Pubmed abstracts for Protein-Protein Interaction search result :


Interacting proteins: Q99523 and P08138 Pubmed SVM Score :1.010419
Together with the recent finding that p75NTR interacts with the transmembrane protein sortilin to form a different receptor complex with cell death promoting activity , the results of Mi et al . indicate that p75NTR exerts its diverse cellular functions by associating with function specific co receptors . . 1.010419^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
B lymphocytes express the BDNF receptors p75NTR and TrkB ( gp 95 ) , while no BDNF expression was found . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) and neurotrophin 3 ( NT 3 ) are members of a family of trophic factors designated the neurotrophins , each of which can bind to the low affinity NGF receptor ( LNGFR ) . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Like the structurally related proteins nerve growth factor ( NGF ) and brain derived neurotrophic factor ( BDNF ) , NT 3 also binds to the low affinity NGF receptor , a molecule that we suggest to designate low affinity neurotrophin receptor ( LANR ) . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
These mitogenic effects are absolutely dependent on expression of gp140trk receptors , but do not require the presence of the previously described low affinity NGF receptor . gp140trk also serves as a receptor for the related factor neurotrophin 3 ( NT 3 ) , but not for brain derived neurotrophic factor . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
TrkB mediates BDNF / NT 3 dependent survival and proliferation in fibroblasts lacking the low affinity NGF receptor . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
To obtain insight into the site and stage specificity of nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , and neurotrophin 3 ( NT 3 ) action in vivo , we compared the expression patterns of the genes for these three related neurotrophic factors as well as for the NGF receptor in developing and adult rats . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Here we demonstrate that all three of these neuronal survival molecules bind similarly to the low affinity NGF receptor , but that BDNF and NT 3 , unlike NGF , do not act via the high affinity NGF receptor . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Like NGF , BDNF and NT 3 crosslinked to the low affinity NGF receptor ( p75NGFR ) on PC 12 cells . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
In addition to gp145trkB , the pyramidal neuronal cultures expressed transcripts specific for the NT 3 receptor gp145trkC , but not for the high affinity NGF receptor gp140trk or for p75LNGFR , a low affinity receptor for all known members of the NGF family of neurotrophins including the gp145trkB ligands , BDNF and NT 4 . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
In rat or cat , the topography of 125I NT 3 binding differed from that reported for 125I NGF binding or for the low affinity NGF receptor . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
At elevated concentrations , beyond the saturation of its high affinity receptors , NT 3 supports the survival of some E 11 sympathetic neurons , an effect that might be mediated by the high affinity NGF receptor . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , and neurotrophin 3 ( NT 3 ) selectively bind to distinct members of the Trk family of tyrosine kinase receptors , but all three bind with similar affinities to the neurotrophin receptor p 75 ( p75NTR ) . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
When 70W cells were exposed to antisense oligonucleotides directed against p75NTR mRNA , there was a reduction in NGF and NT 3 binding , and the neurotrophins failed to enhance Matrigel invasion . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Nerve growth factor ( NGF ) , brain derived neurotrophic factor , neurotrophin 3 ( NT 3 ) , and NT 5 stimulated sphinomyelin hydrolysis with similar kinetics in p75NTR NIH 3T3 cells . ^^^ Although brain derived neurotrophic factor ( 10 ng / ml ) was slightly more potent than NGF at inducing sphingomyelin hydrolysis , NT 3 and NT 5 induced significant hydrolysis ( 30 35 % ) at 0 . 1 to 1 ng / ml in p75NTR NIH 3T3 cells . ^^^ NT 3 did not induce sphingomyelin hydrolysis in Trk C NIH 3T3 cells nor in cells expressing a mutated p75NTR containing a 57 amino acid cytoplasmic deletion , thus demonstrating the role of p75NTR in this signal transduction pathway . ^^^ Interestingly , NT 3 ( 10 ng / ml ) induced the same extent of sphingomyelin hydrolysis in PC 12 cells as was apparent in p75NTR NIH 3T3 cells . ^^^ However , in the presence of NGF , NT 3 was unable to induce sphingomyelin hydrolysis , raising the possibility that Trk was modulating p75NTR dependent sphingomyelin hydrolysis . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
