| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.52020787 |
| In this report , we demonstrate that human Cdt 1 is phosphorylated by cyclin A dependent kinases dependent on its cyclin binding motif . 0.52020787^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.85322302 |
| Here , we report that replication dependent proteolysis of Cdt 1 requires its interaction with proliferating cell nuclear antigen ( PCNA ) , a homotrimeric processivity factor for DNA polymerases . 0.85322302^^^ Cdt 1 binds to PCNA through a consensus PCNA interaction motif that is conserved in Cdt 1 of all metazoans , and removal of PCNA from egg extracts inhibits replication dependent Cdt 1 destruction . 0.61771848^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| The dup transcript is cell cycle regulated and is under the control of E2F and Cyclin E . ^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| We find that phosphorylation and degradation of Dup protein at G1 / S requires cyclin E / CDK2 . ^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| PCNA binds to Cdt 1 through the six conserved N terminal amino acids . ^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| Its control has been uncovered by the discovery of the CDKs ( cyclin dependent kinases ) as master regulators of the cell cycle and the initiator proteins of DNA replication , such as the Origin Recognition Complex ( ORC ) , Cdc6 / 18 , Cdt 1 and the MCM complex . ^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the replication initiation factors Cdt 1 and Cdc 6 along with cyclin A cdk 2 promotes rereplication in human cancer cells with inactive p 53 but not in cells with functional p 53 . ^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| We report here that overexpression of the proliferating cell nuclear antigen ( PCNA ) inhibitory domain from the CDK inhibitors p 21 and p 57 , but not the CDK cyclin inhibitory domain , blocked Cdt 1 degradation in cultured mammalian cells after UV irradiation . ^^^ In vivo soluble Cdt 1 and PCNA co elute by gel filtration and associate with each other physically . ^^^ Silencing PCNA in cultured mammalian cells or repression of pcn 1 expression in fission yeast blocked Cdt 1 degradation in response to DNA damage . ^^^ We suggest that the Cul 4 Ddb1 ligase evolved to ubiquitinate Cdt 1 during normal cell growth as well as in response to DNA damage and a separate E 3 ligase , possibly SCF ( Skp 2 ) , evolved to either share or take over the function of Cdt 1 ubiquitination during normal cell growth and that PCNA is involved in mediating Cdt 1 degradation by the Cul 4 Ddb1 ligase in response to DNA damage . . ^^^ An evolutionarily conserved function of proliferating cell nuclear antigen for Cdt 1 degradation by the Cul 4 Ddb1 ubiquitin ligase in response to DNA damage . ^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| PCNA is a cofactor for Cdt 1 degradation by CUL4 / DDB1 mediated N terminal ubiquitination . ^^^ Here we show that the N terminus of Cdt 1 contains a second degradation signal that is active after DNA damage and in S phase and is dependent on the interaction of Cdt 1 with proliferating cell nuclear antigen ( PCNA ) through a PCNA binding motif . ^^^ |
|
| Interacting proteins: Q9H211 and P12004 |
Pubmed |
SVM Score :0.0 |
| In Xenopus , Cdt 2 is recruited to replication forks via Cdt 1 and PCNA , where Cdt 1 ubiquitylation occurs . ^^^ |
|