| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.6456584 |
| The N terminal activation domain of Cdx 2 is required for maximal synergy with HNF1alpha . 0.6456584^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :1.3269501 |
| In addition , Cdx 2 was shown to cooperate with HNF1alpha to synergistically activate the UGT1A8 , 1A9 , and 1A10 promoters . 1.3269501^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.72698814 |
| In addition , Cdx 2 was shown to cooperatively activate the UGT2B7 promoter in conjunction with hepatocyte nuclear factor 1alpha ( HNF1alpha ) , a mechanism previously observed to regulate other intestine specific genes . 0.72698814^^^ |
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| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| HNF 1alpha augmented the Cdx 2 induced but not Cdx 1 induced transcriptional activation of the human claudin 2 promoter . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Cases were stained with antibodies against Cdx 2 , thyroid transcription factor 1 ( TTF 1 ) , cytokeratin ( CK ) 7 , and CK 20 . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Cdx 2 regulates the UGT1A8 and 1A10 promoters in cooperation with hepatocyte nuclear factor 1alpha ( HNF1alpha ) . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Herein , we show that hepatocyte nuclear factor 1 alpha ( HNF 1 alpha ) , GATA 4 , and caudal related homeodomain proteins Cdx 2 and Cdx 1 are the primary transcription factors from the adult mouse intestinal epithelium to interact with the SIF 3 , GATA , and SIF 1 elements of the SI promoter . ^^^ We wanted to study whether HNF 1 alpha , GATA 4 , and Cdx 2 can cooperate in the regulation of SI gene expression . ^^^ HNF 1 alpha , GATA 4 , and Cdx 2 interact in vitro and in vivo . ^^^ These factors activate SI promoter activity in cotransfection experiments where GATA 4 requires the presence of both HNF 1 alpha and Cdx 2 . ^^^ These findings imply a combinatory role of HNF 1 alpha , Cdx 2 , and GATA 4 for the time and position dependent regulation of SI transcription during development . . ^^^ |
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| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Transcriptional regulation of the beta3Gal T 5 gene was consistent with that of members of the Cdx and HNF 1 families in two in vivo systems . 1 ) During in vitro differentiation of Caco 2 cells , transcriptional up regulation of beta3Gal T 5 was observed in correlation with the increase in transcripts for Cdx 2 and HNF1alpha . 2 ) Both transcript and protein levels of beta3Gal T 5 were determined to be significantly reduced in colon cancer . ^^^ Four factors , Cdx 1 , Cdx 2 , HNF1alpha , and HNF1beta , could bind to GCE and transcriptionally activate the beta3Gal T 5 gene . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Control of differentiation induced calbindin D9k gene expression in Caco 2 cells by cdx 2 and HNF 1alpha . ^^^ Whereas a 60 % reduction of CaBP mRNA in the duodenum of HNF 1alpha null mice confirmed the physiological importance of HNF 1alpha for CaBP gene regulation , additional studies showed that maximal CaBP expression requires the presence of both HNF 1alpha and cdx 2 . ^^^ Our data suggest that cdx 2 is a permissive factor that influences basal CaBP expression in enterocytes and that HNF 1alpha modulates CaBP gene expression during cellular differentiation . . ^^^ |
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| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| The changes of connexin 32 and claudin 2 may be controlled at the transcriptional level via NF kappaB , HNF 1alpha , and CDX 2 . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| We thus investigated the expression of claudin 2 in relation to the transcription factors CDX 2 , HNF 1alpha , and GATA 4 in the human intestine . ^^^ The functional role of CDX 2 , HNF 1alpha , and GATA 4 on claudin 2 regulation was also examined by ectopic expression studies in intestinal cell models . ^^^ CDX 2 and HNF 1alpha were expressed along the entire intestine whereas GATA 4 was undetectable in the colon . ^^^ Like in the colonic epithelium , GATA 4 was found to be also lacking in Caco 2 cells while CDX 2 and HNF 1alpha were present at significant levels . ^^^ Cotransfection experiments showed that the claudin 2 promoter was activated by CDX 2 , HNF 1alpha , and GATA 4 in a cooperative manner . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| While a resemblance to intestinal adenocarcinoma by light microscopy is present , immunohistochemical studies comparing these carcinomas with metastatic colorectal adenocarcinoma clearly show a respiratory phenotype with the neoplastic cells expressing thyroid transcription factor 1 and cytokeratin 7 to the exclusion of cytokeratin 20 , and failing to express CDX 2 . ^^^ |
