| A RING finger protein Praja 1 regulates Dlx 5 dependent transcription through its ubiquitin ligase activity for the Dlx / Msx interacting MAGE / Necdin family protein , Dlxin 1 . ^^^ In an attempt to elucidate the molecular function of Dlxin 1 , two closely related RING finger proteins , Praja 1 and Neurodap 1 , were isolated by yeast two hybrid screening using the C terminal necdin homology domain of Dlxin 1 as bait . ^^^ Glutathione S transferase pull down and immunoprecipitation / Western blotting assays following co transfection of Dlxin 1 and Praja 1 revealed that Praja 1 binds to the C terminal necdin homology domain of Dlxin 1 in vitro and in vivo , respectively . ^^^ |