| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.73775496 |
| Cdc 6 is known to be phosphorylated by cyclin A cyclin dependent kinase 2 ( Cdk 2 ) , an event that promotes its exit from the nucleus and probably blocks it from initiating inappropriate DNA replication . 0.73775496^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| HsCdc 6 is an excellent substrate for Cdk 2 in vitro and is phosphorylated in vivo at three sites ( Ser 54 , Ser 74 , and Ser 106 ) that are phosphorylated by Cdk 2 in vitro , strongly suggesting that HsCdc 6 is an in vivo Cdk substrate . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc 6 , but dependent on cdk 2 . ^^^ Using Xenopus laevis egg extracts , we find that after the formation of these complexes and before initiation of DNA replication , cdc 6 is rapidly removed from chromatin , possibly degraded by a cdk 2 activated , ubiquitin dependent proteolytic pathway . ^^^ Importantly , under conditions in which both ORC and cdc 6 protein are absent from preinitiation complexes , DNA replication is still dependent on cdk 2 activity . ^^^ Therefore , the final steps in the process leading to initiation of DNA replication during S phase of the cell cycle are independent of ORC and cdc 6 proteins , but dependent on cdk 2 activity . . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| The transcription of a subset of these genes , which includes Cdc 6 , cyclin E , and cdk 2 , is also regulated during the cell cycle . ^^^ |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| In the first phase , the origin recognition complex and Cdc 6 prereplication proteins , but not the minichromosome maintenance complex , are necessary and biochemically sufficient for ATP dependent binding of cyclin E Cdk 2 to DNA . ^^^ Cyclin E proteins with mutated substrate selection ( Met Arg Ala Ile Leu ; MRAIL ) motifs fail to bind Cdc 6 , fail to compete with endogenous cyclin E Cdk 2 for chromatin binding , and fail to rescue replication in cyclin E depleted extracts . ^^^ Thus , the cyclin E Cdc 6 interaction that localizes the Cdk 2 complex to chromatin is important for DNA replication . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| It is recruited to chromatin by the origin recognition complex ( ORC ) , Cdc 6 , and Cdt 1 , and it is activated at the G ( 1 ) / S transition by Cdc 45 and the protein kinases Cdc 7 and Cdk 2 . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the replication initiation factors Cdt 1 and Cdc 6 along with cyclin A cdk 2 promotes rereplication in human cancer cells with inactive p 53 but not in cells with functional p 53 . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| Employing this protocol , analyses of chromatin bound MCM 2 , MCM 3 , Cdc 6 and cdk 2 suggests that the ' classical ' prereplication complex is already formed during hypoxia . . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| We further showed that Tat interacted with a number of cell cycle related proteins including cyclin A , cyclin B , cyclin D 3 , Cdk 2 , Cdk 4 , Cdk1 / Cdc2 , cdc 6 , p 27 , p 53 , p 63 , hdlg , and PCNA . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| Many genes whose expression is regulated by E2F family transcription factors , including CDK 2 , CCNE 1 , CDC 6 , CDC 2 , MCM 2 , were significantly down regulated at 24 hr post IR . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| RNA interference mediated depletion and chromatin immunoprecipitation assays show that endogenous ACTR directly controls the expression of genes important for initiation of DNA replication , which include cdc 6 , cdc25A , MCM 7 , cyclin E , and Cdk 2 . ^^^ |
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| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| Here , we use Xenopus egg extracts to show that the recruitment of cohesins to chromosomes requires fully licensed chromatin and is dependent on ORC , Cdc 6 , Cdt 1 and MCM 2 7 , but is independent of Cdk 2 . ^^^ |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| To confirm that the sequence surrounding serine 1237 was responsible for the differential regulation by Cdk 1 and Cdk 2 , we replaced 4 amino acids flanking the phosphorylation site to mimic a known Cdk 2 phosphorylation site present in the Cdc 6 protein . ^^^ |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| Cdc 6 and pRb , possible targets of Cdk 2 phosphorylation , behaved differentially . ^^^ |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P24941 |
Pubmed |
SVM Score :0.0 |
| NA |
|