| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| SWI 2 is believed to be the founding member of a new subfamily of DNA stimulated ATPases / DNA helicases that includes proteins that function in DNA repair ( RAD 5 , RAD 16 , ERCC 6 ) , recombination ( RAD 54 ) , transcription ( MOT 1 , ISWI , brm , BRG 1 , hBRM ) and cell cycle control ( STH 1 ) . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| The ATPase ISWI is a subunit of several distinct nucleosome remodeling complexes that increase the accessibility of DNA in chromatin . ^^^ The ATPase activity of ISWI was stimulated by nucleosomes but not by free DNA or free histones , indicating that ISWI recognizes a specific structural feature of nucleosomes . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| The assembly of core histones and DNA into periodic nucleosome arrays is mediated by ACF , an ISWI containing factor , and NAP 1 , a core histone chaperone , in an ATP dependent process . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Recent studies have shown that two nucleosome remodeling complexes , NURF and CHRAC , open chromatin for transcription and replication by using their common catalytic subunit , the nucleosomal ATPase ISWI , to increase the mobility of nucleosomes relative to DNA sequence . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| We find that the ability to distort DNA is shared by the yeast SWI / SNF complex , Xenopus Mi 2 complex , recombinant ISWI , and recombinant BRG 1 , suggesting that the generation of superhelical torsion represents a primary biomechanical activity shared by all Snf2p related ATPase motors . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| ISWI induces nucleosome sliding on nicked DNA . ^^^ It has been proposed that ISWI might loosen histone DNA interactions through twisting DNA . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| In the absence of the ISWI complexes , the deposition of histones onto DNA is apparently normal , but the spacing of nucleosomes is greatly disturbed . ^^^ Despite the poor spacing of nucleosomes , ISWI depletion has little effect on DNA replication , chromosome condensation or sister chromatid cohesion in the cell free extracts . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| The ATPase ISWI is the catalytic core of several nucleosome remodeling complexes , which are able to alter histone DNA interactions within nucleosomes such that the sliding of histone octamers on DNA is facilitated . ^^^ These residues have been shown earlier to contact nucleosomal DNA , suggesting that ISWI recognizes an ' epitope ' consisting of the DNA bound H 4 tail . ^^^ Consistent with this hypothesis , the ISWI ATPase is stimulated by isolated H 4 tail peptides ISWI only in the presence of DNA . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| These studies revealed DNA and ISWI binding regions that are important for the chromatin assembly and ATPase activities of ACF . ^^^ Thus , Acf 1 facilitates chromatin assembly via an N terminal DNA binding region with a WAC motif , a central ISWI binding segment with a DDT domain , and a C terminal region with an acidic stretch , a WAKZ motif , PHD fingers , and bromodomain . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Mapping of hRAD 21 , SNF2h and Mi 2 binding sites by chromatin immunoprecipitation experiments reveals the specific association of these three proteins with human DNA elements containing Alu sequences . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| The imitation switch ( ISWI ) complex from yeast containing the Isw 2 and Itc 1 proteins was shown to preferentially slide mononucleosomes with as little as 23 bp of linker DNA from the end to the center of DNA . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| An ACF 1 ISWI chromatin remodeling complex is required for DNA replication through heterochromatin . ^^^ In vivo depletion of SNF2H slows the progression of DNA replication throughout S phase , indicating a functional overlap with ACF 1 . ^^^ Our data suggest that an ACF 1 SNF2H complex is part of a dedicated mechanism that enables DNA replication through highly condensed regions of chromatin . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Their shared ATPase subunit ISWI binds DNA at the sites of entry into the nucleosome . ^^^ A prevalent model assumes that DNA distortions catalysed by ISWI are converted into relocation of DNA relative to a histone octamer . ^^^ We have now found that transient interaction of HMGB 1 with nucleosomal linker DNA overlapping ISWI binding sites enhances the ability of ACF to bind nucleosomal DNA and accelerates the sliding activity of limiting concentrations of remodelling factor . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Immunodepletion of ISWI has no effect on either sperm chromatin decondensation or the kinetics and efficiency of DNA replication . ^^^ From these results , we conclude that ISWI is not necessary for sperm chromatin decondensation and the accelerated rates of DNA replication that characterize early development . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Evidence for DNA translocation by the ISWI chromatin remodeling enzyme . ^^^ In combination , these observations suggest that ISWI is a 3 ' 5 ' strand specific , ATP dependent DNA translocase that may be capable of forcing DNA over the surface of nucleosomes . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Other ISWI containing complexes that have yet to be characterized indicate that much remains to be learnt about yeast ISWI itself and importantly , how the various forms cooperate with different classes of chromatin remodeling ATPase , complexes containing histone acetylases , deacetylases , methylases and both DNA and RNA polymerases . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Multiple roles for ISWI in transcription , chromosome organization and DNA replication . ^^^ Here we discuss recent progress toward understanding the biological functions of ISWI , with an emphasis on its roles in transcription , chromosome organization and DNA replication . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Endogenous hSNF2H is also associated with DNA methyltransferase activity . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Many of the coprecipitating cellular proteins are known members of large cellular complexes involved in ( 1 ) DNA replication or damage repair , including RPA and MSH 6 ; ( 2 ) nonhomologous and homologous recombination , including the catalytic subunit of the DNA dependent protein kinase , Ku 86 , and Rad 50 ; and ( 3 ) chromatin remodeling , including BRG 1 , BRM , hSNF2H , BAF 155 , mSin3a , and histone deacetylase 2 . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| The nucleosome remodeling factor NURF is a four subunit , ISWI containing chromatin remodeling complex that catalyzes nucleosome sliding in an ATP dependent fashion , thereby modulating the accessibility of the DNA . ^^^ NURF binds to two separate locations on the nucleosome : a continuous stretch of linker DNA up to the nucleosome entry site and a region asymmetrically surrounding the nucleosome dyad within the minor grooves , close to residues of the histone H 4 tail that have been implicated in the activation of ISWI activity . ^^^ These findings support a sliding model in which the position specific tethering of NURF forces a translocating ISWI ATPase to pump a DNA distortion over the histone octamer , thereby changing the translational position of the nucleosome . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Members of the ISWI family of chromatin remodeling factors hydrolyze ATP to reposition nucleosomes along DNA . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| Connecting ACF to histones may be prerequisite for efficient conversion of ATP dependent conformational changes of ISWI into translocation of DNA relative to the histones during nucleosome mobilization . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| We propose that the WSTF ISWI complex has a role in the maintenance of chromatin structures during DNA replication . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| The WICH complex , consisting of the ISWI type ATPase SNF2H and the Williams Syndrome Transcription Factor ( WSTF ) , binds to replication foci using PCNA , a key factor in DNA and chromatin replication and DNA repair , as an interaction platform . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| We conclude that an SNF2h HDAC1 / 2 complex coordinates G 1 specific chromatin remodeling and histone deacetylation with the DNA replication initiation process at OriP . . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| In contrast to ISWI , ACF interacts symmetrically with DNA entry sites of the nucleosome . ^^^ Two color fluorescence cross correlation spectroscopy measurements show that ACF can bind four DNA duplexes simultaneously in a complex that contains two Acf 1 and ISWI molecules . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| ISWI ATPases assemble chromatin and slide and space nucleosomes , making the chromatin template fluid and allowing appropriate regulation of events such as transcription , DNA replication , recombination and repair . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| DNMT3B also interacts with histone deacetylase 1 ( HDAC 1 ) , the co repressor SIN3A and the ATP dependent chromatin remodeling enzyme hSNF2H . ^^^ |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9UBC3 and O60264 |
Pubmed |
SVM Score :0.0 |
| NA |
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