| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :1.1273677 |
| We further show that Cdc 6 physically associates with Cdt 1 via its N terminal noncatalytic domain , a region we had previously shown to be essential for Cdc 6 function . 1.1273677^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.77902718 |
| Recombinant Cdt 1 , added to egg extracts in G 2 , crosses the nuclear membrane , binds to chromatin , and relicenses the chromosome for new rounds of DNA synthesis in combination with chromatin bound Cdc 6 . 0.77902718^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Different from the other eukaryotes , Arabidopsis houses in its genome two putative homologs of ORC 1 , CDC 6 and CDT 1 . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Three additional factors the origin recognition complex , Cdc 6 and Cdt 1 are required for origin licensing . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Additionally , p16ink4a attenuated the levels of the assembly factors Cdt 1 and Cdc 6 . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| We show that the loading of Xenopus ORC onto chromatin is strongly stimulated by both ADP , ATP and ATP gamma S whilst the loading of Cdc 6 and Cdt 1 is stimulated only by ATP or ATP gamma S . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| It is recruited to chromatin by the origin recognition complex ( ORC ) , Cdc 6 , and Cdt 1 , and it is activated at the G ( 1 ) / S transition by Cdc 45 and the protein kinases Cdc 7 and Cdk 2 . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| In eukaryotes , the initiation of DNA replication involves the ordered assembly on chromatin of pre replicative complexes ( pre RCs ) , including the origin recognition complex ( ORC ) , Cdc 6 , Cdt 1 and the minichromosome maintenance proteins ( MCMs ) . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| During the G ( 1 ) phase of the cell cycle , a prereplication complex ( pre RC ) consisting of ORC , Cdc 6 , Cdt 1 , and MCM 2 7 is established at replication origins on the chromatin . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Regulation of CDC 6 , geminin , and CDT 1 in human cells that undergo polyploidization . ^^^ Cdc 6 , cdt 1 , and geminin expression was analyzed during differentiation of two human megakaryoblastic cell lines , HEL and K 562 , which respectively do and do not establish endoreplication cycles . ^^^ Our results show that both cdt 1 and cdc 6 are differentially regulated during megakaryocytic differentiation and suggest an active role of cdc 6 in endomitosis . . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Mini chromosome maintenance ( MCM ) 2 7 recruitment to origins in G 1 requires origin recognition complex ( ORC ) , Cdt 1 , and Cdc 6 , and activation at G1 / S requires MCM 10 and the protein kinases Cdc 7 and S Cdk , which together recruit Cdc 45 , a putative MCM 2 7 cofactor required for origin unwinding . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the replication initiation factors Cdt 1 and Cdc 6 along with cyclin A cdk 2 promotes rereplication in human cancer cells with inactive p 53 but not in cells with functional p 53 . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Once the origin recognition complex ( ORC ) binds to origins , CDC 6 and CDT 1 associate with ORC and promote loading of the MCM 2 7 proteins onto chromatin , generating the preRC . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| The current concept regarding cell cycle regulation of DNA replication is that Cdt 1 , together with origin recognition complex and CDC 6 proteins , constitutes the machinery that loads the minichromosome maintenance complex , a candidate replicative helicase , onto chromatin during the G ( 1 ) phase . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| The origin recognition complex ( ORC ) marks the position of replication origins in the genome and serves as the landing pad for the assembly of a multiprotein , pre replicative complex ( pre RC ) at the origins , consisting of ORC , cell division cycle 6 ( Cdc 6 ) , Cdc 10 dependent transcript ( Cdt 1 ) and mini chromosome maintenance ( MCM ) proteins . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| In addition , we demonstrate that the induction of rereplication is dependent on the replication initiation factors CDT 1 and CDC 6 , and independent of the functional status of p 53 . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| This process involves the sequential assembly of the Origin Recognition Complex ( ORC ) , Cdc 6 , Cdt 1 and the MiniChromosome Maintenance ( Mcm 2 7 ) proteins onto chromatin to license the origin for use in the subsequent S phase . