| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Cloning and functional analysis of BAG 1 : a novel Bcl 2 binding protein with anti cell death activity . ^^^ Using a protein interaction cloning technique , we identified cDNAs that encode a novel Bcl 2 binding protein , termed BAG 1 . ^^^ The BAG 1 protein shares no significant homology with Bcl 2 or other Bcl 2 family proteins , which can form homo and heterodimers . ^^^ In gene transfer experiments using a human lymphoid cell line , Jurkat , coexpression of BAG 1 and Bcl 2 provided markedly increased protection from cell death induced by several stimuli , including staurosporine , anti Fas antibody , and cytolytic T cells , relative to cells that contained gene transfer mediated elevations in either BAG 1 or Bcl 2 protein alone . ^^^ The findings indicate that bag 1 represents a new type of anti cell death gene and suggest that some routes of apoptosis induction previously ascribed to Bcl 2 independent pathways may instead reflect a need for the combination of Bcl 2 and BAG 1 . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bcl 2 interacting protein , BAG 1 , binds to and activates the kinase Raf 1 . ^^^ Previously we identified a Bcl 2 interacting protein BAG 1 that enhances the anti apoptotic effects of Bcl 2 . ^^^ Like BAG 1 , the serine / threonine protein kinase Raf 1 also can functionally cooperate with Bcl 2 in suppressing apoptosis . ^^^ These observations suggest that the Bcl 2 binding protein BAG 1 joins Ras and 14 3 3 proteins as potential activators of the kinase Raf 1 . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| These mutants also fail to bind to BAG 1 and Raf 1 , two proteins that we have shown can associate with protein complexes containing Bcl 2 and which cooperate with Bcl 2 to suppress cell death . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The IL 2 mediated induction of BAG 1 expression required the activation of tyrosine kinase ( s ) and was sensitive to rapamycin as the induction of bcl 2 expression was . ^^^ The signaling pathway for BAG 1 gene expression thus highly resembles that for bcl 2 gene expression , strongly suggesting that their induction shares the same signaling pathway . ^^^ Taken together , these studies demonstrate that the serine rich region of the IL 2R beta c chain mediates the coordinated expression of bcl 2 and BAG 1 genes , thereby contributing to suppression of apoptosis . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| This protein was identified as BAG 1 , a recently characterized Bcl 2 functional partner , which prolongs cell survival through unknown mechanisms . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Western blot for bcl 2 , FasL , and BAG 1 showed a significant decrease of bcl 2 product only in mdx mice after exercise . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Furthermore , it was found that the WT 1 ( KTS ) induced apoptosis could not be suppressed by coexpression of either the Mr 21 , 000 E1B , the Bcl 2 , or the BAG 1 protein . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The expression and function of the newly identified Bcl 2 and Raf 1 binding protein , Bag 1 , during the cytokine regulated growth of B and T cell lines was examined . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bcl 2 inhibits apoptosis from a variety of stimuli , and a Bcl 2 binding protein BAG 1 also functions in protection from apoptosis in concert with Bcl 2 . ^^^ Here , we provide evidence that prolonged cell survival introduced by overexpression of Bcl 2 or BAG 1 proteins strongly promotes experimental pulmonary metastasis of melanoma B 16 BL6 cells . ^^^ In murine melanoma cell line B 16 BL6 , gene transfer mediated expression of the Bcl 2 or BAG 1 led to prolonged cell survival against serum starved apoptosis in vitro . ^^^ The Bcl 2 expressing B 16 cells , B 16 Bcl 2 and the BAG 1 expressing B 16 cells , B 16 BAG 1 strongly enhanced pulmonary metastasis in allogenic BALB / c nude mice and whole lung weights were increased by 2 . 4 fold and 1 . 4 fold , respectively , compared with control transfectants , suggesting that Bcl 2 is a stronger positive modulator of metastasis . ^^^ These enhanced metastatic potentials in the B 16 Bcl 2 and the B 16 BAG 1 transfectants were well correlated with anti cell death activity against serum starvation and enhanced cell viability on limiting dilution . