| Interacting proteins: Q9NZI6 and Q9NZI7 |
Pubmed |
SVM Score :0.0 |
| A yeast one hybrid screen of 2 . 4 10 10 ( 6 ) cDNA clones from human placental JEG 3 cells yielded two unique clones ; one is the previously described transcription factor LBP 1b , which is induced by HIV , type 1 infection of lymphocytes , and the other is a new factor , termed LBP 9 , that shares 83 % amino acid sequence identity with LBP 1b . ^^^ Reverse transcriptase polymerase chain reaction detected LBP 1b in human placental JEG 3 , adrenal NCI H295A , liver HepG 2 , cervical HeLa , and monkey kidney COS 1 cells , but LBP 9 was detected only in JEG 3 cells . ^^^ When the 155 / 131 fragment was linked to a minimal promoter , co expression of LBP 1b increased transcription 21 fold in a dose dependent fashion , but addition of LBP 9 suppressed the stimulatory effect of LBP 1b . ^^^ |
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| Interacting proteins: Q9NZI6 and Q9NZI7 |
Pubmed |
SVM Score :0.0 |
| Three mammalian genes , CP 2 , LBP 1a and LBP 9 have been previously identified as homologues of grainyhead . ^^^ We demonstrate that MGR and BOM are more closely related to grh , whereas CP 2 , LBP 1a and LBP 9 are descendants of the dCP 2 gene . ^^^ |
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| Interacting proteins: Q9NZI6 and Q9NZI7 |
Pubmed |
SVM Score :0.0 |
| In transient transfection assays , LBP 1b activated the 155 / 131 element whereas LBP 9 suppressed its LBP 1b stimulated expression . ^^^ To assess the roles of these factors on the intact P450scc gene , we stably expressed LBP 1b or LBP 9 in JEG 3 cells . ^^^ All cell lines stably expressing a fusion protein of LBP 1b and enhanced green fluorescent protein increased P450scc expression , but cell lines stably expressing LBP 9 fused to enhanced green fluorescent protein either increased or decreased P450scc expression . 8 Br cAMP induced endogenous LBP 9 , but not LBP 1b expression . ^^^ Glutathione S transferase pull down assays showed that LBP 1b and LBP 9 can dimerize with themselves and with each other ; LBP 1b residues 300 540 and LBP 9 residues 300 479 were required for dimer formation . ^^^ Glutathione S transferase pull down assays , bandshifts , and transient transfection assays showed that TReP 132 ( another factor that can bind to 155 / 131 ) does not interact with either LBP 1b or LBP 9 , or influence their ability to induce or suppress transcription from the 155 / 131 element . ^^^ |
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