Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.54612306
In total , 248 mice consisting of three different genotypes , + / + , + / and / , were obtained and DNA from their embryos was probed with two minisatellites , Pc 1 and Pc 2 . 0.54612306^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.81836422
UBL 1 was found to associate with human RAD51 / RAD52 proteins involved in DNA recombination and DNA double strand break repair ( 10 ) . 0.81836422^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.6109481
METHODS : Genomic DNA was extracted from whole blood by standard methods from a female patient with PC 1 and her parents , and from 50 normal , unrelated individuals . 0.6109481^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.69624309
Deletion analysis and colocalization experiment demonstrated that Dnmt3b interacts with SUMO 1 and Ubc 9 at its N terminal region . 0.69624309^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Mixing purified virion or PC 1 DNA polymerase with uninfected cells followed by fractionation did not produce the PC 2 form , suggesting that it is neither an artifact of purification nor the result of fortuitous complexing of reverse transcriptase with normal cellular component ( s ) . ^^^ Both PC 1 and PC 2 enzyme forms appeared antigenically similar to virion DNA polymerase , demonstrated identical divalent cation requirements for various template primers , and were capable of copying heteropolymeric regions of rabbit globin mRNA . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The XhoI SalGI fragment of the plasmid pCI DNA was inserted into the SalGI site of the cyanobacterium Anacystis nidulans R 2 integrative vector plasmid pIAH 4 . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Cell lysates from the plasmid clone pC 1 , containing a 4 . 8 kb EcoR 1 DNA fragment from Salmonella , caused elongation of Chinese hamster ovary ( CHO ) cells and this biologic activity was neutralized by anti CT . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Mutagenicity of quercetin was examined by means of DNA fingerprint analysis using the Pc 1 probe that efficiently detects mutations due to recombination . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
When analysed by DNA directed protein synthesis in vitro , both pC 1 and lambda B 8 DNA expressed two unique proteins ( 30 and 11 kDa ) similar to that of pJM17 . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
DNA DNA hybridization was not observed between plasmid pC 1 and genomic DNA from strains HL7b or HL7bR , nor between authentic naphthalene degradative genes ( plasmid NAH 2 ) and either plasmid pC 1 or strain HL7b , despite the observation that the degradative genes encoded on plasmid pC 1 functionally resembled broad specificity naphthalene degradative genes . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Proliferative cell indices ( PCI ) in 20 cases of endometrial adenocarcinoma were obtained by staining DNA polymerase alpha . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Almost all of the patients getting PCI also received lomustine , an agent associated with DNA repair inhibition and synergism with DNA damaging agents such as ionizing radiation or alkylating agents . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Restriction fragment length polymorphisms were detected in genomic DNA from mice bearing different PC 1 alleles . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
For the purpose of reasonable evaluation of the cell kinetics of RNA synthesis inhibitors , the flow cytometric technique using acridine orange staining for simultaneous measurement of cellular DNA and RNA content was applied in order to analyze the effects of actinomycin D ( ACT D ) on cultured cancer cells ( PC 1 ) . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
To investigate the possible mechanism of BLM resistance , these cell lines , namely HeLa , HeLa BLMr , HSG and MEC , were examined for 1 ) BLM permeability into cells by using 3H PEP which was a new derivative of BLM , 2 ) BLM inactivating activity in cell extracts by bioassay for antibacterial activity with B . subtilis PCI 219 strain , and 3 ) DNA repair activity after UV irradiation . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