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The binding of BAG 1 to one of its known cellular targets , Bcl 2 , in cell lysates was found to be dependent on ATP , consistent with the possible involvement of Hsp / Hsc70 in complex formation . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The BAG 1 protein appears to inhibit cell death by binding to Bcl 2 , the Raf 1 protein kinase , and certain growth factor receptors , but the mechanism of inhibition remains enigmatic . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Cooperative interception of neuronal apoptosis by BCL 2 and BAG 1 expression : prevention of caspase activation and reduced production of reactive oxygen species . ^^^ Forced expression of either BCL 2 or the BCL 2 binding protein BAG 1 blocked NGF withdrawal induced apoptosis , activation of caspases , and ROS generation , showing that they function upstream of caspases . ^^^ Coexpression of BCL 2 and BAG 1 was more protective than expression of either protein alone . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The Bcl 2 oncoprotein is a key regulator of apoptosis and the Bag 1 protein interacts with Bcl 2 and cooperates with Bcl 2 to suppress apoptosis . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Anti apoptotic genes , bag 1 and bcl 2 , enabled hybridoma cells to survive under treatment for arresting cell cycle . ^^^ Hybridoma 2E3 O cells were transfected with bcl 2 alone or with bcl 2 and bag 1 in combination . ^^^ The bcl 2 / bag 1 transfectant survived maintaining viability above 75 % for almost 5 days when the cells were treated with excess ( 30 mM ) thymidine for arresting cell cycle , whereas the mock transfectant survived for only 2 days , and the bcl 2 alone transfectant lived for 4 days . ^^^ Owing to this extended viable culture period , the bcl 2 / bag 1 transfectant produced twofold amount of antibody in comparison with the mock transfectant in non proliferating state prepared by the excess thymidine treatment . ^^^ When their proliferation was arrested by serum limitation , the bcl 2 / bag 1 transfectant and the bcl 2 alone transfectant survived for 3 days maintaining viability above 75 % while the mock transfectant survived only 1 day . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The anti apoptotic proteins Bcl 2 , Bcl 10 ( L ) , Mcl 1 , and BAG 1 were present at immunodetectable levels in 7 , 10 , 10 , and 9 of the 11 lines . ^^^ Comparing these 11 cell lines among themselves revealed that steady state levels of Bcl 2 , Bcl 10 ( L ) , Mcl 1 , and BAG 1 were present at relatively higher levels in 4 , 6 , 5 , and 5 of the lines , respectively . ^^^ Bcl 2 and BAG 1 protein levels were positively correlated ( p = 0 . 03 ) . ^^^ Immunoblot analysis of primary adenocarcinomas revealed expression of the anti apoptotic proteins Bcl 2 , Bcl 10 ( L ) , Mcl 1 , and BAG 1 , as well as the pro apoptotic proteins Bax , Bak , and CPP 32 , in at least 2 of the 3 tumors examined . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The expression of several apoptosis regulating proteins , including the Bcl 2 family proteins Bcl 2 , Bcl XL , Mcl 1 , Bax , Bak , and BAD ; the Bcl 2 binding protein BAG 1 ; and the cell death protease Caspase 3 ( CPP 32 ) , was evaluated by immunoblotting using 58 peripheral blood B CLL specimens from previously untreated patients . ^^^ Expression of Bcl 2 , Mcl 1 , BAG 1 , Bax , Bak , and Caspase 3 was commonly found in circulating B CLL cells , whereas the Bcl XL and BAD proteins were not present . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Initially identified as an anti apoptotic molecule and binding partner of the cell death inhibitor Bcl 2 , BAG 1 appears to fulfill its cellular function through a modulation of Hsc 70 ' s chaperone activity . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bcl 2 and a Bcl 2 binding protein BAG 1 function in protection from apoptosis induced by a variety of stimuli . ^^^ Here , we provide evidence that prolonged cell survival introduced by overproduction of Bcl 2 or BAG 1 strongly enhances peritoneal dissemination of human gastric cancer MKN 74 cells . ^^^ Gene transfer mediated overexpression of Bcl 2 or BAG 1 led to prolonged cell survival of MKN 74 cells against serum starved apoptosis and anoikis . ^^^ When the viable transfectants were inoculated into the intraperitoneal cavity of BALB / c nude mice , the Bcl 2 expressing MKN 74 cells and the BAG 1 expressing MKN 74 cells exhibited strongly enhanced peritoneal dissemination in BALB / c nude mice and whole disseminated tumor weights were increased by 4 fold and 3 . 