The in vitro survival of TH neurons devoid of p 75 NGFR is supported by NT 3 , implying that NT 3 may be trophic for a subset of tuberoinfundibular DA neurons . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
PNT 1 was shown to efficiently block the chemical crosslinking of NGF , BDNF , and NT 3 to their cognate Trk receptors and to the low affintiy NGF receptor expressed on neuronal and nonneuronal cells . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Function perturbing antibodies to the p 75 low affinity NGF receptor potentiated the NT 3 responses of both forms of TrkA in the transfected PC12nnr5 cell lines , suggesting that the low affinity NGF receptor suppresses the ability of TrkA to respond to NT 3 . . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
TrkC , the high affinity NT 3 receptor , and trkA , the high affinity NGF receptor , are both expressed from the early OLP through the mature OL stage . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
This is the first demonstration that K 252b potentiates a trkA mediated biological nonneuronal response by NT 3 that occurs independent of p75NTR and appears to be both ligand and receptor specific . . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
For nerve growth factor ( NGF ) , these are p75NTR ( p 75 ) and p140trk ( referred to as trk or trkA ) , which binds both BDNF and neurotrophin ( NT ) 4 / 5 , and trkC receptor , which binds only NT 3 . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
When 70W cells were exposed to antisense oligonucleotides directed against p75NTR mRNA , there was a reduction in NGF and NT 3 binding , and the neurotrophins failed to enhance Matrigel invasion . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
When 70W cells were exposed to anti sense oligonucleotides directed against p75NTR mRNA , there was a reduction in NGF and NT 3 binding , and the neurotrophins failed to enhance Matrigel invasion . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
In contrast , human bone marrow cells consistently expressed mRNA for trkB ( the BDNF and NT 4 receptor ) and displayed variable expression of mRNA coding for trkA ( the tyrosine kinase NGF receptor ) and LNGFR ( the low affinity NGF receptor ) , whereas mRNA for trkC ( the NT 3 receptor ) was not expressed . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
The situation is complex , however , in that NT 3 ( / ) mutant mice show far greater neuron loss than mice deficient in the NT 3 receptor TrkC , suggesting that NT 3 may support many DRG neurons via actions on the NGF receptor TrkA . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Here we demonstrate that NGF but not neurotrophin 3 ( NT 3 ) or brain derived neurotrophic factor ( BDNF ) induced apoptosis in p75NTR expressing human neuroblastoma SK N MC cells . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
The suppressive or inhibitory influence of NT 3 and RA is the result of NT 3 binding to the low affinity receptor , p75NTR , not the result of RA increasing mRNA levels for the high affinity receptor , trkC . ^^^ However , trk may act with p75NTR , as disruption of trk signalling alleviates the inhibitory response induced by NT 3 and RA . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
NGF antagonizes brain derived neurotrophic factor ( BDNF ) and neurotrophin 3 ( NT 3 ) mediated survival in control but not p75NTR deficient motoneurons . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
To determine the role of the p 75 neurotrophin receptor ( p75NTR ) in sympathetic neuron development , we crossed transgenic mice with mutations in p75NTR , nerve growth factor ( NGF ) and neurotrophin 3 ( NT 3 ) . ^^^ When NT 3 levels are reduced ( p75NTR / NGF+ / NT 3 + / ) , neuron number decreases compared to p75NTR / NGF+ / NT3+ / + . ^^^ Thus , without p75NTR , NT 3 substitutes for NGF , suggesting that p 75 alters the neurotrophin specificity of TrkA in vivo . . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Binding of four mammalian neurotrophins , nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , and neurotrophin 4 / 5 ( NT 4 / 5 ) , to p75NTR is studied by molecular modeling based on 10 ray structures of the neurotrophins and the extracellular domain of p55TNFR , a homologue of p75NTR . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Expression of wild type p75NTR created a state of neurotrophin dependence : cells could be rescued by nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , or neurotrophin 3 ( NT 3 ) , but not by a mutant NGF that binds well to Trk A but poorly to p75NTR . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Furthermore , p75NTR / NADE induced cell death was dependent on NGF but not BDNF , NT 3 , or NT 4 / 5 , and the recruitment of NADE to p75NTR ( intracellular domain ) was dose dependent . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