These observations are consistent with previous observations of early responses of p 53 and PIC 1 to cellular DNA damage and subsequent G 1 cell cycle arrest or apoptosis . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Two distinct sequevars , denoted Pc 1 and Pc 2 , of the opportunistic pathogen Pneumocystis carinii have been previously identified based on the sequence of their 26S rRNA genes , the location of group 1 self splicing introns and pulsed field electrophoretic patterns of chromosomal DNA . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Furthermore , the common alpha 1 antitrypsin deficiency allele , PI * Z , has a unique DNA haplotype at the CBG , PIL , and PI loci , which extends over 60 kb in 97 % of cases and in 44 % of cases includes the PCI and AACT loci . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Low stringency screening of a rat hypothalamic complementary DNA library for additional members of the subtilisin like prohormone convertase ( PC ) family identified rat PACE 4 , which is 90 % identical to human PACE 4 in amino acid sequence , with much lower similarity to rat PC 1 , PC 2 , furin , PC 4 , or PC 6 . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The mutagenicity of quercetin , a flavonoid , was examined by means of DNA fingerprint analysis using the Pc 1 and Pc 2 minisatellite probes that efficiently detect mutations due to recombination . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The proliferating cell indexes ( PCI ) , determined by Ki 67 and anti DNA polymerase alpha monoclonal antibodies were higher and Ag NOR more numerous in medulloblastomas , glioblastomas , and metastatic carcinomas than in astrocytomas or meningiomas . ^^^ The Ki 67 and DNA polymerase alpha PCI correlated with the BrdU LI ( r = 0 . 87 and r = 0 . 84 , respectively ) and with each other ( r = 0 . 94 ) . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
DNA sequence analysis revealed that the lpcr gene in pc 1 y 7 cells contains a two nucleotide deletion within the fourth and fifth codons of the protochlorophyllide reductase precursor that causes a shift in the reading frame and results in premature termination of translation . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Frequencies for the PCI * B allele , determined from genomic DNA , differed among ethnic groups . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
When myr PCI ( Y ) was added to KMS 4 and KMS 8 cultures , it suppressed the production of inositol trisphosphate , DNA synthesis , and cell growth , all of which were induced by serum in both KMS 4 and KMS 8 cells . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
When myr PCI was added to KMS 4 and KMS 8 cells , both derived from familial adenomatous polyposis patients , it suppressed the production of inositol trisphosphate , DNA synthesis , and cell growth , all of which were induced by serum in both KMS 4 and KMS 8 cells . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NIH3T3 cells were cultured either with or without OA , subcloned and DNA from each clone was subjected to fingerprint analysis using the Pc 1 minisatellite probe . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
When myr PCI was added to KMS 8 cells , derived from familial adenomatous polyposis patients , it also suppressed the serum dependent IP 3 formation , DNA synthesis , and cell growth . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
After propagation of each subclone to 10 ( 7 ) to 10 ( 8 ) cells , DNA samples were digested with HinfI and analyzed by Southern blotting using the Pc 1 MS sequence as a probe . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The distal subcluster includes KAL , PCI , and AACT ; it occupies approximately 63 kb of DNA , and all three genes are transcribed in a proximal to distal orientation . ^^^ Within the subcluster , there is approximately 12 kb of intergenic DNA between KAL and PCI and approximately 19 kb between PCI and AACT . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Human PIC 1 ( HGMW approved symbol UBL 1 ) related sequences were isolated from human genomic DNA and were shown to represent processed pseudogenes . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Our recently described `` proliferation cell index ' ' ( PCI ) , based on nuclear immunoreactivity ( IR ) with the proliferation `` marker ' ' antigen Ki 67 , was significantly lower in those five MCCs of the classical `` DNA diploid ' ' type than in the seven `` DNA aneuploid ' ' ones . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The PCI results in various media correlated with corresponding DNA modification densities and were compared to statistical models incorporating DHBV characteristics and predictions of 8 MOP crosslink formation between DNA strands . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Drosophila SMT 3 genomic DNA was amplified by polymerase chain reaction , and its nucleotide sequence was found to be identical to that of the cDNA , indicating the absence of intron in its protein coding region . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Overexpression of UBL 1 down regulates DNA double strand break induced homologous recombination in CHO cells and reduces cellular resistance to ionizing radiation in HT 1080 cells . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Cells of different species and tissue origin transiently transfected in vitro with pCI / S or pDes / S plasmid DNA expressed readily detectable amounts of HBsAg , either intracellularly ( precipitated from cell lysates ) , or as secreted products ( detectable in ELISA ) . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
