| Coexpression of human Notch 1 ( hN 1 ) depleted WWP 1 from the nucleus to colocalise with hN 1 in early endosomes , dependent on the presence of the C terminal HECT domain . ^^^ To investigate the possibility that WWP 1 is also associated with Notch signalling we coexpressed human Notch 1 and WWP 1 in mouse myoblast cells . ^^^ Furthermore we found that full length expressed WWP 1 could interact in vitro with the cytoplasmic domain of human Notch 1 . ^^^ |