| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.53092202 |
| Thus it appears that the interaction of CRP with GPVI is determined by a combination of affinity and avidity . 0.53092202^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.60087296 |
| In binding assays using soluble or cell expressed human GPVI , we observed that ( 1 ) collagen , and the GPVI specific ligands collagen related peptides ( CRP ) and convulxin , competed with one another for the binding to GPVI and ( 2 ) monoclonal antibodies directed against the extracellular part of the human receptor displayed selective inhibitory properties on GPVI interaction with its ligands . 0.60087296^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Here we show that collagen and a collagen related peptide ( CRP ) , which binds to GPVI but does not bind to the integrin alpha2beta1 , induced Btk tyrosine phosphorylation in platelets . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| A similar set of observations was made for a collagen related peptide ( CRP ) , which binds to GPVI but not to the integrin alpha2beta1 ( GPIa / IIa ) . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Human platelets were activated either by glycoprotein ( GP ) Ia / IIa agonist ( rhodocytin ) or by a GPVI agonist ( collagen related peptide , CRP ) , and the intracellular signal transduction pathways were compared in the presence of various inhibitors . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| We have previously reported that a triple helical , collagen related peptide ( CRP ; also known as CRP XL ) containing a glycine proline hydroxyproline ( GPP * ) repeat motif and cross linked through cysteine residues at its N terminus and C terminus is a powerful stimulus of platelet aggregation and secretion through the surface receptor glycoprotein 6 ( GPVI ) . ^^^ The monomeric peptides partly inhibit the release of [ 3H ] 5 HT by CRP , suggesting that they are partial agonists of the collagen receptor GPVI . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Cross linked or monomeric collagen related peptide ( CRP ) , Gly Cys Hyp ( Gly Pro Hyp ) 10 Gly Cys Hyp Gly was highly adhesive for platelets in a glycoprotein 6 ( GpVI ) dependent manner . ^^^ The procoagulant response elicited by binding to CRP was inhibited by anti GpVI Fab fragments , but not by impeding integrin alpha2beta1 mediated events . ^^^ This suggests that the triple helical Gly Pro Hyp repeat in CRP and analogous sequences in fibrillar collagen stimulate the procoagulant response of adherent platelets by acting as ligands for GpVI . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Our results show that collagen related peptide { CRP : [ GCP * ( GPP * ) ( 10 ) GCP * G ] ( n ) ; P * =hydroxyproline } , which is selective to GPVI , induces formation of phosphatidylinositol 3 , 4 , 5 trisphosphate [ PI ( 3 , 4 , 5 ) P ( 3 ) ] and phosphatidylinositol 3 , 4 bisphosphate [ PI ( 3 , 4 ) P ( 2 ) ] in platelets . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| In contrast , jararhagin and P1E6 had a small , almost negligible inhibitory effect against responses to the GPVI selective agonist collagen related peptide ( CRP ) and the G protein coupled receptor agonist thrombin . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| The Src homology ( SH ) 2 domain containing protein tyrosine phosphatase SHP 1 is tyrosine phosphorylated in platelets in response to the glycoprotein 6 ( GPVI ) selective agonist collagen related peptide ( CRP ) , collagen , and thrombin . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| In the present study , we demonstrate that convulxin is a considerably more powerful agonist than collagen or the GPVI selective collagen related peptide ( CRP ) . ^^^ These results show that the trimeric convulxin stimulates a much greater phosphorylation of the FcR gamma chain and subsequent downstream responses relative to CRP and collagen , presumably because of its ability to cause a greater degree of cross linking of GPVI . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| In contrast , platelet aggregation and tyrosine phosphorylation of these proteins were inhibited when mouse platelets were preincubated with JAQ 1 in the absence of cross linking and were subsequently stimulated with a collagen related peptide ( CRP ) that is specific for GPVI and low concentrations of collagen . ^^^ These observations suggest that a second activatory binding site , which is distinct from the CRP binding site on GPVI on mouse platelets , is occupied in the presence of high concentrations of collagen . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Activation of the collagen receptor glycoprotein 6 ( GPVI ) by a collagen related peptide ( CRP ) induces stimulation of platelets and megakaryocytes through the phosphatidylinositol ( PI ) 3 kinase dependent pathway leading to activation of Bruton tyrosine kinase ( Btk ) and phospholipase Cgamma 2 ( PLCgamma 2 ) . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Here , we studied the capacity of 2 GPVI ligands , collagen related peptide ( CRP ) and the snake venom protein convulxin ( CVX ) , to elicit the procoagulant platelet response . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Previously we have shown that LAT ( / ) platelets exhibit defective responses to the GPVI specific agonist , collagen related peptide ( CRP ) . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| We investigated the contribution of secondary G protein signaling to the mechanism of GPVI stimulated platelet aggregation using the GPVI selective agonists , convulxin and collagen related peptide ( CRP ) as well as collagen . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| The glycoprotein 6 ( GPVI ) specific agonist collagen related peptide ( CRP ) and thrombin stimulated tyrosine phosphorylation of Vav 1 but not Vav 2 in human platelets . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| The GPVI specific agonists convulxin and collagen related peptide ( CRP ) also stimulate weak aggregation in PLCgamma 2 / platelets , which is inhibited by wortmannin and PP 1 . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| A remarkable time dependent increase in GPVI surface expression was found by flow cytometry when platelets were activated by collagen related peptide ( CRP ) and convulxin . ^^^ The GPVI mediated activation of platelets by CRP or convulxin resulted in similar ultrastructural changes and an increased GPVI labelling density on the activated platelet surface , which was accompanied by a decreased interior expression . