| Interacting proteins: P48764 and Q99653 |
Pubmed |
SVM Score :0.0 |
| Ubiquitous calcineurin B homologous protein ( CHP or p 22 ) is co localized and co immunoprecipitated with expressed NHE 1 , NHE 2 , or NHE 3 independently of its myristoylation and Ca2+ binding , and its binding site was identified as the juxtamembrane region within the carboxyl terminal cytoplasmic domain of exchangers . ^^^ |
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| Interacting proteins: P48764 and Q99653 |
Pubmed |
SVM Score :0.0 |
| Acute regulation of Na / H exchanger NHE 3 by adenosine A ( 1 ) receptors is mediated by calcineurin homologous protein . ^^^ We tested the role of NHE 3 CHP interaction in mediating CPA induced inhibition of NHE 3 in opossum kidney ( OK ) and Xenopus laevis uroepithelial ( A 6 ) cells . ^^^ Both native and transfected NHE 3 and CHP are present in the same immuno complex by co immunoprecipitation . ^^^ CPA ( 10 ( 6 ) M ) increases CHP NHE 3 interaction by 30 60 % ( native and transfected proteins ) . ^^^ Direct CHP NHE 3 interaction is evident by yeast two hybrid assay ( bait , NHE 3 ( C terminus ) ; prey , CHP ) ; the minimal interacting region is localized to the juxtamembrane region of NHE 3 ( C terminus ) ( amino acids 462 552 of opossum NHE 3 ) . ^^^ |
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