| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.91726052 |
| To further investigate the molecular mechanism of nucleotide binding function , we have demonstrated that the Cdc 6 protein associates with Orc 1 in vitro and in vivo . 0.91726052^^^ Our results suggest that a proper molecular interaction between Orc 1 and Cdc 6 depends on the functional ATP binding of Cdc 6 , which may be a prerequisite step to assemble the operational replicative complex at the G1 / S transition . . 0.63323253^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.64706075 |
| In particular CDC 6 was found to bind strongly to ORC 1 and ORC 2 , and to MCM 7 proteins . 0.64706075^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Moreover , we demonstrated that exogenous overexpression of either HsCdc 6 or HsOrc 1 failed to induce DNA synthesis unlike overexpression of E2F1 , even though E2F1 induced both Cdc 6 and Orc 1 , suggesting that E2F may regulate the expression of another gene ( s ) , besides Cdc 6 and Orc 1 , required for induction of cellular DNA synthesis in mammalian cells . . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| The mutant proteins retained the ability to bind HsOrc 1 and HsCdc 6 but displayed aberrant conformations in the presence of nucleotides . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Unlike cdc18+ , orp1+ is expressed constitutively during the cell cycle . cdc18+ , CDC 6 , and orp1+ belong to a family of related genes that also includes the gene ORC 1 , which encodes a subunit of the origin recognition complex ( ORC ) of S . cerevisiae . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Characterization of a novel CDC gene ( ORC 1 ) partly homologous to CDC 6 of Saccharomyces cerevisiae . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| In Xenopus egg extract , the proteins Orc 1 , Orc 2 , Cdc 6 , and Mcm 4 are underphosphorylated in interphase and hyperphosphorylated in metaphase extract . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Binding of mcm 4 to chromatin requires orc 1 and cdc 18 ( homologous to Cdc 6 in budding yeast ) . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| However , this absence of chromatin bound Cdc 6 and MCM proteins from quiescent cells appears not to be due to the absence of ORC subunits as murine homologues of Orc 1 and Orc 2 remain chromatin bound in quiescent cells . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| ORC 1 , ORC 4 and Cdc 6 were stable ( T1 / 2 > 2 h ) and associated with a chromatin containing fraction throughout the cell cycle . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| CDC 6 proteins were also found to assemble in nuclear foci on non chromatin nuclear structures , although their physical association with ORC 1 has been undetectable . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| In G ( 1 ) , the pre replicative complex contains CDC 6 , MCM 3 , ORC 1 and ORC 2 proteins ; of these , the post replicative complex in S phase contains only ORC 2 ; in M phase none of them are bound . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| These observations suggest that in Drosophila , ORC 1 regulates origin utilization much as does Cdc 6 in budding yeast . . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Further , we identify conserved sequence motifs within the origins that are recognized by a family of three Sulfolobus proteins that are homologous to the eukaryotic initiator proteins Orc 1 and Cdc 6 . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Different from the other eukaryotes , Arabidopsis houses in its genome two putative homologs of ORC 1 , CDC 6 and CDT 1 . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Archaeal cell division cycle protein 6 ( Cdc 6 ) / Origin Replication Complex subunit 1 ( Orc 1 ) proteins share sequence homology with eukaryotic DNA replication initiation factors but are also structurally similar to the bacterial initiator DnaA . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| The biological role of archaeal proteins , homologous to the eukaryal replication initiation factors of cell division control ( Cdc 6 ) and origin recognition complex ( ORC 1 ) , has not yet been clearly established . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Here we demonstrate cooperative binding of Saccharomyces cerevisiae Cdc 6 to ORC on DNA in an ATP dependent manner , which induces a change in the pattern of origin binding that requires the Orc 1 ATPase . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| We demonstrated the utility of this system by showing the functional importance of the ATPase domains of human Cdc 6 and Orc 1 and the dispensability of the N terminal segments of Orc 1 and Orc 2 in this assay . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| The binding of the prereplication complex proteins Orc 1 , Orc 2 , Mcm 3 , Mcm 7 , and Cdc 6 and the novel DNA unwinding element ( DUE ) binding protein DUE B to the endogenous human c myc replicator was studied by chromatin immunoprecipitation . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Using the yeast two hybrid system and immunoprecipitation , we report here that murine HP1alpha interacts with the preRC proteins ORC 1 , ORC 2 and CDC 6 . ^^^ Immunofluorescence staining and EGFP / DsRed fusion proteins revealed a colocalization of HP1alpha with ORC 1 , ORC 2 and CDC 6 in heterochromatin , supporting the notion that ORC and probably CDC 6 play an important role in murine HP1alpha function . ^^^ |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13415 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
|