| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.56344208 |
| These results suggest that the interaction of GluR 2 with PICK 1 may play a regulatory role in the expression of LTD in the hippocampus . . 0.56344208^^^ |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.74322611 |
| Taken together , it appears that the PDZ domain of PICK 1 is less sensitive to mutations for PKCalpha when compared with GluR 2 binding . 0.74322611^^^ |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.87057309 |
| We found that two GluR 2 interacting proteins , the PDZ domain containing Protein interacting with C kinase ( PICK 1 ) and N ethylmaleimide sensitive fusion protein ( NSF ) , are specifically required for CARP . 0.87057309^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :1.0256991 |
| AMPAR surface expression involves interactions between the GluR 2 carboxy tail and various proteins including glutamate receptor interacting protein ( GRIP ) , AMPA receptor binding protein ( ABP ) , protein interacting with C kinase 1 ( PICK 1 ) , N ethyl maleimide sensitive fusion protein ( NSF ) . 1.0256991^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| The PDZ domain of PICK 1 is required for the interaction and the mutation of a single amino acid in this region ( Lys 27 to Glu ) prevents interaction between PICK 1 and GluR 2 in the yeast two hybrid assay . ^^^ Flag tagged PICK 1 is retained by a glutathione S transferase ( GST ) fusion of the C terminal of GluR 2 ( GST ct GluR 2 ; short splice variant ) but not by GST ct GluR 1 ( long splice variant ) . ^^^ Recombinant full length GluR 2 is coimmunoprecipitated with flag PICK 1 using an anti flag antibody and flag PICK 1 is coimmunoprecipitated with an N terminal directed anti GluR 2 antibody . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| We studied the role of PDZ proteins GRIP , ABP , and PICK 1 in GluR 2 AMPA receptor trafficking . ^^^ We conclude that the association of GluR 2 with ABP and / or GRIP but not PICK 1 is essential for maintaining the synaptic surface accumulation of the receptor , possibly by limiting its endocytotic rate . . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| Here we show that PKC phosphorylation of the AMPA receptor GluR 2 subunit differentially modulates its interaction with the PDZ domain containing proteins GRIP 1 and PICK 1 . ^^^ In vitro binding and coimmunoprecipitation studies show that phosphorylation of serine 880 within the GluR 2 PDZ ligand significantly decreases GluR 2 binding to GRIP 1 but not to PICK 1 . ^^^ Immunostaining of cultured hippocampal neurons demonstrates that the Ser 880 phosphorylated GluR 2 subunits are enriched and colocalized with PICK 1 in the dendrites , with very little staining observed at excitatory synapses . ^^^ Interestingly , PKC activation in neurons increases the Ser 880 phosphorylation of GluR 2 subunits and recruits PICK 1 to excitatory synapses . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| We investigated the role of PDZ proteins ( GRIP , ABP , and PICK 1 ) interacting with the C terminal GluR 2 by infusing a ct GluR 2 peptide ( `` pep 2 SVKI ' ' ) into CA 1 pyramidal neurons in hippocampal slices using whole cell recordings . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| The PICK 1 protein interacts in neurons with the AMPA type glutamate receptor subunit 2 ( GluR 2 ) and with several other membrane receptors via its single PDZ domain . ^^^ The formation of PICK 1 PKCalpha complexes is strongly induced by TPA , and PICK 1 PKCalpha complexes are cotargeted with PICK 1 GluR2 complexes to spines , where GluR 2 is found to be phosphorylated by PKC on serine 880 . ^^^ PICK 1 also reduces the plasma membrane levels of the GluR 2 subunit , consistent with a targeting function of PICK 1 and a PKC facilitated release of GluR 2 from the synaptic anchoring proteins ABP and GRIP . ^^^ This work indicates that PICK 1 functions as a targeting and transport protein that directs the activated form of PKCalpha to GluR 2 in spines , leading to the activity dependent release of GluR 2 from synaptic anchor proteins and the PICK 1 dependent transport of GluR 2 from the synaptic membrane . . ^^^ |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| Co immunoprecipitation using anti Pick 1 antibodies revealed that the overexpressed GluR 2 C terminal decoy indeed prevented Pick 1 from interacting with the endogenous GluR2 / 3 . ^^^ |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| NSF and PICK 1 bind the AMPAR GluR 2 subunit and are involved in trafficking of AMPARs . ^^^ Here , we show that GluR 2 , PICK 1 , NSF , and alpha / beta SNAPs form a complex in the presence of ATPgammaS . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| Here , we study the roles of proteins [ N ethylmaleimide sensitive fusion protein ( NSF ) , glutamate receptor AMPAR binding protein ( ABP ) interacting protein ( GRIP ) / ( ABP ) , and protein interacting with C kinase 1 ( PICK 1 ) that interact with the GluR 2 subunit in the control of the surface expression and cycling of AMPARs . ^^^ |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| The distribution of PICK 1 partially overlaps with PKCalpha and glutamate receptor subunits such as GluR 2 . ^^^ These data suggest that PICK 1 may function in the regulation of PKCalpha and GluR 2 localization in components of the rat auditory system , which may be a fundamental mechanism of synaptic transmission and / or plasticity . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| The glutamate receptor subunit 2 ( GluR 2 ) PDZ ligand domain can be phosphorylated at serine 880 ( S 880 ) , and this disrupts interactions with GRIP / ABP ( glutamate receptor interacting protein / AMPA binding protein ) but not with PICK 1 ( PKC interacting protein 1 ) . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| The GluR 2 subunit of the AMPA receptor binds to a number of intracellular adapter proteins including GRIP , PICK 1 and NSF , which may link the receptor to proteins with signalling , scaffolding and other roles . ^^^ We implicate for the first time a possible role for GRIP , PICK 1 and NSF in neuropathic sensitisation from experiments with cell permeable blocking peptides mimicking their GluR 2 interaction motifs and also demonstrate differential changes in expression of these proteins following peripheral nerve injury . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| The expression of other proteins , even those related to a specific AMPA subunit ( such as ABP and PICK 1 to GluR 2 and GluR 3 ) , often had different distributions , whereas others ( like NSF ) are ubiquitously distributed in the brain . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| The removal of AMPA receptors requires protein kinase C phosphorylation of the GluR 2 subunit within its carboxyl terminal PSD 95 / Discs Large / Zona Occludens 1 ( PDZ ) ligand and binding of the PDZ domain containing protein , PICK 1 . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| GluR 1 and ABP mRNA expression in the occipital cortex and GluR 2 , GluR 3 , SAP 97 , and PICK 1 expression in either cortical area were not significantly altered . ^^^ The data also demonstrated significant differences in the abundances of mRNAs encoding GluR 1 4 subunits ( GluR 2 > GluR 3 > GluR 1 > GluR 4 ) and of AMPAR binding proteins ( SAP 97 > PICK 1 > GRIP > ABP ) in both diagnostic groups . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| PICK 1 and ABP / GRIP bind to the AMPA receptor ( AMPAR ) GluR 2 subunit C terminus . ^^^ Transfer of the receptor from ABP / GRIP to PICK 1 , facilitated by GluR 2 S880 phosphorylation , may initiate receptor trafficking . ^^^ Binding of PKCalpha or GluR 2 to the PICK 1 PDZ domain disrupts the intramolecular interaction and facilitates the PICK 1 BAR domain association with ABP / GRIP . ^^^ Interference with the PICK 1 ABP / GRIP interaction impairs S 880 phosphorylation of GluR 2 by PKC and decreases the constitutive surface expression of GluR 2 , the NMDA induced endocytosis of GluR 2 , and recycling of internalized GluR 2 . ^^^ We suggest that the PICK 1 interaction with ABP / GRIP is a critical step in controlling GluR 2 trafficking . . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| PICK 1 binds the AMPAR GluR 2 subunit and is involved in AMPAR internalisation and LTD . ^^^ Deletion of an N terminal acidic domain in PICK 1 reduces its ability to bind Ca2+ , and renders the GluR 2 PICK1 interaction insensitive to Ca2+ . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| Interestingly , such LTP was not affected by a Ca2+ / calmodulin dependent protein kinase 2 inhibitor or a peptide binding to a protein interacting with C kinase 1 , but was blocked by a peptide binding to N ethylmaleimide sensitive factor , which specifically binds to GluR 2 . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| Targeted in vivo mutations of the AMPA receptor subunit GluR 2 and its interacting protein PICK 1 eliminate cerebellar long term depression . ^^^ Phosphorylation of the AMPA receptor subunit GluR 2 on serine 880 as well as interaction of GluR 2 with PICK 1 have been suggested to contribute to the endocytic removal of postsynaptic AMPA receptors during LTD . ^^^ Here , we show that targeted mutation of PICK 1 , the GluR 2 C terminal PDZ ligand , or the GluR 2 PKC phosphorylation site eliminates cerebellar LTD in mice . ^^^ These results demonstrate that PICK 1 GluR2 PDZ based interactions and GluR 2 phosphorylation are required for LTD expression in the cerebellum . . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| In this issue of Neuron , Steinberg et al . provide firm evidence that PICK 1 and the C terminus of GluR 2 are central to this process by analyzing three different transgenic mice . . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| The OGD induced switch in receptor subunit composition requires PKC activation , dissociation of GluR 2 from AMPA receptor binding protein , and association with protein interacting with C kinase 1 . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| PICK 1 ( protein interacting with C kinase 1 ) regulates the surface expression of the AMPA receptor ( AMPAR ) GluR 2 subunit , however , the functional consequences of this interaction are not well understood . ^^^ In contrast , blockade of interactions with GluR 2 at the N ethylmaleimide sensitive factor site did not cause a change in subunit composition , suggesting that the effects of PICK 1 are not simply a nonspecific consequence of removing AMPARs from the surface . ^^^ Immunocytochemical and biochemical analyses in dissociated cultured hippocampal neurons show that PICK 1 causes a decrease in endogenous GluR 2 surface expression but no change in GluR 1 surface levels . ^^^ To address the physiological role of PICK 1 , we virally expressed C terminal GluR 2 peptides . ^^^ These findings suggest that PICK 1 acts to downregulate the GluR 2 content of AMPARs at hippocampal CA 1 synapses , thereby increasing synaptic strength at resting membrane potentials . . ^^^ |
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| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42262 and Q9NRD5 |
Pubmed |
SVM Score :0.0 |
| NA |
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