| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.84515192 |
| CDC 6 interacts specifically with the active Cyclin A / CDK2 complex in vitro and in vivo , but not with Cyclin E or Cyclin B kinase complexes . 0.84515192^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Cyclin A dependent kinase activity affects chromatin binding of ORC , Cdc 6 , and MCM in egg extracts of Xenopus laevis . ^^^ High cyclin A dependent kinase activity inhibits the binding and promotes the release of Xenopus ORC , Cdc 6 , and MCM from sperm chromatin , but has no effect on chromatin binding of control proteins . ^^^ Cyclin A together with ORC , Cdc 6 and MCM proteins is bound to sperm chromatin in DNA replicating pseudonuclei . ^^^ We conclude that chromatin bound cyclin A kinase controls DNA replication by protein phosphorylation and chromatin release of Cdc 6 and MCM , whereas soluble cyclin E kinase prevents rereplication during the cell cycle by the inhibition of premature MCM chromatin association . . ^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| We have been able to identify a number of genes , including B myb , cyclin A , cdc 2 , cdc 6 , and DHFR , whose expression is dependent on the presence of E2F3 but not E2F1 . ^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| This G 1 arrest is associated with down regulation of a subset of E2F targets including cyclin A , E2F1 and CDC 6 . ^^^ |
|
| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| In S phase , phosphorylation of components of the DNA replication machinery such as CDC 6 by cyclin A CDK is believed to be important for initiation of DNA replication and to restrict the initiation to only once per cell cycle . ^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Cdc 6 chromatin affinity is unaffected by serine 54 phosphorylation , S phase progression , and overexpression of cyclin A . ^^^ Ectopically expressed Cdc 6 is translocated from the nucleus during S phase in a cyclin A Cdk 2 dependent process , suggesting that reinitiation of DNA replication is prevented by removal of phosphorylated Cdc 6 from chromatin after origin firing . ^^^ To resolve the questions regarding regulation of endogenous Cdc 6 , we cloned the cDNA encoding the Chinese hamster Cdc 6 homolog and specifically focused on analyzing the localizations and chromatin affinities of endogenous and exogenous proteins during S phase and following overexpression of cyclin A . ^^^ In agreement with other reports , ectopically expressed Cdc 6 translocates from the nucleus during S phase and in response to overexpressed cyclin A . ^^^ Overexpression of cyclin A is unable to alter the nuclear localization of Cdc 6 . ^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| We further showed that Tat interacted with a number of cell cycle related proteins including cyclin A , cyclin B , cyclin D 3 , Cdk 2 , Cdk 4 , Cdk1 / Cdc2 , cdc 6 , p 27 , p 53 , p 63 , hdlg , and PCNA . ^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| Of these eight genes , only Cdc 6 , cyclin A , and cyclin B were downregulated at both the mRNA and protein level in Casodex treated cells as compared to control cells . ^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| APC / C dependent Cdc 6 proteolysis prevents pre RC assembly in quiescent cells and , when cells reenter the cell cycle from quiescence , CDK dependent Cdc 6 stabilization allows Cdc 6 to accumulate before the licensing inhibitors geminin and cyclin A which are also APC / C substrates . ^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| On the protein level , Cdc 6 , ASK , cyclin A 2 , and cyclin B 1 are extremely abundant in ES cells compared with MEFs . ^^^ |
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| Interacting proteins: P20248 and Q99741 |
Pubmed |
SVM Score :0.0 |
| NA |
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