| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| A novel repressor , par 4 , modulates transcription and growth suppression functions of the Wilms ' tumor suppressor WT 1 . ^^^ In this study , we identified par 4 ( for prostate apoptosis response ) as a WT 1 interacting protein that itself functions as a transcriptional repressor . par 4 contains a putative leucine zipper domain and is specifically upregulated during apoptosis of prostate cells ( S . ^^^ The leucine repeat domain of par 4 was shown to interact with the zinc finger DNA binding domain of WT 1 . ^^^ Immunoprecipitation Western blot ( immunoblot ) analyses demonstrated in vivo WT 1 par 4 interactions . par 4 was ubiquitously expressed , and the protein was found in both the nucleus and the cytoplasm . ^^^ Functionally , par 4 inhibited transcription activated by WT 1 , but not by the related protein EGR 1 . ^^^ |
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| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| It appears that physical interactions of other cellular proteins ( p 53 and par 4 ) with WT 1 can modulate the function of WT 1 . ^^^ |
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| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| Besides ATF 4 and others we found Par 4 , a modulator of transcription factor WT 1 and mediator of apoptosis . ^^^ |
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| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| Par 4 is a coactivator for a splice isoform specific transcriptional activation domain in WT 1 . ^^^ A mutation in WT 1 found in Wilms ' tumor disturbs the interaction with par 4 and disrupts the function of the activation domain . ^^^ Analysis of WT 1 derivatives in cells treated to induce par 4 expression showed a strong correlation between the transcription function of the WT 1 17 amino acid insertion and the ability of WT 1 to regulate cell survival and proliferation . ^^^ |
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| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| Par 4 transcriptionally regulates Bcl 2 through a WT 1 binding site on the bcl 2 promoter . ^^^ We investigated the role of the product of the prostate apoptosis response gene 4 ( Par 4 ) and the product of the Wilms ' tumor 1 gene ( WT 1 ) in the regulation of Bcl 2 expression in prostate cancer cell lines . ^^^ The decrease in the Bcl 2 protein and transcript following all trans retinoic acid treatment was accompanied by changes in localization of Par 4 and an induction in the expression of WT 1 protein . ^^^ The involvement of WT 1 in the Par 4 mediated down modulation of Bcl 2 was further defined by blocking endogenous WT 1 expression , which resulted in an increase in Bcl 2 expression . ^^^ Finally , we detected Par 4 and WT 1 proteins binding to a previously identified WT 1 binding site on the bcl 2 promoter both in vitro and in vivo leading to a decrease in transcription from the bcl 2 promoter . ^^^ |
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| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| The WT 1 transcription factor modulates expression and activity of several prostate growth control genes ( i . e . par 4 , bcl 2 and AR ) in vitro . ^^^ After several months the indolent WT 1 LNCaP cells became proliferative forming small tumors lacking par 4 protein . ^^^ |
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| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| We have recently shown that the Par 4 protein binds , via its leucine zipper domain , to the zinc finger domain of Wilms ' tumor protein WT 1 ( R . ^^^ Finally , ectopic expression of WT 1 attenuated apoptosis , and coexpression of Par 4 but not a leucine zipperless mutant of Par 4 rescued the cells from the antiapoptotic effect of WT 1 . ^^^ |
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| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| We also find that the integrity of a domain within the WT 1 zinc fingers , responsible for mediating interaction between WT 1 and the transcriptional repressor par 4 , is disrupted in the EWS / WT1 fusion product . ^^^ |
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| Interacting proteins: Q96IZ0 and P19544 |
Pubmed |
SVM Score :0.0 |
| We show that two WT 1 interacting proteins , the product of a Denys Drash syndrome allele of wt 1 and prostate apoptosis response 4 protein , inhibit WT 1 mediated transactivation of Dax 1 . ^^^ |
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