SUMO 1 conjugation to topoisomerase 1 : A possible repair response to topoisomerase mediated DNA damage . ^^^ In the current study , we show that treatment of mammalian cells or yeast cells expressing human DNA TOP 1 with camptothecin ( CPT ) induces covalent modification of the TOP 1 by SUMO 1 / Smt3p , a ubiquitin like protein . ^^^ This conclusion is based on the following observations : ( 1 ) Mammalian DNA TOP 1 conjugates induced by CPT were cross reactive with SUMO 1 / Smt3p specific antibodies both in yeast expressing human DNA TOP 1 as well as mammalian cells . ( 2 ) The formation of TOP 1 conjugates was shown to be dependent on UBC 9 , the E 2 enzyme for SUMO 1 / Smt3p . ( 3 ) TOP 1 physically interacts with UBC 9 . ( 4 ) Ubc 9 mutant yeast cells expressing human DNA TOP 1 was hypersensitive to CPT , suggesting that UBC9 / SUMO 1 may be involved in the repair of TOP 1 mediated DNA damage . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
As with c Jun , SUMO 1 modification of p 53 is abrogated by phosphorylation but remains unaltered upon chemical damage to DNA or Mdm 2 mediated ubiquitination . ^^^ The SUMO 1 attachment site in p 53 ( Lys 386 ) resides within a region known to regulate the DNA binding activity of the protein . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
SUMO 1 conjugation to human DNA topoisomerase 2 isozymes . ^^^ Surprisingly , ICRF 193 , which does not induce topoisomerase 2 mediated DNA damage but traps topoisomerase 2 into a circular clamp conformation , is also shown to induce similar SUMO 1 conjugation to topoisomerase 2 isozymes . ^^^ These studies raise the question on whether SUMO 1 conjugation to topoisomerases is an indirect result of a DNA damage response or a direct result because of protein conformational changes . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
From genomic DNA of Ralstonia pickettii isolate PIC 1 , a beta lactamase gene was cloned that encodes the oxacillinase OXA 22 . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Two hybrid screening in yeast with p73alpha isolated SUMO 1 ( small ubiquitin like modifier 1 ) , the enzyme responsible for its conjugation , Ubc 9 , and a number of novel SUMO 1 interacting proteins , including thymine DNA glycosylase , PM Scl 75 , PIASx , PKY , and CHD3 / ZFH . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
In this study , we used a recombinant vaccinia virus system to coexpress rat preproTRH complementary DNA with PC 1 , PC 2 , and the neuropeptide 7B2 in GH4C1 cells ( somatomammothophs , rat ) . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Properties and nucleotide sequence of mitochondrial plasmid like DNA pC 1 of cucumber ] . ^^^ Four kinds of plasmid like DNA , designated pC 1 , pC 2 , pC 3 and pC 4 were found in the mitochondrion of cucumber Jinyan No . 4 . ^^^ The circle plasmid like DNA pC 1 was cloned into the EcoR 1 site of pUC 19 using E . coli JM 109 as host . ^^^ The cloned pC 1 DNA was isolated and used as probes in Southern analyses of total mitochondrial DNA , nuclear DNA and chloroplast DNA . ^^^ Computer assisted searching the nucleotide sequence in GenBank database revealed pC 1 had no significant homology with known sequences of mitochondrial and plasmid like DNA , but had homology with the E . coli , Mycobaterium tuberculosis and Anacystis nidulans genomes . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Four kinds of mitochondrial plasmid like DNA , designated pC 1 , pC 2 , pC 3 and pC 4 , have been found in cucumber ( Jinyan No . 4 ) . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Neither AlPcS2a nor Photofrin affects the uptake of the transfecting DNA over the plasma membrane , therefore photochemical permeabilization of endocytic vesicles seems to be the most likely mechanism responsible for the positive PCI effect on gene transfection . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
We analyzed the binding capacity of topoisomerase 1 to 32P labeled plasmid pCI DNA , the immunoreactive topoisomerase 1 concentration and topoisomerase 1 activity in BC3H 1 myocytes and HL 60 cells treated with Hoechst 33342 and Hoechst 33258 by using covalent transfer of 32P radioactivity from plasmid DNA to topoisomerase 1 , Western blotting and topoisomerase 1 mediated plasmid relaxation assay , respectively . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Deletion experiments indicated that the region from amino acids 238 to 586 of BLM is required for the formation of dots / rod like structures associated with SUMO 1 , and the DNA helicase domain , but not the helicase activity itself , slightly affected the formation and / or stability of these structures . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Antibodies against SUMO 1 supershift the HSF 1 DNA binding complex , and modification of HSF 1 in a reconstituted SUMO 1 reaction system causes conversion of HSF 1 to the DNA binding form . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Sequence specificities for DNA binding were revealed and one base substitution and insertion into the Pc 1 repeat unit dramatically changed the affinity of each protein , suggesting that they bind to Pc 1 and Pc 1 like MNs in vivo . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
