| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| Tetrameric oligomerization mediates transcriptional repression by the BRCA 1 dependent Kruppel associated box zinc finger protein ZBRK 1 . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| Functional dissection of transcription factor ZBRK 1 reveals zinc fingers with dual roles in DNA binding and BRCA 1 dependent transcriptional repression . ^^^ To clarify this issue , we have examined the functional interaction between BRCA 1 and ZBRK 1 , a BRCA 1 dependent KRAB eight zinc finger transcriptional repressor . ^^^ We report here the identification and molecular characterization of a portable BRCA 1 dependent transcriptional repression domain within ZBRK 1 composed of zinc fingers 5 8 along with sequences in the unique ZBRK 1 C terminus . ^^^ This C terminal repression domain functions in a BRCA 1 , histone deacetylase , and promoter specific manner and is thus functionally distinguishable from the N terminal KRAB repression domain in ZBRK 1 , which exhibits no BRCA 1 dependence and broad promoter specificity . ^^^ Significantly , we also find that the BRCA 1 dependent transcriptional repression domain on ZBRK 1 includes elements that modulate its sequence specific DNA binding activity . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| No significant correlation was found between altered BRCA 1 expression and the different types of ZBRK 1 mRNA expression . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| Sequence specific transcriptional corepressor function for BRCA 1 through a novel zinc finger protein , ZBRK 1 . ^^^ To explore the mechanistic basis for this regulation , a novel human gene , ZBRK 1 , which encodes a 60 kDa protein with an N terminal KRAB domain and eight central zinc fingers , was identified by virtue of its interaction with BRCA 1 in vitro and in vivo . ^^^ ZBRK 1 binds to a specific sequence , GGGxxx CAGxxxTTT , within GADD 45 intron 3 that supports the assembly of a nuclear complex minimally containing both ZBRK 1 and BRCA 1 . ^^^ ZBRK 1 represses transcription through this recognition sequence in a BRCA 1 dependent manner . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| A common DNA binding site for SZF 1 and the BRCA 1 associated zinc finger protein , ZBRK 1 . ^^^ Remarkably , this sequence is similar to the core DNA binding site described recently for ZBRK 1 , a KRAB ZFP that binds to BRCA 1 and is involved in coordinating the cellular DNA damage response . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| Two potential breast cancer susceptibility genes , encoding the BRCA 1 interacting proteins ZNF 350 ( or ZBRK 1 ) and BRIP 1 ( or BACH 1 ) , have been identified in yeast two hybrid screens . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| Previously , we identified a novel zinc finger protein , ZBRK 1 , which contains a KRAB domain at the N terminus , eight zinc fingers at the center , and a BRCA 1 binding region at the C terminus . ^^^ In a BRCA 1 dependent manner , ZBRK 1 represses Gadd45a transcription through binding to a specific sequence in intron 3 . ^^^ In both BRCA 1 proficient and deficient cells , ZBRK 1 is degraded with similar efficiencies independent of BRCA 1 E3 ligase activity . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| ZBRK 1 , KAP 1 , and the ZBRK 1 binding protein BRCA 1 were shown by indirect immunofluorescence to be present in replication compartments in lytically induced D 98 HR1 cells , and additionally , chromatin immunoprecipitation assays determined that these proteins associated with oriLyt DNA . ^^^ Exogenous ZBRK 1 , KAP 1 , or BRCA 1 increased the efficiency of oriLyt replication , while deletion of the ZBRK 1 binding site impaired replication . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| The GADD 45 , ZBRK 1 and BRCA 1 pathway : quantitative analysis of mRNA expression in colon carcinomas . ^^^ This gene is a target for coordinate regulation by both ZBRK 1 and BRCA 1 . ^^^ In this study , the relationships between GADD 45 , ZBRK 1 , and BRCA 1 expression were investigated in colon carcinomas . mRNA expression of these three genes was analysed in 116 colon carcinomas by real time reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^ Possible relationships between expression levels of GADD 45 , ZBRK 1 , and BRCA 1 , and a series of clinicopathological parameters classically associated with poor prognosis , were also examined . ^^^ ZBRK 1 showed a tendency towards underexpression , while GADD 45 and BRCA 1 were generally overexpressed . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| BRCA 1 exerts transcriptional repression through interaction with CtIP in the C terminal BRCT domain and ZBRK 1 in the central domain . ^^^ BRCA 1 , CtIP , and ZBRK 1 form a complex that coordinately represses ANG 1 expression via a ZBRK 1 recognition site in the ANG 1 promoter . ^^^ |
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| Interacting proteins: Q9GZX5 and P38398 |
Pubmed |
SVM Score :0.0 |
| Thus , 19 single nucleotide polymorphisms ( SNPs ) in eight genes involved in base excision repair ( XRCC 1 , APEX , POLD 1 ) , BRCA 1 protein interaction ( BRIP 1 , ZNF 350 , BRCA 2 ) , and growth regulation ( TGFss 1 , IGFBP 3 ) were evaluated . ^^^ |
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