In the present study , we assessed the temporal change in gene expression of neurotrophins , NGF , BDNF , and NT 3 , and their receptors , low affinity neurotrophin receptor ( p75NTR ) and Trks A , B , and C , by RT PCR technique in the liver of rats treated with lead nitrate ( LN ; 0 . 1 mmol / kg body weight ) , an inducer of liver hyperplasia . ^^^ The mRNAs for NGF , BDNF with exon 4 , NT 3 , p75NTR , and all Trk members were detected in the LN untreated liver . ^^^ LN treatment resulted in increases in the levels of NGF , BDNF with exon 4 , NT 3 , p75NTR , and TrkA mRNAs and further led to expression of BDNF mRNA with exon 3 . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Although NGF , brain derived neurotrophic factor ( BDNF ) , and neurotrophin 3 ( NT 3 ) accelerated catagen in the organ cultured skin of C57BL / 6 mice , these neurotrophins failed to promote catagen development in the organ cultured p75NTR null skin . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
In the present study , the changes in expression of the neurotrophins ( NGF , BDNF , and NT 3 ) and their cognate receptors ( i . e . , trk and p75NTR ) during the progression of normal prostatic epithelial cells to malignancy were documented . ^^^ In contrast , malignant prostate epithelial cells directly secrete a series of neurotrophins ( i . e . , NGF , BDNF , and / or NT 3 ) and express at least one if not more of the trk receptor proteins ( i . e . , trk A , B , and / or C ) , while no longer expressing the p75NTR receptors . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
METHODS : Immunocytochemistry and Western blot analyses were performed using primary antibodies raised against NTs ( nerve growth factor [ NGF ] , brain derived neurotrophic factor [ BDNF ] , NT 3 , and NT 4 / 5 ) and NT receptors ( TrkA , TrkB , TrkC , and p75NTR ) . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Sections of pancreatic cancer and normal pancreatic tissue were examined by immunohistochemistry for the expression of the neurotrophins NGF , BDNF , NT 3 , NT 4 , and their receptors TrkA , TrkB , and TrkC , as well as the low affinity receptor , p75NTR . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Conditional mutant brains produced undetectable amounts of NT 3 mRNA , whereas the expression of the neurotrophin , BDNF , the NT 3 receptor , TrkC , and the nonselective , low affinity neurotrophin receptor p75NTR , were comparable to wild type . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Our data indicate that at embryonic day 16 . 5 ( E 16 . 5 ) , NT 3 and its receptors , p 75 neurotrophin receptor ( p75NTR ) and tyrosine kinase receptor , TrkC are not expressed at detectable levels in Merkel cells . ^^^ After a perinatal switch , however , Merkel cells in whiskers of newborn mice are immunoreactive for p75NTR , TrkC and NT 3 . ^^^ We draw three major conclusions from these observations : ( 1 ) NT 3 signaling through p75NTR or TrkC is not required for the development and prenatal survival of either a major subset or of all Merkel cells , ( 2 ) the postnatal survival of Merkel cells is supported by autocrine or paracrine NT 3 , rather than by neuron derived NT 3 , and ( 3 ) Merkel cell derived NT 3 is not a chemoattractant for innervating A beta fibers , but is likely to be involved in maintaining Merkel cell innervation postnatally . . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
For example , murine hair follicles ( HF ) show developmentally and spatio temporally stringently controlled expression of NTs , including nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , and NT 4 , and their cognate receptors , tyrosine kinase A C ( TrkA C ) and p 75 neurotrophin receptor ( p75NTR ) . ^^^ NT 3 / TrkC and NGF / TrkA signaling stimulate HF development , while NT 3 , NT 4 and BDNF inhibit the growth ( anagen ) of mature HF by the induction of apoptosis driven HF regression ( catagen ) . p75NTR stimulation inhibits HF development and stimulates catagen . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
We show here that their respective receptors , TrkA , TrkB , TrkC , as well as p 75 ( NTR ) ( the low affinity NGF receptor ) , are expressed in vitro by the nasal ensheathing cells ; the three neurotrophins promote purification and proliferation of these glial cells , with an optimal concentration of 50 ng / ml ; and human ensheathing cells can be easily biopsied and highly purified using a serum free medium supplemented with NT 3 . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
We directly compared TrkA and p75NTR for their ability to internalize NGF , and we also examined the endocytosis of iodinated brain derived neurotrophic factor ( BDNF ) and neurotrophin 3 ( NT 3 ) by p75NTR . ^^^ Surprisingly , cells expressing only p75NTR internalized far more BDNF or NT 3 than NGF . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
