| Interacting proteins: Q9BRG1 and Q96H20 |
Pubmed |
SVM Score :0.0 |
| This study characterizes ESCRT 2 , a soluble approximately 155 kDa protein complex formed by the class E Vps proteins Vps 22 , Vps 25 , and Vps 36 . ^^^ |
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| Interacting proteins: Q9BRG1 and Q96H20 |
Pubmed |
SVM Score :0.0 |
| Here we report the crystal structure of the core of the yeast ESCRT 2 complex , which contains one molecule of the Vps protein Vps 22 , the carboxy terminal domain of Vps 36 and two molecules of Vps 25 , and has the shape of a capital letter ' Y ' . ^^^ Vps 22 and Vps 36 form nearly equivalent interactions with the two Vps 25 molecules at the centre of the ' Y ' . ^^^ |
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| Interacting proteins: Q9BRG1 and Q96H20 |
Pubmed |
SVM Score :0.0 |
| The 3 . 6 A structure of the yeast ESCRT 2 core presented here reveals a trilobal complex containing two copies of Vps 25 , one copy of Vps 22 , and the C terminal region of Vps 36 . ^^^ Two PPXY motifs from Vps 25 are involved in contacts with Vps 22 and Vps 36 , and their mutation leads to ESCRT 2 disruption . ^^^ |
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| Interacting proteins: Q9BRG1 and Q96H20 |
Pubmed |
SVM Score :0.0 |
| RNA polymerase 2 elongation factor ELL was recently purified from rat liver as a component of a multiprotein complex containing ELL and three ELL associated proteins ( EAPs ) of approximately 45 ( EAP 45 ) , approximately 30 ( EAP 30 ) , and approximately 20 ( EAP 20 ) kDa ( Shilatifard , A . ( 1998 ) J . ^^^ |
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| Interacting proteins: Q9BRG1 and Q96H20 |
Pubmed |
SVM Score :0.0 |
| Here , we report that the three subunits of human ESCRT 2 , EAP 20 , EAP 30 , and EAP 45 , have a number of properties in common with their yeast orthologs . ^^^ Specifically , EAP 45 bound ubiquitin via its N terminal GRAM like ubiquitin binding in EAP 45 ( GLUE ) domain , both EAP 45 and EAP 30 bound the C terminal domain of TSG101 / ESCRT 1 , and EAP 20 bound the N terminal half of CHMP6 / ESCRT 3 . ^^^ |
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| Interacting proteins: Q9BRG1 and Q96H20 |
Pubmed |
SVM Score :0.0 |
| Structural comparisons detect no conformational changes between unliganded Vps 25 and Vps 25 within the ESCRT 2 complex composed of two Vps 25 copies and one copy each of Vps 22 and Vps 36 12 . ^^^ |
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