3 fold , respectively , compared with the control transfectants . ^^^ The enhanced peritoneal dissemination of MKN 74 Bcl 2 and MKN 74 BAG 1 transfectants correlated well with resistance to cell death induced by serum starvation and anoikis . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| In addition , RA induced suppression of Bcl 2 expression was abrogated by overexpression of BAG 1 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| On the other hand , the levels of apoptosis inhibiting proteins bcl 2 , bcl xL and BAG 1 increased progressively after immortalization and transformation . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 is a multifunctional protein that blocks apoptosis and interacts with several types of proteins , including Bcl 2 family proteins , the kinase Raf 1 , certain tyrosine kinase growth factor receptors , and steroid hormone receptors , possibly by virtue of its ability to regulate the Hsp70 / Hsc70 family of molecular chaperones . ^^^ In some cases , cytosolic BAG 1 immunostaining was clearly associated with organelles resembling mitochondria , consistent with the reported interaction of BAG 1 with Bcl 2 and related proteins . ^^^ Furthermore , experiments using a green fluorescence protein ( GFP ) BAG 1 fusion protein demonstrated that overexpression of Bcl 2 in cultured cells can cause intracellular redistribution of GFP BAG 1 , producing a membranous pattern typical of Bcl 2 family proteins . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Here , we report that the expression of bcl 2 , bcl xL , bcl xS , bax and bad mRNA as well as of Bcl 2 , Bax , Bcl XL , Bcl XS and Bag 1 proteins is not modulated in these two paradigms of neuronal cell death . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Moreover , a Bcl 2 binding protein , BAG 1 , was shown to not only bind Raf 1 , but also increase the activity of this kinase through a protein protein interaction . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 and Bcl 2 in IL 2 signaling . ^^^ In this context , the induction of anti apoptotic molecules such as Bcl 2 oncoprotein and BAG 1 protein seems to be a key event for the anti apoptotic function of cytokines . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 is a novel anti apoptotic protein isolated by its interaction with another anti apoptotic protein , Bcl 2 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Our data suggest that the resistance protein ( s ) are not Bcl 2 , Bcl 10 , Fap 1 or Bag 1 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 is a Hsp70 / Hsc70 binding protein that interacts with Bcl 2 , Raf 1 , steroid hormone receptors , Siah 1 , and hepatocyte growth factor ( HGF ) receptors , implying multiple functions for the BAG 1 protein . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the anti apoptotic bcl xL transcript and the pro apoptotic bax mRNA was also detected but no alterations in bcl 2 or bag 1 mRNA levels were observed . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Mammary gland tissue , similar to many other tissues , expresses a number of different Bcl 2 relatives including bcl 10 , bax , bak , bad , bcl w , bfl 1 , bcl 2 as well as the bcl 2 binding protein Bag 1 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Acute treatment of B6C3F1 mice with phenobarbital resulted in increased levels of bcl 2 and decreased levels of bax protein , while acute treatment with WY 14 , 643 resulted in increased bcl 2 and BAG 1 protein in the liver . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 is an antiapoptotic protein that binds to and enhances the antiapoptotic activity of Bcl 2 . ^^^ In this study , we examined the expression of BAG 1 RNA or protein isoforms and its interacting antiapoptotic proteins , Bcl 2 and BcI 10 ( L ) , in breast normal and tumor cell lines and tissues by Northern or Western blot analysis . ^^^ The expression of Bcl 2 and Bcl 10 ( L ) correlated with that of BAG 1 in breast normal and carcinoma cell lines but not tissues . ^^^ Our study suggests that BAG 1 isoforms may serve as a molecular marker , independent of Bcl 2 and Bcl 10 ( L ) , for human breast cancer . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 is an anti apoptotic protein that interacts with Bcl 2 , Bcl XL , Hsp70 / Hsc70 , Raf 1 and numerous hormone or growth factor receptors . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Expression of Bcl 2 and its homologues , Bcl xL , Bcl xS , Bax , Bad , Bak and Bag 1 , was detected in all NHL cases , with wide variations between histological subtypes and within each subtype . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 / HAP is a multifunctional protein which complexes with BCL 2 and steroid hormone receptors ( including the ER ) , and can suppress apoptosis and influence steroid hormone dependent transcription . ^^^ In addition to the prototypical 36 kDa BAG 1 isoform , breast cancer cells expressed higher molecular weight isoforms and , in contrast to BCL 2 , BAG 1 expression was independent of oestrogens . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Given its proposed involvement in multiple cell signaling events as a regulator of Raf 1 , Bcl 2 , or androgen receptor , we wondered whether Bag 1 functions in vivo as a negative regulator of Hsp 70 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| In this report , we summarize our attempts thus far to explore the expression of several Bcl 2 family proteins , caspase 3 , and BAG 1 in primary breast cancer specimens and breast cancer cell lines . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bag 1 and Bcl 2 gene transfer in malignant glioma : modulation of cell cycle regulation and apoptosis . ^^^ Bag 1 is a heat shock 70 kDa ( Hsp 70 ) binding protein that can collaborate with Bcl 2 in suppressing apoptosis under some conditions . ^^^ To examine the biological effects of Bag 1 in glioma cells , we expressed Bag 1 or Bcl 2 transgenes in 2 human malignant glioma cell lines , LN 18 and LN 229 . ^^^ Coexpressed Bcl 2 abrogated these effects of Bag 1 . ^^^ Bag 1 had no such effect , either in the absence or presence of Bcl 2 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Consistent with survival , the levels of both antiapoptotic proteins Bcl 2 and Bag 1 were maintained in Ba / F3 GHR cells , but not in parental Ba / F3 cells upon growth factor deprivation . ^^^ Together , our results provide evidence for a new GH mediated pathway that initiates a survival signal through activation of the transcription factor NF kappaB and sustained levels of the antiapoptotic proteins Bcl 2 and Bag 1 . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Synergistic effect of Bcl 2 and BAG 1 on the prevention of photoreceptor cell death . ^^^ BAG 1 has previously been shown to augment the inhibitory effect of Bcl 2 on programmed cell death in cultured cell systems . ^^^ This study was designed to determine whether the coexpression of BAG 1 and Bcl 2 in the photoreceptors of mice with an autosomal dominant form of retinitis pigmentosa ( RP ) would enhance the protective effect provided by Bcl 2 alone . ^^^ The BAG 1 transgenic mice were crossed to Bcl 2 transgenics to obtain animals that coexpress the two transgenes in photoreceptor cells . ^^^ BAG 1 / Bcl 2 animals were then crossed to an RP mouse model ( a transgenic line overexpressing the S334ter rhodopsin mutant ) to assess the effect of coexpression of BAG 1 and Bcl 2 on retinal degeneration . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Flavopiridol ( 0 . 1 mcmol / L ) and UCN 01 ( 1 mcmol / L ) also induced striking decreases in the levels of the antiapoptosis proteins Mcl 1 , 10 linked inhibitor of apoptosis ( XIAP ) , and BAG 1 in nearly all cases of B CLL and of Bcl 2 in approximately half of B CLL specimens evaluated . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Other members of the Bcl 2 family ( Bag 1 , Bak , Bax , and Bcl xL ) were not altered in expression . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| We studied the expression of several genes involved in differentiation , apoptosis and chemoresistance : ets 1 , bax , bcl 2 , bag 1 , bcl 10 , mdr 1 and mrp . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The steady state levels of bcl 2 and bag 1 mRNAs were low in PRL deprived Nb 2 cells , but were markedly increased by PRL or FGF 2 . bcl 2 expression was induced within 1 h of PRL or FGF 2 addition and continued to increase to a level 20 to 25 fold above the control level within 24 h . bag 1 expression also increased within 1 h after the addition of PRL or FGF 2 , was maximal within 8 h , and declined slowly thereafter . ^^^ Furthermore , coincubation of aminoguanidine ( NOS inhibitor ) with FGF 2 completely abrogated the protective effect of FGF 2 on bcl 2 and bag 1 mRNA levels in PRL deprived Nb 2 cells . ^^^ This antiapoptotic action of FGF 2 appears to be mediated by stimulation of eNOS expression , increased levels of cellular NO , and stimulation of expression of the antiapoptotic genes bcl 2 and bag 1 . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The expression of other antiapoptotic proteins ( Bcl 10 ( L ) , Bag 1 , A 1 , and Mcl 1 ) did not prevent Bcl 2 AS induced apoptosis . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Since the Bcl 2 family of proteins constitutes a critical checkpoint in apoptosis , acting upstream of the apoptotic machinery , we investigated the expression of six Bcl 2 homologs ( Bcl 2 , Bcl 10 ( L ) , Mcl 1 , Bax , Bak , Bad ) and one non homologous associated molecule ( Bag 1 ) . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 ( also known as RAP46 / HAP46 ) was originally identified as a 46 kDa protein that bound to and enhanced the anti apoptotic properties of Bcl 2 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Isolation of Bcl 2 binding proteins that exhibit homology with BAG 1 and suppressor of death domains protein . ^^^ In addition , several proteins that are not members of the Bcl 2 family can bind to Bcl 2 , including BAG 1 protein . ^^^ In this study , we screened for proteins that bind to Bcl 2 , and isolated two additional members of the BAG 1 protein family , BAG 3 and BAG 4 . ^^^ Together , our findings demonstrate that other members of the BAG 1 protein family , namely BAG 3 and BAG 4 / SODD , bind to Bcl 2 and provide a potential link between pathways regulated by Bcl 2 and pathways regulated by Hsp 70 , as well as TNFR1 . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Aloe emodin induced apoptosis of CH 27 cells involved modulation of the expression of Bcl 2 family proteins , such as BclX ( L ) , Bag 1 , and Bak , and was associated with the translocation of Bak and Bax from cytosolic to particulate fractions . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| In contrast , phosphorylation of Bcl 2 induced by microtubule targeting drugs did not alter its ability to associate with Bcl 2 ( homodimerization ) , Bax , BAG 1 , or other Bcl 2 binding proteins . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The predictive value of bcl 2 , bax , bcl xL , bag 1 , fas , and fasL for chemotherapy response in advanced breast cancer . ^^^ In 126 patients , histological blocks of primary tumors were available for immunohistochemical analysis of bax , bcl 2 , bcl xL , bag 1 , fas and fasL . ^^^ RESULTS : Of the investigated factors , bag 1 correlated positively with bax , bcl 2 , and fasL , and fasL correlated positively with fas and bax . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Our data demonstrated that Gyp induced apoptotic cell death was accompanied by up regulation of Bax , Bak and Bcl 10 ( L ) , and down regulation of Bcl 2 and Bad , while it had no effect on the level of Bag 1 protein . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 ( BCL 2 athanogene 1 ) , a multifunctional protein which associates with steroid hormone receptors ( including the oestrogen receptor ) and the anti apoptotic BCL 2 protein , regulates steroid hormone dependent transcription and apoptosis . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Intragraft expression of antiapoptotic Bcl 2 and Bag 1 was increased in Ad HO 1 treated orthotopic liver transplants , as compared with Ad beta Gal controls . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The clonogenic growth of breast cancer cell lines was inhibited by a ligand for PPARgamma ( troglitazone , TGZ ) combined with a ligand for either retinoid 10 receptor ( RXR ) ( LG 10069 ) ( 4 / 8 cell lines ) , RAR ( ATRA ) ( 5 / 8 cell lines ) or RAR / RXR and RXR / RXR ( 9 cis RA ) ( 5 / 8 cell lines ) independent of their expression of bcl 2 , bag 1 , ERalpha , and p 53 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bag 1 exerts powerful antiapoptotic effects by binding and stabilizing Bcl 2 and interacting with the tumor necrosis factor receptor type 1 induced death signal . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| These in vitro results suggest that distinct isoforms of BAG 1 have different anti apoptotic functions and their functions may be correlated to increased Bcl 2 expression . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Both BAG 1 and BAG 3 / CAIR 1 interact with Bcl 2 and have been shown to have a supra additive anti apoptotic effect with Bcl 2 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| These data implicate induction of the stress response and its modulation by the chaperone cofactor and Bcl 2 family member BAG 1 , after TBI in humans . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Down regulation of Bcl 2 interacting protein BAG 1 confers resistance to anti cancer drugs . ^^^ BAG 1 was originally identified as a binding partner of anti apoptotic factor Bcl 2 [ Takayama et al . , Cell 80 ( 1995 ) 279 284 ] . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Of particular relevance to tumour cells , BAG 1 interacts with the anti apoptotic BCL 2 protein , various nuclear hormone receptors and the 70 kDa heat shock proteins , Hsc 70 and Hsp 70 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| This screen revealed the elevated expression of BCL 2 , BCL 2A1 , BAG 1 and TACE upon stimulation with GM CSF . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Peripheral immune cells were investigated for apoptosis and for the expression of apoptosis regulatory genes CD 95 , CD 95 ligand , FLIP , Bcl 2 , Bcl 10 ( L ) , Bag 1 , and caspase 3 by quantitative real time PCR . ^^^ While Bcl 2 levels were permanently and Bag 1 levels transiently elevated upon therapy , other apoptosis regulatory genes revealed no alterations . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bag 1 expression in thyroid neoplasm : its correlation with Bcl 2 expression and carcinoma dedifferentiation . ^^^ Bag 1 is a recently identified bcl 2 related protein and is known to be linked to the biological aggressiveness of some carcinomas . ^^^ MATERIALS AND METHODS : We immunohistochemically investigated bag 1 and bcl 2 expression in various thyroid neoplasms using monoclonal antibodies . ^^^ Furthermore , in thyroid neoplasm , bag 1 expression was directly linked ( p < 0 . 0001 ) to bcl 2 expression . ^^^ CONCLUSION : These findings suggest that bag 1 may interact with bcl 2 to play an important role in thyroid neoplasm before anaplastic transformation , and the disruption of events mediated by bag 1 and bcl 2 may be a typical characteristic of undifferentiated carcinoma . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Four anti apoptotic proteins were altered 2 . 1 to+14 . 6 fold for Bcl 2 and BAG 1 isoform p 33 and p 29 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| It was revealed that LIGHT treatment resulted in down regulation of anti apoptosis Bcl 2 family member : Bcl 2 , Bcl 10 ( L ) , Bag 1 , and Mcl 1 ; up regulation of pro apoptosis Bcl 2 family member : Bak and Ser ( 112 ) phosphor Bad ; down regulation of pro apoptosis Bcl 2 member Bax ; the other pro apoptosis member Bid remains unaltered . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| With a serial of Western blot analysis , it is revealed that gossypol induced cell cycle arrest is involved in P 21 up regulation and cyclin D 1 down regulation ; gossypol induced apoptosis triggers down regulation of anti apoptosis Bcl 2 members : Bcl 10 ( L ) , Bag 1 and Mcl 1 , up regulation of pro apoptosis Bcl 2 member Bak , activation of caspase 3 , 6 , 7 , 8 , and 9 , up regulation of Apaf 1 , release of cytochrome c ( cyto c ) from mitochondria , and activation of both DFF 45 and PARP . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The present study was performed to clarify the clinical implications of BAG 1 , a bcl 2 binding protein in esophageal squamous cell carcinoma ( ESCC ) . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| To further understand such merosin driven survival signaling , we analyzed the expression of five Bcl 2 homologs ( Bcl 2 , Bcl 10 ( L ) , Bax , Bak , Bad ) and one non homologous associated molecule ( Bag 1 ) in normal and merosin deficient myotubes , with or without pharmacological inhibitors for Fyn and p 38 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| The expression of transcripts for anti apoptotic ( survivin , survivin deltaEx 3 , survivin 2B , galectin 3 , bag 1 and bcl 2 ) and pro apoptotic ( bax alpha ) genes , and for multiple drug resistance related protein 1 ( MRP 1 ) gene were investigated , using RT PCR , in 106 breast tumour biopsies . ^^^ Overall , survivin , survivin deltaEx 3 , survivin 2B , bcl 2 , bag 1 , galectin 3 , bax alpha and MRP 1 mRNAs were detected in 68 , 54 . 7 , 9 . 4 , 78 . 4 , 80 . 9 , 98 . 9 , 97 . 8 and 72 . 8 % , respectively , of tumour specimens . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 has been identified as a Bcl 2 binding protein that inhibits apoptosis , either alone or in co operation with Bcl 2 . ^^^ Whereas BAG 1 seemed to co operate with Bcl 2 to repress p 53 induced apoptosis , co expression of these proteins had no inhibitory effect on apoptin induced apoptosis . ^^^ Moreover , Bcl 2 , and to some extent also BAG 1 , paradoxically enhanced the apoptotic activity of apoptin . ^^^ These results demonstrate that p 53 and apoptin induce apoptosis through independent pathways , which are differentially regulated by BAG 1 and Bcl 2 . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Our experiments suggest that BAG 1 inhibits anti cancer drug induced apoptosis through apoptosis regulation pathways that may involve the mitochondrial Bcl 2 / Bax ratio , p 53 , and differential anti cancer drug mediated cytochrome c release . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Age related expression patterns of Bag 1 and Bcl 2 in growth plate and articular chondrocytes . ^^^ Bag 1 is a Bcl 2 binding protein that promotes cell survival , interacts with a diverse group of cellular proteins , and may integrate multiple pathways involved in controlling cell survival , growth , and phenotype . ^^^ In the growth plate , Bag 1 expression is prominent in the late proliferative and prehypertrophic chondrocytes , displaying a pattern similar to what has been reported for Bcl 2 . ^^^ Further , the expression of both Bcl 2 and Bag 1 declines with age in the articular cartilage . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : BAG 1 has anti apoptotic actions and is known to bind BCL 2 and steroid receptors . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Bag 1 expression correlated positively with apoptosis related parameters such as Bcl 2 expression ( P = . 001 ) and proliferation related parameters including Ki 67 , mean argyrophilic nucleolar organizer regions ( mAgNOR ) , and the percentage of AgNOR ( pAgNOR ) ( P = . 0117 , P = . 0257 , and P = . 0008 , respectively ) . ^^^ However , a combined score of Bcl 2 and Bag 1 expression was inversely associated with AI ( P = . 046 ) . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Expression of oncogene proteins Bag 1 , p 53 , and bcl 2 in endometrium adenocarcinoma with different rate of differentiation ] . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Expression levels of Rab 2 , a G protein , and Bag 1 , a Bcl 2 binding protein are controlled by withdrawal of nicotine from cultured pheochromocytoma PC 12 cells . ^^^ Considering the neuroprotective effect of nicotine , we also examined the level of Bag 1 protein , which is a binding protein for Bcl 2 , an anti apoptotic factor , and found a slight increase in the gene expression of Bag 1 following nicotine withdrawal . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| A series of microarray studies with validating criteria have recently revealed a common , novel target for the long term actions of the structurally highly dissimilar mood stabilizers lithium and valproate : BAG 1 [ BCL 2 ( B cell CLL / lymphoma 2 ) associated athanogene ] . ^^^ Because BAG 1 attenuates glucocorticoid receptor ( GR ) nuclear translocation , activates ERK ( extracellular signal regulated kinase ) MAP ( mitogen activated protein ) kinases , and potentiates anti apoptotic functions of BCL 2 , extensive additional studies were undertaken . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Hemagglutinating virus of Japan artificial viral envelope liposome mediated cotransfer of bag 1 and bcl 2 genes protects hepatic cells against ischemic injury through BAG 1 assisted preferential enhancement of bcl 2 protein expression . ^^^ In the present study , we introduced simultaneously both mouse Bcl 2 associated athanogene 1 ( m bag 1 ) and the h bcl 2 gene by the same HVJ AVE liposome transfection method , and found that I / R induced hepatic injuries such as release of hepatic marker enzymes into blood , cell morphological degeneration , and cellular DNA strand cleavage were suppressed more effectively than by transfection with either gene singly . ^^^ In addition , the h Bcl 2 expression level in the ischemic state , but not in the nonischemic state , was markedly higher in h bcl 2 / m bag 1 cotransfected liver than in h bcl 2 transfected liver . ^^^ In contrast , the m BAG 1 expression level in the ischemic state , but not in the nonischemic state , was only slightly higher in h bcl 2 / m bag 1 cotransfected liver than in m bag 1 transfected liver . ^^^ Thus , with dual gene cotransfer , coexistent Bcl 2 protein exerts no activity to assist a marked enhancement of BAG 1 protein , whereas the function of overexpressed BAG 1 as a Bcl 2 binding protein may lead to the enhancement of efficient expression of h Bcl 2 in I / R treated liver as compared with nonischemic liver , which results in repression of diverse I / R induced cell death symptoms , presumably through the formation of functional complexes of BAG 1 and Bcl 2 . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| It activates the ERK signaling pathway , stabilizes Bcl 2 and upregulates Hsp 70 and Hsp 27 as well as the Hsp 70 co chaperone Bag 1 . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| METHODS : Differential expression of apoptosis related genes ( e . g . apoptosis protease activating factor 1 [ Apaf 1 ] ; caspases [ casp ] 3 , 5 , 8 and 9 ; Bad , Bax , Bcl 2 , Bim , c myc , Bag 1 , as well as p 53 ) was assessed by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ RESULTS : Reverse transcription PCR revealed the detection of apoptosis related m RNAs as follows ( group A compared to group B ) : Apaf 1 0 % / 0 % ; Bcl 2 0 % / 35 % ; Bim 0 % / 9 % ; Bag 1 0 % / 9 % ; p 53 0 % / 4 % ; casp 3 11 % / 52 % ; casp 5 59 % / 48 % ; casp 8 44 % / 22 % ; casp 9 4 % / 9 % ; Bax 81 % / 52 % ; Bad 96 % / 56 % , and c myc 89 % / 96 % . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| We have established that Rb interacts with the anti apoptotic BAG 1 ( Bcl 2 associated athanogene 1 ) protein , and that a decrease in nuclear localization of BAG 1 is detectable when the interaction between Rb and BAG 1 is disrupted by expression of the E 7 viral oncoprotein . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Expression of the Bcl 2 family proteins , Bcl xL and Bag 1 , was increased in CCL 2 treated myocytes subjected to hypoxia . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| Bag 1 is a cochaperone for the heat shock protein Hsp 70 that interacts with C Raf , B Raf , Akt , Bcl 2 , steroid hormone receptors and other proteins . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| AIMS AND BACKGROUND : To evaluate the function and distribution of BAG 1 protein in hyperplastic and neoplastic prostate tissue and establish the relationship between this protein and BCL related proteins ( BCL 2 and BAX ) , androgen receptor ( AR ) expression and chromogranin A . ^^^ BAG 1 , BCL 2 , BAX , androgen receptor and chromogranin A immunostaining was performed by means of standard avidin biotin peroxidase methods . ^^^ In the carcinoma group there was a positive correlation ( Pearson ) between BCL 2 and cytoplasmic / nuclear BAG 1 . ^^^ BAG 1 showed the same specific basal cell localization as BCL 2 in hyperplastic and normal glands . ^^^ BAG 1 in association with BCL 2 inhibits apoptosis and may prolong the life of neoplastic cells and give them a chance to gain new oncogenic features in early carcinogenesis . . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| BAG 1 is a co chaperone that regulates the activity of Hsp 70 , Bcl 2 , Raf 1 , growth factor , and steroid receptors ( e . g . , the Androgen Receptor ) . ^^^ |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99933 and P10415 |
Pubmed |
SVM Score :0.0 |
| NA |
|