| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.67257651 |
| Complex formation between Dlk and Par 4 was confirmed by GST pull down experiments and kinase reactions in vitro and coexpression experiments in vivo . 0.67257651^^^ |
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| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.0 |
| However , induction of apoptosis by Dlk requires its relocation to the cytoplasm , particularly association with the actin cytoskeleton , which is achieved through interaction with pro apoptotic protein Par 4 . ^^^ |
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| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.0 |
| Binding of Par 4 to the actin cytoskeleton is essential for Par 4 / Dlk mediated apoptosis . ^^^ Cell death mediated by Par 4 and its interaction partner DAP like kinase ( Dlk ) is characterized by dramatic changes of the cytoskeleton . ^^^ To uncover the role of the cytoskeleton in Par 4 / Dlk mediated apoptosis , we analyzed Par 4 for a direct association with cytoskeletal structures . ^^^ In rat fibroblasts , this microfilament association is essential for the pro apoptotic function of Par 4 , since both disruption of the actin cytoskeleton by cytochalasin D treatment and overexpression of Par 4 constructs impaired in actin binding result in a significant decrease of apoptosis induction by Par 4 and Dlk . ^^^ We propose a model , in which Par 4 recruits Dlk to stress fibers , leading to enhanced phosphorylation of the regulatory light chain of myosin 2 ( MLC ) and to the induction of apoptosis . . ^^^ |
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| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.0 |
| The latter results in enhanced phosphorylation of the regulatory light chain of myosin 2 ( MLC ) as has previously been shown for Par 4 mediated recruitment of DAP like kinase ( Dlk ) , suggesting that the recruitment of nuclear proteins involved in the regulation of apoptotic processes to the actin filament system by Par 4 represents a potent mechanism how Par 4 can trigger apoptosis . . ^^^ |
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| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.0 |
| Although enforced apoptosis caused by ectopic Daxx expression is caspase dependent in both cases , major differences between Fas / TRAIL induced apoptosis and doxorubicin induced apoptosis are observed in expression patterns of 10 linked inhibitor of apoptosis ( XIAP ) , p 53 , Bid , ZIP kinase , and prostate apoptosis response gene 4 ( Par 4 ) . ^^^ |
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| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.0 |
| Par 4 contains a leucine zipper domain ( Leu . zip ) that presumably mediates protein protein interactions critical for its functions in apoptosis . ^^^ Par 4 activity can be effectively blocked by overexpression of Leu . zip because it exerts a dominant negative action possibly by competitively blocking the interaction of Par 4 with other proteins . ^^^ Non apoptotic reduction in ChAT activity induced by Par 4 can be completely blocked by co overexpression of Leu . zip , indicating that enhanced Par 4 activity is a necessary event for cholinergic hypoactivity in PC 12 cells . ^^^ |
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| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.0 |
| ZIPK also binds and phosphorylates proapoptotic protein Par 4 . ^^^ Association of ZIPK with Daxx was enhanced by coexpression of Par 4 . ^^^ Conversely , small interfering RNA mediated reduction of ZIPK , Daxx , or Par 4 expression decreased activation of caspase and apoptosis induced by As ( 2 ) O ( 3 ) and IFN gamma . ^^^ These results suggest that ZIPK , in collaboration with Daxx and Par 4 , mediates a novel nuclear pathway for apoptosis . . ^^^ |
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| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43293 and Q96IZ0 |
Pubmed |
SVM Score :0.0 |
| NA |
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