| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting results demonstrate that mouse homologues of Mcm 2 , Mcm 5 , and Cdc 6 are displaced from chromatin in quiescent cells . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| Identification of Cdc 6 protein domains involved in interaction with Mcm 2 protein and Cdc 4 protein in budding yeast cells . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| In all eukaryotes studied , this entails loading by Cdc 6 of the Mcm 2 7 helicase next to the origin recognition complex ( ORC ) . ^^^ |
|
| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| Employing this protocol , analyses of chromatin bound MCM 2 , MCM 3 , Cdc 6 and cdk 2 suggests that the ' classical ' prereplication complex is already formed during hypoxia . . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| Down regulation of the critical DNA replication initiation factor Cdc 6 occurred early after stress concurring with p 53 induction , and was followed by a decrease in Mcm 2 levels . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| We have investigated regulation of the origin licensing factors Cdc 6 , Cdt 1 , Mcm 2 and Geminin in human somatic and germ cells . ^^^ Cdc 6 and Cdt 1 play an essential role in DNA replication initiation by loading the Mcm 2 7 complex , which is required for unwinding the DNA helix , onto chromosomal origins . ^^^ Our studies demonstrate that Cdc 6 , Cdt 1 and Mcm 2 play a central role in coordinating growth during the proliferation differentiation switch in somatic self renewing systems and that Cdc 6 expression is rate limiting for acquisition of replication competence in primary oocytes . ^^^ In striking contrast , we show that proliferation control during male gametogenesis is not linked to Cdc 6 or Mcm 2 , but appears to be coordinated by the negative regulator Geminin with Cdt 1 becoming rate limiting in late prophase . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| However , upon shifting the temperature to 23 degrees C , most ORC , CDC 6 , and CDT 1 molecules were displaced from the DNA , leaving about one ORC molecule on the plasmid , whereas approximately 10 MCM 2 molecules were loaded onto each plasmid . ^^^ These results suggest that an ORC CDC 6 CDT1 complex pre formed on DNA is directly involved in MCM loading and imply that each DNA bound ORC molecule loads only one or a few MCM 2 7 complexes during metazoan pre RC formation . . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| Cellular exposure to cytotoxic concentration of SN 38 , the active metabolite of irinotecan ( 0 . 1 microM ) alone and in combination with noncytotoxic concentration of MSC ( 10 microM ) did not result in additional enhancement of chk 2 phosphorylation and downregulation of specific DNA replication associated proteins , cdc 6 , MCM 2 , cdc25A , nor increase in PARP cleavage , caspase activation and the 30 300 kb DNA fragmentation induced by SN 38 treatment . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| Moreover , proteolytic destruction of DNA replication associated proteins cdc 6 , MCM 2 , and cdc25A may induce a DNA damage checkpoint response . ^^^ The observed down regulation of DNA replication proteins cdc 6 , MCM 2 , and cdc25A after exposure to SN 38 with MSC further indicates a relationship between drug response and DNA damage . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| Many genes whose expression is regulated by E2F family transcription factors , including CDK 2 , CCNE 1 , CDC 6 , CDC 2 , MCM 2 , were significantly down regulated at 24 hr post IR . ^^^ |
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| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| Cdc 6 , which normally accumulates as cells progress from quiescence into G ( 1 ) , is capable of inducing the binding of Mcm 2 to Cdt 1 when ectopically expressed in quiescent cells . ^^^ Cdt 1 activity is inhibited by the geminin protein , and we provide evidence that the mechanism of this inhibition involves blocking the binding of Cdt 1 to both Mcm 2 and Cdc 6 . ^^^ |
|
| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P49736 |
Pubmed |
SVM Score :0.0 |
| NA |
|