| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.64537711 |
| In this study , the interactions between BAG 1 and Hsc 70 are characterized and compared with the analogous GrpE DnaK bacterial system . 0.64537711^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| We present evidence that the protein BAG 1 is a potential modulator of the molecular chaperones , Hsp 70 and Hsc 70 . ^^^ BAG 1 binds to the ATPase domain of Hsp 70 and Hsc 70 , without requirement for their carboxy terminal peptide binding domain , and can be co immunoprecipitated with Hsp / Hsc70 from cell lysates . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| GrpE like regulation of the hsc 70 chaperone by the anti apoptotic protein BAG 1 . ^^^ BAG 1 also interacts with several steroid hormone receptors which require the molecular chaperones Hsc 70 and Hsp 90 for activation . ^^^ Here we show that BAG 1 is a regulator of the Hsc 70 chaperone . ^^^ BAG 1 binds to the ATPase domain of Hsc 70 and , in cooperation with Hsp 40 , stimulates Hsc 70 ' s steady state ATP hydrolysis activity approximately 40 fold . ^^^ Similar to the action of the GrpE protein on bacterial Hsp 70 , BAG 1 accelerates the release of ADP from Hsc 70 . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| The modulation of the chaperone activity of the heat shock cognate Hsc 70 protein in mammalian cells involves cooperation with chaperone cofactors , such as Hsp 40 ; BAG 1 ; the Hsc 70 interacting protein , Hip ; and the Hsc 70 Hsp90 organizing protein , Hop . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| Regulation of the heat shock conjugate Hsc 70 in the mammalian cell : the characterization of the anti apoptotic protein BAG 1 provides novel insights . ^^^ Recent studies have now added another component to the list of Hsc 70 cofactors , the BAG 1 protein . ^^^ Initially identified as an anti apoptotic molecule and binding partner of the cell death inhibitor Bcl 2 , BAG 1 appears to fulfill its cellular function through a modulation of Hsc 70 ' s chaperone activity . ^^^ BAG 1 acts as a nucleotide exchange factor in the Hsc 70 ATPase cycle , thereby competing with the cofactor Hip which stabilizes the ADP bound state of Hsc 70 . ^^^ The functional characterization of BAG 1 thus reveals an unexpected versatility in the regulation of Hsc 70 and appears to provide a link between apoptosis and the cellular chaperone machinery . . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| All three isoforms of human BAG 1 ( BAG 1 , BAG 1M , and BAG 1L ) retained the ability to bind Hsc 70 . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| In contrast , the cofactor BAG 1 abrogates the ' holding ' function of the Hsc70 / Hsp40 system and blocks the action of Hip on Hsc 70 . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| BAG 1 binds the ATPase domains of Hsp 70 and Hsc 70 , modulating their chaperone activity and functioning as a competitive antagonist of the co chaperone Hip . ^^^ The human BAG 1 , BAG 2 , and BAG 3 proteins bind with high affinity ( KD congruent with 1 10 nM ) to the ATPase domain of Hsc 70 and inhibit its chaperone activity in a Hip repressible manner . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| The chaperone function of the mammalian 70 kDa heat shock proteins Hsc 70 and Hsp 70 is modulated by physical interactions with four previously identified chaperone cofactors : Hsp 40 , BAG 1 , the Hsc 70 interacting protein Hip , and the Hsc 70 Hsp90 organizing protein Hop . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| The BAG 1 protein modulates the chaperone activity of Hsc 70 and Hsp 70 in the mammalian cytosol and nucleus . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| Distinct isoforms of the cofactor BAG 1 differentially affect Hsc 70 chaperone function . ^^^ Our results reveal that the different amino termini of the distinct BAG 1 isoforms determine the outcome of an Hsc 70 mediated folding event , most likely by transiently interacting with the polypeptide substrate . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| Human DnaJ homologs dj 2 and dj 3 , and bag 1 are positive cochaperones of hsc 70 . ^^^ Thus , dj 2 and dj 3 are the partner DnaJs of hsc 70 within the cell , functionally similar and much more efficient than dj 1 , and bag 1 is a positive cochaperone of the hsc 70 dj2 and hsc 70 dj3 systems . . