| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.75865133 |
| Using an EH domain specific probe derived from the eps 15 cDNA , we cloned and characterized a cDNA encoding an EH containing protein with overall similarity to Eps 15 ; we designated this protein Eps15r ( for Eps 15 related ) . 0.75865133^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| EH is a recently identified protein protein interaction domain found in the signal transducers Eps 15 and Eps15R and several other proteins of yeast nematode . ^^^ We show that EH domains from Eps 15 and Eps15R bind in vitro to peptides containing an asparagine proline phenylalanine ( NPF ) motif . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Indirect evidence suggests that eps 15 and eps15R are involved in endocytosis . ^^^ Here we show that microinjection of antibodies against eps 15 and eps15R inhibits internalization of EGF and transferrin . ^^^ These results demonstrate that eps 15 and eps15R are essential components of the endocytic machinery . . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Eps15R is a tyrosine kinase substrate with characteristics of a docking protein possibly involved in coated pits mediated internalization . eps15R was identified because of its relatedness to eps 15 , a gene encoding a tyrosine kinase substrate bearing a novel protein protein interaction domain , called EH . ^^^ Finally , we demonstrate that a sizable fraction of eps15R exists in the cell as a complex with eps 15 and that its EH domains exhibit binding specificities that are partially distinct from those of eps 15 . ^^^ We propose that eps 15 and eps15R are multifunctional binding proteins that serve pleiotropic functions within the cell . . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate that Eps 15 and Eps15R , two EH containing proteins , synergize with Hrb and Hrbl to enhance the function of Rev in the export pathway . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Differential patterns of expression of Eps 15 and Eps15R during mouse embryogenesis . ^^^ Eps 15 and Eps15R are related tyrosine kinase substrates , which have been implicated in endocytosis and synaptic vesicle recycling . ^^^ We analyzed the expression of Eps 15 and Eps15R during murine development , at the time of active neurogenesis . ^^^ The most striking difference was at the level of subcellular localization , with Eps 15 present in the cytosol and on the plasma membrane , while Eps15R exhibited mainly a nuclear localization . ^^^ In the 12 . 5 days post coitum neuroepithelium , Eps 15 was expressed in the ventricular zone , which contains proliferating neuroblasts , whereas Eps15R was found only in postmitotic neurons . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| The SH 3 domain of Crk binds specifically to a conserved proline rich motif in Eps 15 and Eps15R . ^^^ We isolated Eps 15 , a substrate of the epidermal growth factor receptor ( EGFR ) tyrosine kinase , and Eps15R , a novel protein with high sequence homology to the carboxyl terminal domain of Eps 15 . ^^^ Eps 15 and Eps15R bound specifically to the amino terminal SH 3 domain of Crk and coprecipitated equivalently with both c Crk and 5 Crk from cell lysates . ^^^ The amino acid sequences of Eps 15 and Eps15R featured several proline rich regions as putative binding motifs for SH 3 domains . ^^^ In both Eps 15 and Eps15R , we identified one proline rich motif which accounts for their interaction with the Crk SH 3 domain . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Here we report that a short amino acid stretch at the carboxy termini of the monoubiquitinated endocytic proteins Eps 15 and eps15R is indispensable for their monoubiquitination . ^^^ We confirm this for the UIMs of eps 15 , eps15R , epsins and Hrs . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| A ubiquitin interacting motif ( UIM ) is essential for Eps 15 and Eps15R ubiquitination . ^^^ Eps 15 and its related partner Eps15R play a key role in clathrin mediated endocytosis of transmembrane receptors . ^^^ Using a mutational approach , we have found that the second ubiquitin interacting motif ( UIM ) of Eps 15 and Eps15R is essential for their ubiquitination . ^^^ Our data demonstrate that the UIM does not contain the ubiquitin acceptor site but functions as a recruitment site for the ubiquitination machinery leading to the monoubiquitination of both Eps 15 and Eps15R . . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Non clathrin internalization of ubiquitinated EGFR depends on its interaction with proteins harboring the Ub interacting motif , as shown through the ablation of three Ub interacting motif containing proteins , eps 15 , eps15R , and epsin . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Eps 15 and its related protein Eps15R are key components of the clathrin mediated endocytic pathway . ^^^ We report here that ubiquilin ( hPLIC 1 ) , a type 2 ubiquitin like protein containing a ubiquitin like domain ( UBL ) and a ubiquitin associated domain ( UBA ) , interacts with both Eps 15 and Eps15R . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Large scale RNA interference analysis of PKC dependent endocytosis of HA DAT revealed the essential and specific role of an E 3 ubiquitin ligase , Nedd 4 2 ( neural precursor cell expressed , developmentally downregulated 4 2 ) , as well as the involvement of adaptor proteins present in clathrin coated pits , such as epsin , Eps 15 ( epidermal growth factor pathway substrate clone 15 ) , and Eps15R ( Eps 15 related protein ) . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Stonin 2 , but not stonin 1 , interacts with the endocytic machinery proteins Eps 15 , Eps15R , and intersectin 1 . ^^^ These interactions occur via two NPF motifs in the proline rich domain of stonin 2 and Eps 15 homology domains of Eps 15 , Eps15R , and intersectin 1 . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| Differential nucleocytoplasmic trafficking between the related endocytic proteins Eps 15 and Eps15R . ^^^ Eps 15 and Eps15R are constitutive components of clathrin coated pits that are required for clathrin dependent endocytosis . ^^^ The most striking difference between these two related proteins is that Eps15R is also found in the nucleus , whereas Eps 15 is excluded from this compartment at steady state . ^^^ They also provide an explanation for the different nuclear localization of Eps 15 and Eps15R and further evidence for a possible nuclear function for Eps 15 protein family members . . ^^^ |
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| Interacting proteins: Q9UBC2 and P42566 |
Pubmed |
SVM Score :0.0 |
| The Eps 15 homology ( EH ) domain was originally identified as a motif present in three copies at the NH 2 termini of Eps 15 and of the related molecule Eps15R . ^^^ |
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