METHODS : DNA segment encoding env , tat and rev genes of HIV 1 subtype B ( strain BRU 2 ) was amplified and cloned into mammalian expression vector pCI to generate plasmid pCIBRU TRE . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
In the present paper , PCI is used in a pilot study to distinguish exon ( coding ) from intron ( noncoding ; interspersed within genes ) human DNA sequences . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The IL 2 cDNA of Xiaoshan chicken and the polyprotein gene of IBDV ZJ 2000 were amplified by RT PCR , cloned , sequenced and inserted into the control of CMV promoter and enhancer of pCI vector . 14 day old chickens were vaccinated intramuscularly with DNA vaccine , two weeks later , they were boosted with DNA , and two weeks post boost , they were challenged with virulent IBDV . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
We have taken advantage of the PCI strategy to enhance the delivery of a variety of macromolecules , including ribosome inactivating toxins , an immunotoxin , horse radish peroxidase , a ras peptide , RNA , oligonucleotides and protein encoding DNA , to the cytosol . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Recently , we have identified and purified minisatellite DNA binding proteins ( MNBPs ) that bind to the mouse hypervariable minisatellite Pc 1 , from NIH3T3 cells . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The SUMO 1 isopeptidase Smt 4 is linked to centromeric cohesion through SUMO 1 modification of DNA topoisomerase 2 . ^^^ We find that one function associated with the Smt3 / SUMO 1 isopeptidase Smt 4 is to control chromosome cohesion at centromeric regions and that a key Smt3 / SUMO 1 substrate underlying this function is Top 2 , DNA Topoisomerase 2 . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Other genes involved in the regulation of apoptosis , such as human inhibitor of apoptosis 1 ( HIAP 1 ) , Bax inhibitor 1 and MCL 1 , or DNA repair , such as poly ( ADP ribose ) polymerase 1 ( PARP 1 ) , 10 associated protein 1 ( XAP 1 ) , SUMO 1 ( sentrin 1 ) and RanGTPase activating protein 1 ( RanGAP 1 ) , were isolated . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Although three of the four genes encode proteins with PCI domains conserved in metazoan CSN proteins , their disruption does not confer the DNA damage sensitivity described in some fission yeast csn mutants . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Because DNA polymerase progression in vitro is blocked at the repeat , the characteristic intramolecular quadruplex structure of the repeat , at least in part , could be responsible for the hypermutable feature of Pc 1 and other MNs with similar repetitive units . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
SUMO 1 conjugation to intact DNA topoisomerase 1 amplifies cleavable complex formation induced by camptothecin . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
METHODS : PC 1 gene specific DNA sequence was used as probe to hybridize with total RNA from 10 pairs of tumor and normal tissues ; The C 4 2 cell genomic DNA was used as a template in the polymerase chain reaction to amplify PC 1 upstream regions from the translation initiation codon . ^^^ Our primary data shows that the highest promoter activity of PC 1 gene is within the 4939 bp DNA fragment and maybe there exists an enhancer element between the 1831 bp and 4939 bp area . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Among these are : ( 1 ) The efficiency of the technology with different gene therapy vectors ; ( 2 ) use of PCI with targeted vectors ; ( 3 ) the timing of DNA delivery relative to the photochemical treatment . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Although PIASy did not affect the interaction of Tcf 4 with beta catenin or DNA , Tcf 4 , SUMO 1 and PIASy were co localized in the nucleus and present in a complex in the PML body . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Photochemical internalisation ( PCI ) is a technology for achieving light induced release of DNA trapped inside these vesicles , and therefore represents a way of overcoming the endocytic membrane barrier and improving gene transfer . ^^^ The effect of PCI was highly dependent on the timing of illumination relative to the time of DNA delivery , both regarding the sequence of , and the time between , these two treatments . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The timing of the light treatment relative to the transfection period was also important , indicating that release of the DNA from early endosomes is important for the outcome of PCI induced transfection . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