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| Structural analysis of BAG 1 cochaperone and its interactions with Hsc 70 heat shock protein . ^^^ In this study , a structure based approach was used to identify interacting residues in a BAG 1 Hsc 70 complex . ^^^ An Hsc 70 binding fragment of BAG 1 was shown by multidimensional NMR methods to consist of an antiparallel three helix bundle . ^^^ Structural predictions were confirmed by site directed mutagenesis of these residues , resulting in loss of binding of BAG 1 to Hsc 70 in vitro and in cells . ^^^ Molecular docking of BAG 1 to Hsc 70 and mutagenesis of Hsc 70 marked the molecular surface of the ATPase domain necessary for interaction with BAG 1 . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| Co chaperones Bag 1 , Hop and Hsp 40 regulate Hsc 70 and Hsp 90 interactions with wild type or mutant p 53 . ^^^ The anti apoptotic factor Bag 1 can dissociate Hsp 90 from a pre assembled complex wild type p 53 protein , but it can not dissociate a pre assembled p53R175H Hsp 40 Hsc 70 Hop Hsp 90 heterocomplex . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| The carboxyl terminal lobe of Hsc 70 ATPase domain is sufficient for binding to BAG 1 . ^^^ This result suggests that the stabilizing contacts for docking of BAG 1 to Hsc 70 are located in the C terminal lobe of the ATPase domain . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| RESULTS : By characterizing the chaperone cofactors BAG 1 and CHIP , we gained insight into the cooperation of the molecular chaperones Hsc 70 and Hsp 70 with the ubiquitin / proteasome system , a major system for protein degradation in eukaryotic cells . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| BAG 1 expression in human breast cancer : interrelationship between BAG 1 RNA , protein , HSC 70 expression and clinico pathological data . ^^^ Direct interaction with 70 kD heat shock proteins , HSC 70 and HSP 70 , may mediate the diverse functions of BAG 1 . ^^^ Immunohistochemistry was used to examine the expression of BAG 1 and HSC 70 in 160 cases of invasive breast cancer . ^^^ HSC 70 was also detected in the majority ( 97 % ) of cases , although expression was not correlated with BAG 1 levels , oestrogen receptor status or tumour grade . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| BAG 1 is a ubiquitin domain protein that links the molecular chaperones Hsc 70 and Hsp 70 to the proteasome . ^^^ During proteasomal sorting BAG 1 can cooperate with another co chaperone , the carboxyl terminus of Hsc 70 interacting protein CHIP . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| Accordingly , we examined injured brain tissue from adult patients ( n = 8 ) that underwent emergent surgical decompression after TBI , for alterations in the inducible 72 kDa heat shock protein ( Hsp 70 ) , the constitutive 73 kDa heat shock protein ( Hsc 70 ) , and isoforms of the chaperone cofactor BAG 1 . ^^^ Both Hsp 70 and Hsc 70 coimmunoprecipitated with the cofactor BAG 1 . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| Of particular relevance to tumour cells , BAG 1 interacts with the anti apoptotic BCL 2 protein , various nuclear hormone receptors and the 70 kDa heat shock proteins , Hsc 70 and Hsp 70 . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| The functional role of Hsc 70 in L topogenesis was confirmed through modulation of its in vivo activity by overexpressing its co chaperones Hip and Bag 1 . ^^^ Overexpression of the Hsc 70 stimulating molecule Hip led to increased entrapping of preS on the cytosolic ER face and hence to a decrease in preS posttranslocation , whereas the negative regulator Bag 1 had the opposite effects . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| To determine whether changes in the expression of proteinases , 73 kDa constitutive heat shock cognate protein ( Hsc 70 ) and stress inducible 72 kDa heat shock protein ( Hsp 70 ) ( Hsc / Hsp70 ) , and Bcl 2 associated gene product 1 ( BAG 1 ) contribute to the remodeling response of muscle tissue , tibialis anterior muscles of male Sprague Dawley rats were injected with 0 . 75 % bupivacaine and removed at 3 , 5 , 7 , 10 , 14 , 21 , or 35 days postinjection ( n = 5 7 / group ) . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| While a role for BAG 1 in regulating Hsc 70 chaperone activity is firmly established in vitro , physiological roles of this regulation remain obscure . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| BAG 1 a nucleotide exchange factor of Hsc 70 with multiple cellular functions . ^^^ In fact , BAG 1 functions as a nucleotide exchange factor of mammalian cytosolic Hsc 70 , thereby triggering substrate unloading from the chaperone . ^^^ |
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| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P11142 |
Pubmed |
SVM Score :0.0 |
| NA |
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