| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| A novel Mcm 1 dependent element in the SWI 4 , CLN 3 , CDC 6 , and CDC 47 promoters activates M / G1 specific transcription . ^^^ This element , which we call an ECB ( early cell cycle box ) , was first identified in the SWI 4 promoter , but it is also present in the promoter of a G 1 cyclin CLN 3 , as well as in the promoters of three DNA replication genes : CDC 6 , CDC 47 , and CDC 46 . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| In the absence of functional Cdc 6 , Clb / Cdc28 kinases were necessary and sufficient for efficient net nuclear export of a fusion protein between Mcm 7 and the green fluorescent protein ( Mcm 7 GFP ) , whereas inactivation of these kinases at the end of mitosis coincided with the net nuclear import of Mcm 7 GFP . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting results demonstrate that mouse homologues of Mcm 2 , Mcm 5 , and Cdc 6 are displaced from chromatin in quiescent cells . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Identification of Cdc 6 protein domains involved in interaction with Mcm 2 protein and Cdc 4 protein in budding yeast cells . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Using DNase 1 footprinting in vitro , we have observed protection of the ECB elements from the CLN 3 , SWI 4 , CDC 6 , and CDC 47 promoters , which includes protection of the 16 bp palindrome to which Mcm 1 dimers are known to bind as well as protection of extended flanking sequences . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| In all eukaryotes studied , this entails loading by Cdc 6 of the Mcm 2 7 helicase next to the origin recognition complex ( ORC ) . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| In particular CDC 6 was found to bind strongly to ORC 1 and ORC 2 , and to MCM 7 proteins . ^^^ |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Indeed , Mcm 7 stimulates Mcm 1 binding to the early cell cycle box upstream of the promoters of MCM 7 as well as CDC 6 and MCM 5 . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Employing this protocol , analyses of chromatin bound MCM 2 , MCM 3 , Cdc 6 and cdk 2 suggests that the ' classical ' prereplication complex is already formed during hypoxia . . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Cdc 6 , which normally accumulates as cells progress from quiescence into G ( 1 ) , is capable of inducing the binding of Mcm 2 to Cdt 1 when ectopically expressed in quiescent cells . ^^^ Cdt 1 activity is inhibited by the geminin protein , and we provide evidence that the mechanism of this inhibition involves blocking the binding of Cdt 1 to both Mcm 2 and Cdc 6 . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| MCM 7 and its clamp loading partner Cdc 6 are highly specifically colocalized by ChIP and re ChIP in G ( 1 ) and early S on a 198 bp segment located near the center of the initiation zone . ^^^ |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| We find that the pre replication complex ( pre RC ) components Cdc 6 and Mcm 7 associate with ARS 1 in the mcm 10 1 mutant , suggesting that establishment of the pre RC is not compromised in this mutant . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Here , we sought to identify factors that associate with TR by using a TR DNA column and found that poly ( ADP ribose ) polymerase 1 ( PARP 1 ) and known replication factors , including ORC 2 , CDC 6 , and Mcm 7 , bound to TR . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Down regulation of the critical DNA replication initiation factor Cdc 6 occurred early after stress concurring with p 53 induction , and was followed by a decrease in Mcm 2 levels . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| We have investigated regulation of the origin licensing factors Cdc 6 , Cdt 1 , Mcm 2 and Geminin in human somatic and germ cells . ^^^ Cdc 6 and Cdt 1 play an essential role in DNA replication initiation by loading the Mcm 2 7 complex , which is required for unwinding the DNA helix , onto chromosomal origins . ^^^ Our studies demonstrate that Cdc 6 , Cdt 1 and Mcm 2 play a central role in coordinating growth during the proliferation differentiation switch in somatic self renewing systems and that Cdc 6 expression is rate limiting for acquisition of replication competence in primary oocytes . ^^^ In striking contrast , we show that proliferation control during male gametogenesis is not linked to Cdc 6 or Mcm 2 , but appears to be coordinated by the negative regulator Geminin with Cdt 1 becoming rate limiting in late prophase . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| However , upon shifting the temperature to 23 degrees C , most ORC , CDC 6 , and CDT 1 molecules were displaced from the DNA , leaving about one ORC molecule on the plasmid , whereas approximately 10 MCM 2 molecules were loaded onto each plasmid . ^^^ These results suggest that an ORC CDC 6 CDT1 complex pre formed on DNA is directly involved in MCM loading and imply that each DNA bound ORC molecule loads only one or a few MCM 2 7 complexes during metazoan pre RC formation . . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Cellular exposure to cytotoxic concentration of SN 38 , the active metabolite of irinotecan ( 0 . 1 microM ) alone and in combination with noncytotoxic concentration of MSC ( 10 microM ) did not result in additional enhancement of chk 2 phosphorylation and downregulation of specific DNA replication associated proteins , cdc 6 , MCM 2 , cdc25A , nor increase in PARP cleavage , caspase activation and the 30 300 kb DNA fragmentation induced by SN 38 treatment . ^^^ |
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| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Moreover , proteolytic destruction of DNA replication associated proteins cdc 6 , MCM 2 , and cdc25A may induce a DNA damage checkpoint response . ^^^ The observed down regulation of DNA replication proteins cdc 6 , MCM 2 , and cdc25A after exposure to SN 38 with MSC further indicates a relationship between drug response and DNA damage . ^^^ |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| Many genes whose expression is regulated by E2F family transcription factors , including CDK 2 , CCNE 1 , CDC 6 , CDC 2 , MCM 2 , were significantly down regulated at 24 hr post IR . ^^^ |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| RNA interference mediated depletion and chromatin immunoprecipitation assays show that endogenous ACTR directly controls the expression of genes important for initiation of DNA replication , which include cdc 6 , cdc25A , MCM 7 , cyclin E , and Cdk 2 . ^^^ |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| The binding of the prereplication complex proteins Orc 1 , Orc 2 , Mcm 3 , Mcm 7 , and Cdc 6 and the novel DNA unwinding element ( DUE ) binding protein DUE B to the endogenous human c myc replicator was studied by chromatin immunoprecipitation . ^^^ |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P33993 |
Pubmed |
SVM Score :0.0 |
| NA |
|