PCI has been shown to potentiate the biological activity of a large variety of macromolecules and other molecules that do not readily penetrate the plasma membrane , including proteins , peptides , and DNA delivered as a complex with cationic polymers or incorporated in adenovirus . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The minisatellite DNA Pc 1 consists of tandem repeats of d ( GGCAG ) . ^^^ Here , we have demonstrated that hnRNP A 1 destroys the quadruplex of Pc 1 on binding and abrogates the arrest of DNA polymerase at the repeat . ^^^ Thus , hnRNP A 1 functions as if it is a chaperon to assist Pc 1 DNA to form the proper folding suitable for replication . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Genomic DNA was extracted from all patients , and the PC 1 K121Q polymorphism was determined by the PCR AvaII restriction enzyme . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The DNA topoisomerase 1 binding protein topors as a novel cellular target for SUMO 1 modification : characterization of domains necessary for subcellular localization and sumolation . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
A luciferase reporter gene assay suggested that SUMO 1 modification of this residue is required for transcriptional repression by PLZF , and an electrophoretic mobility shift assay showed that this modification increases the DNA binding activity of PLZF . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Modification of de novo DNA methyltransferase 3a ( Dnmt3a ) by SUMO 1 modulates its interaction with histone deacetylases ( HDACs ) and its capacity to repress transcription . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
These findings indicate that photochemical transfection allows : ( 1 ) selective enhancement in gene expression and gene mediated biological effects ( cell killing by the Hsvtk / GCV approach ) in response to illumination ; ( 2 ) the use of low , suboptimal for the nonviral transfection methods without PCI , doses of both DNA and the vector , which may be relevant and advantageous for therapeutic gene transfer in vivo . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
When the gnotobiotic rats were 28 days old they were injected with pGJA P , pGLUA P , fusion anticaries DNA vaccine against both PAc , cell surface protein antigen , and glucosyltransferase ( GTF ) , pCI or normal saline into the quadriceps muscle of thigh respectively , 2 weeks later a booster shot was given . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Its endogenous SUMOylation was demonstrated , and SUMOylation of PLAG 1 was further investigated in cells co transfected with PLAG 1 and SUMO 1 DNA or a SUMO 1 mutant form and similarly examined in the presence or absence of DNA encoding the various PIAS proteins . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Employing the powerful affinity purification of Protein A Smt 3 ( Smt 3 is the yeast homologue of SUMO 1 ) from yeast lysates in combination with tandem liquid chromatography mass spectrometry , we have isolated potential Smt 3 carrying substrate proteins involved in DNA replication and repair , chromatin remodeling , transcription activation , Pol 1 , Pol 2 , and Pol 3 transcription , 5 ' pre mRNA capping , 3 ' pre mRNA processing , proteasome function , and tubulin folding . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
PCI is an innovative procedure based on light induced delivery of macromolecules such as DNA , proteins and other therapeutic molecules from endocytic vesicles to the cytosol of target cells . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
By administrating different amounts of plasmid DNA , we confirmed that the pCI chIL 2 , but not the backbone plasmid pCI , contributes to increased immunoprotection of DNA vaccine against IBDV . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The p 53 mutated status of FaDu human pharynx carcinoma cell line was first assessed by DNA sequencing and the cells were transfected using tetraglucosylated polyethylenimine ( PEI Glu 4 ) in conjunction with photochemical internalisation ( PCI ) . ^^^ Wild type p 53 gene transfer using PEI Glu4 / DNA complexes and PCI , yields sustained transgene expression and induces cell death in p 53 mutated FaDu cells . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Noncovalent SUMO 1 binding activity of thymine DNA glycosylase ( TDG ) is required for its SUMO 1 modification and colocalization with the promyelocytic leukemia protein . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Treatment with 4 hydroxynonenal caused a near complete redistribution of sumo 1 and sumo 3 to different protein targets , which included chaperones , antioxidant , and DNA damage signaling proteins . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
