| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.55037348 |
| CDC 6 interacts specifically with the active Cyclin A / CDK2 complex in vitro and in vivo , but not with Cyclin E or Cyclin B kinase complexes . 0.55037348^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.53571831 |
| Cdc 6 synergizes with physiological levels of cyclin E / Cdk2 to induce semiconservative DNA replication in quiescent cells whereas cyclin A / Cdk2 is unable to collaborate with Cdc 6 . 0.53571831^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Genetic evidence suggested that the inhibition of mitotic cyclin dependent kinase activities was dependent on and possibly mediated through the CDC 6 gene product . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| We have compared the periodic fluctuation of mRNAs encoded by CDC 6 , a cell cycle gene controlling initiation of DNA replication , and CLN 1 , a G 1 cyclin gene expressed at late G 1 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| This element , which we call an ECB ( early cell cycle box ) , was first identified in the SWI 4 promoter , but it is also present in the promoter of a G 1 cyclin CLN 3 , as well as in the promoters of three DNA replication genes : CDC 6 , CDC 47 , and CDC 46 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| The transcription of a subset of these genes , which includes Cdc 6 , cyclin E , and cdk 2 , is also regulated during the cell cycle . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Our data also demonstrate that the human CDC 6 protein ( hCDC 6 ) is essential and limiting for DNA synthesis , since microinjection of an anti CDC 6 rabbit antiserum blocks DNA synthesis and CDC 6 cooperates with cyclin E to induce entry into S phase in cotransfection experiments . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cyclin A dependent kinase activity affects chromatin binding of ORC , Cdc 6 , and MCM in egg extracts of Xenopus laevis . ^^^ We find that chromatin binding of ORC , Cdc 6 , and MCM proteins does not require cyclin dependent kinase activities . ^^^ High cyclin A dependent kinase activity inhibits the binding and promotes the release of Xenopus ORC , Cdc 6 , and MCM from sperm chromatin , but has no effect on chromatin binding of control proteins . ^^^ Cyclin A together with ORC , Cdc 6 and MCM proteins is bound to sperm chromatin in DNA replicating pseudonuclei . ^^^ High cyclin E kinase activity allows the binding of Xenopus ORC and Cdc 6 , but not MCM , to sperm chromatin , even though the kinase does not phosphorylate MCM directly . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| We have been able to identify a number of genes , including B myb , cyclin A , cdc 2 , cdc 6 , and DHFR , whose expression is dependent on the presence of E2F3 but not E2F1 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Chromatin bound Cdc 6 persists in S and G 2 phases in human cells , while soluble Cdc 6 is destroyed in a cyclin A cdk 2 dependent process . ^^^ Recombinant cyclin A cdk 2 can completely substitute for the nucleus in promoting destruction of soluble Xenopus and human Cdc 6 . ^^^ We propose that cyclin A cdk 2 phosphorylation results in destruction of any Cdc 6 not assembled into replication complexes , but that assembled proteins remain , in the phosphorylated state , in the nucleus . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cyclin E uses Cdc 6 as a chromatin associated receptor required for DNA replication . ^^^ In the first phase , the origin recognition complex and Cdc 6 prereplication proteins , but not the minichromosome maintenance complex , are necessary and biochemically sufficient for ATP dependent binding of cyclin E Cdk 2 to DNA . ^^^ We find that cyclin E binds the NH ( 2 ) terminal region of Cdc 6 containing Cy Arg 10 Leu ( RXL ) motifs . ^^^ Cyclin E proteins with mutated substrate selection ( Met Arg Ala Ile Leu ; MRAIL ) motifs fail to bind Cdc 6 , fail to compete with endogenous cyclin E Cdk 2 for chromatin binding , and fail to rescue replication in cyclin E depleted extracts . ^^^ Cdc 6 proteins with mutations in the three consensus RXL motifs are quantitatively deficient for cyclin E binding and for rescuing replication in Cdc 6 depleted extracts . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| The cyclin N terminal helix mutations have a significant effect on the interaction between the cyclin CDK complex and specific substrates , Xenopus Cdc 6 and Cdc25C . ^^^ These results suggest that the N terminal helix of mitotic cyclins is required for specific interactions with CDKs and that to interact with CDK , specific substrates Cdc 6 and Cdc25C require the CDK to be associated with a cyclin . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cdc 6 cooperates with Sic 1 and Hct 1 to inactivate mitotic cyclin dependent kinases . ^^^ Deletion of the CDK interacting domain of Cdc 6 does not inhibit the function of origins of DNA replication during S phase , but instead causes a delay in mitotic exit ; this delay is accentuated in the absence of Sic 1 or of cyclin degradation . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| We show here that Xic 1 ubiquitination does not require phosphorylation by cyclin E Cdk 2 , but it does require nuclear accumulation of the Xic 1 cyclin E Cdk 2 complex and recruitment of this complex to chromatin by the origin recognition complex together with Cdc 6 replication preinitiation factors ; it also requires an activation step necessitating cyclin E Cdk 2 kinase and SCF ubiquitin ligase activity , and additional factors associated with mini chromosome maintenance proteins , including the inactivation of geminin . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Consensus E2F binding sites were identified in promoters of several cell cycle related genes , including the D type cyclin CycD 3 and the Arabidopsis homologue of the replication origin protein CDC 6 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cyclin E stimulates replication complex assembly by cooperating with Cdc 6 , to make G 1 nuclei competent to replicate in vitro . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| This G 1 arrest is associated with down regulation of a subset of E2F targets including cyclin A , E2F1 and CDC 6 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| In S phase , phosphorylation of components of the DNA replication machinery such as CDC 6 by cyclin A CDK is believed to be important for initiation of DNA replication and to restrict the initiation to only once per cell cycle . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Interestingly , cdc 6 protein expression was reestablished in K 562 cells that underwent endomitosis after transient or stable cyclin E overexpression . ^^^ The high levels of cyclin E reached in these cells appeared to influence the stabilization of cdc 6 protein rather than its RNA transcription rate . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the replication initiation factors Cdt 1 and Cdc 6 along with cyclin A cdk 2 promotes rereplication in human cancer cells with inactive p 53 but not in cells with functional p 53 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| We evaluated the hypothesis that the N terminal region of the replication control protein Cdc 6 acts as an inhibitor of cyclin dependent kinase ( Cdk ) activity , promoting mitotic exit . ^^^ During late mitosis , Cdc 6 is present at levels comparable with Sic 1 and binds specifically to the mitotic cyclin Clb 2 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cdc 6 is known to be phosphorylated by cyclin A cyclin dependent kinase 2 ( Cdk 2 ) , an event that promotes its exit from the nucleus and probably blocks it from initiating inappropriate DNA replication . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| For instance , DeltaNp73alpha increased the expression of EGR 1 and CDC 6 , whereas it decreased the mRNA levels of c MYC , cyclin A2 / CCNA2 , NF kappaB 1 , ODC 1 , and RET finger protein / RFP . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Cdc 6 chromatin affinity is unaffected by serine 54 phosphorylation , S phase progression , and overexpression of cyclin A . ^^^ Ectopically expressed Cdc 6 is translocated from the nucleus during S phase in a cyclin A Cdk 2 dependent process , suggesting that reinitiation of DNA replication is prevented by removal of phosphorylated Cdc 6 from chromatin after origin firing . ^^^ To resolve the questions regarding regulation of endogenous Cdc 6 , we cloned the cDNA encoding the Chinese hamster Cdc 6 homolog and specifically focused on analyzing the localizations and chromatin affinities of endogenous and exogenous proteins during S phase and following overexpression of cyclin A . ^^^ In agreement with other reports , ectopically expressed Cdc 6 translocates from the nucleus during S phase and in response to overexpressed cyclin A . ^^^ Overexpression of cyclin A is unable to alter the nuclear localization of Cdc 6 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Rb , as well as Cdc 6 , NPAT , and nucleophosmin , critical components of cell proliferation and DNA replication , respectively , are targets of Cyclin E / Cdk2 phosphorylation . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| The actions of origin recognition complex and CDC 6 are suppressed through phosphorylation by cyclin dependent kinases ( Cdks ) after S phase to prohibit rereplication . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| We further showed that Tat interacted with a number of cell cycle related proteins including cyclin A , cyclin B , cyclin D 3 , Cdk 2 , Cdk 4 , Cdk1 / Cdc2 , cdc 6 , p 27 , p 53 , p 63 , hdlg , and PCNA . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| CDK interaction with the NTD of Cdc 6 is mediated by the cyclin subunit Clb 2 , and could be reconstituted with recombinant Clb 2 protein and synthetic NTD peptides . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| On the protein level , Cdc 6 , ASK , cyclin A 2 , and cyclin B 1 are extremely abundant in ES cells compared with MEFs . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Under these conditions , proteins such as cyclin B 1 and Cdc 6 , which are normally detected only in meiosis 2 , were massively produced in meiosis 1 before the occurrence of the nuclear envelope breakdown . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Of these eight genes , only Cdc 6 , cyclin A , and cyclin B were downregulated at both the mRNA and protein level in Casodex treated cells as compared to control cells . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| While the deletion mutant retains the ability to mediate the stabilization of cyclin B 1 , cdc 6 , and geminin in infected cells , its capacity to upregulate the expression of cyclin E has been reduced . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Both cyclin A / Cdk2 and Cdk 1 efficiently phosphorylated the CDP / Cux ( Cdc 6 ) mutant and inhibited its DNA binding activity . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| APC / C dependent Cdc 6 proteolysis prevents pre RC assembly in quiescent cells and , when cells reenter the cell cycle from quiescence , CDK dependent Cdc 6 stabilization allows Cdc 6 to accumulate before the licensing inhibitors geminin and cyclin A which are also APC / C substrates . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| Our analysis suggests that among 47 cell cycle related genes common to the 2nd experiment ( thymidine thymidine method ) and the 4th experiment ( thymidine nocodazole method ) : ( 1 ) the amplitudes of CDC 6 and PCNA , which are expressed during G1 / S phase , are smaller in the 2nd experiment than in the 4th , while the amplitude of CDC 20 , which is expressed during G2 / M phase , is smaller in the 4th experiment ; and ( 2 ) the two cell arresting methods had little impact on the amplitudes of the other 43 genes in the 2nd and 4th experiments . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| As for the positive controls adozelesin and cisplatin , arrest was accompanied by phosphorylation of ATM kinase ; dephosphorylation of pRB ; decreases in RF C , cyclin D 1 , Cdc25A , and Cdc 6 ; and increases in p 21 . ^^^ Induction of phosphatase activity may represent one mechanism of RNS induced arrest , for the PP1 / PP2A phosphatase inhibitor okadaic acid inhibited dephosphorylation of pRB ; prevented decreases in the levels of RF C , cyclin D 1 , Cdc 6 , and Cdc25A ; and bypassed arrest by SIN 1 or NO ( 2 ) , but not cisplatin or adozelesin . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| RNA interference mediated depletion and chromatin immunoprecipitation assays show that endogenous ACTR directly controls the expression of genes important for initiation of DNA replication , which include cdc 6 , cdc25A , MCM 7 , cyclin E , and Cdk 2 . ^^^ |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99741 and P12004 |
Pubmed |
SVM Score :0.0 |
| NA |
|