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| In eukaryotic cells , the function of DNA replication licensing components ( Cdc 6 and Cdt 1 , among others ) is crucial for cell proliferation and genome stability . ^^^ We also show that CDC 6 and CDT 1 are key targets for the coordination of cell proliferation , differentiation , and development . ^^^ Indeed , altered CDT 1 or CDC 6 levels have cell type specific effects in developing Arabidopsis plants : in leaf cells competent to divide , cell proliferation is stimulated , whereas in cells programmed to undergo differentiation associated endoreplication rounds , extra endocycles are triggered . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Concurrent with cohesin binding , pre replication complexes ( pre RCs ) are assembled at origins of DNA replication through the sequential loading of the initiation factors ORC , Cdc 6 , Cdt 1 and MCM 2 7 ( the ' licensing ' reaction ) . ^^^ Here , we use Xenopus egg extracts to show that the recruitment of cohesins to chromosomes requires fully licensed chromatin and is dependent on ORC , Cdc 6 , Cdt 1 and MCM 2 7 , but is independent of Cdk 2 . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Overexpression of two key licensing factors , Cdc 6 and Cdt 1 , leads to overreplication and chromosomal instability ( CIN ) in lower eukaryotes and recently in human cell lines . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| We have investigated regulation of the origin licensing factors Cdc 6 , Cdt 1 , Mcm 2 and Geminin in human somatic and germ cells . ^^^ Cdc 6 and Cdt 1 play an essential role in DNA replication initiation by loading the Mcm 2 7 complex , which is required for unwinding the DNA helix , onto chromosomal origins . ^^^ Our studies demonstrate that Cdc 6 , Cdt 1 and Mcm 2 play a central role in coordinating growth during the proliferation differentiation switch in somatic self renewing systems and that Cdc 6 expression is rate limiting for acquisition of replication competence in primary oocytes . ^^^ In striking contrast , we show that proliferation control during male gametogenesis is not linked to Cdc 6 or Mcm 2 , but appears to be coordinated by the negative regulator Geminin with Cdt 1 becoming rate limiting in late prophase . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| In eukaryotes , prereplication complexes ( pre RCs ) containing ORC , Cdc 6 , Cdt 1 , and MCM 2 7 are assembled on chromatin in the G 1 phase . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| The MCM 2 MCM7 complex is an essential component of the prereplication complex ( pre RC ) , which is recruited by the cdc 6 and cdt 1 proteins to origins of DNA replication during G ( 1 ) phase . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Licensing for DNA replication requires a strict sequential assembly of Cdc 6 and Cdt 1 onto chromatin in Xenopus egg extracts . ^^^ Licensing assay and immunoblotting analyses indicated that Cdt 1 could only license DNA replication and load Mcm 2 7 onto DNA when it binds to chromatin that has already associated with Cdc 6 . ^^^ These results provide evidence supporting that Cdc 6 and Cdt 1 must bind to chromatin in a strict order for DNA licensing to occur . . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Early in eukaryotic cell cycle , a pre RC is assembled at each replication origin with ORC , Cdc 6 , Cdt 1 and Mcm 2 7 proteins to license the origin for use in the subsequent S phase . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| One such protein is Cdt 1 , which is recruited first to the origin of DNA replication followed by cell division cycle 6 ( Cdc 6 ) and mini chromosome maintenance proteins ( Mcms ) . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| It was previously found that overexpression of certain replication proteins such as Cdc 6 and Cdt 1 in fission yeast resulted in multiple rounds of DNA replication in the absence of mitosis . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| The resulting protein DNA assembly is called the prereplicative complex ( pre RC ) , and its formation requires the origin recognition complex ( ORC ) , Cdc 6 , Cdt 1 , and ATP . ^^^ Inhibiting Cdc 6 ATP hydrolysis stabilizes Cdt 1 on origin DNA and prevents Mcm 2 7 loading . ^^^ |
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| Interacting proteins: Q9H211 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Interestingly , DNA binding of ORC , CDC 6 , and CDT 1 was significantly stabilized in the presence of geminin , under which conditions approximately 10 20 molecules each of ORC and CDC 6 were bound . ^^^ However , upon shifting the temperature to 23 degrees C , most ORC , CDC 6 , and CDT 1 molecules were displaced from the DNA , leaving about one ORC molecule on the plasmid , whereas approximately 10 MCM 2 molecules were loaded onto each plasmid . ^^^ |
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