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| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Intestine specific gene expression is regulated by transcription factors that are becoming well characterized , including CDX 1 , CDX 2 and HNF 1 . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Transcription factors activating the expression of genes in the intestine include CDX 1 , CDX 2 and HNF1alpha and their actions are increasingly well characterized . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| We studied the usefulness of differential expression of CDX 2 and thyroid transcription factor 1 ( TTF 1 ) in 78 gastrointestinal and pulmonary carcinoids and their metastases ( n = 10 ) . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Data analysis led to a simplified diagnostic panel and decision tree containing 10 markers only : CA 125 , CDX 2 , cytokeratins 7 and 20 , estrogen receptor , gross cystic disease fluid protein 15 , lysozyme , mesothelin , prostate specific antigen , and thyroid transcription factor 1 . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Expression of PDX 1 , a repressor of sucrase and lactase promoters , was 4 . 5 fold higher in iron deficiency , whereas CDX 2 , GATA 4 , and HNF 1 levels were not significantly different . ^^^ |
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| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| To investigate the molecular mechanism ( s ) responsible for the tissue specific expression of ACAT 2 , we identified five cis elements within the human ACAT 2 promoter , four for the intestinal specific transcription factor CDX 2 ( caudal type homeobox transcription factor 2 ) , and one for the transcription factor HNF1alpha ( hepatocyte nuclear factor 1alpha ) . ^^^ Results of luciferase reporter and electrophoretic mobility shift assays show that CDX 2 and HNF1alpha exert a synergistic effect , enhancing the ACAT 2 promoter activity through binding to these cis elements . ^^^ In undifferentiated Caco 2 cells , the ACAT 2 expression is increased when exogenous CDX 2 and / or HNF1alpha are expressed by co transfection . ^^^ In differentiated Caco 2 cells , the ACAT 2 expression significantly decreases when the endogenous CDX 2 or HNF1alpha expression is suppressed by using RNAi ( RNA interference ) technology . ^^^ The expression levels of CDX 2 , HNF1alpha , and ACAT 2 are all greatly increased when the Caco 2 cells differentiate to become intestinal like cells . ^^^ |
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| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Furthermore , CDX 1 , CDX 2 , C / EBPalpha , and C / EBPbeta bound to HNF 1alpha and HNF 1beta . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| Distinct spatial expressions patterns were detected along the length of the small intestine for PDX 1 , Cdx 2 , GATA 4 , GATA 5 , GATA 6 , HNF 1alpha , HNF 1beta and CDP transcription factor genes . ^^^ |
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| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| The effect of cytostatic drug treatment on intestine specific transcription factors Cdx 2 , GATA 4 and HNF 1alpha in mice . ^^^ The objective of this study was to investigate the gene expression of the small intestinal transcription factors HNF 1alpha , Cdx 2 , GATA 4 in an experimental model of methotrexate ( MTX ) induced intestinal damage , and to correlate these alterations with histological damage , epithelial proliferation and differentiation . ^^^ HNF 1alpha , Cdx 2 and GATA 4 are critical transcription factors in epithelial differentiation , and in combination they act as promoting factors of the sucrase isomaltase ( SI ) gene , an enterocyte specific differentiation marker which is distinctly downregulated after MTX treatment . ^^^ Intestinal damage was most severe at day 3 and was associated with decreased expression of the transcriptional factors HNF 1alpha , Cdx 2 and GATA 4 , which correlated well with decreased expression of SI , and seemed inversely correlated with enhanced proliferation of epithelial crypt cells . ^^^ Since HNF 1alpha , Cdx 2 and GATA 4 are critical for intestine specific gene expression and therefore crucial in epithelial differentiation , these results may explain , at least in part , why intestinal differentiation is compromised during MTX treatment . . ^^^ |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q99626 and P20823 |
Pubmed |
SVM Score :0.0 |
| NA |
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