| Interacting proteins: Q9H0C5 and Q9BX70 |
Pubmed |
SVM Score :0.0 |
| We have identified a paralogous BTBD 1 counterpart gene on chromosome 19 , BTBD 2 . ^^^ We show the existence of the murine BTBD 1 and BTBD 2 orthologous genes , as well as the partial rat and bovine homologs . . ^^^ |
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| Interacting proteins: Q9H0C5 and Q9BX70 |
Pubmed |
SVM Score :0.0 |
| Characterization of BTBD 1 and BTBD 2 , two similar BTB domain containing Kelch like proteins that interact with Topoisomerase 1 . ^^^ BACKGROUND : Two hybrid screening for proteins that interact with the core domain of human topoisomerase 1 identified two novel proteins , BTBD 1 and BTBD 2 , which share 80 % amino acid identities . ^^^ RESULTS : The interactions were confirmed by co precipitation assays demonstrating the physical interaction of BTBD 1 and BTBD 2 with 100 kDa topoisomerase 1 from HeLa cells . ^^^ Northern analysis revealed only a single sized mRNA for each BTBD 1 and BTBD 2 in all human tissues tested . ^^^ BTBD 1 and BTBD 2 are widely if not ubiquitously expressed in human tissues , and have two paralogs as well as putative orthologs in C . elegans and D . melanogaster . ^^^ |
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| Interacting proteins: Q9H0C5 and Q9BX70 |
Pubmed |
SVM Score :0.0 |
| BTBD 1 and BTBD 2 colocalize to cytoplasmic bodies with the RBCC / tripartite motif protein , TRIM5delta . ^^^ We previously identified BTBD 1 and BTBD 2 as novel topoisomerase 1 interacting proteins that share 80 % amino acid identity . ^^^ In a number of mouse and human cells , BTBD 1 and BTBD 2 ( BTBD1 / 2 ) colocalized to punctate or elongated cytoplasmic bodies ( < 5 microm long and several per cell ) that were larger and more elongated in cancer cell lines than in fibroblasts and myoblasts . ^^^ |
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