human genetic disorder resulting from mutations in the DNA methyltransferase 3B ( DNMT3B ) gene . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recently , we identified a C > T transition at a novel promoter region of cytosine DNA methyltransferase 3B ( DNMT3B ) and found that this polymorphic transition significantly increases the promoter activity . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
It is caused by mutations in a de novo DNA methyltransferase gene , DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
No relationship was observed between global genomic 5 methylcytosine levels and relative amounts of RNA for DNA methyltransferases DNMT 1 , DNMT3A , and DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Two DNA methyltransferases , Dnmt3a and Dnmt3b , contribute to the creation of DNA methylation patterns in embryos . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The human DNA methyltransferases DNMT3A and DNMT3B have two types of promoters with different CpG contents . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
RESULTS : The cDNA isolated in our hands was one of the alternative splicing isoforms of mouse de novo DNA cytosine 5 ' specific methyltransferase gene ( Dnmt3b ) reported in July , 1998 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
ICF patients have constitutive hypomethylation at satellite 2 DNA ( Sat 2 ) in 1qh and 16qh , generally as the result of mutations in the DNA methyltransferase gene DNMT3B . ^^^ ICF patients have constitutive hypomethylation at satellite 2 DNA ( Sat 2 ) in 1qh and 16qh , generally as the result of mutations in the DNA methyltransferase gene DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
To analyse the protein structure and consequences of ICF causing mutations , we modelled the structure of the DNMT3B methyltransferase domain based on Haemophilus haemolyticus protein in complex with the cofactor AdoMet and the target DNA sequence . ^^^ Based on the model , the DNMT3B recognizes the GC sequence and flips the cytosine from the double stranded DNA to the catalytic pocket . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Following purification , the majority of de novo DNA methyltransfearse activity was associated with Dnmt3b / Dnmt1 fractions . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The present study was designed to investigate the potential relationship between CDKN2A ( p 16 ) gene hypermethylation , which has reported to be frequently observed in oral squamous cell carcinomas ( OSCCs ) , and expression of human DNA methyltransferases ( DNMTs : DNMT 1 , DNMT3A and DNMT3B ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
BACKGROUND : In mammals , epigenetic information is established and maintained via the postreplicative methylation of cytosine residues by the DNA methyltransferases Dnmt 1 , Dnmt3a and Dnmt3b . ^^^ Contrary to Dnmt3a or Dnmt3b , the isolated C terminal region of Dnmt 1 is catalytically inactive , despite the presence of the sequence motifs typical of active DNA methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The DNA methyltransferases ( MTases ) Dnmt3a and Dnmt3b are thought to be the sole de novo MTases in the mammalian genome . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
This hypomethylation is found in both colorectal cancers and normal mucosa from the same patients , and in cell lines with somatic cell knockout of DNA methyltransferases DNMT 1 and DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In ICF syndrome , a human disease affecting DNA methylation , SYBL 1 escapes from silencing and this correlates with altered patterns of histone methylation and acetylation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In contrast , targeted disruption of DNMT 1 alleles in HCT 116 human colon cancer cells produced clones that retained CpG island methylation and associated tumor suppressor gene silencing , whereas HCT 116 clones with inactivation of both DNMT 1 and DNMT3B showed much lower levels of DNA methylation , suggesting that the two enzymes are highly cooperative . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
To explore the role of DNA methyltransferases ( Dnmt ) in acquired drug resistance of neuroblastoma , the present investigation was carried out to study the expression of Dnmtl , Dnmt3a , and Dnmt3b in drug resistant murine neuroblastoma cells , in an in vitro model system . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here we report a functional search for hypermethylated CpG islands using the colorectal cancer cell line HCT 116 , in which two major DNA methyltransferases , DNMT 1 and DNMT3b , have been genetically disrupted ( DKO cells ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Cells from ICF patients who are deficient in one of the DNA methyltransferases , DNMT3B , provide an opportunity to explore and refine this hypothesis . ^^^ The DNMT3B methyltransferase , therefore , is required for methylation of L 1 CpG islands on the inactive 10 , whereas methylation of the corresponding L 1 loci on the active 10 , as well as most autosomal L1s , is accomplished by another DNA methyltransferase . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The Dnmt3b gene encodes a de novo DNA methyltransferase that is essential for normal mouse development . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Moreover , CpG methylation and thus silencing of CHFR depended on the activities of two DNA methyltransferases , DNMT 1 and DNMT3b , as their genetic inactivation restored CHFR expression . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Comparison to the recently reported structure of a homologous domain from the mammalian DNA methyltransferase Dnmt3b reveals substantial differences both in the C terminal helical region and in the PWWP motif . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
RESULTS : Here , we demonstrate a physical and functional link between the Suv39h HP 1 histone methylation system and DNA methyltransferase 3b ( Dnmt3b ) in mammals . ^^^ While the Suv39h HMTases are required to direct H 3 K9 trimethylation and Dnmt3b dependent DNA methylation at pericentric repeats , DNA methylation at centromeric repeats occurs independent of Suv39h function . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Distinct enzymatic properties of recombinant mouse DNA methyltransferases Dnmt3a and Dnmt3b . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here we show that a de novo DNA methyltransferase , DNMT3b , substantially contributes to the oncogenic phenotype in a lung cancer model . ^^^ While expression of TSCL 1 correlated with methylation of CpG dinucleotides in its promoter region , the expression of FHIT did not , suggesting that DNMT3b may silence genes by several mechanisms including direct DNA methylation or recruitment of proteins that modify chromatin . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We have previously shown that the DNA methyltransferases Dnmt3a and Dnmt3b carry out de novo methylation of the mouse genome during early postimplantation development and of maternally imprinted genes in the oocyte . ^^^ In the present study , we demonstrate that Dnmt3a and Dnmt3b are also essential for the stable inheritance , or `` maintenance , ' ' of DNA methylation patterns . ^^^ We also show that hypermethylation of genomic DNA by Dnmt3a and Dnmt3b is necessary for ES cells to form teratomas in nude mice . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mutations in the DNMT3B DNA methyltransferase gene cause the ICF immunodeficiency syndrome . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
PURPOSE : Three genes , namely DNA methyltransferase ( DNMT ) 1 , DNMT3A , and DNMT3B , coding for DNMTs that affect promoter methylation status are thought to play an important role in the development of cancers . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Double RNA interference of DNMT3b and DNMT 1 enhances DNA demethylation and gene reactivation . ^^^ In this study , we used specific siRNAs as a tool to probe the relationship between two DNA methyltransferase genes , DNMT3b and DNMT 1 , in the maintenance of DNA methylation patterns in the genome . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recent studies have shown that the methyltransferases DNMT 1 and DNMT3b cooperatively maintain DNA methylation and gene silencing in human cancer cells . ^^^ Disruption of the human DNMT3b only slightly reduces the overall global DNA methylation ; however , demethylation was markedly potentiated when both DNMT 1 and DNMT3b were simultaneously deleted . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The ICF syndrome , a DNA methyltransferase 3B deficiency and immunodeficiency disease . ^^^ These rearrangement prone regions show DNA hypomethylation in all examined ICF cell populations . ^^^ This review summarizes our knowledge about the immunological symptoms of ICF ; the nature of DNMT3B mutations in ICF patients ; the phenotypes of DNA hypomethylation mutants in humans , mice , and Arabidopsis ; the epigenetics of ICF ; and ICF specific RNA expression and cell surface antigen expression in lymphoblastoid cell lines . ^^^ Comparisons of ICF and control lymphoblastoid cell lines and ICF patients ' symptoms suggest an involvement of DNA methylation in the late stages of lymphocyte maturation . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mammalian DNA methylation patterns are established and maintained by co operative interactions among the Dnmt proteins Dnmt 1 , Dnmt3a and Dnmt3b . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mutations in the DNA methyltransferase 3B ( DNMT3B ) gene are responsible for most ICF cases reported . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The functional significance of PWWP mediated chromatin targeting is suggested by the fact that a missense mutation in this domain of human DNMT3B causes immunodeficiency , centromeric heterochromatin instability , facial anomalies ( ICF ) syndrome , which is characterized by loss of methylation in satellite DNA , pericentromeric instability , and immunodeficiency . ^^^ Two de novo DNA methyltransferases , Dnmt3a and Dnmt3b , are responsible for the process . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We compared the temporal expression patterns of the DNA methyltransferases , DNMT 1 , DNMT3a , DNMT3b , and DNMT3l in the male and female germ lines . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methylation is mediated by DNA methyltransferases ( DNMTs ) , of which three active forms have been identified : DNMT 1 , DNM3A and DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In mammals , DNA methylation is mediated by at least four DNA methyltransferase ( Dnmt ) enzymes , including Dnmt 1 , Dnmt 2 , Dnmt3a , and Dnmt3b . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