We have used immunofluorescence techniques with a panel of antibodies against known neurotrophin receptors ( trk A , trk B , trk C , p75NTR ) to map the locations of these receptors in the developing neuromuscular system of mice from our neurotrophin 3 ( NT 3 ) knockout colony . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
We show that p75NTR is expressed at highest levels in the region of the cerebellum where foliation is altered in BDNF and NT 3 mutants . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
At embryonic day 16 . 5 ( E 16 . 5 ) , neither NT 3 nor its primary receptors , TrkC and p75NTR are expressed by Merkel cells in the murine whisker . ^^^ At the time of birth , however , Merkel cells are immunoreactive for NT 3 , TrkC and p75NTR . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Here we report that proNGF creates a signalling complex by simultaneously binding to p75NTR and sortilin . ^^^ Thus sortilin acts as a co receptor and molecular switch governing the p75NTR mediated pro apoptotic signal induced by proNGF . . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
In embryonic day ( E ) 15 rat trigeminal ganglion ( TG ) , NGF receptor TrkA is expressed by small diameter neurons , NT 3 receptor TrkC and BDNF receptor TrkB are expressed by large diameter neurons . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Pro inflammatory cytokines upregulate the skin immunoreactivity for NGF , NT 3 , NT 4 and their receptor , p75NTR in vivo : a preliminary report . ^^^ As a first step toward exploring this , we studied in murine back skin ( C57BL / 6 ) whether intradermally injected interleukin 1beta ( IL 1beta ) , tumor necrosis factor alpha ( TNF alpha ) , and interferon gamma ( IFN gamma ) altered the cutaneous immunoreactivity patterns of nerve growth factor ( NGF ) , brain derived neurotrophic factor ( BDNF ) , neurotrophin 3 ( NT 3 ) , neurotrophin 4 ( NT 4 ) , Trk A , Trk B , Trk C and p75NTR and their receptors ( TrkA , TrkB , TrkC , p75NTR ) on the protein level in situ . ^^^ The cytokine cocktail upregulated NT 3 and NT 4 IR in the epidermis , increased NT 4 IR in selected cells of the proximal outer root sheath , and also enhanced IR of p75NTR , in the follicular dermal papilla . ^^^ Therefore , this pilot study provides the first preliminary indications that proinflammatory cytokines upregulate the cutaneous immunoreactivity of NGF , NT 3 , NT 4 and their receptor p75NTR in vivo . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
The activation of TrkC or TrkC / p75NTR by NT 3 affected heparanase activity and cell invasive properties of medulloblastoma cells in vitro . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
In the present study , we have investigated the expression profile of neurotrophins NGF , BDNF and NT 3 and their high affinity ( TrkA , TrkB , TrkC ) and common low affinity ( p75NTR ) receptors before and during neural differentiation of rat BMSCs by RT PCR . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
ProBDNF induces neuronal apoptosis via activation of a receptor complex of p75NTR and sortilin . ^^^ Because recent studies suggest that proNGF , the precursor form of NGF , is more active than mature NGF in inducing apoptosis after binding to p75NTR and a coreceptor , sortilin , we asked whether the precursor of BDNF ( proBDNF ) is also a proapoptotic ligand in the nervous system . proBDNF is secreted by cultured neurons , and recombinant proBDNF binds to sortilin . ^^^ In sympathetic neurons coexpressing sortilin and p75NTR , we found that proBDNF is an apoptotic ligand that induces death at subnanomolar concentrations . ^^^ In contrast , mature BDNF , but not proBDNF , is effective in inducing TrkB phosphorylation . proBDNF effects are dependent on cellular coexpression of both p75NTR and sortilin , because neurons deficient in p75NTR are resistant to proBDNF induced apoptosis , and competitive antagonists of sortilin block sympathetic neuron death . ^^^ Moreover , addition of preformed complexes of soluble sortilin and proBDNF failed to induce apoptosis of cells coexpressing both sortilin and p75NTR , suggesting that interaction of proBDNF with both receptors on the cell surface is required to initiate cell death . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Factors limiting the therapeutic application of neurotrophin proteins include their poor pharmacological properties and their pleiotropic effects mediated by interaction with Trk , p75NTR and sortilin receptors . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
We have investigated the effect of a non mutated 32 kDa human recombinant proNGF ( rhproNGF ) on cultured pig oligodendrocytes which express TrkA , p75NTR and sortilin . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
Mechanisms of proneurotrophin induced death were analyzed in cultured BF neurons , and required both p75NTR and its coreceptor sortilin . ^^^
Interacting proteins: Q99523 and P08138 Pubmed SVM Score :0.0
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