| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.71305377 |
| Second , Spc97p interacts with Spc98p and Tub4p in the two hybrid system . 0.71305377^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| GCP 2 and GCP 3 colocalize with gamma tubulin at the centrosome , cosediment with gamma tubulin in sucrose gradients , and coimmunoprecipitate with gamma tubulin , indicating that they are part of the gamma tubulin complex . ^^^ The conservation of a complex involving gamma tubulin , GCP 2 , and GCP 3 from yeast to mammals suggests that structurally diverse microtubule organizing centers such as the yeast spindle pole body and the animal centrosome share a common molecular mechanism for microtubule nucleation . . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| Stoichiometry experiments revealed that there is a single copy of GCP 5 and multiple copies of gamma tubulin , GCP 2 , GCP 3 , and GCP 4 within the gamma tubulin complex . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| The amino terminal region of either CG NAP or kendrin indirectly associated with gamma tubulin through binding with gamma tubulin complex protein 2 ( GCP 2 ) and / or GCP 3 . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| The role of the centrosomes in microtubule nucleation remains largely unknown at the molecular level . gamma Tubulin and the two associated proteins h103p ( hGCP 2 ) and h104p ( hGCP 3 ) are essential . ^^^ Surprisingly , h76p shares partial sequence identity with human centrosomal proteins h103p and h104p , suggesting a common protein core . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| Fission yeast Alp 4 and Alp 6 are homologues of two conserved gamma TuC proteins , hGCP 2 and hGCP 3 , respectively . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| Spc98p and Spc97p of the yeast gamma tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p . ^^^ Previously , we have shown that the yeast gamma tubulin , Tub4p , forms a 6S complex with the spindle pole body components Spc98p and Spc97p . ^^^ It contained one molecule of Spc98p and Spc97p , and two or more molecules of Tub4p , but no other protein . ^^^ Genetic and biochemical data indicate that Spc98p and Spc97p of the Tub4p complex bind to the N terminal domain of the SPB component Spc110p . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| Overexpression of SPC 98 suppresses the temperature sensitivity conferred by mutations in region 1 but not the phenotypes conferred by mutations in the other two regions , suggesting that the amino terminus of Spc110p is involved in an interaction with the gamma tubulin complex composed of Spc97p , Spc98p , and Tub4p . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| Microtubule organization requires the gamma tubulin complex containing the gamma tubulin Tub4p , Spc98p , and Spc97p . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| A trimeric complex formed by Tub4p , the budding yeast gamma tubulin , and the two spindle pole body components , Spc98p and Spc97p , has recently been characterized in Saccharomyces cerevisiae . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| A genetic analysis of interactions with Spc110p reveals distinct functions of Spc97p and Spc98p , components of the yeast gamma tubulin complex . ^^^ Using the two hybrid assay , we show that the interactions of Spc110p with Spc97p and Spc98p are not equivalent . ^^^ The N terminus of Spc110p displays a robust interaction with Spc98p in two different two hybrid assays , while the interaction between Spc97p and Spc110p is not detectable in one strain and gives a weak signal in the other . ^^^ Extra copies of SPC 98 enhance the interaction between Spc97p and Spc110p , while extra copies of SPC 97 interfere with the interaction between Spc98p and Spc110p . ^^^ Moreover , an interaction between Spc98p and the amino terminal region of Spc110p is a critical component of the linkage , whereas the interaction between Spc97p and Spc110p is dependent on Spc98p . . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| Microtubule formation in yeast requires the Tub4p complex , containing the gamma tubulin Tub4p , and two additional proteins , the SPB components Spc97p and Spc98p . ^^^ This is achieved by the binding of Spc97p and Spc98p to so called gamma tubulin complex binding proteins ( GTBPs ) at the SPB . ^^^ Homologues of Spc97p and Spc98p have been identified from yeast to mammalian cells and these are also part of gamma tubulin complexes , suggesting that these related proteins may also interact with GTBPs at the centrosome . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| Cytoplasmic gamma tubulin complexes are targeted to centrosomes or to other microtubule organizing centers ( MTOCs ) via a set of so called gamma tubulin complex binding proteins ( GTBPs ) that probably interact with the conserved Spc97p / Spc98p protein family of gamma tubulin complexes . ^^^ Recently , major advances have been achieved through the finding that gamma tubulin , Spc97p and Spc98p form a conserved core that is probably responsible for microtubule nucleation , and by the discovery that a yeast GTBP is regulated in a cell cycle dependent manner and in response to an external signal . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| The recombinant complex has the same sedimentation coefficient ( 11 . 6 S ) as the native complex in yeast cell extracts and contains one molecule of Spc97p , one molecule of Spc98p , and two molecules of Tub4p . ^^^ |
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| Interacting proteins: Q96CW5 and Q9BSJ2 |
Pubmed |
SVM Score :0.0 |
| These components gamma tubulin , Spc98p , and Spc97p are present in higher plants . ^^^ |
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