To elucidate how DNA methyltransferases ( Dnmts ) participate in methylation of the genomic components , we investigated the genome wide DNA methylation pattern of the T DMRs with Dnmt 1 , Dnmt3a , and / or Dnmt3b deficient ES cells by restriction landmark genomic scanning ( RLGS ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
It remains unclear , however , exactly how chromatin and epigenetic chromatin modifications affect the biological properties of the DNA methyltransferases ( DNMT 1 , DNMT3A , and DNMT3B ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNMT3L stimulates the DNA methylation activity of Dnmt3a and Dnmt3b through a direct interaction . ^^^ Two DNA methyltransferases , Dnmt3a and Dnmt3b , are responsible for the creation of DNA methylation patterns . ^^^ In the present study , the effect of DNMT3L , a human homologue of Dnmt3L , on the DNA methylation activity of mouse Dnmt3a and Dnmt3b was examined in vitro . ^^^ DNMT3L enhanced the DNA methylation activity of Dnmt3a and Dnmt3b about 1 . 5 3 fold in a dose dependent manner but did not enhance the DNA methylation activity of Dnmt 1 . ^^^ DNMT3L could not bind to DNA but could bind to Dnmt3a and Dnmt3b , indicating that the stimulatory effect of DNMT3L on the DNA methylation activity may not be due to the guiding of Dnmt3a and Dnmt3b to the targeting DNA sequence but may comprise a direct effect on their catalytic activity . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
De novo DNA methyltransferase DNMT3B is critical for embryonic development and is mutated in ICF syndrome . ^^^ Here we demonstrate that DNMT3B associates with four chromatin associated enzymatic activities common to transcriptionally repressed , heterochromatic regions of the genome : DNA methyltransferase , histone deacetylase , ATPase , and histone methylase activities . ^^^ Our results therefore link DNMT3B to three other components of the epigenetic machinery and provide important insights into how DNA methylation patterns may be established within the chromatin environment . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The de novo DNA methyltransferase DNMT3B is of particular interest because it is frequently overexpressed in tumor cells and is mutated in immunodeficiency , centromere instability and facial anomalies ( ICF ) syndrome . ^^^ Isolation and characterization of a novel DNA methyltransferase complex linking DNMT3B with components of the mitotic chromosome condensation machinery . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Although de novo DNA methyltransferases of the Dnmt 3 family are implicated in maternal imprinting , the lethality of Dnmt3a and Dnmt3b knockout mice has precluded further studies . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The Immunodeficiency , Centromeric instability , and Facial ( ICF ) syndrome is a rare autosomal recessive disorder that results from mutations in the DNMT3B gene , encoding a DNA methyltransferase that acts on GC rich satellite DNAs . ^^^ In order to investigate the effect of DNA hypomethylation on heterochromatin organization , we analyzed the in vivo distribution of HP 1 proteins , essential components of heterochromatin , in three ICF patients . ^^^ Finally , satellite DNA length polymorphism could affect the efficiency of heterochromatin condensation and thus contribute to the variability of the ICF phenotype . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We present the data on tissue specificity in radiation induced expression of DNA methyltransferases , and prove that changes in the expression of de novo methyltransferases DNMT3a and DNMT3b are the most important in radiation induced DNA methylation alterations . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
