| In dopaminergic neurons , PICK 1 colocalizes with the dopamine transporter ( DAT ) and forms a stable protein complex . ^^^ Coexpression of PICK 1 with DAT in mammalian cells and neurons in culture results in colocalization of the two proteins in a cluster pattern and an enhancement of DAT uptake activity through an increase in the number of plasma membrane DAT . ^^^ Deletion of the PDZ binding site at the carboxyl terminus of DAT abolishes its association with PICK 1 and impairs the localization of the transporter in neurons . ^^^ |