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Tec / platelets exhibit a minor reduction in aggregation to threshold concentrations of collagen or the GPVI specific agonist collagen related peptide ( CRP ) , whereas responses to higher concentrations are normal . ^^^ A residual GPVI signal exists in the Btk / / Tec / platelets as CRP synergizes with ADP to mediate aggregation . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| We show that Fps / Fes and Fer are expressed in human and mouse platelets , and are activated following stimulation with collagen and collagen related peptide ( CRP ) , suggesting a role in GPVI receptor signaling . ^^^ |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Both proteins bound specifically to collagen related peptide ( CRP ) , a GPVI specific ligand , but hD1D2 bound CRP more strongly than did mD1D2 . ^^^ The position of lysine 59 on the apical surface of GPVI suggests a mode of CRP binding analogous to that used by the related killer cell Ig like receptors to bind HLA . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| In line with this , aggregation is potentiated in response to the GpVI specific collagen related peptide ( CRP ) after a slight delay . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Amongst several monoclonal antibodies to the extracellular domain of human GPVI , one , 9O12 . 2 , was selected for its capacity to disrupt the interaction of GPVI with collagen in a purified system and to prevent the adhesion of cells expressing recombinant GPVI to collagen and collagen related peptides ( CRP ) . ^^^ While 9O12 . 2 IgGs induced platelet activation by a mechanism involving GPVI and Fc gamma RIIA , 9O12 . 2 Fab fragments completely blocked collagen induced platelet aggregation and secretion from 5 microg mL 1 and fully prevented CRP induced activation from 1 . 5 microg mL 1 . 9O12 . 2 Fabs also inhibited the procoagulant activity of collagen stimulated platelets and platelet adhesion to collagen in static conditions . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Mitochondrial injury induced by incubation of mouse platelets with carbonyl cyanide m chlorophenyl hydrazone ( CCCP ) severely affected the cells ' responses to collagen or the GPVI specific agonist , collagen related peptide ( CRP ) , but not to thrombin or the stable thromboxane A ( 2 ) analog U46619 . ^^^ Platelet stimulation with thrombin or CRP , however , resulted in marked metalloproteinase dependent shedding of GPIbalpha , but not GPVI suggesting that different metalloproteinases are involved in the regulation of the two receptors or , alternatively , an additional signal is required to render GPVI susceptible to cleavage . . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Recently , several different rat anti murine GPVI monoclonal antibodies , termed JAQs 1 , 2 , and 3 , were produced that had the unique property of `` immunodepleting ' ' GPVI from the murine platelet surface and rendering it unresponsive to collagen or GPVI specific agonists like convulxin or collagen related peptide ( CRP ) . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Here we observed that incubation of platelets with convulxin , collagen , or collagen related peptide ( CRP ) resulted in GPVI signaling dependent loss of surface GPVI and the appearance of an approximately 55 kDa soluble fragment of GPVI as revealed by immunoblotting . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| The alphaIIbbeta 3 antagonist lotrafiban potentiated CRP induced dense granule release , suggesting that alphaIIbbeta 3 outside in signalling may attenuate GPVI signals . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Thus , with regard to the different steps of adhesion , activation secretion or aggregation , patients ' platelet responses to collagen and to the GPVI specific agonists , collagen related peptide ( CRP ) or convulxin were null or dramatically impaired . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| METHODS : We assessed the effects of the three currently available GP IIb / IIIa antagonists abciximab , tirofiban and eptifibatide on platelet aggregation induced by various procoagulant and GP 6 related agonists , i . e . collagen related peptide ( CRP ) , convulxin and collagen fibrils , in PPACK anticoagulated platelet rich plasma . ^^^ RESULTS : At concentrations that equally inhibited 80 % of ADP induced maximal aggregation abciximab inhibited GP 6 mediated platelet responses to CRP or convulxin significantly more than the low molecular weight antagonists ( CRP : abciximab 75+ / 18 % , tirofiban 41+ / 7 % and eptifibatide 41+ / 6 % ; convulxin : abciximab 90+ / 6 % , tirofiban 64+ / 20 % , eptifibatide 61+ / 14 % , p < 0 . 01 for all ) . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Aggregation to threshold concentrations of the GPVI specific agonist , CRP , was partially reduced in p110delta ( / ) and p110delta ( D910A / D910A ) platelets . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| Using recombinant human glycoprotein 6 ( GPVI ) , we evaluated the effect of N linked glycosylation at the consensus site Asparagine 92 Glycine Serine 94 ( N92GS94 ) on binding of this platelet specific receptor to its ligands , human type 1 collagen , collagen related peptide ( CRP ) , and the snake venom C type lectin convulxin ( CVX ) . ^^^ In COS 7 cells transiently transfected with GPVI , deglycosylation with peptide N glycosidase F ( PNGase F ; specific for complex N linked glycans ) or tunicamycin decreases the molecular weight of GPVI and reduces transfected COS 7 cell binding to both CRP and CVX . ^^^ These findings indicate that N linked glycosylation at N 92 in human GPVI is not required for surface expression , but contributes to maximal adhesion to type 1 collagen , CRP and , to a lesser extent , CVX . . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : By site directed mutagenesis of recombinant receptor fragments , we have previously identified residue lysine 59 of the platelet collagen receptor glycoprotein 6 ( GPVI ) as being critical for its interaction with the synthetic ligand collagen related peptide ( CRP ) and the inhibitory phage antibody 10B12 . ^^^ RESULTS : Binding of recombinant GPVI to collagen , like CRP , was dramatically reduced by the mutation of residue lysine 59 to glutamate . ^^^ Remarkably , the mutation of residues arginine 60 in domain one and arginine 166 in domain two , individually to alanine , which had no significant affect on CRP binding , reduced binding of recombinant GPVI to collagen . ^^^ |
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| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P02741 and Q9HCN6 |
Pubmed |
SVM Score :0.0 |
| NA |
|