ICF syndrome in a girl with DNA hypomethylation but without detectable DNMT3B mutation . ^^^ A 3 year old girl with phenotypic and cytogenetic manifestations of the ICF syndrome and DNA hypomethylation but without DNMT3B gene mutation is described . ^^^ ICF syndrome in a girl with DNA hypomethylation but without detectable DNMT3B mutation . ^^^ Hypomethylation of classical satellite 2 DNA was observed with BstBI digestion , but in a lesser degree than those in the individuals with proven DNMT3B mutations . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The DNMT3A ( DNA methyltransferase 3A ) and DNMT3B genes encode putative de novo methyltransferases and show complex transcriptional regulation in the presence of three and two different promoters respectively . ^^^ The importance of these Sp 1 binding sites was demonstrated by using a GC rich DNA binding protein inhibitor , mithramycin A , i . e . on the basis of decrease in the promoter activities and mRNA expression levels of DNMT3A and DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In ICF cells , however , genes subject to 10 inactivation are hypomethylated on the inactive 10 due to mutations in the DNA methyltransferase ( DNMT3B ) genes . ^^^ Therefore , if DNA methylation is upstream of histone modification , the histones on the inactive 10 in ICF cells should not be modified to a silent form . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The PWWP domain of Dnmt3a and Dnmt3b is required for directing DNA methylation to the major satellite repeats at pericentric heterochromatin . ^^^ Dnmt3a and Dnmt3b are responsible for the establishment of DNA methylation patterns during development . ^^^ Furthermore , we demonstrate that the Dnmt3a PWWP domain has little DNA binding ability , in contrast to the Dnmt3b PWWP domain , which binds DNA nonspecifically . ^^^ Collectively , our results suggest that the PWWP domains of Dnmt3a and Dnmt3b are essential for targeting these enzymes to pericentric heterochromatin , probably via a mechanism other than protein DNA interactions . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The methylation and resultant silencing of CIITA PIV depended on the activities of two DNA methyltransferases , DNMT 1 and DNMT3B , and their genetic inactivation restored CIITA PIV expression . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
OBJECTIVE : To understand the role of epigenetic regulation in the pathogenesis of endometrial cancer , we have characterized DNA methyltransferase 3B ( DNMT3B ) gene expression in normal , Grade 1 and Grade 3 endometrioid cancers , and examined DNMT3B promoter activities in endometrial cancer cell lines . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system . ^^^ To explore the role of DNA methylation in the brain , we examined the expression pattern of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the mouse central nervous system ( CNS ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here we review our current understanding of the molecular enzymology of the mammalian DNA methyltransferases Dnmt 1 , Dnmt3a , Dnmt3b and Dnmt 2 and the roles of the enzymes in the above mentioned biological processes . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methyltransferase 3B ( DNMT3B ) plays an important role in the generation of aberrant methylation in carcinogenesis . ^^^ Polymorphisms and haplotypes of the DNMT3B gene may influence DNMT3B activity on DNA methylation , thereby modulating the susceptibility to lung cancer . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Two de novo type DNA methyltransferases , Dnmt3a and Dnmt3b , are responsible for the creation of DNA methylation patterns during development . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A deeper knowledge about their apotosis inducing mechanisms and their interaction with DNA methyltransferases ( DNMTs ) DNMT 1 , DNMT3a , and DNMT3b might allow the design of more effective drugs with lower cytotoxicity . 5 aza cytidine ( 5 aza CR ) , a potent inhibitor of DNMT 1 , is known to induce demethylation and reactivation of silenced genes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In the present study , we examined which de novo type DNA methyltransferase , Dnmt3a , Dnmt3a2 or Dnmt3b is expressed in gonocytes at these stages . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNMT3B mutations and DNA methylation defect define two types of ICF syndrome . ^^^ DNMT3B mutations and DNA methylation defect define two types of ICF syndrome . ^^^ Mutations in the catalytic domain of DNMT3B , a gene encoding a de novo DNA methyltransferase , have been recognized in a subset of patients . ^^^ The variable incidence of DNMT3B mutations and the differential methylation defect of alpha satellites allow the identification of two types of patients , both showing an undermethylation of classical satellite DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Real time PCR and Western blot techniques were employed to measure the mRNA and protein levels of the four DNA methyltransferases , DNMT 1 , DNMT 2 , DNMT3A , and DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5 aza 2 ' deoxycytidine . ^^^ These results suggest that the cytotoxic effect of 5 aza dC may be mediated primarily through Dnmt3a and Dnmt3b de novo DNA methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methyltransferase ( DNMT ) 3A and DNMT3B are both active de novo DNA methyltransferases required for development , whereas DNMT3L , which has no demonstrable methyltransferase activity , is required for methylation of imprinted genes in the oocyte . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Inactivation of Dnmt3b in mouse embryonic fibroblasts results in DNA hypomethylation , chromosomal instability , and spontaneous immortalization . ^^^ In this study , we have investigated how targeted disruption of the DNA methyltransferases Dnmt3a and Dnmt3b affects the growth of mouse embryonic fibroblasts ( MEFs ) . ^^^ Our studies led to the following observations . 1 ) Constitutive or conditional deletion of Dnmt3b , but not Dnmt3a , resulted in partial loss of DNA methylation throughout the genome , suggesting that Dnmt3b , in addition to the major maintenance methyltransferase Dnmt 1 , is required for maintaining DNA methylation in MEF cells . 2 ) Dnmt3b deficient MEF cells showed aneuploidy and polyploidy , chromosomal breaks , and fusions . 3 ) Inactivation of Dnmt3b resulted in either premature senescence or spontaneous immortalization of MEF cells . 4 ) The G ( 1 ) to S phase checkpoint was intact in primary and spontaneously immortalized Dnmt3b deficient MEFs because the p 53 protein was inducible by DNA damage . ^^^ These results suggest that DNA hypomethylation may induce genomic instability , which in turn leads to spontaneous immortalization or premature senescence of Dnmt3b deficient MEFs via a p 53 independent mechanism . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
BACKGROUND : Though Dnmt 1 is considered the primary maintenance methyltransferase and Dnmt3a and Dnmt3b are considered de novo methyltransferases in mammals , these three enzymes may work together in maintaining as well as establishing DNA methylation patterns . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here we describe a mechanism by which HDAC inhibitors affect DNA methylation through their regulation on DNMT3B , a methyltransferase responsible for de novo DNA methylation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The expression of de novo DNA methylase DNMT3b , of the methyl CpG binding protein MBD2b and of 5 MCDG glycosylase shows two waves of induction during CaCO 2 cell differentiation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Previously , it was shown that NBL 2 , a complex tandem DNA repeat in the acrocentric chromosomes , is hypomethylated at NotI sites in > 70 % of neuroblastomas and hepatocellular carcinomas and in cells from ICF syndrome ( DNMT3B deficiency ) patients . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Profound flanking sequence preference of Dnmt3a and Dnmt3b mammalian DNA methyltransferases shape the human epigenome . ^^^ We have investigated the influence of flanking sequence on the catalytic activity of the Dnmt3a and Dnmt3b de novo DNA methyltransferases using a set of synthetic oligonucleotide substrates that covers all possible + / 1 flanks in quantitative terms . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here we show that in lymphoblastoid cell lines from four ICF patients , there was increased colocalization of the hypomethylated 1qh and 16qh sequences in interphase , abnormal looping of pericentromeric DNA sequences at metaphase , formation of bridges at anaphase , chromosome 1 and 16 fragmentation at the telophase interphase transition , and , in apoptotic cells , micronuclei with overrepresentation of chromosome 1 and 16 material . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
To further elucidate the mechanism by which DNMT3L stimulates DNA methylation , we have mapped in detail the domains that mediate interaction of human DNMT3L with human DNMT3A and DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here we report that among three functional DNA methyltransferases ( DNMT 1 , DNMT3A , and DNMT3B ) , the maintenance methyltransferase , DNMT 1 , was rapidly degraded by the proteasomal pathway upon treatment of cells with these drugs . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Using a laser microirradiation system to introduce DNA lesions at defined subnuclear sites , we tested whether the major DNA methyltransferase ( Dnmt 1 ) or one of the two de novo methyltransferases ( Dnmt3a , Dnmt3b ) are recruited to sites of DNA repair in vivo . ^^^ Time lapse microscopy of microirradiated mammalian cells expressing GFP tagged Dnmt 1 , Dnmt3a , or Dnmt3b1 together with red fluorescent protein tagged proliferating cell nuclear antigen ( PCNA ) revealed that Dnmt 1 and PCNA accumulate at DNA damage sites as early as 1 min after irradiation in S and non S phase cells , whereas recruitment of Dnmt3a and Dnmt3b was not observed . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
AIM : To investigate the association between single nucleotide polymorphism ( SNP ) in promoter of the DNA methyltransferase 3B ( DNMT3B ) gene and risk for development and lymphatic metastasis of gastric cardiac adenocarcinoma ( GCA ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Several potential acceptors of cycle generated methyl groups , the DNA methyltransferases ( DNMT 1 , DNMT3A , DNMT3B and DNMT3L ) , glycine methyltransferase and the polyamine biosynthetic enzymes , SAM decarboxylase and ornithine decarboxylase , were also expressed . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