A panel of three CpG oligodeoxynucleotides ( ODN ) and three eukaryotic expression vectors currently used in experimental DNA vaccines in pigs ( pcDNA 1 , pcDNA3 . 1 and pCI ) were screened for their immunostimulatory activity on porcine PBMC by evaluating in vitro the lymphocyte proliferative responses and cytokine profiles ( IL 1alpha , IL 2 , IL 4 , IL 6 , IL 10 , IFN gamma , TGF beta , TNF alpha ) . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
We find that SUMO 1 conjugation at hsTAF 5 interferes with binding of TFIID to promoter DNA , whereas modification of hsTAF 12 has no detectable effect on TFIID promoter binding activity . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
SUMO 1 conjugation to the C terminal K 330 of TDG modulates the DNA binding function of the N terminus to induce dissociation of the glycosylase from the AP site while it leaves the catalytic properties of base release in the active site pocket of the enzyme unaffected . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Crystal structure of thymine DNA glycosylase conjugated to SUMO 1 . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
DNA repair factor XPC is modified by SUMO 1 and ubiquitin following UV irradiation . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
To test the hypothesis that modulation of granulosa cell apoptosis changes SUMO 1 expression during granulosa cell differentiation in the mouse ovary , we demonstrate that progesterone receptor ( PR ) proteins are absent in pre ovulatory granulosa cell nuclei , whereas they are expressed in periovulatory granulosa cell nuclei in parallel with decreases in SUMO 1 expression , caspase 3 activation , and DNA fragmentation in vivo . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Three months after the last DNA immunization , three cows from each of the pGM CSF , internal ribosomal entry site ( IRES ) , pCTLA and pCI groups were injected with 200 microg of recombinant ClfA protein in incomplete Freund ' s adjuvant . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Human ( h ) DNA topoisomerase 1 has been identified as a major SUMO 1 target in camptothecin treated cells . ^^^ In response to TOP 1 mediated DNA damage induced by camptothecin , multiple SUMO 1 molecules are conjugated to the N terminal domain of a single TOP 1 molecule . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Specifically , restriction digests of genomic DNA samples from randomly selected , non transformed clones , isolated from cultures after several days exposure to promoters , were visualized by Southern hybridizations with the multi locus pentamer repeat sequence probe , Ms 6 Hm ( Pc 1 ) . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
In trial 2 , neutrophil counts were doubled to 66 % 1 hr after hemorrhage ( p < . 05 ) , colony forming units of microbes in the lung and liver were halved to 166+ / 56 and 134+ / 28 colony forming units ( p < . 05 for liver ) , and the macrophage inflammatory protein 2 expression in the lung was halved to 0 . 88+ / 0 . 06 pg of complementary DNA ( p < . 05 ) by G CSF prophylaxis compared with hemorrhage and PCI . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
In this review , we document the formation of DNA secondary structures by the G rich repetitive sequences that have been found in several minisatellites , telomeres and in various triplet repeats , and report their effects on in vitro DNA synthesis . d ( GGCAG ) repeats in the mouse minisatellite Pc 1 were demonstrated to form an intra molecular folded back quadruplex structure ( also called a G 4 ' structure ) by NMR and CD spectrum analyses . d ( TTAGGG ) telomere repeats and d ( CGG ) triplet repeats were also shown to form G 4 ' and other unspecified higher order structures , respectively . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
We could obtain a DNA construct containing SUMO 1 gene with His 6 tag sequence with high efficiency by the next day . . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Structure determination of the TDG central region conjugated to SUMO 1 previously suggested a mechanism in which the SUMOylation induced conformational change in the C terminal region of TDG releases TDG from tight binding to its product DNA . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
Modification of MBD 1 with either SUMO 2 / 3 or SUMO 1 facilitated the interaction between MBD 1 and MCAF 1 , suggesting that SUMOylation links the methylation of DNA and histones . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
The five ORFs , encoding a DNA recombinase , replication protein ( Rep ( pC 1 ) ) and three proteins of unknown function , were predicted on pC 1 . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
A new and a reclassified ICF patient without mutations in DNMT3B and its interacting proteins SUMO 1 and UBC 9 . ^^^ Similarly , the coding region of two DNMT3B interacting proteins , SUMO 1 and UBC 9 , did not reveal mutations . ^^^
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA
Interacting proteins: Q9UBC3 and P63165 Pubmed SVM Score :0.0
NA