A C / T polymorphism in the DNA methyltransferase 3b ( DNMT3b ) promoter region results in increased activity and has recently been identified as a risk factor for lung cancer . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Moreover , expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b was essential for repression and DNA methylation of the Ant 4 gene during ESC differentiation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
MNB cells overexpressing DNA methyltransferase activity ( Dnmt3a or Dnmt3b ) were established by stable co transfection of wild type MNB cells with plasmids containing Dnmt3a or Dnmt3b cDNA . ^^^ Cytotoxic response ( IC 50 ) , total DNA methyltransferase activity and expression of Dnmt3a or Dnmt3b methyltransferase were determined in Dnmt3a or Dnmt3b transfected MNB cells , respectively . ^^^ RESULTS : These data demonstrated that total DNA methyltransferase activity was increased to 3 fold above controls ( P < 0 . 001 ) in cisplatin resistant MNB cells , 3 fold in Dnmt3a and 4 fold in Dnmt3b transfected MNB cells . ^^^ Incubation of cisplatin resistant , Dnmt3a or Dnmt3b overexpressing MNB cells with 5 ' azacytidine ( 5 ' azaC ) , a methylation inhibitor ( 2 . 5 microM ) significantly decreased DNA methyltransferase activity , expression of Dnmt3a and Dnmt3b proteins and mRNA levels of cisplatin resistant , Dnmt3a and Dnmt3b transfected MNB cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Finally , we show that hypomethylation is associated with reduced levels of the de novo DNA methyltransferases Dnmt3a and Dnmt3b and that ectopic expression of these factors restores global methylation levels . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Expression of another de novo DNA methyltransferase DNMT3b was decreased only in males . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
To understand the chromatin remodeling activities in cloned embryos and to improve NT technology , we examined the expression profiles of five genes involved in DNA and histone modifications , DNMT 1 , DNMT3A , DNMT3B , HAT 1 and HDAC 1 , in single swamp buffalo metaphase 2 oocytes , NT and in vitro fertilized ( IVF ) embryos from the two cell to the blastocyst stage , by quantitative real time RT PCR . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dnmt3a and Dnmt3b are de novo DNA methyltransferases that also act as transcriptional repressors independent of methyltransferase activity . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here , we show an interaction between PU . 1 and DNA methyltransferases , DNA methyltransferase ( Dnmt ) 3a and Dnmt3b ( Dnmt3s ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
OBJECTIVE : DNA methyltransferase ( DNMT ) 1 , DNMT3b , or both , facilitate malignant transformation through chromatin remodeling mechanisms . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dnmt3a and Dnmt3b are two major de novo DNA methyltransferases essential for embryonic development in mammals . ^^^ However , the exact target CpG sites where Dnmt3a and Dnmt3b catalyze DNA methylation remains largely unknown . ^^^ To identify a CpG site that is specifically methylated by Dnmt3a or Dnmt3b , we screened methylated genomic loci by methylation sensitive restriction fingerprinting using genomic DNA from wild type , Dnmt3a null , Dnmt3b null , and Dnmt3a Dnmt3b double null ES cells . ^^^ Exogenous expression of Dnmt3a but not Dnmt3b in the double null ES cells restored DNA methylation of this CpG site . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In contrast to SYBL 1 , the inactive 10 and Y alleles of SPRY 3 are not reactivated in cells treated with a DNA methylation inhibitor and in cells from ICF ( immunodeficiency , centromeric instability , facial anomalies ) syndrome patients , which have mutations in the DNA methyltransferase gene DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
ICF ( Immunodeficiency , Centromeric instability and Facial anomalies ) syndrome is a rare autosomal recessive disease caused by mutations in the DNA methyltransferase gene DNMT3B . ^^^ ICF ( Immunodeficiency , Centromeric instability and Facial anomalies ) syndrome is a rare autosomal recessive disease caused by mutations in the DNA methyltransferase gene DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Using an isogenic panel of cell lines proficient or deficient in the DNA methyltransferases ( DNMTs ) DNMT 1 and / or DNMT3B , we show that hypermethylation of the WIF 1 promoter is attributable to the cooperative activity of both DNMT 1 and DNMT3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Correspondently , the protein levels of DNA methyltransferase 1 ( DNMT 1 ) and DNMT3a increase from young to middle aged fibroblasts but decrease in the senescent fibroblasts , while DNMT3b decreases stably from young to senescent fibroblasts . p 21 ( Waf1 / Cip1 ) promoter methylation directly represses its expression and blocks the radiation induced DNA damage signaling pathway by p 53 in middle aged fibroblasts . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Cancer linked satellite DNA hypomethylation was independent of RNA levels for all DNMT3B isoforms , despite the ICF syndrome linked DNMT3B deficiency causing juxtacentromeric satellite DNA hypomethylation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Previously we showed that DNA methyltransferase 3b ( Dnmt3b ) is required for nerve growth factor ( NGF ) induced differentiation of PC 12 cells to neuronal phenotype . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mutations in DNA methyltransferase DNMT3B in ICF syndrome affect its regulation by DNMT3L . ^^^ Deficiency in DNA methyltransferase DNMT3B causes a recessive human disorder characterized by immunodeficiency , centromeric instability and facial anomalies ( ICF ) in association with defects in genomic methylation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Inhibition of DNA methyltransferase ( Dnmt ) enzymes with 5 aza 2 ' deoxycytidine or genetic ablation of both Dnmt 1 and Dnmt3b prevented promoter methylation and restored CXCL 12 expression . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
DNA methyltransferase 3b ( DNMT3b ) , an enzyme that participates in the establishment of de novo methylation patterns , is highly expressed in many tumor cells and tissues , and it is closely associated with hypermethylation of the promoter of tumor suppressor genes . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In mammals 3 families of DNA methyltransferases ( MTases ) comprising ( so far ) 4 members have been found : Dnmt 1 , Dnmt 2 , Dnmt3A and Dnmt3B . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Protein expressions of maintenance ( DNMT 1 ) DNA methyltransferase and de novo DNA methyltransferases DNMT3a and DNMT3b were decreased at all time points . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
SETDB 1 interacts with the de novo DNA methyltransferases DNMT3A and DNMT3B but not with the maintenance methyltransferase DNMT 1 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Subsequently , the expression levels of the DNA methyltransferases Dnmt 1 , Dnmt3a and Dnmt3b and the transcription factors Sp 1 and Sp 3 , which have been reported to regulate the expression of Dnmts , were examined at days 5 , 14 and 30 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
ICF always involves limited hypomethylation of DNA and often arises from mutations in one of the DNA methyltransferase genes ( DNMT3B ) . ^^^ ICF always involves limited hypomethylation of DNA and often arises from mutations in one of the DNA methyltransferase genes ( DNMT3B ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We compared the temporal expression patterns of the postulated de novo DNA methyltransferases DNMT3a and DNMT3b in murine male germ cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In addition , disrupting the expression of either one of two DNA methyltransferases ( DNMT 1 or DNMT3B ) by specific siRNAs abolished the siRNA mediated methylation of DNA . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Maintenance of self renewal ability of mouse embryonic stem cells in the absence of DNA methyltransferases Dnmt 1 , Dnmt3a and Dnmt3b . ^^^ DNA methyltransferases Dnmt 1 , Dnmt3a and Dnmt3b cooperatively regulate cytosine methylation in CpG dinucleotides in mammalian genomes , providing an epigenetic basis for gene silencing and maintenance of genome integrity . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The methylation of genomic DNA in malignant cells is catalyzed by DNA methyltransferases DNMT 1 and DNMT3B , revealing significantly elevated expression in different types of cancers . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Rather , in vitro analysis indicates that Dnmt3L stimulates DNA methylation by both Dnmt3a and Dnmt3b through direct binding to these proteins . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
We have isolated and sequenced the mouse zinc finger gene , Dnmt3l ( DNA cytosine 5 methyltransferase 3 like ) , on mouse chromosome 10 , showing similarity to members of the DNMT3 / Dnmt3 family . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Bisulfite genomic sequencing of DNA from oocytes and embryos showed that removal of Dnmt3L prevented methylation of sequences that are normally maternally methylated . ^^^ The key catalytic motifs characteristic of DNA cytosine methyltransferases have been lost from Dnmt3L , and the protein is more likely to act as a regulator of imprint establishment than as a DNA methyltransferase . . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The Dnmt3L protein belongs to the Dnmt 3 family of DNA methyltransferases by virtue of its sequence homology in the plant homeodomain ( PHD ) like motif . ^^^ Dnmt3L is essential for the establishment of maternal genomic imprints and , given its lack of key methyltransferase motifs , is more likely to act as a regulator of methylation rather than as an enzyme that methylates DNA . ^^^ Here , we show that Dnmt3L , like Dnmt3a and Dnmt3b , interacts both in vitro and in vivo with the histone deacetylase HDAC 1 . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dnmt3L , an isoform of Dnmt3a and Dnmt3b , but lacking enzymatic activity , interacts with Dnmt2a and Dnmt3b and is required for spermatogenesis . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The DNA methyltransferase like protein DNMT3L stimulates de novo methylation by Dnmt3a . ^^^ Dnmt3L , however , lacks the conserved catalytic domain common to DNA methyltransferases . ^^^ The implications of these findings for the function of DNMT3L and Dnmt3a in DNA methylation and genomic imprinting are discussed . . ^^^ In an attempt to define its function , we coexpressed DNMT3L with each of the two known de novo methyltransferases , Dnmt3a and DNMT3B , in human cells and monitored de novo methylation by using replicating minichromosomes carrying various ICs as targets . ^^^ Coexpression of DNMT3L with DNMT3B led to little or no change in target methylation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In the mouse , mutations of the oocyte specific isoform of the DNA methyltransferase Dnmt 1 ( Dnmt1o ) and of the methyltransferase like Dnmt3L gene result in specific failures of imprint establishment or maintenance , at multiple loci . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In addition , we show that methylation acquisition was probably related to oocyte diameter and coincided with the accumulation of Dnmt3a , Dnmt3b and Dnmt3L transcripts . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dnmt3L ( DNA cytosine 5 methyltransferase 3 like ) , a member of de novo methyltransferase Dnmt 3 protein family , is a regulator of maternal imprinting . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Here we show that DNA methyltransferase 3 like ( Dnmt3L ( ref . 1 ) ) is expressed in testes during a brief perinatal period in the non dividing precursors of spermatogonial stem cells at a stage where retrotransposons undergo de novo methylation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Dnmt3L , a DNA methyltransferase regulator , is expressed during gametogenesis , and its deletion results in sterility . ^^^ We found that during spermatogenesis , Dnmt3L contributes to the acquisition of DNA methylation at paternally imprinted regions , unique nonpericentric heterochromatic sequences , and interspersed repeats , including autonomous transposable elements . ^^^ We observed retrotransposition of an LTR ERV 1 element in the DNA from Dnmt3L / germ cells , presumably as a result of hypomethylation . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The aim of this study was to analyze the expression patterns of DNMT 1 , DNMT 2 , DNMT3A , DNMT3B , and DNMT3L genes in rhesus macaque ( Macaca mulatta ) oocytes and preimplantation stage embryos from fertilization to the hatched blastocyst stage , and to compare these results with the expression profiles in the mouse and other mammalian species . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The DNA methyltransferase like protein Dnmt3L is necessary for the establishment of genomic imprints in oogenesis and for normal spermatogenesis ( Bourc ' his et al . , 2001 ; Hata et al . , 2002 ) . ^^^ Also , a paternally imprinted gene , H 19 , loses DNA methylation in Dnmt3L / spermatogonia ( Bourc ' his and Bestor , 2004 ; Kaneda et al . , 2004 ) . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
The mammalian DNMTs are DNMT 1 , DNMT3A and DNMT3B , which together with accessory proteins , like DNMT3L , are responsible for methylation pattern acquisition during gametogenesis , embryogenesis and somatic tissue development . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Reprogramming of DNA methylation is an essential part of gametogenesis , and a role of two members of the DNA methyltransferase ( Dnmt ) family , Dnmt3a and Dnmt3L , has been recognized . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Recent data in mice indicate that Dnmt3a , an enzyme with de novo DNA methyltransferase activity , and the related protein Dnmt3L are required for methylation of imprinted loci in germ cells . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
In mouse , one of the two de novo DNA methyltransferases , Dnmt3a , and a related protein , Dnmt3L have been shown to be essential for imprint establishment in the parental germline . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Although the DNA methyltransferase 3 like ( Dnmt3L ) protein lacks DNA methylase activity , it is thought to establish the maternal imprint in combination with the functional DNA methyltransferases . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
Mammals have three Dnmt 3 genes ; Dnmt3a , Dnmt3b , and Dnmt3L , two of which encode active enzymes and one of which produces an inactive but necessary cofactor . ^^^
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
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Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA
Interacting proteins: Q9UJW3 and Q9UBC3	Pubmed	SVM Score :0.0
NA